RchiOBHm_Chr1g0319731

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008765
Physical Location & Seq
Reverse (-)
7402121 .. 7412626
10506 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4644 bp
ATGACGAAGGTGTACGGAACAGGCGCGTACGATTTCAAGCGCCACCATGTTGCCGAGTACCCGGTGGAGCTGAACCAACAGCTCGGAGACAAGCCGGTGGAGGCTAAACCCGGCGCGGCGCTGCCGAGCTCGATAACGCTGTCGGAGATTCAGAGGGACCAGCTCACTATGATTGCAGCTGCGAATTGGTCCAAAGCCGGCGGCGACGCCGGGCAGGTGAAAAAGGCGTTCGACTCGGAGCTGGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGACGGTGCCATTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTACTTGGAGAACTATCTCTTTCCCAATTTCGTTCCTGAAACGGCGACGTTCGAGCACGTTATGTCCATGATTCTCATGGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGTATGCTTTCATGACCGGAAAGATGCGTTTAAAGGATTCCTTGGAAGGGTTCTTCGACTTAAATCGGAAAGAGAATCAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCATTTCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAATTCCTGGAGATACTTGATTCCAAAGTCTTACCGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGCTGCCAATGGAATGGAACACATTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATAGAATTTCTTATTGACCTCTTGAGTCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTTGTTCCGAAATGCCACTTGAGTGCTTTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATAATGTCGGGAAACAATTGACGGATGATGAAGTGCTCCAATCTCATTATGATCGAGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGCAAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCATGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACGCTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGCCTTGAATCAACATATGAGATCCGTGAGGATATTCAGGAGGCTGTGCCACATCTGAATGCCTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAACCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAGAGCACAGGTACGATCTGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGTCTCAGAGGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAGTGGACTAATATGCCAGATCTCTTAGAAACCGTAGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTATAGAAAATATGGATCCAAGGCCTCCTTTTCGAATAAAGCTCCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCGGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGCCCCCATTGAGGACTCTGATAGTGAGAAAGAAAAATTTGTTGTTGAGGCATACACCCCTCCTGACCCAGGTCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTATCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACTGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCCGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTCCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAAGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTATGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTCGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCCCAGGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTTATGTAAAGGAAGATGCTGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCAGATTACCATGATAGAGGTGAGTCTGCCCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGTAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGGTTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTACGAACTTCAGCCTACCTTCCAACTTTTACTTCTGAGACCTGATCGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGATGCATTGGGAGAGCAAACAAGTCAGCAAACTGGTATGTCTGGGCACATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGCGCCAGACGATACACTGCAGGGAAGCAATTCAGAACTGAGCGAAGAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCGATTGAGAATCATACAAATGGGGTGACGGAAATGGAAACTAGTCTGGATGATGCACATGTGGCTCCTGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1547

Amino Acids

176.85

Weight (kDa)

5.72

