Rw1G003020

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007055
Physical Location & Seq
Reverse (-)
5512786 .. 5513749
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003020.1

Sequence Viewer

Length: 717 bp
ATGAGTGCTACCGCGGATGGAGTCAAGTACTCACCCGAGTGGTTGGTTAAGCACTCGAATGTTTCTGTTGAGGAGAAGCTTGACGAGCTTCGGCGTTTGATGGGCAAAGCCGAAGGTGATCCGTTGAGGATAGTTGGTGTAGGGGCAGGTGCTTGGGGAAGTGTTTTTGTTGCTATGTTGCAAGACACTTATGGACACTTGAGACAGAAGGCGGTGGTGAGAATATGGAGAAGATCTGGAAGGTCTGTGGATAGAGCCACAGCTGAACATTTGTTTGAAGTGATCAATGCAAGGGAAGATGTGTTGAGGAGGCTGATTAGGCGGTGCGCTTACTTGAAGTATGTCGAGGGGAGATTAGGTGACCGGATACTTTTTGCGGATGAGATATTGAGAGACGGGTTCTGTCTGAATATGATTGACACTCCTCTCTGCCCTTTGAAGGTTGTCACCAATTTGCAGGAGGCTGTGTGGGATGCTGATATTGTGGTGAATGGGCTGCCCTCGACTGAAACTCGTGTCGTGTTTGAGGAAATTAGTAGGTATTGGAGGGATAGGAAAACAATGCCAATCATAGTTTCTCTTTCAAAAGGTGTTGAAGCTGAATTGGCGCCTGAACCGCGCATACTTACTCCCACTCAGATGATCCAGCGAGCAATGGTTTATAATTCATGGAATGCCTGTATGATTATGGGGCATCTTTCATCAATCAGTAGTTAA

Protein Analysis

238

Amino Acids

26.96

Weight (kDa)

