Rroxscaffold_2G00093560

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007082
Physical Location & Seq
Forward (+)
14913042 .. 14921145
8104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093560.1

Sequence Viewer

Length: 2481 bp
ATGAGTAGTGGTGGTGGGTTTAGGGTTTCAATCCCAAACAGTGTGAGGAAGACGATCCAGGACATCAAAGAGATCACAGGCAATCACAGCGAGGAAGAAATCTATGCAATGCTCAAGGAATGTAACATGGATCCCAACGAGACCGCGCAGAAACTTCTCCTTCAGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAGAGAAAAGAGATAGCATTTCTTTTTAGCTTTAAGCCCCAACTGATTGTCGTATATCTTGCAGAATCTGAACAACAGAGAGTCTGCAGAGCCACGGTGGAGACCTGGAATGCCGGCTGTGCTACACCTGTTTATTTTCTGGATGCTGGTGGTGGCAGAAACTCAGGTCCTGGAACTGAAAATGGACCCGCTCAGGTTGCAGAGAAAGGTGTTGTTCCCTCTTTGCCAACCACTCGTGAGACAAAAACTAAAGAGAGAAGTCCTGTAACAAGCTCTGTACCTGTGATTGTGGGTGGTCCTTCCAATGTGGCTTCAGGAACTACTACTGTTGTGCCTGCCTCGCAGTTATCTACTGGAACTAGTGCTGAAATAAGTTTGTCATTGGTTGGGGATAAATCGGGTTCTGTGGGCACAATCAAACGTGAAGTTGTGAGCTATCATCCTCCTGCAGCTTCTGAGGTTGGCAGCTCTTTAGCACAAGGAAAAACAACAAGCAAAACACAGGGAGTTGGGAAAACTCAGCCCTCTGATCTTTCACATCCTTCATCTACATCAACTCATGGCGGTTCGGTTAGTCGGCCATCATCTAATTATAGTAGCCGGTCACAACAACTAATTGGCACTCAAAAAGCAGTTGGTACCAACAAGGAGTGGAAACCAAAGCCCATAGTTTCTGCTGTTGTTCAAGGTCAAGCAACAGCTAATGTAGCTGCACCTGAGGTGCCTGCTGATTTGGTAGAGGTCTCTCCGCAGTCACAACCTGTTTCAAGTGTCCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAAGAAGCTAGAGGAATTACACCTTCCACAGCTTCCACAGCGCAAATTAGTTATTCTTCCAAATCACATACACGTTCCTGAGTCTGAACGAAACAAGTTGAGTTTTGGAAGCTTTGGGGCAACTTTTGGCGTAACGAATAGCTATGTCAGTGGTCCTGAGAGTGAGAAGAGCTCTACTCCTCGGTCTGAAACTTCTCAGGTTATTGAAGAATCTGTGGAGGAACAGTCCTCAAGCAATCAAAATGTGTTGGCAACTGCCGATGAGGGTGATTTTCCTGATCATTCGCAATCACCTACTCATATGCTTGAAAATCTATCATCTGGTGAGGGCGATGTCTCATCCAGTGCATCCCAAGGACATAATGAATCTAAGCATGACACTGTATTGACTTCTGGAGGTCATCAGCTCCCAGTGGCTAATACTTCTCCGAATTATAGTTTTGGTGTTGTACCTCCAATTTTAGGGAGTCAGCTTGCGGCATTTGAAAACTCTGAATCTCAAGCACGTGATATATCTCGGCTTCCAAGCTTTGTAGTACAACAACCACTTGACCCAGCAAGTTATTATGCCCAATTTTACCGCTCAGGTGCTGATAGTGATGGCCGTCTTTCTCCTTTTCCATCTCCTGGGGTATCAACAAAGTACAATGGCAATGTTGGAGTATTGCCTCCAAGTTCTCAGTCTCCACAAGAGGGTGGGGTTCTTTCAGCAGCGGGTCCAACACCATTGGTGACACAGGCTTCTGGGCTTCTGCAAAGCTCAATAGCTGTAACTCAGCAGCCAGTTCCTGTCTTCCGACCGCCAGCTGGGGTGCACATACCTCATTATGCTAACTACTTTCCTTACAGTCACTACTTTAACCCATTCTATGTTCCGCCTCCTATCCACCAATATTTGGGTAATGGAGCATTTCCTCAACAACCTCAAGCAGGTGGTGTGTATCCAGCTCCACCAGCAGCTGCTGCCACAGGAGTCAAATACTCACTGCCACAATACAAGGCAGGGACTAATACGGGAAACTCAAATCACATGGGAATGGCAAGTGGATATGGGCCCTATGGCTCTTCCCCAGCTGGTTATAATCCCAGTCCTGCCACGACTGCAGGAAACACTACTCCCAATGAAGATCTTAGCACATCTCAGTTCAAGGAGAGCAATGTATACATCACTGGACAGCAGAGTGAAGGTTCATCTGTATGGGTTGCTGCACCTAACCGAGAAATGCCCAGCTTAACAAGTTCATTTTACAACCTTCCTCCACAGGGTCAGCATGTGACTTTCACTCCAACGCAGGCCGGCCATGGTACCTTTGCTGGTCTCTACCATCCTGCACAAGCAGTATCAGCTGCAGCTGTCCATCCACTTCTGCAACAGTCCCAAACCATGGCTGGGGCAGTTGACATGGTGGGACCTGGGGGAAGTGTTTATCAGCAGCCTCAGCATGCACAACAAATCAACTGGCCGAGCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

826

Amino Acids

87.18

Weight (kDa)

6.1

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 11 - 70 9.3e-35 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2085
AarI CACCTGC 1 cut(s) 1925
Acc36I ACCTGC 1 cut(s) 1925
Acc65I GGTACC 2 cut(s) 836, 2309
AccB1I GGYRCC 3 cut(s) 836, 919, 2309
AccB7I CCANNNNNTGG 3 cut(s) 1631, 1900, 2389
AccBSI CCGCTC 2 cut(s) 389, 1587
AccI GTMKAC 1 cut(s) 2166
AccII CGCG 1 cut(s) 146
AciI CCGC 9 cut(s) 144, 387, 762, 947, 1481, 1585, 1718, 1805, 1880
AclWI GGATC 5 cut(s) 49, 125, 138, 161, 174
AcoI YGGCCR 4 cut(s) 776, 1606, 2302, 2465
AcuI CTGAAG 2 cut(s) 146, 495
AcvI CACGTG 1 cut(s) 1511
AfaI GTAC 6 cut(s) 477, 838, 1455, 1542, 1649, 2311
AflIII ACRYGT 1 cut(s) 1075
AgsI TTSAA 8 cut(s) 30, 884, 966, 996, 1211, 1313, 1490, 2152
AhlI ACTAGT 1 cut(s) 557
AjnI CCWGG 5 cut(s) 57, 302, 367, 1630, 2416
Alw21I GWGCWC 2 cut(s) 1178, 1821
Alw26I GTCTC 7 cut(s) 134, 293, 431, 946, 1345, 1692, 2327
Alw44I GTGCAC 1 cut(s) 1817
AlwI GGATC 5 cut(s) 49, 125, 138, 161, 174
AlwNI CAGNNNCTG 7 cut(s) 266, 368, 653, 1595, 1793, 1964, 1967
AoxI GGCC 6 cut(s) 776, 1606, 2057, 2298, 2302, 2465
ApaI GGGCCC 1 cut(s) 2061
ApaLI GTGCAC 1 cut(s) 1817
Asp718I GGTACC 2 cut(s) 836, 2309
AspLEI GCGC 2 cut(s) 148, 1047
AspS9I GGNCC 8 cut(s) 365, 383, 494, 1157, 1721, 2057, 2058, 2414
AsuHPI GGTGA 4 cut(s) 1283, 1287, 1340, 1747
AvaII GGWCC 6 cut(s) 365, 383, 494, 1157, 1721, 2414
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 3 cut(s) 611, 1821, 2061
BamHI GGATCC 2 cut(s) 130, 166
BanI GGYRCC 3 cut(s) 836, 919, 2309
BanII GRGCYC 2 cut(s) 1178, 2061
BauI CACGAG 1 cut(s) 432
BbrPI CACGTG 1 cut(s) 1511
BbsI GAAGAC 2 cut(s) 56, 1789
Bbv12I GWGCWC 2 cut(s) 1178, 1821
BbvCI CCTCAGC 1 cut(s) 2442
BccI CCATC 5 cut(s) 787, 1598, 1633, 2337, 2370
BceAI ACGGC 1 cut(s) 1593
BciT130I CCWGG 5 cut(s) 59, 304, 369, 1632, 2418
BciVI GTATCC 1 cut(s) 1956
BclI TGATCA 1 cut(s) 1282
