RchiOBHm_Chr1g0327431

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008545
Physical Location & Seq
Reverse (-)
16258881 .. 16261176
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGCATGTTTGTACAGACGTGAGACCTGCATTGGATAAGGAACTTCCATCCGCATATGTTGAGGAATACAGGTGTGCTTTGGATGGAGAAATACTTAGATATGTGGGTGAAGCTTATCCAAAAGGGGTCTGCTCAGTGTATTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCGTGGTAGTGATTTCAGCTGCTAGACATAGCCCACAAAATTTCTGCAATGGTAGTTGGCGTTCAGTGTGGAGCATTGAGTTCAAAGATGAAGTACAAATGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGGAGGGGAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATTCAACAATTTTTCAGTCCTCTGAAGATTCTGCAATTACCATAGGCAACATTATAAGGCAACATGAGGCAGAATACTTGGCATCTCTTGAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGATCTTCGTAGAAAGCTTCTAGTTACTCGCACCCTATTTCCATGGCAGAATACTTTACAGTTTATATTGACAAGAGATATCACAAAAGAACTTGGCATTGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

202

Amino Acids

22.76

Weight (kDa)

5.18

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 14 - 192 3.3e-49 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 2 cut(s) 34, 301
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 1 cut(s) 51
AclWI GGATC 2 cut(s) 181, 510
AcsI RAATTY 1 cut(s) 226
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 2 cut(s) 13, 282
AgsI TTSAA 3 cut(s) 271, 378, 477
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 1 cut(s) 169
AjuI GAANNNNNNNTTGG 1 cut(s) 582
AluBI AGCT 4 cut(s) 113, 187, 206, 517
AluI AGCT 4 cut(s) 113, 187, 206, 517
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 2 cut(s) 181, 510
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 1 cut(s) 166
ApaI GGGCCC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 166, 167
AsuHPI GGTGA 1 cut(s) 119
BaeGI GKGCMC 2 cut(s) 170, 322
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 2 cut(s) 170, 189
BauI CACGAG 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 55, 77
BciT130I CCWGG 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 2 cut(s) 210, 521
BfmI CTRYAG 1 cut(s) 308
BfuAI ACCTGC 2 cut(s) 34, 301
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 171
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 166, 167
BmiI GGNNCC 2 cut(s) 168, 319
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 4 cut(s) 148, 343, 464, 476
BpuEI CTTGAG 1 cut(s) 482
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 169, 542
Bse3DI GCAATG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 169, 542
BseGI GGATG 3 cut(s) 47, 88, 467
BseMI GCAATG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 2 cut(s) 170, 322
BseXI GCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 16
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 16
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 3 cut(s) 170, 189, 322
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 2 cut(s) 173, 502
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 168, 319
BspMAI CTGCAG 1 cut(s) 312
BspMI ACCTGC 2 cut(s) 34, 301
BspPI GGATC 2 cut(s) 181, 510
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 241
BsrGI TGTACA 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 169, 542
BssMI GATC 2 cut(s) 173, 502
BssSI CACGAG 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 561
BstAUI TGTACA 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 95, 133
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 3 cut(s) 47, 88, 467
BstKTI GATC 2 cut(s) 176, 505
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 2 cut(s) 173, 502
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 308
BstSLI GKGCMC 2 cut(s) 170, 322
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 173, 502
BstYI RGATCY 2 cut(s) 173, 502
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 542
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 3 cut(s) 47, 88, 467
BtsIMutI CAGTG 2 cut(s) 141, 258
BveI ACCTGC 2 cut(s) 34, 301
CaiI CAGNNNCTG 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 166, 167
Csp6I GTAC 2 cut(s) 12, 281
CviAII CATG 4 cut(s) 5, 362, 437, 543
CviJI RGCY 6 cut(s) 113, 168, 187, 206, 219, 517
CviKI_1 RGCY 6 cut(s) 113, 168, 187, 206, 219, 517
CviQI GTAC 2 cut(s) 12, 281
DdeI CTNAG 2 cut(s) 95, 133
DpnI GATC 2 cut(s) 175, 504
DpnII GATC 2 cut(s) 173, 502
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 2 cut(s) 170, 189
Eco31I GGTCTC 1 cut(s) 16
Eco32I GATATC 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 169
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 542
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 170, 189
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 4 cut(s) 8, 365, 440, 546
FatI CATG 4 cut(s) 4, 361, 436, 542
FauNDI CATATG 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 3 cut(s) 34, 95, 454
FriOI GRGCYC 2 cut(s) 170, 189
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 2 cut(s) 210, 521
GluI GCNGC 1 cut(s) 207
HaeIII GGCC 1 cut(s) 168
Hin1II CATG 4 cut(s) 8, 365, 440, 546
HindIII AAGCTT 2 cut(s) 111, 515
HinfI GANTC 2 cut(s) 374, 401
HphI GGTGA 1 cut(s) 119
Hpy188I TCNGA 2 cut(s) 178, 397
Hpy188III TCNNGA 1 cut(s) 461
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 9 cut(s) 4, 29, 161, 234, 310, 346, 356, 369, 407
HpyF3I CTNAG 2 cut(s) 95, 133
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 4 cut(s) 8, 365, 440, 546
Kzo9I GATC 2 cut(s) 173, 502
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 6 cut(s) 39, 55, 156, 183, 275, 296
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 4 cut(s) 148, 343, 464, 476
MaeI CTAG 2 cut(s) 210, 521
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 523
MalI GATC 2 cut(s) 175, 504
MboI GATC 2 cut(s) 173, 502
MboII GAAGA 2 cut(s) 410, 497
MflI RGATCY 2 cut(s) 173, 502
MhlI GDGCHC 3 cut(s) 170, 189, 322
MluCI AATT 4 cut(s) 226, 293, 381, 408
MnlI CCTC 7 cut(s) 55, 157, 325, 328, 403, 433, 457
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 296, 498
MspA1I CMGCKG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 1 cut(s) 171
NcoI CCATGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 55
NdeII GATC 2 cut(s) 173, 502
NlaIII CATG 4 cut(s) 8, 365, 440, 546
NlaIV GGNNCC 2 cut(s) 168, 319
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 301
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 374, 401
PkrI GCNGC 1 cut(s) 208
PsiI TTATAA 1 cut(s) 428
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 168, 319
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 166, 167
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 2 cut(s) 173, 502
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 13, 282
RsaNI GTAC 2 cut(s) 12, 281
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 296, 498
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 2 cut(s) 173, 502
Sau96I GGNCC 2 cut(s) 166, 167
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 3 cut(s) 170, 189, 322
SetI ASST 8 cut(s) 21, 28, 74, 115, 189, 208, 315, 519
SfaNI GCATC 4 cut(s) 148, 343, 464, 476
SfcI CTRYAG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 4 cut(s) 226, 293, 381, 408
SsiI CCGC 1 cut(s) 51
SspMI CTAG 2 cut(s) 210, 521
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 1 cut(s) 561
TaiI ACGT 1 cut(s) 21
TasI AATT 4 cut(s) 226, 293, 381, 408
TatI WGTACW 2 cut(s) 11, 280
TfiI GAWTC 2 cut(s) 374, 401
Tru1I TTAA 2 cut(s) 296, 498
Tru9I TTAA 2 cut(s) 296, 498
TscAI CASTG 2 cut(s) 141, 258
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 291, 378
TspRI CASTG 2 cut(s) 141, 258
XapI RAATTY 1 cut(s) 226
XceI RCATGY 1 cut(s) 8
XspI CTAG 2 cut(s) 210, 521
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.