8.35

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 146 - 201 4.6e-06 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 663
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 2 cut(s) 14, 619
AciI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
AclWI GGATC 2 cut(s) 113, 637
AcyI GRCGYC 1 cut(s) 608
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 5 cut(s) 278, 337, 439, 585, 596
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AleI CACNNNNGTG 1 cut(s) 37
AluBI AGCT 4 cut(s) 79, 88, 263, 599
AluI AGCT 4 cut(s) 79, 88, 263, 599
Alw26I GTCTC 2 cut(s) 196, 387
AlwI GGATC 2 cut(s) 113, 637
Ama87I CYCGRG 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 496
AspLEI GCGC 3 cut(s) 329, 610, 621
AsuHPI GGTGA 6 cut(s) 24, 128, 229, 371, 439, 499
AvaI CYCGRG 1 cut(s) 35
BanI GGYRCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 513
BbvI GCAGC 1 cut(s) 483
BccI CCATC 2 cut(s) 11, 94
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 196, 387
BfoI RGCGCY 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 1 cut(s) 233
BisI GCNGC 1 cut(s) 497
BlsI GCNGC 1 cut(s) 498
BmcAI AGTACT 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 609
BmsI GCATC 2 cut(s) 463, 703
BpuEI CTTGAG 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 608
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 363
BsaXI ACNNNNNCTCC 4 cut(s) 343, 373, 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 22, 385, 478
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 650
BseRI GAGGAG 3 cut(s) 86, 322, 414
BseXI GCAGC 1 cut(s) 483
Bsh1236I CGCG 2 cut(s) 14, 619
BshNI GGYRCC 1 cut(s) 607
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 196, 387
BsmBI CGTCTC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 679
BsoBI CYCGRG 1 cut(s) 35
Bsp143I GATC 4 cut(s) 118, 233, 282, 642
BspACI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 2 cut(s) 14, 619
BspLI GGNNCC 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 113, 637
BspT107I GGYRCC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 660
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 4 cut(s) 118, 233, 282, 642
BssNI GRCGYC 1 cut(s) 608
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
BstACI GRCGYC 1 cut(s) 608
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 3 cut(s) 22, 385, 478
BstFNI CGCG 2 cut(s) 14, 619
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 3 cut(s) 329, 610, 621
BstKTI GATC 4 cut(s) 121, 236, 285, 645
BstMAI GTCTC 2 cut(s) 196, 387
BstMBI GATC 4 cut(s) 118, 233, 282, 642
BstMWI GCNNNNNNNGC 4 cut(s) 85, 319, 605, 616
BstPI GGTNACC 1 cut(s) 359
BstUI CGCG 2 cut(s) 14, 619
BstV1I GCAGC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BsuI GTATCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 3 cut(s) 22, 385, 478
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 651
CfoI GCGC 3 cut(s) 329, 610, 621
Cfr42I CCGCGG 1 cut(s) 15
Csp6I GTAC 1 cut(s) 28
CviAII CATG 1 cut(s) 669
CviJI RGCY 9 cut(s) 79, 88, 110, 257, 263, 313, 464, 496, 599
CviKI_1 RGCY 9 cut(s) 79, 88, 110, 257, 263, 313, 464, 496, 599
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 636
DinI GGCGCC 1 cut(s) 609
DpnI GATC 4 cut(s) 120, 235, 284, 644
DpnII GATC 4 cut(s) 118, 233, 282, 642
Eco88I CYCGRG 1 cut(s) 35
Eco91I GGTNACC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 359
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 1 cut(s) 672
FatI CATG 1 cut(s) 668
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 497
FokI GGATG 3 cut(s) 29, 392, 485
Fsp4HI GCNGC 1 cut(s) 497
GlaI GCGC 3 cut(s) 328, 609, 620
GluI GCNGC 1 cut(s) 497
HaeII RGCGCY 1 cut(s) 611
HapII CCGG 1 cut(s) 364
HhaI GCGC 3 cut(s) 329, 610, 621
Hin1I GRCGYC 1 cut(s) 608
Hin1II CATG 1 cut(s) 672
Hin6I GCGC 3 cut(s) 327, 608, 619
HinP1I GCGC 3 cut(s) 327, 608, 619
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 1 cut(s) 21
HpaII CCGG 1 cut(s) 364
HphI GGTGA 6 cut(s) 24, 128, 229, 371, 439, 499
Hpy188I TCNGA 2 cut(s) 408, 639
Hpy188III TCNNGA 1 cut(s) 237
HpyAV CCTTC 4 cut(s) 107, 202, 234, 433
HpyCH4V TGCA 3 cut(s) 181, 290, 457
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 319, 605, 616
HpyF3I CTNAG 1 cut(s) 636
Hsp92I GRCGYC 1 cut(s) 608
Hsp92II CATG 1 cut(s) 672
HspAI GCGC 3 cut(s) 327, 608, 619
KasI GGCGCC 1 cut(s) 607
Ksp22I TGATCA 1 cut(s) 282
KspI CCGCGG 1 cut(s) 15
Kzo9I GATC 4 cut(s) 118, 233, 282, 642
LpnPI CCDG 7 cut(s) 132, 222, 377, 443, 624, 659, 691
Lsp1109I GCAGC 1 cut(s) 483
LweI GCATC 2 cut(s) 463, 703
MaeIII GTNAC 2 cut(s) 359, 445
MalI GATC 4 cut(s) 120, 235, 284, 644
MboI GATC 4 cut(s) 118, 233, 282, 642
MboII GAAGA 2 cut(s) 243, 308
MflI RGATCY 1 cut(s) 233
MluCI AATT 4 cut(s) 451, 531, 602, 664
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 1 cut(s) 30
MseI TTAA 2 cut(s) 48, 715
MslI CAYNNNNRTG 3 cut(s) 37, 57, 638
MspA1I CMGCKG 2 cut(s) 14, 263
MspI CCGG 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 679
MvnI CGCG 2 cut(s) 14, 619
MwoI GCNNNNNNNGC 4 cut(s) 85, 319, 605, 616
NarI GGCGCC 1 cut(s) 608
NdeII GATC 4 cut(s) 118, 233, 282, 642
NlaIII CATG 1 cut(s) 672
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 2 cut(s) 359, 445
OliI CACNNNNGTG 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 137
PctI GAATGC 1 cut(s) 679
PkrI GCNGC 1 cut(s) 498
PleI GAGTC 1 cut(s) 29
PluTI GGCGCC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 29
PsiI TTATAA 1 cut(s) 663
PspEI GGTNACC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 609
PsrI GAACNNNNNNTAC 2 cut(s) 606, 638
PsuI RGATCY 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 3 cut(s) 37, 57, 638
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 2 cut(s) 48, 715
SatI GCNGC 1 cut(s) 497
Sau3AI GATC 4 cut(s) 118, 233, 282, 642
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 1 cut(s) 30
SfaNI GCATC 2 cut(s) 463, 703
SfoI GGCGCC 1 cut(s) 609
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 37, 57, 638
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 4 cut(s) 451, 531, 602, 664
SsiI CCGC 6 cut(s) 12, 14, 212, 322, 377, 617
SspDI GGCGCC 1 cut(s) 607
TaqI TCGA 3 cut(s) 56, 345, 503
TasI AATT 4 cut(s) 451, 531, 602, 664
TatI WGTACW 1 cut(s) 27
Tru1I TTAA 2 cut(s) 48, 715
Tru9I TTAA 2 cut(s) 48, 715
TseFI GTSAC 2 cut(s) 359, 445
TseI GCWGC 1 cut(s) 496
Tsp45I GTSAC 2 cut(s) 359, 445
TspDTI ATGAA 2 cut(s) 657, 690
TspGWI ACGGA 1 cut(s) 111
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.