BcoDI GTCTC 7 cut(s) 134, 293, 431, 946, 1345, 1692, 2327
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 2 cut(s) 558, 1013
BfmI CTRYAG 5 cut(s) 283, 645, 1001, 2106, 2352
BfuAI ACCTGC 1 cut(s) 1925
BfuI GTATCC 1 cut(s) 1956
BglII AGATCT 1 cut(s) 2131
Bme1390I CCNGG 5 cut(s) 59, 304, 369, 1632, 2418
Bme18I GGWCC 6 cut(s) 365, 383, 494, 1157, 1721, 2414
BmgT120I GGNCC 8 cut(s) 365, 383, 494, 1157, 1721, 2057, 2058, 2414
BmiI GGNNCC 9 cut(s) 132, 168, 385, 838, 921, 1722, 2059, 2311, 2415
BmrFI CCNGG 5 cut(s) 59, 304, 369, 1632, 2418
BmrI ACTGGG 2 cut(s) 1409, 2085
BmsI GCATC 2 cut(s) 331, 1361
BmuI ACTGGG 2 cut(s) 1409, 2085
BpiI GAAGAC 2 cut(s) 56, 1789
BplI GAGNNNNNCTC 4 cut(s) 1160, 1192, 1165, 1197
BpmI CTGGAG 1 cut(s) 1419
Bpu10I CCTNAGC 3 cut(s) 390, 1588, 2442
BpuEI CTTGAG 4 cut(s) 98, 1219, 1488, 1914
BsaAI YACGTR 1 cut(s) 1511
BsaI GGTCTC 4 cut(s) 134, 293, 946, 2327
BsaJI CCNNGG 7 cut(s) 291, 1184, 1357, 1631, 2305, 2388, 2417
BsaXI ACNNNNNCTCC 4 cut(s) 1214, 1244, 2272, 2302
Bse118I RCCGGY 3 cut(s) 311, 798, 2300
Bse1I ACTGG 7 cut(s) 556, 1347, 1415, 1787, 2091, 2179, 2468
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 3 cut(s) 114, 1663, 2167
BseBI CCWGG 5 cut(s) 59, 304, 369, 1632, 2418
BseDI CCNNGG 7 cut(s) 291, 1184, 1357, 1631, 2305, 2388, 2417
BseGI GGATG 7 cut(s) 346, 637, 736, 1343, 1352, 2329, 2362
BseMI GCAATG 3 cut(s) 114, 1663, 2167
BseNI ACTGG 7 cut(s) 556, 1347, 1415, 1787, 2091, 2179, 2468
BseRI GAGGAG 1 cut(s) 1173
BseSI GKGCMC 3 cut(s) 611, 1821, 2061
BseYI CCCAGC 5 cut(s) 1558, 1811, 2074, 2231, 2393
BsgI GTGCAG 3 cut(s) 894, 2196, 2319
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 1805
BshFI GGCC 6 cut(s) 778, 1608, 2059, 2300, 2304, 2467
BshNI GGYRCC 3 cut(s) 836, 919, 2309
BsiEI CGRYCG 1 cut(s) 1805
BsiHKAI GWGCWC 2 cut(s) 1178, 1821
BsiSI CCGG 3 cut(s) 312, 799, 2301
BslFI GGGAC 3 cut(s) 2023, 2365, 2427
BsmAI GTCTC 7 cut(s) 134, 293, 431, 946, 1345, 1692, 2327
BsmFI GGGAC 3 cut(s) 2023, 2365, 2427
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 6 cut(s) 778, 1608, 2059, 2300, 2304, 2467
Bso31I GGTCTC 4 cut(s) 134, 293, 946, 2327
Bsp120I GGGCCC 1 cut(s) 2057
Bsp1286I GDGCHC 4 cut(s) 611, 1178, 1821, 2061
Bsp143I GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
Bsp19I CCATGG 2 cut(s) 2305, 2388
BspACI CCGC 9 cut(s) 144, 387, 762, 947, 1481, 1585, 1718, 1805, 1880