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 55 - 468 2.4e-133 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 550 - 719 6.2e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 769 - 868 6.5e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 881 - 1177 1.2e-28 AAA domain
AAA_12 PF13087 1186 - 1378 6.6e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 205
Acc36I ACCTGC 2 cut(s) 205, 2845
Acc65I GGTACC 1 cut(s) 3867
AccB1I GGYRCC 4 cut(s) 298, 1898, 3867, 4459
AccB7I CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
AccBSI CCGCTC 1 cut(s) 2075
AccI GTMKAC 3 cut(s) 3042, 3269, 3859
AccII CGCG 3 cut(s) 26, 116, 3049
AclI AACGTT 1 cut(s) 3481
AclWI GGATC 6 cut(s) 867, 1334, 1594, 2408, 2421, 4629
AcoI YGGCCR 3 cut(s) 1224, 3907, 4522
AcuI CTGAAG 6 cut(s) 1392, 2372, 2881, 3284, 3365, 4145
AcyI GRCGYC 2 cut(s) 207, 4460
AflIII ACRYGT 2 cut(s) 3005, 4597
AhlI ACTAGT 1 cut(s) 4580
AjuI GAANNNNNNNTTGG 2 cut(s) 4167, 4199
AleI CACNNNNGTG 2 cut(s) 1023, 4065
Alw21I GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
AlwI GGATC 6 cut(s) 867, 1334, 1594, 2408, 2421, 4629
AlwNI CAGNNNCTG 1 cut(s) 1760
Ama87I CYCGRG 1 cut(s) 2987
ArsI GACNNNNNNTTYG 2 cut(s) 2047, 2079
AseI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
Asp700I GAANNNNTTC 3 cut(s) 1994, 2361, 4486
Asp718I GGTACC 1 cut(s) 3867
AspLEI GCGC 6 cut(s) 26, 42, 116, 121, 3051, 4462
AspS9I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
AsuC2I CCSGG 4 cut(s) 62, 111, 211, 2478
AsuII TTCGAA 1 cut(s) 2431
AsuNHI GCTAGC 1 cut(s) 2878
AvaI CYCGRG 1 cut(s) 2987
AvaII GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
AxyI CCTNAGG 1 cut(s) 614
BaeGI GKGCMC 2 cut(s) 4105, 4419
BaeI ACNNNNGTAYC 4 cut(s) 2706, 2739, 3851, 3884
BalI TGGCCA 3 cut(s) 1226, 3909, 4524
BamHI GGATCC 1 cut(s) 2413
BanI GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BanII GRGCYC 1 cut(s) 131
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 4098
BbsI GAAGAC 3 cut(s) 299, 548, 1922
Bbv12I GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
BceAI ACGGC 5 cut(s) 393, 2705, 2895, 3488, 4116
BciVI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BclI TGATCA 4 cut(s) 693, 2371, 3499, 4293
BcnI CCSGG 4 cut(s) 62, 111, 211, 2478
BcuI ACTAGT 1 cut(s) 4580
BfaI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
BfmI CTRYAG 3 cut(s) 1079, 3270, 4475
BfoI RGCGCY 3 cut(s) 43, 122, 4463
BfuAI ACCTGC 2 cut(s) 205, 2845
BfuI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BglII AGATCT 3 cut(s) 2203, 2299, 2972
BmcAI AGTACT 2 cut(s) 338, 2081
Bme18I GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
BmeT110I CYCGRG 1 cut(s) 2987
BmgT120I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
BmiI GGNNCC 9 cut(s) 158, 300, 1900, 2415, 2691, 3869, 4260, 4461, 4605
BmrI ACTGGG 1 cut(s) 3969
BmsI GCATC 8 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4582
BmtI GCTAGC 1 cut(s) 2882
BmuI ACTGGG 1 cut(s) 3969
BoxI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
BpiI GAAGAC 3 cut(s) 299, 548, 1922
BpmI CTGGAG 5 cut(s) 827, 1515, 3347, 3431, 3679
Bpu10I CCTNAGC 1 cut(s) 3195
Bpu14I TTCGAA 1 cut(s) 2431
BpuEI CTTGAG 3 cut(s) 970, 997, 1042
BpuMI CCSGG 4 cut(s) 62, 111, 211, 2478
Bsa29I ATCGAT 1 cut(s) 3112
BsaBI GATNNNNATC 1 cut(s) 4298
BsaHI GRCGYC 2 cut(s) 207, 4460
BsaI GGTCTC 2 cut(s) 3672, 4183
BsaWI WCCGGW 1 cut(s) 465
Bse118I RCCGGY 2 cut(s) 94, 197
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 2 cut(s) 302, 4154
Bse8I GATNNNNATC 1 cut(s) 4298
BseCI ATCGAT 1 cut(s) 3112
BseJI GATNNNNATC 1 cut(s) 4298
BseMI GCAATG 2 cut(s) 302, 4154
BseMII CTCAG 8 cut(s) 1550, 1931, 2090, 2631, 2651, 2774, 4178, 4487
