RchiOBHm_Chr1g0319061

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007055
Physical Location & Seq
Reverse (-)
6692429 .. 6696288
3860 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGAGTGCTACCGCGGATGGAGTGAACTACTCACCGGAGTGGTTGGTTAAGCACTCGAATGTTTCTGTTGAGGAGAAGCTTGACGAGCTTCGGCGTTTGATGGGCAAAGCCGAAGGTGATCCGTTAAGGATAGTTGGTGTAGGGGCAGGTGCTTGGGGGAGTGTTTTTGTTGCTATGCTGCAAGACACTTATGGACACTTGAGACAGAAGGCGGTGGTGAGAATATGGAGAAGATCTGGAAGGTCTGTGGATAGAGCCACAGCTGAACATTTGTTTGAAGTGATCAATGCAAGGGAAGATGTGTTGAGGAGGCTGATTAGGCGGTGCGCTTACTTGAAGTATGTCGAGGGGAGATTAGGTGACCGGATACTTTTTGCGGATGAGATATTGAGAGACGGGTTCTGTCTGAATATGATTGACACCCCTCTTTGCCCTTTGAAGGTTGTCACCAATTTGCAGGAGGCTGTGTGGGATGCTGATATTGTGGTGAATGGGCTGCCATCGACTGAAACTCGTGTCGTGTTTGAAGAAATTAGTAGGTATTGGAGGGATAGGAAAACAATGCCAATCATAGTTTCTCTTTCAAAAGGTGTTGAAGCTGAATTGGTGCCTGAACCGCGCATACTTACTCCCACTCAGATGATCCAGCGAGCAATGATCAAGTAG

Protein Analysis

221

Amino Acids

25.13

Weight (kDa)