BspANI GGCC 6 cut(s) 778, 1608, 2059, 2300, 2304, 2467
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 9 cut(s) 132, 168, 385, 838, 921, 1722, 2059, 2311, 2415
BspMAI CTGCAG 5 cut(s) 287, 649, 1005, 2110, 2356
BspMI ACCTGC 1 cut(s) 1925
BspPI GGATC 5 cut(s) 49, 125, 138, 161, 174
BspQI GCTCTTC 2 cut(s) 1166, 2074
BspT107I GGYRCC 3 cut(s) 836, 919, 2309
BspTNI GGTCTC 4 cut(s) 134, 293, 946, 2327
BsrBI CCGCTC 2 cut(s) 389, 1587
BsrDI GCAATG 3 cut(s) 114, 1663, 2167
BsrFI RCCGGY 3 cut(s) 311, 798, 2300
BsrI ACTGG 7 cut(s) 556, 1347, 1415, 1787, 2091, 2179, 2468
BssAI RCCGGY 3 cut(s) 311, 798, 2300
BssECI CCNNGG 7 cut(s) 291, 1184, 1357, 1631, 2305, 2388, 2417
BssMI GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
BssNAI GTATAC 1 cut(s) 2167
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 3 cut(s) 1357, 2305, 2388
Bst1107I GTATAC 1 cut(s) 2167
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 5 cut(s) 59, 304, 369, 1632, 2418
Bst4CI ACNGT 7 cut(s) 41, 295, 526, 1230, 1387, 1853, 2379
Bst6I CTCTTC 2 cut(s) 1166, 2074
BstAPI GCANNNNNTGC 1 cut(s) 1967
BstBAI YACGTR 1 cut(s) 1511
BstC8I GCNNGC 8 cut(s) 313, 534, 924, 1479, 1809, 2298, 2302, 2448
BstDSI CCRYGG 3 cut(s) 291, 2305, 2388
BstENI CCTNNNNNAGG 1 cut(s) 1932
BstF5I GGATG 7 cut(s) 346, 637, 736, 1343, 1352, 2329, 2362
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 148, 1047
BstKTI GATC 7 cut(s) 57, 75, 133, 169, 730, 1285, 2134
BstMAI GTCTC 7 cut(s) 134, 293, 431, 946, 1345, 1692, 2327
BstMBI GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
BstMCI CGRYCG 1 cut(s) 1805
BstNI CCWGG 5 cut(s) 59, 304, 369, 1632, 2418
BstNSI RCATGY 2 cut(s) 2279, 2450
BstSCI CCNGG 5 cut(s) 57, 302, 367, 1630, 2416
BstSFI CTRYAG 5 cut(s) 283, 645, 1001, 2106, 2352
BstSLI GKGCMC 3 cut(s) 611, 1821, 2061
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 2 cut(s) 56, 1789
BstX2I RGATCY 3 cut(s) 130, 166, 2131
BstYI RGATCY 3 cut(s) 130, 166, 2131
BstZ17I GTATAC 1 cut(s) 2167
Bsu36I CCTNAGG 1 cut(s) 915
BsuI GTATCC 1 cut(s) 1956
BsuRI GGCC 6 cut(s) 778, 1608, 2059, 2300, 2304, 2467
BtgI CCRYGG 3 cut(s) 291, 2305, 2388
BtgZI GCGATG 1 cut(s) 1350
BtsCI GGATG 7 cut(s) 346, 637, 736, 1343, 1352, 2329, 2362
BtsI GCAGTG 1 cut(s) 1988
BtsIMutI CAGTG 7 cut(s) 46, 1159, 1354, 1383, 1422, 1988, 2172
BveI ACCTGC 1 cut(s) 1925
Cac8I GCNNGC 8 cut(s) 313, 534, 924, 1479, 1809, 2298, 2302, 2448
