Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0007055

Physical Location & Seq

Genomic Coordinates Forward (+)

62662438 .. 62666398

Gene Structure 3961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030507

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGAGTGCCACCGCTGATGGAGTGAAGTACTCACCCGAGTGGTTGGTTAAGCACTCGAATGTTTCTGTTGAGGAGAAGCTTGACGAGCTTCGGCGTTTGATGGGCAAAGCCGAAGGTGATCCGTTAAGGATAGTTGGTGTAGGGGCAGGTGCTTGGGGAAGTGTTTTTGTTGCTATGTTGCAAGACACTTATGGACACTTGAGAGAGAAGGTGGTGGTGAGAATATGGAGAAGATCTGGAAGGTCTGTGGATAGAGCCACAGCTGAACATTTGTTTGAAGTGATCAATGCAAGGGAAGATGTGTTGAGGAGGCTGATTAGGCGGTGCGCTTACTTGAAGTATGTCGAGGGGAGATTAGGTGACCGGACACTTTTTGCGGATGAGATATTGAGAGACGGGTTCTGTCTGAATATGATTGATACTCCTCTTTGCCCTTTGAAGGTTGTCACCAATTTGCAGGAGGCTGTGTGGGATGCTGATATTGTGGTGAATGGGCTGCCATCAACTGAAACTCGTGTCGTGTTTGAAGAAATTAGTAGGTATTGGAGGGATAGGAAAACAATGCCAATCATAGTTTCTCTTTCAAAAGGTGTTGAAGCTGAATTGGCGCCTGAACCACGCATACTTACTCCCACTCAGATGATCCAGCGAGCAACTGGTGCTCCTGTTGAAAACATTCTCTACCTTGGAGGACCCAACATTGCCTCAGAGATATACAACAATGAATATGCTAATGCTCGGTTATGTGGAGCAGAAAAGTGGAGAAAGTCAATGGCTAAGTTTTTAAGGCAGCCACACTTCATTGTGTGGGACAATGGTGACCTTGTTACTCATGAAGTTATGGGTGGCTTAAAGAATGTCTATGCCATTGGAGCTGGAATGGTGGCTGGGTTAACCAATGAGAGCGCCACCAGCAAATCTGTATACTTTGCACATTGTACATCAGAGATGATATTTATCACTCATTTGTTGGCAAAAGAACCAGAGAAGCTAGCAGGACCTTTATTGGCTGACACATATGTAACCCTGTTGAAAGGTCGTAATGCATGGTATGGACAAAAGCTGGCCAACGGGGAGTTAAGCCTTGAAATGGGTGATAGCATCAAGGGGAAGGGTATGATTCAGGGAGTCTCTGCTGTGAAAGCATTTTATGAGCTGCTTAGTGAGCCCTCTTTAAGTGTCAAACATCCTGAAGAAGATAAGCTCATTGCTCCTGTTCAGCTTTGTCCTATCTTGAGGATTCTATATAGTATACTTATAAAAAGGGAGTTCCCGCCTCAGGCTATTCTTGAAGCATTAAGGGATGAGACCATGCATGACCCTCGGGAGCGTATTGAGATTGCACAAACCCATGCTTTTTACAAGCCATTTCTTCTTGGTCAGCAGCAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0043207 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00564 ko01110 ko04011 map00564 map01110 map04011

Pfam Domains

Protein Families

NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

465

Amino Acids

52.09

Weight (kDa)

6.82

Isoelectric Point (pI)

