RchiOBHm_Chr1g0343971

retrograde vesicle-mediated transport, Golgi to ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006723
Physical Location & Seq
Reverse (-)
36331106 .. 36333802
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGGACCTCCACGCGGACAGATCCTACTCGCTCATAGATAGCCAAGAATCCATCCCATTCAAAAATGAAACTGTGATGGACCCAACACTATTGATCAAACTTGGCCGCTGGCAATGGTGGTTTTTGGTGGCAATCAACATATTCTTCCTCATTGTGGGGCAGGCTGGTGCTGTGTTACTCGGAAGATTTTATTATGACCAGGGTGGTAATAGTACGTGGATGGCTACTCTTGTGCAAACCGCAGCTTTTCCAGTTCTTGTTATCCCACTTTATTTTCTTCCTTCGCAGAAAGAAGATCAATCAACTTCTTCAACTTCACCTTCCCTTCTAGTTCTTGCCTTGATTTACTTCGTTCTTGGACTGATCATTGCTGGTGACAACATGTTGTATTCTACTGGACTCTTGTACCTATCTGCCTCTACATACTCCATCATTTCTTCAACTCAATTAGCTTTCAATGCAGTCTTCTCATACTTCATCAATTCTCAGAAGTTCAGTGCATTGATTTTCAATTCAGTGATTATCCTGACATTGTCTGTGGCTCTTATTGGTGTGAATGATGACTCGGATGGACCTGCAGGGGTATCAACGCAGAAACATATCATTGGCTTTATCTGTACCCTTGCTGCTTCTGCAATCTACTCCCTGCTACTTTCCCTTATGCAGCTTACTTTCCAGAAGGTTCTGAAAAAGGAAACTTTCTCTGTGGTTTTGCAAATGCAAATTTTTACAGCACTTGTTGCGACTTGTGCTTCAGTTGTGGGTCTTTTTGCAAGTGGGGATTGGAAGACTTTACATGGAGAAATGGAAAGTTTTGGTAAGGGAAGAGTTTCGTATGTGATGACTTTGGTGTGGACAGCTGTTTCTTGGCAAGTTTGTTCTGTTGGTGTGGTGGGTTTAATTTTCGTGGTGTCTTCCCTATTCTCGAACGTAATTAGTACTCTCTCTTTGGCTGTTACTCCTATAGCTGCTGTGATAGTTTTCCATGACAAGATGAATGGTGTGAAGGTAATTGCTATGCTAATGGCTATATGGGGTTCTGCTTCTTATATTTATCAAAACTACCTTGATGATGCCAAGGCAAGAAGAGCAGTGGCTGATGTTAGTGAATCGCACATTGCGTGA

Protein Analysis

374

Amino Acids

40.79

Weight (kDa)

