RchiOBHm_Chr1g0348261

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013006
Physical Location & Seq
Forward (+)
41103607 .. 41110039
6433 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2394 bp
ATGTTGCTAAACCAAATAGTTGTAAAACAACAATCAGTCCGACGCAGAAAGACTGAGCGAGAGAGAGAGCAGAGAAGGAACAATGTCCGGTTTCAATTTCGGATCCTCCGCGGCCTCCGCTTCACAATCCACTTCCCCTTTCGGATCCTCCACCCCGGCTTTCTCATTCGCCTCCTCCCCTTCCCTTTTCGGATCCTCCGCGCCCGCCACCGCCTCCACCGGGTTCGGACTCGGATCCTCCTCTTTCGGCTCCGCTTCCTCAAACCCCTCCACCTCCTCCTCCCCTTTCAGCTTCTTATCCAACACCGCCTCCTCCCCGCTTTCAGTAACCCAAACCCTAGCCCCGCCCCGAACCCGAACCCATTTTCCTCATTCGGAGTCGGATCCTCCACCGCCTCAACCACCACCGCCGCCGCAGCTTCTACACCTTTCGGATTCAATCCCTCCACCGCCGGTTCAGGCTCCACTCCGTCGCAGCCTAAGTTCGGCTCAACCGCAACCCAATCCCTGTTTGGCTCATCCTCATCGGCGCAGGCTCCGGGTTCTACCCCATCCTTATTCGGGTCATCCGCATTCGGGTCAACTGCATCTGCCTCCGCTTCCGGCACTACCAGCTCTCTGTTCGGCTCGGCTTCGGCGGCTTCACCTTTGTTTGGTACTACTACTACTACTACTACTACTTCTTTCGCTCCCAGCACAGGCTCAGCTCTGTTTGGCTCATCCTCTTCATCGGCGGCGCCGCCCTCCTCCACCGCTCAGAACTTGTTTGGCTCTGCTCCGTCGTCCGCGGCTCCAACCACTCCCTCATTCCCAAGCTTTTCGAGCTCTTCCTCTGCAGCAGCCCCAACCACTCCCGCATTCTCAAGCTCTCTGAGCTCTTCCTCTGCAGCCCCAACCACTCCTGCATTCTCCATCTTTTTGGGCTCTTCGTCTGCAGCATCAACCACTCCCACTCCCACTCCCGCATTCCCATCCTTTTCGAGCTCTTCCGCTGCAGCCTCAACCACTTCTCCTTTCGCAGGCTTTTCGAGCTCTTCTTCTGCAGCTCCGGCCACTTCCTCGTTCGCAAGCTCTTCGGGGTTCTCATTATTCCCGAGTTCAGCTCCTGCTAAGCCCACAGCAGCATCCACACCTACTACTACGGCGCCTTCATCAAGCAGCGGTTTCAACCTCTCATTTGGAACGCCCACATCCTCGGCTTCTCAACCTTCTTTTGGATTCAGCAATGCCGCCTCATCAGCCGCAGCGGCTTCCGCCGCATTGTCATTTTCAAAGTCTTCATCGCCATTGTTTTCAACCGTCACGACCACCACGGCTTCTTCAACTCCGGCTGCAAGTAGTACTACACAATCTCCTTTCTCTTTGCCGGCTTTCAATGTGACTCCTACTGCTCCTCCTGCTGCGAGCACCACTGCACTGACTTCAACGGCAGTGCCTGCCAGCTCAGCTGCTGCTTCATCAAGCACTACTACGAGTTCCTTTACAGGTTTCTCCTTAACACCTGCTTCTTCGGCGGCTACTGCTTCTTCTTCTGGCCCTTCATTGTTTTCCAGTGCCTCTGTGCCCGCCTCGTCATCACAAGCACAACCAAGCACCACACTGCCCGCATTTGGTCTCACTACTTCAGCAACTGCAACTACCACTGCAACTACAACGACGACTGGTACCAGTACCCCTGCTGCTCCGACATCAAGTGCACTTGCTGTGGCTTCTACTAGTGGGACAACCTCAAATGTTAATGGCAGCACTACAGTATCTACGACAACACCAAAATTACCTTCTGAGATCACTGGGAAGACTGTTGAGGAGATTATCAAGGAGTGGAATGCTGAGCTTCAAGAGCGTACTGGAAAATTCCGAAAGCAGGCTAATGCAATAGCAGACTGGGACAGGAGGATTTTGCAGAACCGTGATGTTCTTCTTAGGCTCGAGATCGAAGTGGCAAATGTGGTAGATACCCAAGCTAAGGTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTCGATAAGGCTTTACAAAGCATGGAACAAGAAGCTGAGCGTATATACAAGGATGAGCGTGGATCGCTTCTTGATGATGAAGCTGCATCCACAAGAGATGCAATGTATGAGCAGGCTGAGTTTATAGAGAGGGAGCTAGAGCAGGTGACAGAGCAAATCAAATCAATTATTGAGACTGTAAATGCCAACCAGGGAGGAGAATTGGAAGTAAATGATGGGATGGCTCCACTAGATGTGGTTGTACGAATCTTAAACAACCAACTAAGCTCTTTAATGTGGATTGATGAAAAGGCCGAAGAGTTCTCATCGCGAATTCAGAAGCTTGCCAATCAAGGTCCTGCTGCAGATCGCGAATTGATGGGTCCAAGACACTGGATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

797

Amino Acids

82.48

Weight (kDa)

9.84

Isoelectric Point (pI)

58.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 587 - 690 7.3e-22 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1510, 2143
Acc36I ACCTGC 2 cut(s) 1510, 2143
Acc65I GGTACC 1 cut(s) 1664
AccB1I GGYRCC 3 cut(s) 736, 1144, 1664
AccBSI CCGCTC 2 cut(s) 755, 1975
AccII CGCG 5 cut(s) 111, 201, 788, 2320, 2361
AcoI YGGCCR 1 cut(s) 1050
AcsI RAATTY 2 cut(s) 1853, 2322
AcuI CTGAAG 1 cut(s) 1608
AcyI GRCGYC 2 cut(s) 737, 1145
AfaI GTAC 6 cut(s) 658, 1342, 1666, 1672, 1846, 2253
AgsI TTSAA 9 cut(s) 95, 439, 1168, 1272, 1296, 1323, 1375, 1425, 1838
AhlI ACTAGT 1 cut(s) 1715
AjnI CCWGG 1 cut(s) 2199
AjuI GAANNNNNNNTTGG 2 cut(s) 1762, 1794
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
Alw21I GWGCWC 6 cut(s) 827, 878, 986, 1034, 1409, 1699
Alw26I GTCTC 3 cut(s) 1619, 1988, 2177
Alw44I GTGCAC 1 cut(s) 1695
AlwNI CAGNNNCTG 4 cut(s) 1106, 1436, 1451, 1631
Ama87I CYCGRG 2 cut(s) 1093, 1928
AoxI GGCC 4 cut(s) 112, 1050, 1534, 2301
ApaLI GTGCAC 1 cut(s) 1695
ApoI RAATTY 2 cut(s) 1853, 2322
Asp718I GGTACC 1 cut(s) 1664
AspLEI GCGC 4 cut(s) 203, 532, 739, 1147
AspS9I GGNCC 3 cut(s) 1535, 2345, 2372
AsuC2I CCSGG 3 cut(s) 156, 221, 540
AsuHPI GGTGA 2 cut(s) 636, 2167
AvaI CYCGRG 2 cut(s) 1093, 1928
AvaII GGWCC 2 cut(s) 2345, 2372
BaeGI GKGCMC 2 cut(s) 1566, 1699
BaeI ACNNNNGTAYC 2 cut(s) 1737, 1770
BamHI GGATCC 5 cut(s) 102, 144, 192, 234, 383
BanI GGYRCC 3 cut(s) 736, 1144, 1664
BanII GRGCYC 5 cut(s) 827, 878, 926, 986, 1034
BbsI GAAGAC 2 cut(s) 1269, 1802
Bbv12I GWGCWC 6 cut(s) 827, 878, 986, 1034, 1409, 1699
BccI CCATC 6 cut(s) 559, 920, 979, 2219, 2224, 2362
BceAI ACGGC 3 cut(s) 1158, 1329, 1443
BcgI CGANNNNNNTGC 4 cut(s) 1008, 1042, 1056, 1090
BciT130I CCWGG 1 cut(s) 2201
BcnI CCSGG 3 cut(s) 156, 221, 540
BcoDI GTCTC 3 cut(s) 1619, 1988, 2177