41.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_Gly3P_dh_N	PF01210	146 - 245	4.6e-11	NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C	PF07479	274 - 423	7e-19	NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07690 AT3G07690
fragaria_vesca	FvH4_7g02020 FvH4_7g02020 FvH4_7g02190 FvH4_7g02190 FvH4_7g02190
malus_domestica	MD02G1297900.v1.1 MD07G1026300.v1.1
prunus_persica	Prupe.2G026500_v2.0.a1
pyrus_communis	pycom02g25080 pycom07g02000
rosa_chinensis	RchiOBHm_Chr1g0319021 RchiOBHm_Chr1g0319061
rosa_laevigata	RLG00000030507
rosa_multiflora	Rmu_sc0003503.1_g000006
rosa_roxburghii	Rroxscaffold_4G00328670
rosa_rugosa	Rorug01G0026400 Rorug01G0026500
rosa_samantha	Rh1AG038600 Rh1BG034800 Rh1CG037300 Rh1DG032700
rosa_wichuraiana	Rw1G002980 Rw1G003020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1259
AarI	CACCTGC	1 cut(s)	137
Acc36I	ACCTGC	1 cut(s)	137
AccB1I	GGYRCC	1 cut(s)	607
AccI	GTMKAC	2 cut(s)	924, 1252
AciI	CCGC	4 cut(s)	12, 322, 377, 1274
AclWI	GGATC	2 cut(s)	113, 637
AcoI	YGGCCR	1 cut(s)	1065
AcuI	CTGAAG	1 cut(s)	1212
AcyI	GRCGYC	1 cut(s)	608
AfaI	GTAC	2 cut(s)	29, 940
AfiI	CCNNNNNNNGG	3 cut(s)	439, 1090, 1279
AjuI	GAANNNNNNNTTGG	2 cut(s)	559, 591
AleI	CACNNNNGTG	1 cut(s)	37
Alw21I	GWGCWC	1 cut(s)	664
Alw26I	GTCTC	3 cut(s)	387, 1135, 1301
AlwI	GGATC	2 cut(s)	113, 637
Ama87I	CYCGRG	2 cut(s)	35, 1323
AoxI	GGCC	1 cut(s)	1065
ApeKI	GCWGC	5 cut(s)	496, 790, 1156, 1384, 1387
Asp700I	GAANNNNTTC	1 cut(s)	675
AspLEI	GCGC	3 cut(s)	329, 610, 908
AspS9I	GGNCC	2 cut(s)	692, 998
AsuHPI	GGTGA	8 cut(s)	24, 128, 229, 371, 439, 499, 830, 1106
AsuNHI	GCTAGC	1 cut(s)	991
AvaI	CYCGRG	2 cut(s)	35, 1323
AvaII	GGWCC	2 cut(s)	692, 998
AxyI	CCTNAGG	1 cut(s)	1278
BalI	TGGCCA	1 cut(s)	1067
BanI	GGYRCC	1 cut(s)	607
BanII	GRGCYC	1 cut(s)	1170
BauI	CACGAG	1 cut(s)	513
Bbv12I	GWGCWC	1 cut(s)	664
BbvI	GCAGC	3 cut(s)	483, 802, 1143
BccI	CCATC	3 cut(s)	11, 94, 508
BcgI	CGANNNNNNTGC	2 cut(s)	1304, 1338
BclI	TGATCA	1 cut(s)	282
BcoDI	GTCTC	3 cut(s)	387, 1135, 1301
BfaI	CTAG	1 cut(s)	992
BfoI	RGCGCY	2 cut(s)	611, 909
BfuAI	ACCTGC	1 cut(s)	137
BglII	AGATCT	1 cut(s)	233
BisI	GCNGC	5 cut(s)	497, 791, 1157, 1385, 1388