CaiI CAGNNNCTG 7 cut(s) 266, 368, 653, 1595, 1793, 1964, 1967
CciI TCATGA 1 cut(s) 175
CfoI GCGC 2 cut(s) 148, 1047
Cfr10I RCCGGY 3 cut(s) 311, 798, 2300
Cfr13I GGNCC 8 cut(s) 365, 383, 494, 1157, 1721, 2057, 2058, 2414
Csp6I GTAC 6 cut(s) 476, 837, 1454, 1541, 1648, 2310
CviQI GTAC 6 cut(s) 476, 837, 1454, 1541, 1648, 2310
DpnI GATC 7 cut(s) 56, 74, 132, 168, 729, 1284, 2133
DpnII GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
EaeI YGGCCR 4 cut(s) 776, 1606, 2302, 2465
Eam1104I CTCTTC 2 cut(s) 1166, 2074
EarI CTCTTC 2 cut(s) 1166, 2074
EciI GGCGGA 1 cut(s) 1869
Ecl136II GAGCTC 1 cut(s) 1176
Eco130I CCWWGG 3 cut(s) 1357, 2305, 2388
Eco24I GRGCYC 2 cut(s) 1178, 2061
Eco31I GGTCTC 4 cut(s) 134, 293, 946, 2327
Eco47I GGWCC 6 cut(s) 365, 383, 494, 1157, 1721, 2414
Eco53kI GAGCTC 1 cut(s) 1176
Eco57I CTGAAG 2 cut(s) 146, 495
Eco72I CACGTG 1 cut(s) 1511
Eco81I CCTNAGG 1 cut(s) 915
EcoICRI GAGCTC 1 cut(s) 1176
EcoNI CCTNNNNNAGG 1 cut(s) 1932
EcoO109I RGGNCCY 3 cut(s) 365, 2058, 2414
EcoRII CCWGG 5 cut(s) 57, 302, 367, 1630, 2416
EcoT14I CCWWGG 3 cut(s) 1357, 2305, 2388
EcoT38I GRGCYC 2 cut(s) 1178, 2061
ErhI CCWWGG 3 cut(s) 1357, 2305, 2388
FaqI GGGAC 3 cut(s) 2023, 2365, 2427
FauI CCCGC 2 cut(s) 394, 1711
FauNDI CATATG 1 cut(s) 1305
FbaI TGATCA 1 cut(s) 1282
FblI GTMKAC 1 cut(s) 2166
FokI GGATG 7 cut(s) 353, 624, 723, 1330, 1339, 2316, 2349
FriOI GRGCYC 2 cut(s) 1178, 2061
FseI GGCCGGCC 1 cut(s) 2304
FspBI CTAG 2 cut(s) 558, 1013
GlaI GCGC 2 cut(s) 147, 1046
GsaI CCCAGC 5 cut(s) 1562, 1815, 2078, 2235, 2397
GsuI CTGGAG 1 cut(s) 1419
HaeIII GGCC 6 cut(s) 778, 1608, 2059, 2300, 2304, 2467
HapII CCGG 3 cut(s) 312, 799, 2301
HhaI GCGC 2 cut(s) 148, 1047
Hin6I GCGC 2 cut(s) 146, 1045
HinP1I GCGC 2 cut(s) 146, 1045
HincII GTYRAC 1 cut(s) 2404
HindII GTYRAC 1 cut(s) 2404
HindIII AAGCTT 2 cut(s) 1114, 1531
HinfI GANTC 9 cut(s) 263, 279, 977, 1085, 1214, 1370, 1471, 1499, 1977
HpaII CCGG 3 cut(s) 312, 799, 2301
HphI GGTGA 4 cut(s) 1283, 1287, 1340, 1747
Hpy166II GTNNAC 3 cut(s) 1819, 2167, 2404
Hpy188I TCNGA 9 cut(s) 268, 655, 727, 982, 1090, 1192, 1434, 1498, 1802
Hpy188III TCNNGA 9 cut(s) 164, 176, 338, 434, 513, 1082, 1160, 1280, 1398
Hpy8I GTNNAC 3 cut(s) 1819, 2167, 2404
HpyAV CCTTC 6 cut(s) 170, 507, 750, 1037, 2183, 2267
HpyCH4III ACNGT 7 cut(s) 41, 295, 526, 1230, 1387, 1853, 2379
HpyCH4IV ACGT 4 cut(s) 193, 619, 1077, 1510