BcuI ACTAGT 1 cut(s) 1715
BfaI CTAG 5 cut(s) 339, 1716, 1982, 2147, 2240
BfmI CTRYAG 7 cut(s) 834, 885, 933, 993, 1041, 1749, 2352
BfoI RGCGCY 2 cut(s) 740, 1148
BfuAI ACCTGC 2 cut(s) 1510, 2143
BglI GCCNNNNNGGC 1 cut(s) 1247
BlpI GCTNAGC 5 cut(s) 703, 1110, 1444, 1830, 2046
BmcAI AGTACT 1 cut(s) 1342
Bme1390I CCNGG 4 cut(s) 156, 221, 540, 2201
Bme18I GGWCC 2 cut(s) 2345, 2372
BmeT110I CYCGRG 2 cut(s) 1093, 1928
BmgT120I GGNCC 3 cut(s) 1535, 2345, 2372
BmrFI CCNGG 4 cut(s) 156, 221, 540, 2201
BmrI ACTGGG 2 cut(s) 1800, 1894
BmsI GCATC 5 cut(s) 596, 947, 1133, 2098, 2105
BmuI ACTGGG 2 cut(s) 1800, 1894
BpiI GAAGAC 2 cut(s) 1269, 1802
BplI GAGNNNNNCTC 2 cut(s) 1087, 1119
Bpu10I CCTNAGC 1 cut(s) 1965
Bpu1102I GCTNAGC 5 cut(s) 703, 1110, 1444, 1830, 2046
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 3 cut(s) 156, 221, 540
BsaHI GRCGYC 2 cut(s) 737, 1145
BsaI GGTCTC 1 cut(s) 1619
BsaJI CCNNGG 6 cut(s) 109, 154, 786, 1194, 1311, 2200
BsaWI WCCGGW 1 cut(s) 87
Bse118I RCCGGY 2 cut(s) 452, 1366
Bse1I ACTGG 7 cut(s) 1551, 1666, 1668, 1795, 1852, 1889, 2387
Bse3DI GCAATG 2 cut(s) 1231, 2118
BseBI CCWGG 1 cut(s) 2201
BseDI CCNNGG 6 cut(s) 109, 154, 786, 1194, 1311, 2200
BseMI GCAATG 2 cut(s) 1231, 2118
BseMII CTCAG 9 cut(s) 45, 717, 770, 863, 1458, 1773, 1821, 2037, 2118
BseNI ACTGG 7 cut(s) 1551, 1666, 1668, 1795, 1852, 1889, 2387
BseRI GAGGAG 9 cut(s) 164, 230, 266, 269, 302, 736, 1383, 1820, 2220
BseSI GKGCMC 2 cut(s) 1566, 1699
BseYI CCCAGC 1 cut(s) 692
BsgI GTGCAG 1 cut(s) 1398
Bsh1236I CGCG 5 cut(s) 111, 201, 788, 2320, 2361
BshFI GGCC 4 cut(s) 114, 1052, 1536, 2303
BshNI GGYRCC 3 cut(s) 736, 1144, 1664
BsiHKAI GWGCWC 6 cut(s) 827, 878, 986, 1034, 1409, 1699
BsiHKCI CYCGRG 2 cut(s) 1093, 1928
BsiSI CCGG 9 cut(s) 88, 156, 220, 453, 539, 603, 1049, 1328, 1367
BslFI GGGAC 2 cut(s) 1735, 1901
BsmAI GTCTC 3 cut(s) 1619, 1988, 2177
BsmFI GGGAC 2 cut(s) 1735, 1901
BsmI GAATGC 5 cut(s) 572, 857, 905, 965, 1831
BsnI GGCC 4 cut(s) 114, 1052, 1536, 2303
Bso31I GGTCTC 1 cut(s) 1619
BsoBI CYCGRG 2 cut(s) 1093, 1928
Bsp1286I GDGCHC 8 cut(s) 827, 878, 926, 986, 1034, 1409, 1566, 1699
Bsp143I GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
Bsp1720I GCTNAGC 5 cut(s) 703, 1110, 1444, 1830, 2046
Bsp68I TCGCGA 2 cut(s) 2320, 2361