BlsI	GCNGC	5 cut(s)	498, 792, 1158, 1386, 1389
BmcAI	AGTACT	1 cut(s)	29
Bme18I	GGWCC	2 cut(s)	692, 998
BmeT110I	CYCGRG	2 cut(s)	35, 1323
BmgT120I	GGNCC	2 cut(s)	692, 998
BmiI	GGNNCC	2 cut(s)	609, 694
BmsI	GCATC	2 cut(s)	463, 1110
BmtI	GCTAGC	1 cut(s)	995
BpuEI	CTTGAG	2 cut(s)	220, 1255
BsaBI	GATNNNNATC	1 cut(s)	956
BsaHI	GRCGYC	1 cut(s)	608
BsaI	GGTCTC	1 cut(s)	1301
BsaJI	CCNNGG	2 cut(s)	685, 1322
BsaWI	WCCGGW	1 cut(s)	363
BsaXI	ACNNNNNCTCC	6 cut(s)	343, 373, 452, 482, 646, 676
Bsc4I	CCNNNNNNNGG	3 cut(s)	439, 1090, 1279
Bse1I	ACTGG	1 cut(s)	661
Bse21I	CCTNAGG	1 cut(s)	1278
Bse3DI	GCAATG	2 cut(s)	699, 1206
Bse8I	GATNNNNATC	1 cut(s)	956
BseDI	CCNNGG	2 cut(s)	685, 1322
BseGI	GGATG	4 cut(s)	385, 478, 1186, 1309
BseJI	GATNNNNATC	1 cut(s)	956
BseLI	CCNNNNNNNGG	3 cut(s)	439, 1090, 1279
BseMI	GCAATG	2 cut(s)	699, 1206
BseMII	CTCAG	3 cut(s)	650, 720, 1292
BseNI	ACTGG	1 cut(s)	661
BseRI	GAGGAG	3 cut(s)	86, 322, 414
BseXI	GCAGC	3 cut(s)	483, 802, 1143
BseYI	CCCAGC	1 cut(s)	887
BshFI	GGCC	1 cut(s)	1067
BshNI	GGYRCC	1 cut(s)	607
BsiHKAI	GWGCWC	1 cut(s)	664
BsiHKCI	CYCGRG	2 cut(s)	35, 1323
BsiSI	CCGG	1 cut(s)	364
BslFI	GGGAC	1 cut(s)	824
BslI	CCNNNNNNNGG	3 cut(s)	439, 1090, 1279
BsmAI	GTCTC	3 cut(s)	387, 1135, 1301
BsmBI	CGTCTC	1 cut(s)	387
BsmFI	GGGAC	1 cut(s)	824
BsnI	GGCC	1 cut(s)	1067
Bso31I	GGTCTC	1 cut(s)	1301
BsoBI	CYCGRG	2 cut(s)	35, 1323
Bsp1286I	GDGCHC	2 cut(s)	664, 1170
Bsp1407I	TGTACA	1 cut(s)	938
Bsp143I	GATC	4 cut(s)	118, 233, 282, 642
BspACI	CCGC	4 cut(s)	12, 322, 377, 1274
BspANI	GGCC	1 cut(s)	1067
BspCNI	CTCAG	3 cut(s)	649, 719, 1291
BspHI	TCATGA	1 cut(s)	832
BspLI	GGNNCC	2 cut(s)	609, 694
BspMI	ACCTGC	1 cut(s)	137
BspOI	GCTAGC	1 cut(s)	995
BspPI	GGATC	2 cut(s)	113, 637
BspT107I	GGYRCC	1 cut(s)	607
BspTNI	GGTCTC	1 cut(s)	1301
BsrDI	GCAATG	2 cut(s)	699, 1206
BsrGI	TGTACA	1 cut(s)	938
BsrI	ACTGG	1 cut(s)	661
BssECI	CCNNGG	2 cut(s)	685, 1322
BssMI	GATC	4 cut(s)	118, 233, 282, 642
BssNAI	GTATAC	2 cut(s)	925, 1253
BssNI	GRCGYC	1 cut(s)	608
BssSI	CACGAG	1 cut(s)	513
BssT1I	CCWWGG	1 cut(s)	685
Bst1107I	GTATAC	2 cut(s)	925, 1253
Bst2BI	CACGAG	1 cut(s)	513
BstACI	GRCGYC	1 cut(s)	608
