Rorug01G0026500

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007055
Physical Location & Seq
Reverse (-)
4500006 .. 4501410
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0026500.1

Sequence Viewer

Length: 960 bp
ATGGTTCTCTTGTTACAAAAGATTGACAAACTCTACTCAAAGCTGGAGAATTACCACCACAACCACCACCACCACAATCACAACCATCAATCACAAGCCTTATCATCTTCTCTGGAGGCTTTTGAAACCCTTGTTTCCAGTTCCTTTAACAAATTGTTTTCGAGCTCGAAATCAGCAGGATCTGAAGTTCCAGCATTTCCATGGATCCAGCAGTGTTTTGAGCTCATACCCATCATCAACAATGGTTTTGCAAAGCTAGTTGTGGCCATAGACTATCCCATGACTAAATGGGAAGCTGATTCTGTGGAAGAGTATCTCAAATACACCTTGAATTTGCTAGAGTTGTGCAACTCCATCACTTGCTCTCTTTCTCATCTTGGGAAAGAGAGGCTTTCACTGTCTCATGGTTTGAATCTTGTGGAGAGTTCATCACCTTCTTTGGCTGTGGAGCATCTGAGACAAATTCAAGTCCAACCAAAGAGTTTGAGCAAGGATTTCAGATTCCAAGGAAATGAAGAACATGGGAAAGAAGGGTTTCCCATTACCAAGAAATCAGTTATTCATCAAGCTTTGGTGGTTATGGAGGGGATGGTGTTTTGGGTAAGTGGGATTTTGATGACTGGTTTGGTTGGGGATTCCAAGCCGTACTTGGAGATGAGACAATCTGGTGGAAGGTTAGTAAGTTCATTGCTTTCTGGATTGGATTTGAGTGGGGGTGAAGTAATTGTTGAGAAAAAAGGTATGTTGAAAGAGGTGAAAGAGATTAATGACTCAGTTGCAGCCCTTGTTTCTGCTATTGGGATTGGGAAAAAGACTAAAGCAGAAGCTGAGGAATTACAAAGAAGATTAGAGGTGTTTGAGAAGATGGTGGAGGGTTTGGGAAAAGAGGTCGATTGTCTTTTCAATAAGGTTTTGGCTGGAAGAAATCAACTGCTCAATGGATTTCACCAAAAGCATTAG

Protein Analysis

319

Amino Acids

35.56

Weight (kDa)