HpySE526I ACGT 4 cut(s) 193, 619, 1077, 1510
HspAI GCGC 2 cut(s) 146, 1045
KpnI GGTACC 2 cut(s) 840, 2313
KroI GCCGGC 2 cut(s) 311, 2300
KroNI GCCGGC 2 cut(s) 313, 2302
Ksp22I TGATCA 1 cut(s) 1282
Kzo9I GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
LguI GCTCTTC 2 cut(s) 1166, 2074
LmnI GCTCC 3 cut(s) 1416, 1910, 1957
LweI GCATC 2 cut(s) 331, 1361
MaeI CTAG 2 cut(s) 558, 1013
MaeII ACGT 4 cut(s) 193, 619, 1077, 1510
MalI GATC 7 cut(s) 56, 74, 132, 168, 729, 1284, 2133
MbiI CCGCTC 2 cut(s) 389, 1587
MboI GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
MflI RGATCY 3 cut(s) 130, 166, 2131
MhlI GDGCHC 4 cut(s) 611, 1178, 1821, 2061
MluCI AATT 7 cut(s) 787, 813, 1019, 1049, 1435, 1461, 1577
MlyI GAGTC 4 cut(s) 288, 1094, 1480, 1986
MmeI TCCRAC 4 cut(s) 1642, 1748, 1825, 2315
MroNI GCCGGC 2 cut(s) 311, 2300
MseI TTAA 3 cut(s) 231, 1863, 2237
MslI CAYNNNNRTG 2 cut(s) 2039, 2200
MspA1I CMGCKG 6 cut(s) 1718, 1811, 1964, 2078, 2351, 2357
MspI CCGG 3 cut(s) 312, 799, 2301
MspR9I CCNGG 5 cut(s) 59, 304, 369, 1632, 2418
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 5 cut(s) 59, 304, 369, 1632, 2418
MvnI CGCG 1 cut(s) 146
NaeI GCCGGC 2 cut(s) 313, 2302
NcoI CCATGG 2 cut(s) 2305, 2388
NdeI CATATG 1 cut(s) 1305
NdeII GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
NgoMIV GCCGGC 2 cut(s) 311, 2300
NlaIV GGNNCC 9 cut(s) 132, 168, 385, 838, 921, 1722, 2059, 2311, 2415
NmeAIII GCCGAG 1 cut(s) 1501
NmuCI GTSAC 6 cut(s) 194, 801, 951, 1735, 1853, 2278
NspI RCATGY 2 cut(s) 2279, 2450
PaeI GCATGC 1 cut(s) 2450
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 1925
PciSI GCTCTTC 2 cut(s) 1166, 2074
PctI GAATGC 1 cut(s) 313
PdiI GCCGGC 2 cut(s) 313, 2302
PfeI GAWTC 5 cut(s) 263, 977, 1214, 1370, 1499
PflMI CCANNNNNTGG 3 cut(s) 1631, 1900, 2389
PfoI TCCNGGA 2 cut(s) 57, 367
PleI GAGTC 4 cut(s) 287, 1093, 1479, 1985
PmaCI CACGTG 1 cut(s) 1511
PmlI CACGTG 1 cut(s) 1511
PpsI GAGTC 4 cut(s) 287, 1093, 1479, 1985
Ppu21I YACGTR 1 cut(s) 1511
PpuMI RGGWCCY 2 cut(s) 365, 2414
PsiI TTATAA 1 cut(s) 2085
Psp124BI GAGCTC 1 cut(s) 1178
Psp5II RGGWCCY 2 cut(s) 365, 2414
Psp6I CCWGG 5 cut(s) 57, 302, 367, 1630, 2416
PspCI CACGTG 1 cut(s) 1511
PspFI CCCAGC 5 cut(s) 1558, 1811, 2074, 2231, 2393
PspGI CCWGG 5 cut(s) 57, 302, 367, 1630, 2416
PspN4I GGNNCC 9 cut(s) 132, 168, 385, 838, 921, 1722, 2059, 2311, 2415
PspOMI GGGCCC 1 cut(s) 2057