BstAPI	GCANNNNNTGC	1 cut(s)	659
BstAUI	TGTACA	1 cut(s)	938
BstC8I	GCNNGC	3 cut(s)	651, 993, 1065
BstDEI	CTNAG	5 cut(s)	636, 706, 777, 1160, 1278
BstEII	GGTNACC	2 cut(s)	359, 818
BstF5I	GGATG	4 cut(s)	385, 478, 1186, 1309
BstH2I	RGCGCY	2 cut(s)	611, 909
BstHHI	GCGC	3 cut(s)	329, 610, 908
BstKTI	GATC	4 cut(s)	121, 236, 285, 645
BstMAI	GTCTC	3 cut(s)	387, 1135, 1301
BstMBI	GATC	4 cut(s)	118, 233, 282, 642
BstMWI	GCNNNNNNNGC	8 cut(s)	85, 319, 605, 659, 872, 912, 1142, 1165
BstPI	GGTNACC	2 cut(s)	359, 818
BstV1I	GCAGC	3 cut(s)	483, 802, 1143
BstX2I	RGATCY	1 cut(s)	233
BstYI	RGATCY	1 cut(s)	233
BstZ17I	GTATAC	2 cut(s)	925, 1253
Bsu36I	CCTNAGG	1 cut(s)	1278
BsuRI	GGCC	1 cut(s)	1067
BtsCI	GGATG	4 cut(s)	385, 478, 1186, 1309
BveI	ACCTGC	1 cut(s)	137
Cac8I	GCNNGC	3 cut(s)	651, 993, 1065
CciI	TCATGA	1 cut(s)	832
CfoI	GCGC	3 cut(s)	329, 610, 908
Cfr13I	GGNCC	2 cut(s)	692, 998
Csp6I	GTAC	2 cut(s)	28, 939
CviAII	CATG	5 cut(s)	833, 1047, 1312, 1316, 1352
CviQI	GTAC	2 cut(s)	28, 939
DdeI	CTNAG	5 cut(s)	636, 706, 777, 1160, 1278
DinI	GGCGCC	1 cut(s)	609
DpnI	GATC	4 cut(s)	120, 235, 284, 644
DpnII	GATC	4 cut(s)	118, 233, 282, 642
EaeI	YGGCCR	1 cut(s)	1065
Eco130I	CCWWGG	1 cut(s)	685
Eco24I	GRGCYC	1 cut(s)	1170
Eco31I	GGTCTC	1 cut(s)	1301
Eco47I	GGWCC	2 cut(s)	692, 998
Eco57I	CTGAAG	1 cut(s)	1212
Eco81I	CCTNAGG	1 cut(s)	1278
Eco88I	CYCGRG	2 cut(s)	35, 1323
Eco91I	GGTNACC	2 cut(s)	359, 818
EcoO109I	RGGNCCY	2 cut(s)	692, 998
EcoO65I	GGTNACC	2 cut(s)	359, 818
EcoT14I	CCWWGG	1 cut(s)	685
EcoT22I	ATGCAT	2 cut(s)	1048, 1317
EcoT38I	GRGCYC	1 cut(s)	1170
EgeI	GGCGCC	1 cut(s)	609
EheI	GGCGCC	1 cut(s)	609
ErhI	CCWWGG	1 cut(s)	685
Esp3I	CGTCTC	1 cut(s)	387
FaeI	CATG	5 cut(s)	836, 1050, 1315, 1319, 1355
FaqI	GGGAC	1 cut(s)	824
FatI	CATG	5 cut(s)	832, 1046, 1311, 1315, 1351
FauI	CCCGC	1 cut(s)	1281
FauNDI	CATATG	1 cut(s)	1018
FbaI	TGATCA	1 cut(s)	282
FblI	GTMKAC	2 cut(s)	924, 1252
Fnu4HI	GCNGC	5 cut(s)	497, 791, 1157, 1385, 1388
FokI	GGATG	4 cut(s)	392, 485, 1173, 1316
FriOI	GRGCYC	1 cut(s)	1170
Fsp4HI	GCNGC	5 cut(s)	497, 791, 1157, 1385, 1388
FspBI	CTAG	1 cut(s)	992
GlaI	GCGC	3 cut(s)	328, 609, 907
GluI	GCNGC	5 cut(s)	497, 791, 1157, 1385, 1388