BspANI GGCC 4 cut(s) 114, 1052, 1536, 2303
BspCNI CTCAG 9 cut(s) 46, 716, 769, 864, 1457, 1774, 1822, 2038, 2119
BspFNI CGCG 5 cut(s) 111, 201, 788, 2320, 2361
BspHI TCATGA 1 cut(s) 2390
BspMAI CTGCAG 6 cut(s) 838, 889, 937, 997, 1045, 2356
BspMI ACCTGC 2 cut(s) 1510, 2143
BspQI GCTCTTC 6 cut(s) 832, 883, 931, 991, 1039, 1078
BspT107I GGYRCC 3 cut(s) 736, 1144, 1664
BspTNI GGTCTC 1 cut(s) 1619
BsrBI CCGCTC 2 cut(s) 755, 1975
BsrDI GCAATG 2 cut(s) 1231, 2118
BsrFI RCCGGY 2 cut(s) 452, 1366
BsrI ACTGG 7 cut(s) 1551, 1666, 1668, 1795, 1852, 1889, 2387
BssAI RCCGGY 2 cut(s) 452, 1366
BssECI CCNNGG 6 cut(s) 109, 154, 786, 1194, 1311, 2200
BssMI GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
BssNI GRCGYC 2 cut(s) 737, 1145
Bst2UI CCWGG 1 cut(s) 2201
Bst4CI ACNGT 5 cut(s) 1300, 1753, 1801, 1910, 2188
Bst6I CTCTTC 8 cut(s) 730, 832, 883, 931, 991, 1039, 1078, 2301
BstACI GRCGYC 2 cut(s) 737, 1145
BstAPI GCANNNNNTGC 1 cut(s) 1436
BstDSI CCRYGG 3 cut(s) 109, 786, 1311
BstENI CCTNNNNNAGG 1 cut(s) 1017
BstFNI CGCG 5 cut(s) 111, 201, 788, 2320, 2361
BstH2I RGCGCY 2 cut(s) 740, 1148
BstHHI GCGC 4 cut(s) 203, 532, 739, 1147
BstKTI GATC 9 cut(s) 105, 147, 195, 237, 386, 1788, 1935, 2075, 2359
BstMAI GTCTC 3 cut(s) 1619, 1988, 2177
BstMBI GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
BstNI CCWGG 1 cut(s) 2201
BstSCI CCNGG 4 cut(s) 154, 219, 538, 2199
BstSFI CTRYAG 7 cut(s) 834, 885, 933, 993, 1041, 1749, 2352
BstSLI GKGCMC 2 cut(s) 1566, 1699
BstUI CGCG 5 cut(s) 111, 201, 788, 2320, 2361
BstV2I GAAGAC 2 cut(s) 1269, 1802
BstX2I RGATCY 5 cut(s) 102, 144, 192, 234, 383
BstXI CCANNNNNNTGG 2 cut(s) 919, 2382
BstYI RGATCY 5 cut(s) 102, 144, 192, 234, 383
BsuRI GGCC 4 cut(s) 114, 1052, 1536, 2303
BtgI CCRYGG 3 cut(s) 109, 786, 1311
BtgZI GCGATG 2 cut(s) 1266, 2301
BtsI GCAGTG 4 cut(s) 1410, 1437, 1598, 1641
BtsIMutI CAGTG 8 cut(s) 1410, 1415, 1437, 1558, 1598, 1641, 1788, 2380
BtuMI TCGCGA 2 cut(s) 2320, 2361
BveI ACCTGC 2 cut(s) 1510, 2143
CaiI CAGNNNCTG 4 cut(s) 1106, 1436, 1451, 1631
CciI TCATGA 1 cut(s) 2390
CfoI GCGC 4 cut(s) 203, 532, 739, 1147
Cfr10I RCCGGY 2 cut(s) 452, 1366
Cfr13I GGNCC 3 cut(s) 1535, 2345, 2372
Cfr42I CCGCGG 2 cut(s) 112, 789
CseI GACGC 1 cut(s) 51
Csp6I GTAC 6 cut(s) 657, 1341, 1665, 1671, 1845, 2252