5.93

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 42 - 359 2.3e-131 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccII CGCG 1 cut(s) 14
AciI CCGC 3 cut(s) 14, 106, 240
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 1 cut(s) 723
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 4 cut(s) 214, 407, 619, 940
AfiI CCNNNNNNNGG 2 cut(s) 13, 154
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 5 cut(s) 61, 312, 441, 457, 511
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 5 cut(s) 245, 452, 667, 860, 968
AluI AGCT 5 cut(s) 245, 452, 667, 860, 968
AlwI GGATC 1 cut(s) 15
AlwNI CAGNNNCTG 1 cut(s) 1097
AoxI GGCC 1 cut(s) 103
ApeKI GCWGC 4 cut(s) 242, 626, 664, 968
ApoI RAATTY 1 cut(s) 723
Asp700I GAANNNNTTC 1 cut(s) 829
AspS9I GGNCC 3 cut(s) 4, 79, 572
AsuHPI GGTGA 2 cut(s) 309, 386
AvaII GGWCC 3 cut(s) 4, 79, 572
BaeI ACNNNNGTAYC 2 cut(s) 389, 422
BbsI GAAGAC 3 cut(s) 457, 794, 906
BbvI GCAGC 4 cut(s) 254, 613, 676, 955
BccI CCATC 5 cut(s) 59, 70, 214, 437, 563
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 2 cut(s) 93, 363
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 576, 963
BfuAI ACCTGC 1 cut(s) 583
BisI GCNGC 5 cut(s) 106, 243, 627, 665, 969
BlsI GCNGC 5 cut(s) 107, 244, 628, 666, 970
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 3 cut(s) 4, 79, 572
BmgT120I GGNCC 3 cut(s) 4, 79, 572
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 1063
BpiI GAAGAC 3 cut(s) 457, 794, 906
BsaAI YACGTR 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 199, 1077
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 154
Bse1I ACTGG 2 cut(s) 251, 400
Bse3DI GCAATG 3 cut(s) 119, 366, 1116
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 199, 1077
BseGI GGATG 3 cut(s) 51, 225, 574
BseLI CCNNNNNNNGG 2 cut(s) 13, 154
BseMI GCAATG 3 cut(s) 119, 366, 1116
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 251, 400
BseXI GCAGC 4 cut(s) 254, 613, 676, 955
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 13, 154
BsnI GGCC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 20, 93, 295, 363
BspACI CCGC 3 cut(s) 14, 106, 240
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 580
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 1 cut(s) 15
BspQI GCTCTTC 1 cut(s) 1081
BsrDI GCAATG 3 cut(s) 119, 366, 1116
BsrI ACTGG 2 cut(s) 251, 400
BssECI CCNNGG 2 cut(s) 199, 1077
BssMI GATC 4 cut(s) 20, 93, 295, 363
BssT1I CCWWGG 1 cut(s) 1077
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 2 cut(s) 820, 1081
BstAPI GCANNNNNTGC 1 cut(s) 740
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 110, 162
BstDEI CTNAG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 51, 225, 574
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 4 cut(s) 23, 96, 298, 366
BstMBI GATC 4 cut(s) 20, 93, 295, 363
BstMWI GCNNNNNNNGC 5 cut(s) 458, 632, 740, 749, 1088
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 2 cut(s) 576, 963
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 4 cut(s) 254, 613, 676, 955
BstV2I GAAGAC 3 cut(s) 457, 794, 906
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 3 cut(s) 51, 225, 574
BtsI GCAGTG 1 cut(s) 1098
BtsIMutI CAGTG 3 cut(s) 502, 522, 1098
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 2 cut(s) 110, 162
CaiI CAGNNNCTG 1 cut(s) 1097
Cfr13I GGNCC 3 cut(s) 4, 79, 572
Csp6I GTAC 4 cut(s) 213, 406, 618, 939
CviAII CATG 3 cut(s) 382, 797, 986
CviQI GTAC 4 cut(s) 213, 406, 618, 939
DdeI CTNAG 1 cut(s) 486
DpnI GATC 4 cut(s) 22, 95, 297, 365
DpnII GATC 4 cut(s) 20, 93, 295, 363
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 820, 1081
EarI CTCTTC 2 cut(s) 820, 1081
Eco130I CCWWGG 1 cut(s) 1077