GsaI	CCCAGC	1 cut(s)	891
HaeII	RGCGCY	2 cut(s)	611, 909
HaeIII	GGCC	1 cut(s)	1067
HapII	CCGG	1 cut(s)	364
HhaI	GCGC	3 cut(s)	329, 610, 908
Hin1I	GRCGYC	1 cut(s)	608
Hin1II	CATG	5 cut(s)	836, 1050, 1315, 1319, 1355
Hin6I	GCGC	3 cut(s)	327, 608, 906
HinP1I	GCGC	3 cut(s)	327, 608, 906
HincII	GTYRAC	1 cut(s)	894
HindII	GTYRAC	1 cut(s)	894
HindIII	AAGCTT	1 cut(s)	77
HinfI	GANTC	3 cut(s)	1120, 1128, 1240
HpaI	GTTAAC	1 cut(s)	894
HpaII	CCGG	1 cut(s)	364
HphI	GGTGA	8 cut(s)	24, 128, 229, 371, 439, 499, 830, 1106
Hpy166II	GTNNAC	3 cut(s)	894, 925, 1253
Hpy188I	TCNGA	5 cut(s)	408, 639, 709, 946, 1394
Hpy188III	TCNNGA	6 cut(s)	237, 833, 1190, 1234, 1289, 1325
Hpy8I	GTNNAC	3 cut(s)	894, 925, 1253
HpyAV	CCTTC	5 cut(s)	107, 202, 234, 433, 1105
HpyCH4V	TGCA	7 cut(s)	181, 290, 457, 932, 1046, 1315, 1343
HpyF10VI	GCNNNNNNNGC	8 cut(s)	85, 319, 605, 659, 872, 912, 1142, 1165
HpyF3I	CTNAG	5 cut(s)	636, 706, 777, 1160, 1278
Hsp92I	GRCGYC	1 cut(s)	608
Hsp92II	CATG	5 cut(s)	836, 1050, 1315, 1319, 1355
HspAI	GCGC	3 cut(s)	327, 608, 906
KasI	GGCGCC	1 cut(s)	607
Ksp22I	TGATCA	1 cut(s)	282
KspAI	GTTAAC	1 cut(s)	894
Kzo9I	GATC	4 cut(s)	118, 233, 282, 642
LmnI	GCTCC	5 cut(s)	667, 749, 872, 1216, 1327
Lsp1109I	GCAGC	3 cut(s)	483, 802, 1143
LweI	GCATC	2 cut(s)	463, 1110
MaeI	CTAG	1 cut(s)	992
MaeIII	GTNAC	5 cut(s)	359, 445, 818, 826, 1021
MalI	GATC	4 cut(s)	120, 235, 284, 644
MboI	GATC	4 cut(s)	118, 233, 282, 642
MboII	GAAGA	6 cut(s)	243, 308, 539, 1205, 1208, 1364
MflI	RGATCY	1 cut(s)	233
MhlI	GDGCHC	2 cut(s)	664, 1170
MlsI	TGGCCA	1 cut(s)	1067
MluCI	AATT	3 cut(s)	451, 531, 602
MluNI	TGGCCA	1 cut(s)	1067
Mly113I	GGCGCC	1 cut(s)	608
MlyI	GAGTC	1 cut(s)	1137
Mox20I	TGGCCA	1 cut(s)	1067
Mph1103I	ATGCAT	2 cut(s)	1048, 1317
MroXI	GAANNNNTTC	1 cut(s)	675
MscI	TGGCCA	1 cut(s)	1067
MseI	TTAA	8 cut(s)	48, 125, 785, 851, 893, 1079, 1175, 1298
MslI	CAYNNNNRTG	3 cut(s)	37, 57, 638
Msp20I	TGGCCA	1 cut(s)	1067
MspA1I	CMGCKG	2 cut(s)	14, 263
MspI	CCGG	1 cut(s)	364
MwoI	GCNNNNNNNGC	8 cut(s)	85, 319, 605, 659, 872, 912, 1142, 1165
NarI	GGCGCC	1 cut(s)	608
NdeI	CATATG	1 cut(s)	1018
NdeII	GATC	4 cut(s)	118, 233, 282, 642