PspPI GGNCC 8 cut(s) 365, 383, 494, 1157, 1721, 2057, 2058, 2414
PspPPI RGGWCCY 2 cut(s) 365, 2414
PstI CTGCAG 5 cut(s) 287, 649, 1005, 2110, 2356
PstNI CAGNNNCTG 7 cut(s) 266, 368, 653, 1595, 1793, 1964, 1967
PsuI RGATCY 3 cut(s) 130, 166, 2131
PvuII CAGCTG 5 cut(s) 1811, 1964, 2078, 2351, 2357
RigI GGCCGGCC 1 cut(s) 2304
RsaI GTAC 6 cut(s) 477, 838, 1455, 1542, 1649, 2311
RsaNI GTAC 6 cut(s) 476, 837, 1454, 1541, 1648, 2310
RseI CAYNNNNRTG 2 cut(s) 2039, 2200
SacI GAGCTC 1 cut(s) 1178
SapI GCTCTTC 2 cut(s) 1166, 2074
SaqAI TTAA 3 cut(s) 231, 1863, 2237
Sau3AI GATC 7 cut(s) 54, 72, 130, 166, 727, 1282, 2131
Sau96I GGNCC 8 cut(s) 365, 383, 494, 1157, 1721, 2057, 2058, 2414
SchI GAGTC 4 cut(s) 288, 1094, 1480, 1986
ScrFI CCNGG 5 cut(s) 59, 304, 369, 1632, 2418
SduI GDGCHC 4 cut(s) 611, 1178, 1821, 2061
SfaNI GCATC 2 cut(s) 331, 1361
SfcI CTRYAG 5 cut(s) 283, 645, 1001, 2106, 2352
SinI GGWCC 6 cut(s) 365, 383, 494, 1157, 1721, 2414
SmiMI CAYNNNNRTG 2 cut(s) 2039, 2200
SmlI CTYRAG 4 cut(s) 113, 1234, 1503, 1929
SmoI CTYRAG 4 cut(s) 113, 1234, 1503, 1929
SpeI ACTAGT 1 cut(s) 557
SphI GCATGC 1 cut(s) 2450
Sse9I AATT 7 cut(s) 787, 813, 1019, 1049, 1435, 1461, 1577
SsiI CCGC 9 cut(s) 144, 387, 762, 947, 1481, 1585, 1718, 1805, 1880
SspI AATATT 1 cut(s) 1898
SspMI CTAG 2 cut(s) 558, 1013
SstI GAGCTC 1 cut(s) 1178
StyD4I CCNGG 5 cut(s) 57, 302, 367, 1630, 2416
StyI CCWWGG 3 cut(s) 1357, 2305, 2388
TaaI ACNGT 7 cut(s) 41, 295, 526, 1230, 1387, 1853, 2379
TaiI ACGT 4 cut(s) 196, 622, 1080, 1513
TaqII GACCGA 1 cut(s) 1176
TasI AATT 7 cut(s) 787, 813, 1019, 1049, 1435, 1461, 1577
TatI WGTACW 2 cut(s) 1540, 1647
TauI GCSGC 1 cut(s) 1484
TfiI GAWTC 5 cut(s) 263, 977, 1214, 1370, 1499
Tru1I TTAA 3 cut(s) 231, 1863, 2237
Tru9I TTAA 3 cut(s) 231, 1863, 2237
TscAI CASTG 7 cut(s) 46, 1159, 1354, 1390, 1422, 1995, 2179
TseFI GTSAC 6 cut(s) 194, 801, 951, 1735, 1853, 2278
Tsp45I GTSAC 6 cut(s) 194, 801, 951, 1735, 1853, 2278
TspDTI ATGAA 6 cut(s) 164, 732, 1383, 2142, 2184, 2235
TspRI CASTG 7 cut(s) 46, 1159, 1354, 1390, 1422, 1995, 2179
Van91I CCANNNNNTGG 3 cut(s) 1631, 1900, 2389
VneI GTGCAC 1 cut(s) 1817
VpaK11BI GGWCC 6 cut(s) 365, 383, 494, 1157, 1721, 2414
XagI CCTNNNNNAGG 1 cut(s) 1932
XceI RCATGY 2 cut(s) 2279, 2450
XcmI CCANNNNNNNNNTGG 1 cut(s) 2390
XmiI GTMKAC 1 cut(s) 2166
XspI CTAG 2 cut(s) 558, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.