CviAII CATG 2 cut(s) 2032, 2391
CviQI GTAC 6 cut(s) 657, 1341, 1665, 1671, 1845, 2252
DinI GGCGCC 2 cut(s) 738, 1146
DpnI GATC 9 cut(s) 104, 146, 194, 236, 385, 1787, 1934, 2074, 2358
DpnII GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
EaeI YGGCCR 1 cut(s) 1050
Eam1104I CTCTTC 8 cut(s) 730, 832, 883, 931, 991, 1039, 1078, 2301
EarI CTCTTC 8 cut(s) 730, 832, 883, 931, 991, 1039, 1078, 2301
EciI GGCGGA 1 cut(s) 1243
Ecl136II GAGCTC 4 cut(s) 825, 876, 984, 1032
Eco24I GRGCYC 5 cut(s) 827, 878, 926, 986, 1034
Eco31I GGTCTC 1 cut(s) 1619
Eco47I GGWCC 2 cut(s) 2345, 2372
Eco53kI GAGCTC 4 cut(s) 825, 876, 984, 1032
Eco57I CTGAAG 1 cut(s) 1608
Eco88I CYCGRG 2 cut(s) 1093, 1928
EcoICRI GAGCTC 4 cut(s) 825, 876, 984, 1032
EcoNI CCTNNNNNAGG 1 cut(s) 1017
EcoO109I RGGNCCY 1 cut(s) 2345
EcoRI GAATTC 1 cut(s) 2322
EcoRII CCWGG 1 cut(s) 2199
EcoT38I GRGCYC 5 cut(s) 827, 878, 926, 986, 1034
EgeI GGCGCC 2 cut(s) 738, 1146
EheI GGCGCC 2 cut(s) 738, 1146
FaeI CATG 2 cut(s) 2035, 2394
FaiI YATR 6 cut(s) 2033, 2054, 2056, 2118, 2135, 2392
FaqI GGGAC 2 cut(s) 1735, 1901
FatI CATG 2 cut(s) 2031, 2390
FauI CCCGC 7 cut(s) 212, 325, 352, 862, 970, 1573, 1612
FriOI GRGCYC 5 cut(s) 827, 878, 926, 986, 1034
FspBI CTAG 5 cut(s) 339, 1716, 1982, 2147, 2240
GlaI GCGC 4 cut(s) 202, 531, 738, 1146
GsaI CCCAGC 1 cut(s) 696
HaeII RGCGCY 2 cut(s) 740, 1148
HaeIII GGCC 4 cut(s) 114, 1052, 1536, 2303
HapII CCGG 9 cut(s) 88, 156, 220, 453, 539, 603, 1049, 1328, 1367
HgaI GACGC 1 cut(s) 51
HhaI GCGC 4 cut(s) 203, 532, 739, 1147
Hin1I GRCGYC 2 cut(s) 737, 1145
Hin1II CATG 2 cut(s) 2035, 2394
Hin6I GCGC 4 cut(s) 201, 530, 737, 1145
HinP1I GCGC 4 cut(s) 201, 530, 737, 1145
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HindIII AAGCTT 2 cut(s) 814, 2330
HinfI GANTC 7 cut(s) 229, 378, 435, 1218, 1381, 1996, 2256
HpaII CCGG 9 cut(s) 88, 156, 220, 453, 539, 603, 1049, 1328, 1367
HphI GGTGA 2 cut(s) 636, 2167
Hpy166II GTNNAC 2 cut(s) 582, 1697
Hpy188III TCNNGA 8 cut(s) 1093, 1303, 1838, 1930, 2081, 2319, 2360, 2391
Hpy8I GTNNAC 2 cut(s) 582, 1697
Hpy99I CGWCG 4 cut(s) 45, 475, 784, 1660
HpyAV CCTTC 6 cut(s) 69, 190, 1158, 1218, 1548, 1788
HpyCH4III ACNGT 5 cut(s) 1300, 1753, 1801, 1910, 2188
Hsp92I GRCGYC 2 cut(s) 737, 1145
Hsp92II CATG 2 cut(s) 2035, 2394