NheI	GCTAGC	1 cut(s)	991
NlaIII	CATG	5 cut(s)	836, 1050, 1315, 1319, 1355
NlaIV	GGNNCC	2 cut(s)	609, 694
NmuCI	GTSAC	3 cut(s)	359, 445, 818
NsiI	ATGCAT	2 cut(s)	1048, 1317
OliI	CACNNNNGTG	1 cut(s)	37
PagI	TCATGA	1 cut(s)	832
PaqCI	CACCTGC	1 cut(s)	137
PdmI	GAANNNNTTC	1 cut(s)	675
PfeI	GAWTC	2 cut(s)	1120, 1240
PkrI	GCNGC	5 cut(s)	498, 792, 1158, 1386, 1389
PleI	GAGTC	1 cut(s)	1136
PluTI	GGCGCC	1 cut(s)	611
PpsI	GAGTC	1 cut(s)	1136
PpuMI	RGGWCCY	2 cut(s)	692, 998
PsiI	TTATAA	1 cut(s)	1259
Psp5II	RGGWCCY	2 cut(s)	692, 998
PspEI	GGTNACC	2 cut(s)	359, 818
PspFI	CCCAGC	1 cut(s)	887
PspN4I	GGNNCC	2 cut(s)	609, 694
PspPI	GGNCC	2 cut(s)	692, 998
PspPPI	RGGWCCY	2 cut(s)	692, 998
PsrI	GAACNNNNNNTAC	2 cut(s)	606, 638
PsuI	RGATCY	1 cut(s)	233
PvuII	CAGCTG	1 cut(s)	263
RsaI	GTAC	2 cut(s)	29, 940
RsaNI	GTAC	2 cut(s)	28, 939
RseI	CAYNNNNRTG	3 cut(s)	37, 57, 638
SaqAI	TTAA	8 cut(s)	48, 125, 785, 851, 893, 1079, 1175, 1298
SatI	GCNGC	5 cut(s)	497, 791, 1157, 1385, 1388
Sau3AI	GATC	4 cut(s)	118, 233, 282, 642
Sau96I	GGNCC	2 cut(s)	692, 998
ScaI	AGTACT	1 cut(s)	29
SchI	GAGTC	1 cut(s)	1137
SduI	GDGCHC	2 cut(s)	664, 1170
SfaNI	GCATC	2 cut(s)	463, 1110
SfoI	GGCGCC	1 cut(s)	609
SinI	GGWCC	2 cut(s)	692, 998
SmiMI	CAYNNNNRTG	3 cut(s)	37, 57, 638
SmlI	CTYRAG	2 cut(s)	199, 1234
SmoI	CTYRAG	2 cut(s)	199, 1234
Sse9I	AATT	3 cut(s)	451, 531, 602
SsiI	CCGC	4 cut(s)	12, 322, 377, 1274
SspDI	GGCGCC	1 cut(s)	607
SspMI	CTAG	1 cut(s)	992
StyI	CCWWGG	1 cut(s)	685
TaqI	TCGA	2 cut(s)	56, 345
TasI	AATT	3 cut(s)	451, 531, 602
TatI	WGTACW	2 cut(s)	27, 938
TfiI	GAWTC	2 cut(s)	1120, 1240
Tru1I	TTAA	8 cut(s)	48, 125, 785, 851, 893, 1079, 1175, 1298
Tru9I	TTAA	8 cut(s)	48, 125, 785, 851, 893, 1079, 1175, 1298
TseFI	GTSAC	3 cut(s)	359, 445, 818
TseI	GCWGC	5 cut(s)	496, 790, 1156, 1384, 1387
Tsp45I	GTSAC	3 cut(s)	359, 445, 818
TspDTI	ATGAA	3 cut(s)	738, 790, 849
TspGWI	ACGGA	1 cut(s)	111
VpaK11BI	GGWCC	2 cut(s)	692, 998
XcmI	CCANNNNNNNNNTGG	1 cut(s)	653
XmiI	GTMKAC	2 cut(s)	924, 1252
XmnI	GAANNNNTTC	1 cut(s)	675
XspI	CTAG	1 cut(s)	992
ZrmI	AGTACT	1 cut(s)	29
Zsp2I	ATGCAT	2 cut(s)	1048, 1317

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.