8.45

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 146 - 201 9.4e-06 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 2 cut(s) 14, 619
AciI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
AclWI GGATC 2 cut(s) 113, 637
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 6 cut(s) 278, 337, 439, 527, 585, 596
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AleI CACNNNNGTG 1 cut(s) 37
AluBI AGCT 4 cut(s) 79, 88, 263, 599
AluI AGCT 4 cut(s) 79, 88, 263, 599
Alw26I GTCTC 2 cut(s) 196, 387
AlwI GGATC 2 cut(s) 113, 637
ApeKI GCWGC 2 cut(s) 178, 496
AspLEI GCGC 2 cut(s) 329, 621
AsuHPI GGTGA 6 cut(s) 24, 128, 229, 371, 439, 499
BanI GGYRCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 513
BbvI GCAGC 2 cut(s) 165, 483
BccI CCATC 3 cut(s) 11, 94, 508
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 2 cut(s) 282, 657
BcoDI GTCTC 2 cut(s) 196, 387
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 1 cut(s) 233
BisI GCNGC 2 cut(s) 179, 497
BlsI GCNGC 2 cut(s) 180, 498
BmiI GGNNCC 1 cut(s) 609
BmsI GCATC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 2 cut(s) 34, 363
BsaXI ACNNNNNCTCC 4 cut(s) 343, 373, 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 22, 385, 478
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 86, 322
BseXI GCAGC 2 cut(s) 165, 483
Bsh1236I CGCG 2 cut(s) 14, 619
BshNI GGYRCC 1 cut(s) 607
BsiSI CCGG 2 cut(s) 35, 364
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 196, 387
BsmBI CGTCTC 1 cut(s) 387
Bsp143I GATC 5 cut(s) 118, 233, 282, 642, 657
BspACI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 2 cut(s) 14, 619
BspLI GGNNCC 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 113, 637
BspT107I GGYRCC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 660
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 5 cut(s) 118, 233, 282, 642, 657
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 3 cut(s) 22, 385, 478
BstFNI CGCG 2 cut(s) 14, 619
BstHHI GCGC 2 cut(s) 329, 621
BstKTI GATC 5 cut(s) 121, 236, 285, 645, 660
BstMAI GTCTC 2 cut(s) 196, 387
BstMBI GATC 5 cut(s) 118, 233, 282, 642, 657
BstMWI GCNNNNNNNGC 3 cut(s) 85, 319, 616
BstPI GGTNACC 1 cut(s) 359
BstUI CGCG 2 cut(s) 14, 619
BstV1I GCAGC 2 cut(s) 165, 483
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BsuI GTATCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 3 cut(s) 22, 385, 478
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 651
CfoI GCGC 2 cut(s) 329, 621
Cfr42I CCGCGG 1 cut(s) 15
CviJI RGCY 9 cut(s) 79, 88, 110, 257, 263, 313, 464, 496, 599
CviKI_1 RGCY 9 cut(s) 79, 88, 110, 257, 263, 313, 464, 496, 599
DdeI CTNAG 1 cut(s) 636
DpnI GATC 5 cut(s) 120, 235, 284, 644, 659
DpnII GATC 5 cut(s) 118, 233, 282, 642, 657
Eco91I GGTNACC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 359
Esp3I CGTCTC 1 cut(s) 387
FaiI YATR 7 cut(s) 176, 192, 226, 342, 413, 572, 623
FbaI TGATCA 2 cut(s) 282, 657
Fnu4HI GCNGC 2 cut(s) 179, 497
FokI GGATG 3 cut(s) 29, 392, 485
Fsp4HI GCNGC 2 cut(s) 179, 497
GlaI GCGC 2 cut(s) 328, 620
GluI GCNGC 2 cut(s) 179, 497
HapII CCGG 2 cut(s) 35, 364
HhaI GCGC 2 cut(s) 329, 621
Hin6I GCGC 2 cut(s) 327, 619
HinP1I GCGC 2 cut(s) 327, 619
HindIII AAGCTT 1 cut(s) 77
HpaII CCGG 2 cut(s) 35, 364
HphI GGTGA 6 cut(s) 24, 128, 229, 371, 439, 499
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 408, 639
Hpy188III TCNNGA 1 cut(s) 237
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 4 cut(s) 107, 202, 234, 433
HpyCH4V TGCA 3 cut(s) 181, 290, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 319, 616
HpyF3I CTNAG 1 cut(s) 636
HspAI GCGC 2 cut(s) 327, 619
Ksp22I TGATCA 2 cut(s) 282, 657
KspI CCGCGG 1 cut(s) 15
Kzo9I GATC 5 cut(s) 118, 233, 282, 642, 657
LpnPI CCDG 7 cut(s) 48, 132, 222, 377, 443, 624, 659
Lsp1109I GCAGC 2 cut(s) 165, 483
LweI GCATC 1 cut(s) 463
MaeIII GTNAC 2 cut(s) 359, 445
MalI GATC 5 cut(s) 120, 235, 284, 644, 659
MboI GATC 5 cut(s) 118, 233, 282, 642, 657
MboII GAAGA 3 cut(s) 243, 308, 539
MflI RGATCY 1 cut(s) 233
MluCI AATT 3 cut(s) 451, 531, 602
MnlI CCTC 7 cut(s) 64, 300, 303, 340, 435, 454, 540
MseI TTAA 2 cut(s) 48, 125
MslI CAYNNNNRTG 3 cut(s) 37, 57, 638
MspA1I CMGCKG 2 cut(s) 14, 263
MspI CCGG 2 cut(s) 35, 364
MvnI CGCG 2 cut(s) 14, 619
MwoI GCNNNNNNNGC 3 cut(s) 85, 319, 616
NdeII GATC 5 cut(s) 118, 233, 282, 642, 657
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 2 cut(s) 359, 445
OliI CACNNNNGTG 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 137
PkrI GCNGC 2 cut(s) 180, 498
PspEI GGTNACC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 609
PsrI GAACNNNNNNTAC 2 cut(s) 606, 638
PsuI RGATCY 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 263
RseI CAYNNNNRTG 3 cut(s) 37, 57, 638
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 2 cut(s) 48, 125
SatI GCNGC 2 cut(s) 179, 497
Sau3AI GATC 5 cut(s) 118, 233, 282, 642, 657
SfaNI GCATC 1 cut(s) 463
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 37, 57, 638
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 3 cut(s) 451, 531, 602
SsiI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
TaqI TCGA 3 cut(s) 56, 345, 503
TasI AATT 3 cut(s) 451, 531, 602
Tru1I TTAA 2 cut(s) 48, 125
Tru9I TTAA 2 cut(s) 48, 125
TseFI GTSAC 2 cut(s) 359, 445
TseI GCWGC 2 cut(s) 178, 496
Tsp45I GTSAC 2 cut(s) 359, 445
TspGWI ACGGA 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.