HspAI GCGC 4 cut(s) 201, 530, 737, 1145
KasI GGCGCC 2 cut(s) 736, 1144
KpnI GGTACC 1 cut(s) 1668
KroI GCCGGC 1 cut(s) 1366
KroNI GCCGGC 1 cut(s) 1368
KspI CCGCGG 2 cut(s) 112, 789
Kzo9I GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
LguI GCTCTTC 6 cut(s) 832, 883, 931, 991, 1039, 1078
LweI GCATC 5 cut(s) 596, 947, 1133, 2098, 2105
MaeI CTAG 5 cut(s) 339, 1716, 1982, 2147, 2240
MaeIII GTNAC 4 cut(s) 326, 1300, 1378, 2155
MalI GATC 9 cut(s) 104, 146, 194, 236, 385, 1787, 1934, 2074, 2358
MbiI CCGCTC 2 cut(s) 755, 1975
MboI GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
MflI RGATCY 5 cut(s) 102, 144, 192, 234, 383
MhlI GDGCHC 8 cut(s) 827, 878, 926, 986, 1034, 1409, 1566, 1699
MluCI AATT 8 cut(s) 95, 1772, 1853, 1989, 2175, 2210, 2322, 2363
Mly113I GGCGCC 2 cut(s) 737, 1145
MlyI GAGTC 4 cut(s) 223, 387, 1375, 1990
MmeI TCCRAC 5 cut(s) 64, 325, 361, 818, 1709
MroNI GCCGGC 1 cut(s) 1366
MseI TTAA 5 cut(s) 1496, 1737, 1988, 2261, 2282
MspA1I CMGCKG 6 cut(s) 111, 788, 992, 1161, 1247, 1448
MspI CCGG 9 cut(s) 88, 156, 220, 453, 539, 603, 1049, 1328, 1367
MspR9I CCNGG 4 cut(s) 156, 221, 540, 2201
Mva1269I GAATGC 5 cut(s) 572, 857, 905, 965, 1831
MvaI CCWGG 1 cut(s) 2201
MvnI CGCG 5 cut(s) 111, 201, 788, 2320, 2361
NaeI GCCGGC 1 cut(s) 1368
NarI GGCGCC 2 cut(s) 737, 1145
NciI CCSGG 3 cut(s) 156, 221, 540
NdeII GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
NgoMIV GCCGGC 1 cut(s) 1366
NlaIII CATG 2 cut(s) 2035, 2394
NmeAIII GCCGAG 2 cut(s) 608, 1175
NmuCI GTSAC 3 cut(s) 1300, 1378, 2155
NruI TCGCGA 2 cut(s) 2320, 2361
PaeR7I CTCGAG 1 cut(s) 1928
PagI TCATGA 1 cut(s) 2390
PaqCI CACCTGC 2 cut(s) 1510, 2143
PciSI GCTCTTC 6 cut(s) 832, 883, 931, 991, 1039, 1078
PctI GAATGC 5 cut(s) 572, 857, 905, 965, 1831
PdiI GCCGGC 1 cut(s) 1368
PfeI GAWTC 3 cut(s) 435, 1218, 2256
PleI GAGTC 4 cut(s) 223, 386, 1375, 1990
PluTI GGCGCC 2 cut(s) 740, 1148
PpsI GAGTC 4 cut(s) 223, 386, 1375, 1990
PpuMI RGGWCCY 1 cut(s) 2345
Psp124BI GAGCTC 4 cut(s) 827, 878, 986, 1034
Psp5II RGGWCCY 1 cut(s) 2345
Psp6I CCWGG 1 cut(s) 2199
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 1 cut(s) 2199
PspPI GGNCC 3 cut(s) 1535, 2345, 2372
PspPPI RGGWCCY 1 cut(s) 2345
PstI CTGCAG 6 cut(s) 838, 889, 937, 997, 1045, 2356