BseRI GAGGAG 2 cut(s) 1988, 2167
BseSI GKGCMC 2 cut(s) 4105, 4419
BseYI CCCAGC 1 cut(s) 3051
BsgI GTGCAG 3 cut(s) 1908, 2136, 2823
Bsh1236I CGCG 3 cut(s) 26, 116, 3049
BshNI GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BshVI ATCGAT 1 cut(s) 3112
BsiHKAI GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
BsiHKCI CYCGRG 1 cut(s) 2987
BsiSI CCGG 7 cut(s) 62, 95, 111, 198, 210, 466, 2477
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 170, 2146
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 170, 2146
BsmI GAATGC 3 cut(s) 1642, 1828, 3594
Bso31I GGTCTC 2 cut(s) 3672, 4183
BsoBI CYCGRG 1 cut(s) 2987
Bsp119I TTCGAA 1 cut(s) 2431
Bsp1286I GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
Bsp19I CCATGG 1 cut(s) 4368
BspCNI CTCAG 8 cut(s) 1551, 1930, 2089, 2630, 2650, 2773, 4179, 4488
BspDI ATCGAT 1 cut(s) 3112
BspFNI CGCG 3 cut(s) 26, 116, 3049
BspHI TCATGA 3 cut(s) 460, 561, 1285
BspLI GGNNCC 9 cut(s) 158, 300, 1900, 2415, 2691, 3869, 4260, 4461, 4605
BspMAI CTGCAG 2 cut(s) 1083, 4479
BspMI ACCTGC 2 cut(s) 205, 2845
BspOI GCTAGC 1 cut(s) 2882
BspPI GGATC 6 cut(s) 867, 1334, 1594, 2408, 2421, 4629
BspQI GCTCTTC 3 cut(s) 1434, 1518, 2478
BspT104I TTCGAA 1 cut(s) 2431
BspT107I GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BspTNI GGTCTC 2 cut(s) 3672, 4183
BsrBI CCGCTC 1 cut(s) 2075
BsrDI GCAATG 2 cut(s) 302, 4154
BsrFI RCCGGY 2 cut(s) 94, 197
BssAI RCCGGY 2 cut(s) 94, 197
BssNAI GTATAC 1 cut(s) 3043
BssNI GRCGYC 2 cut(s) 207, 4460
BssSI CACGAG 1 cut(s) 4098
BssT1I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Bst1107I GTATAC 1 cut(s) 3043
Bst2BI CACGAG 1 cut(s) 4098
Bst6I CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
BstACI GRCGYC 2 cut(s) 207, 4460
BstAPI GCANNNNNTGC 1 cut(s) 2114
BstBI TTCGAA 1 cut(s) 2431
BstC8I GCNNGC 9 cut(s) 199, 1185, 1218, 1642, 2842, 2880, 2967, 3189, 3306
BstDSI CCRYGG 2 cut(s) 1672, 4368
BstEII GGTNACC 1 cut(s) 4058
BstENI CCTNNNNNAGG 2 cut(s) 2034, 2580
BstFNI CGCG 3 cut(s) 26, 116, 3049
BstH2I RGCGCY 3 cut(s) 43, 122, 4463
BstHHI GCGC 6 cut(s) 26, 42, 116, 121, 3051, 4462
BstNSI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
BstPAI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
BstPI GGTNACC 1 cut(s) 4058
BstSFI CTRYAG 3 cut(s) 1079, 3270, 4475
BstSLI GKGCMC 2 cut(s) 4105, 4419
BstUI CGCG 3 cut(s) 26, 116, 3049
BstV2I GAAGAC 3 cut(s) 299, 548, 1922
BstX2I RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
BstXI CCANNNNNNTGG 2 cut(s) 883, 3798
BstYI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
BstZ17I GTATAC 1 cut(s) 3043
Bsu15I ATCGAT 1 cut(s) 3112
Bsu36I CCTNAGG 1 cut(s) 614
BsuI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BsuTUI ATCGAT 1 cut(s) 3112
BtgI CCRYGG 2 cut(s) 1672, 4368
BtsI GCAGTG 3 cut(s) 2100, 4285, 4472
BtsIMutI CAGTG 9 cut(s) 607, 1433, 1723, 1998, 2100, 2288, 3844, 4285, 4472
BveI ACCTGC 2 cut(s) 205, 2845
Cac8I GCNNGC 9 cut(s) 199, 1185, 1218, 1642, 2842, 2880, 2967, 3189, 3306
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 3 cut(s) 460, 561, 1285
CfoI GCGC 6 cut(s) 26, 42, 116, 121, 3051, 4462
Cfr10I RCCGGY 2 cut(s) 94, 197
Cfr13I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
ClaI ATCGAT 1 cut(s) 3112
CseI GACGC 3 cut(s) 215, 1549, 3845
CsiI ACCWGGT 1 cut(s) 2695
CspCI CAANNNNNGTGG 4 cut(s) 2623, 2658, 2667, 2702
DinI GGCGCC 1 cut(s) 4461
DraI TTTAAA 3 cut(s) 481, 681, 1192
EaeI YGGCCR 3 cut(s) 1224, 3907, 4522
Eam1104I CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
EarI CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