Eco47I GGWCC 3 cut(s) 4, 79, 572
Eco57I CTGAAG 1 cut(s) 738
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 1077
ErhI CCWWGG 1 cut(s) 1077
FaeI CATG 3 cut(s) 385, 800, 989
FatI CATG 3 cut(s) 381, 796, 985
FbaI TGATCA 2 cut(s) 93, 363
Fnu4HI GCNGC 5 cut(s) 106, 243, 627, 665, 969
FokI GGATG 3 cut(s) 38, 232, 581
Fsp4HI GCNGC 5 cut(s) 106, 243, 627, 665, 969
FspBI CTAG 1 cut(s) 329
GluI GCNGC 5 cut(s) 106, 243, 627, 665, 969
HaeIII GGCC 1 cut(s) 105
Hin1II CATG 3 cut(s) 385, 800, 989
HinfI GANTC 4 cut(s) 47, 399, 563, 1109
HphI GGTGA 2 cut(s) 309, 386
Hpy166II GTNNAC 1 cut(s) 855
Hpy188I TCNGA 4 cut(s) 182, 489, 568, 687
Hpy188III TCNNGA 3 cut(s) 526, 676, 925
Hpy8I GTNNAC 1 cut(s) 855
HpyAV CCTTC 5 cut(s) 291, 330, 335, 673, 1000
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 215, 930
HpyCH4V TGCA 9 cut(s) 235, 461, 500, 578, 635, 664, 715, 721, 773
HpyF10VI GCNNNNNNNGC 5 cut(s) 458, 632, 740, 749, 1088
HpyF3I CTNAG 1 cut(s) 486
HpySE526I ACGT 2 cut(s) 215, 930
Hsp92II CATG 3 cut(s) 385, 800, 989
Ksp22I TGATCA 2 cut(s) 93, 363
Kzo9I GATC 4 cut(s) 20, 93, 295, 363
LguI GCTCTTC 1 cut(s) 1081
Lsp1109I GCAGC 4 cut(s) 254, 613, 676, 955
LweI GCATC 1 cut(s) 1063
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 215, 930
MaeIII GTNAC 3 cut(s) 174, 374, 955
MalI GATC 4 cut(s) 22, 95, 297, 365
MboI GATC 4 cut(s) 20, 93, 295, 363
MflI RGATCY 1 cut(s) 20
MluCI AATT 7 cut(s) 446, 481, 511, 723, 900, 933, 1011
MlyI GAGTC 2 cut(s) 393, 557
MnlI CCTC 3 cut(s) 17, 158, 427
MroXI GAANNNNTTC 1 cut(s) 829
MseI TTAA 1 cut(s) 899
MspA1I CMGCKG 2 cut(s) 108, 860
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 5 cut(s) 458, 632, 740, 749, 1088
NdeII GATC 4 cut(s) 20, 93, 295, 363
NlaIII CATG 3 cut(s) 385, 800, 989
NlaIV GGNNCC 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 1 cut(s) 385
PciI ACATGT 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 1081
PcsI WCGNNNNNNNCGW 1 cut(s) 1118
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 2 cut(s) 47, 1109
PflFI GACNNNGTC 1 cut(s) 532
PkrI GCNGC 5 cut(s) 107, 244, 628, 666, 970
PleI GAGTC 2 cut(s) 393, 557
PpsI GAGTC 2 cut(s) 393, 557
Ppu21I YACGTR 1 cut(s) 216
PscI ACATGT 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 3 cut(s) 4, 79, 572
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 1097
PsuI RGATCY 1 cut(s) 20
PsyI GACNNNGTC 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 4 cut(s) 214, 407, 619, 940
RsaNI GTAC 4 cut(s) 213, 406, 618, 939
SapI GCTCTTC 1 cut(s) 1081
SaqAI TTAA 1 cut(s) 899
SatI GCNGC 5 cut(s) 106, 243, 627, 665, 969
Sau3AI GATC 4 cut(s) 20, 93, 295, 363
Sau96I GGNCC 3 cut(s) 4, 79, 572
SbfI CCTGCAGG 1 cut(s) 580
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 2 cut(s) 393, 557
ScrFI CCNGG 1 cut(s) 200
SdaI CCTGCAGG 1 cut(s) 580
SfaNI GCATC 1 cut(s) 1063
SfcI CTRYAG 2 cut(s) 576, 963
SinI GGWCC 3 cut(s) 4, 79, 572
Sse8387I CCTGCAGG 1 cut(s) 580
Sse9I AATT 7 cut(s) 446, 481, 511, 723, 900, 933, 1011
SsiI CCGC 3 cut(s) 14, 106, 240
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 2 cut(s) 218, 933
TaqI TCGA 1 cut(s) 926
TasI AATT 7 cut(s) 446, 481, 511, 723, 900, 933, 1011
TatI WGTACW 1 cut(s) 938
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 47, 1109
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 3 cut(s) 502, 522, 1098
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 4 cut(s) 242, 626, 664, 968
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 3 cut(s) 81, 466, 1010
TspRI CASTG 3 cut(s) 502, 522, 1098
Tth111I GACNNNGTC 1 cut(s) 532
VpaK11BI GGWCC 3 cut(s) 4, 79, 572
XapI RAATTY 1 cut(s) 723
XceI RCATGY 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.