6.74

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BPS1 PF03087 100 - 313 1.3e-07 Protein BPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 187, 199, 212
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 2 cut(s) 331, 462
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 1 cut(s) 647
AgsI TTSAA 6 cut(s) 125, 331, 412, 467, 748, 904
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 7 cut(s) 43, 165, 223, 256, 296, 569, 827
AluI AGCT 7 cut(s) 43, 165, 223, 256, 296, 569, 827
Alw21I GWGCWC 2 cut(s) 167, 225
Alw26I GTCTC 3 cut(s) 405, 451, 652
AlwI GGATC 3 cut(s) 187, 199, 212
AlwNI CAGNNNCTG 2 cut(s) 182, 827
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 1 cut(s) 779
ApoI RAATTY 2 cut(s) 331, 462
AseI ATTAAT 1 cut(s) 765
Asp700I GAANNNNTTC 1 cut(s) 534
AsuHPI GGTGA 4 cut(s) 423, 728, 766, 938
BalI TGGCCA 1 cut(s) 266
BamHI GGATCC 1 cut(s) 204
BanII GRGCYC 2 cut(s) 167, 225
Bbv12I GWGCWC 2 cut(s) 167, 225
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 1 cut(s) 791
BccI CCATC 5 cut(s) 93, 239, 362, 583, 859
BceAI ACGGC 1 cut(s) 628
BcoDI GTCTC 3 cut(s) 405, 451, 652
BfaI CTAG 2 cut(s) 257, 338
BisI GCNGC 1 cut(s) 780
BlsI GCNGC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 206
BmsI GCATC 1 cut(s) 460
BpmI CTGGAG 2 cut(s) 65, 134
Bpu10I CCTNAGC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 200, 505
Bse1I ACTGG 2 cut(s) 138, 625
Bse3DI GCAATG 1 cut(s) 686
BseDI CCNNGG 2 cut(s) 200, 505
BseGI GGATG 1 cut(s) 594
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 3 cut(s) 446, 786, 819
BseNI ACTGG 2 cut(s) 138, 625
BseXI GCAGC 1 cut(s) 791
BshFI GGCC 1 cut(s) 266
BsiHKAI GWGCWC 2 cut(s) 167, 225
BsmAI GTCTC 3 cut(s) 405, 451, 652
BsnI GGCC 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 167, 225
Bsp143I GATC 2 cut(s) 179, 204
Bsp19I CCATGG 1 cut(s) 200
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 3 cut(s) 447, 785, 820
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 3 cut(s) 187, 199, 212
BsrDI GCAATG 1 cut(s) 686
BsrI ACTGG 2 cut(s) 138, 625
BssECI CCNNGG 2 cut(s) 200, 505
BssMI GATC 2 cut(s) 179, 204
BssT1I CCWWGG 2 cut(s) 200, 505
Bst4CI ACNGT 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 303
BstDEI CTNAG 3 cut(s) 455, 772, 828
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 594
BstKTI GATC 2 cut(s) 182, 207
BstMAI GTCTC 3 cut(s) 405, 451, 652
BstMBI GATC 2 cut(s) 179, 204
BstV1I GCAGC 1 cut(s) 791
BstX2I RGATCY 2 cut(s) 179, 204
BstYI RGATCY 2 cut(s) 179, 204
BsuRI GGCC 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 594
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 218, 395
CaiI CAGNNNCTG 2 cut(s) 182, 827
Csp6I GTAC 1 cut(s) 646
CviAII CATG 4 cut(s) 201, 280, 404, 521
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 3 cut(s) 455, 772, 828
DpnI GATC 2 cut(s) 181, 206
DpnII GATC 2 cut(s) 179, 204
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 2 cut(s) 165, 223
Eco130I CCWWGG 2 cut(s) 200, 505
Eco24I GRGCYC 2 cut(s) 167, 225
Eco53kI GAGCTC 2 cut(s) 165, 223
Eco57I CTGAAG 1 cut(s) 204
EcoICRI GAGCTC 2 cut(s) 165, 223
EcoT14I CCWWGG 2 cut(s) 200, 505
EcoT38I GRGCYC 2 cut(s) 167, 225
ErhI CCWWGG 2 cut(s) 200, 505
FaeI CATG 4 cut(s) 204, 283, 407, 524
FaiI YATR 8 cut(s) 202, 227, 269, 281, 405, 522, 581, 743
FalI AAGNNNNNCTT 4 cut(s) 375, 407, 632, 664
FatI CATG 4 cut(s) 200, 279, 403, 520
Fnu4HI GCNGC 1 cut(s) 780
FokI GGATG 1 cut(s) 601
FriOI GRGCYC 2 cut(s) 167, 225
Fsp4HI GCNGC 1 cut(s) 780
FspBI CTAG 2 cut(s) 257, 338
GluI GCNGC 1 cut(s) 780
GsuI CTGGAG 2 cut(s) 65, 134
HaeIII GGCC 1 cut(s) 266
Hin1II CATG 4 cut(s) 204, 283, 407, 524
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 5 cut(s) 299, 412, 501, 635, 770
HphI GGTGA 4 cut(s) 423, 728, 766, 938
Hpy188I TCNGA 3 cut(s) 184, 456, 500
Hpy188III TCNNGA 2 cut(s) 113, 696
HpyAV CCTTC 3 cut(s) 444, 524, 666
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 251, 348, 779
HpyF3I CTNAG 3 cut(s) 455, 772, 828
Hsp92II CATG 4 cut(s) 204, 283, 407, 524
Kzo9I GATC 2 cut(s) 179, 204
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 1 cut(s) 460
MaeI CTAG 2 cut(s) 257, 338
MaeIII GTNAC 1 cut(s) 12
MalI GATC 2 cut(s) 181, 206
MboI GATC 2 cut(s) 179, 204
MboII GAAGA 6 cut(s) 99, 320, 527, 855, 874, 933
MflI RGATCY 2 cut(s) 179, 204
MhlI GDGCHC 2 cut(s) 167, 225
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 6 cut(s) 49, 152, 331, 462, 723, 833
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 1 cut(s) 764
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 8 cut(s) 109, 381, 577, 745, 823, 844, 865, 880
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 1 cut(s) 266
MseI TTAA 2 cut(s) 147, 765
MslI CAYNNNNRTG 1 cut(s) 199
Msp20I TGGCCA 1 cut(s) 266
NcoI CCATGG 1 cut(s) 200
NdeII GATC 2 cut(s) 179, 204
NlaIII CATG 4 cut(s) 204, 283, 407, 524
NlaIV GGNNCC 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 4 cut(s) 299, 412, 501, 635
PkrI GCNGC 1 cut(s) 781
PleI GAGTC 1 cut(s) 764
PpsI GAGTC 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 765
Psp124BI GAGCTC 2 cut(s) 167, 225
PspN4I GGNNCC 1 cut(s) 206
PstNI CAGNNNCTG 2 cut(s) 182, 827
PsuI RGATCY 2 cut(s) 179, 204
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 199
SacI GAGCTC 2 cut(s) 167, 225
SaqAI TTAA 2 cut(s) 147, 765
SatI GCNGC 1 cut(s) 780
Sau3AI GATC 2 cut(s) 179, 204
SchI GAGTC 1 cut(s) 764
SduI GDGCHC 2 cut(s) 167, 225
SfaNI GCATC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 199
Sse9I AATT 6 cut(s) 49, 152, 331, 462, 723, 833
SspMI CTAG 2 cut(s) 257, 338
SstI GAGCTC 2 cut(s) 167, 225
StyI CCWWGG 2 cut(s) 200, 505
TaaI ACNGT 1 cut(s) 399
TaqI TCGA 3 cut(s) 161, 167, 891
TasI AATT 6 cut(s) 49, 152, 331, 462, 723, 833
TfiI GAWTC 4 cut(s) 299, 412, 501, 635
Tru1I TTAA 2 cut(s) 147, 765
Tru9I TTAA 2 cut(s) 147, 765
TscAI CASTG 2 cut(s) 218, 402
TseI GCWGC 1 cut(s) 779
TspDTI ATGAA 4 cut(s) 417, 528, 551, 675
TspRI CASTG 2 cut(s) 218, 402
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 2 cut(s) 331, 462
XcmI CCANNNNNNNNNTGG 2 cut(s) 198, 646
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 2 cut(s) 257, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.