EciI GGCGGA 1 cut(s) 1568
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Eco147I AGGCCT 1 cut(s) 2422
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 2 cut(s) 3672, 4183
Eco32I GATATC 2 cut(s) 3885, 4438
Eco47I GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 6 cut(s) 1392, 2372, 2881, 3284, 3365, 4145
Eco81I CCTNAGG 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 2987
Eco91I GGTNACC 1 cut(s) 4058
EcoICRI GAGCTC 1 cut(s) 129
EcoNI CCTNNNNNAGG 2 cut(s) 2034, 2580
EcoO109I RGGNCCY 3 cut(s) 2585, 3968, 4259
EcoO65I GGTNACC 1 cut(s) 4058
EcoRI GAATTC 3 cut(s) 800, 1706, 3128
EcoRV GATATC 2 cut(s) 3885, 4438
EcoT14I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
EcoT22I ATGCAT 2 cut(s) 574, 4378
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 4461
EheI GGCGCC 1 cut(s) 4461
ErhI CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Esp3I CGTCTC 1 cut(s) 254
FalI AAGNNNNNCTT 8 cut(s) 474, 506, 2410, 2442, 3116, 3148, 3188, 3220
FaqI GGGAC 2 cut(s) 170, 2146
FauI CCCGC 1 cut(s) 3180
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 4 cut(s) 693, 2371, 3499, 4293
FblI GTMKAC 3 cut(s) 3042, 3269, 3859
FriOI GRGCYC 1 cut(s) 131
FspBI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
GlaI GCGC 6 cut(s) 25, 41, 115, 120, 3050, 4461
GsaI CCCAGC 1 cut(s) 3055
GsuI CTGGAG 5 cut(s) 827, 1515, 3347, 3431, 3679
HaeII RGCGCY 3 cut(s) 43, 122, 4463
HapII CCGG 7 cut(s) 62, 95, 111, 198, 210, 466, 2477
HgaI GACGC 3 cut(s) 215, 1549, 3845
HhaI GCGC 6 cut(s) 26, 42, 116, 121, 3051, 4462
Hin1I GRCGYC 2 cut(s) 207, 4460
Hin6I GCGC 6 cut(s) 24, 40, 114, 119, 3049, 4460
HinP1I GCGC 6 cut(s) 24, 40, 114, 119, 3049, 4460
HincII GTYRAC 3 cut(s) 956, 1072, 3994
HindII GTYRAC 3 cut(s) 956, 1072, 3994
HindIII AAGCTT 1 cut(s) 3914
HpaII CCGG 7 cut(s) 62, 95, 111, 198, 210, 466, 2477
Hpy99I CGWCG 3 cut(s) 209, 385, 4543
HpyCH4IV ACGT 3 cut(s) 383, 393, 3481
HpySE526I ACGT 3 cut(s) 383, 393, 3481
Hsp92I GRCGYC 2 cut(s) 207, 4460
HspAI GCGC 6 cut(s) 24, 40, 114, 119, 3049, 4460
KasI GGCGCC 1 cut(s) 4459
KpnI GGTACC 1 cut(s) 3871
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 4 cut(s) 693, 2371, 3499, 4293
LguI GCTCTTC 3 cut(s) 1434, 1518, 2478
LweI GCATC 8 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4582
MabI ACCWGGT 1 cut(s) 2695
MaeI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
MaeII ACGT 3 cut(s) 383, 393, 3481
MaeIII GTNAC 6 cut(s) 1762, 3074, 3258, 3982, 4058, 4564
MbiI CCGCTC 1 cut(s) 2075
MfeI CAATTG 4 cut(s) 1124, 1239, 2981, 3746
MflI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
MhlI GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
MlsI TGGCCA 3 cut(s) 1226, 3909, 4524
MluNI TGGCCA 3 cut(s) 1226, 3909, 4524
Mly113I GGCGCC 1 cut(s) 4460
MlyI GAGTC 7 cut(s) 227, 752, 961, 2522, 3176, 3574, 3791
MmeI TCCRAC 5 cut(s) 123, 2623, 2919, 3162, 4198
Mox20I TGGCCA 3 cut(s) 1226, 3909, 4524
Mph1103I ATGCAT 2 cut(s) 574, 4378
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 3 cut(s) 1994, 2361, 4486
MscI TGGCCA 3 cut(s) 1226, 3909, 4524
MslI CAYNNNNRTG 9 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424, 4557
Msp20I TGGCCA 3 cut(s) 1226, 3909, 4524
MspA1I CMGCKG 4 cut(s) 179, 1180, 1757, 3275
MspI CCGG 7 cut(s) 62, 95, 111, 198, 210, 466, 2477
MteI GCGCNGCGC 1 cut(s) 117
MunI CAATTG 4 cut(s) 1124, 1239, 2981, 3746
Mva1269I GAATGC 3 cut(s) 1642, 1828, 3594
MvnI CGCG 3 cut(s) 26, 116, 3049
NaeI GCCGGC 1 cut(s) 199
NarI GGCGCC 1 cut(s) 4460
NciI CCSGG 4 cut(s) 62, 111, 211, 2478