PstNI CAGNNNCTG 4 cut(s) 1106, 1436, 1451, 1631
PsuI RGATCY 5 cut(s) 102, 144, 192, 234, 383
PvuII CAGCTG 1 cut(s) 1448
RruI TCGCGA 2 cut(s) 2320, 2361
RsaI GTAC 6 cut(s) 658, 1342, 1666, 1672, 1846, 2253
RsaNI GTAC 6 cut(s) 657, 1341, 1665, 1671, 1845, 2252
SacI GAGCTC 4 cut(s) 827, 878, 986, 1034
SacII CCGCGG 2 cut(s) 112, 789
SapI GCTCTTC 6 cut(s) 832, 883, 931, 991, 1039, 1078
SaqAI TTAA 5 cut(s) 1496, 1737, 1988, 2261, 2282
Sau3AI GATC 9 cut(s) 102, 144, 192, 234, 383, 1785, 1932, 2072, 2356
Sau96I GGNCC 3 cut(s) 1535, 2345, 2372
ScaI AGTACT 1 cut(s) 1342
SchI GAGTC 4 cut(s) 223, 387, 1375, 1990
ScrFI CCNGG 4 cut(s) 156, 221, 540, 2201
SduI GDGCHC 8 cut(s) 827, 878, 926, 986, 1034, 1409, 1566, 1699
SfaNI GCATC 5 cut(s) 596, 947, 1133, 2098, 2105
SfcI CTRYAG 7 cut(s) 834, 885, 933, 993, 1041, 1749, 2352
SfoI GGCGCC 2 cut(s) 738, 1146
Sfr274I CTCGAG 1 cut(s) 1928
Sfr303I CCGCGG 2 cut(s) 112, 789
SgrBI CCGCGG 2 cut(s) 112, 789
SinI GGWCC 2 cut(s) 2345, 2372
SlaI CTCGAG 1 cut(s) 1928
SmlI CTYRAG 2 cut(s) 862, 1928
SmoI CTYRAG 2 cut(s) 862, 1928
SpeI ACTAGT 1 cut(s) 1715
Sse9I AATT 8 cut(s) 95, 1772, 1853, 1989, 2175, 2210, 2322, 2363
SspDI GGCGCC 2 cut(s) 736, 1144
SspMI CTAG 5 cut(s) 339, 1716, 1982, 2147, 2240
SstI GAGCTC 4 cut(s) 827, 878, 986, 1034
StyD4I CCNGG 4 cut(s) 154, 219, 538, 2199
TaaI ACNGT 5 cut(s) 1300, 1753, 1801, 1910, 2188
TaqI TCGA 6 cut(s) 821, 980, 1028, 1929, 1935, 2013
TasI AATT 8 cut(s) 95, 1772, 1853, 1989, 2175, 2210, 2322, 2363
TatI WGTACW 1 cut(s) 1340
TfiI GAWTC 3 cut(s) 435, 1218, 2256
Tru1I TTAA 5 cut(s) 1496, 1737, 1988, 2261, 2282
Tru9I TTAA 5 cut(s) 1496, 1737, 1988, 2261, 2282
TscAI CASTG 8 cut(s) 1417, 1422, 1437, 1558, 1605, 1648, 1795, 2387
TseFI GTSAC 3 cut(s) 1300, 1378, 2155
Tsp45I GTSAC 3 cut(s) 1300, 1378, 2155
TspDTI ATGAA 7 cut(s) 717, 1140, 1269, 1446, 1530, 2103, 2310
TspGWI ACGGA 2 cut(s) 459, 768
TspRI CASTG 8 cut(s) 1417, 1422, 1437, 1558, 1605, 1648, 1795, 2387
VneI GTGCAC 1 cut(s) 1695
VpaK11BI GGWCC 2 cut(s) 2345, 2372
XagI CCTNNNNNAGG 1 cut(s) 1017
XapI RAATTY 2 cut(s) 1853, 2322
XcmI CCANNNNNNNNNTGG 1 cut(s) 509
XhoI CTCGAG 1 cut(s) 1928
XspI CTAG 5 cut(s) 339, 1716, 1982, 2147, 2240
ZrmI AGTACT 1 cut(s) 1342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.