NcoI CCATGG 1 cut(s) 4368
NdeI CATATG 1 cut(s) 1594
NgoMIV GCCGGC 1 cut(s) 197
NheI GCTAGC 1 cut(s) 2878
NlaIV GGNNCC 9 cut(s) 158, 300, 1900, 2415, 2691, 3869, 4260, 4461, 4605
NmeAIII GCCGAG 2 cut(s) 79, 150
NmuCI GTSAC 3 cut(s) 1762, 4058, 4564
NsiI ATGCAT 2 cut(s) 574, 4378
NspI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
NspV TTCGAA 1 cut(s) 2431
OliI CACNNNNGTG 2 cut(s) 1023, 4065
PagI TCATGA 3 cut(s) 460, 561, 1285
PaqCI CACCTGC 1 cut(s) 205
PasI CCCWGGG 1 cut(s) 3619
PceI AGGCCT 1 cut(s) 2422
PciI ACATGT 2 cut(s) 3005, 4597
PciSI GCTCTTC 3 cut(s) 1434, 1518, 2478
PcsI WCGNNNNNNNCGW 2 cut(s) 2908, 3478
PctI GAATGC 3 cut(s) 1642, 1828, 3594
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 3 cut(s) 1994, 2361, 4486
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
PfoI TCCNGGA 1 cut(s) 804
PleI GAGTC 7 cut(s) 227, 751, 960, 2522, 3175, 3573, 3790
PluTI GGCGCC 1 cut(s) 4463
PpsI GAGTC 7 cut(s) 227, 751, 960, 2522, 3175, 3573, 3790
PpuMI RGGWCCY 1 cut(s) 2585
PscI ACATGT 2 cut(s) 3005, 4597
PshAI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
PshBI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 3481
Psp5II RGGWCCY 1 cut(s) 2585
PspEI GGTNACC 1 cut(s) 4058
PspFI CCCAGC 1 cut(s) 3051
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 9 cut(s) 158, 300, 1900, 2415, 2691, 3869, 4260, 4461, 4605
PspPI GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
PspPPI RGGWCCY 1 cut(s) 2585
PstI CTGCAG 2 cut(s) 1083, 4479
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
PvuII CAGCTG 4 cut(s) 179, 1180, 1757, 3275
RseI CAYNNNNRTG 9 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424, 4557
SacI GAGCTC 1 cut(s) 131
SapI GCTCTTC 3 cut(s) 1434, 1518, 2478
Sau96I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
ScaI AGTACT 2 cut(s) 338, 2081
SchI GAGTC 7 cut(s) 227, 752, 961, 2522, 3176, 3574, 3791
SduI GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
SexAI ACCWGGT 1 cut(s) 2695
SfaNI GCATC 8 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4582
SfcI CTRYAG 3 cut(s) 1079, 3270, 4475
SfoI GGCGCC 1 cut(s) 4461
SfuI TTCGAA 1 cut(s) 2431
SinI GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
SmiMI CAYNNNNRTG 9 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424, 4557
SmlI CTYRAG 3 cut(s) 949, 976, 1021
SmoI CTYRAG 3 cut(s) 949, 976, 1021
SpeI ACTAGT 1 cut(s) 4580
SseBI AGGCCT 1 cut(s) 2422
SspDI GGCGCC 1 cut(s) 4459
SspI AATATT 4 cut(s) 1738, 2143, 2219, 3891
SspMI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 2422
StyI CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
TaiI ACGT 3 cut(s) 386, 396, 3484
TatI WGTACW 4 cut(s) 336, 912, 1165, 2079
TauI GCSGC 4 cut(s) 119, 204, 2279, 4131
TscAI CASTG 9 cut(s) 607, 1440, 1723, 2005, 2107, 2288, 3844, 4285, 4479
TseFI GTSAC 3 cut(s) 1762, 4058, 4564
Tsp45I GTSAC 3 cut(s) 1762, 4058, 4564
TspGWI ACGGA 9 cut(s) 30, 278, 1145, 1592, 1661, 3575, 3914, 3942, 4583
TspRI CASTG 9 cut(s) 607, 1440, 1723, 2005, 2107, 2288, 3844, 4285, 4479
Van91I CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
VpaK11BI GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
VspI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
XagI CCTNNNNNAGG 2 cut(s) 2034, 2580
XceI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 4375
XmiI GTMKAC 3 cut(s) 3042, 3269, 3859
XmnI GAANNNNTTC 3 cut(s) 1994, 2361, 4486
XspI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
ZrmI AGTACT 2 cut(s) 338, 2081
Zsp2I ATGCAT 2 cut(s) 574, 4378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.