RchiOBHm_Chr1g0349701

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008566
Physical Location & Seq
Forward (+)
42769242 .. 42772520
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGCTTATTTTGTGTGAGAGCCTCAGACTGTTGTATATGATAATGGCGACTGCAATTACAGTGCCTACCTATCCAAGAAATGTTAAGCTTTTATCTTCTAAGTGTGTTGGTTCCAGAGTTATAGCGCTGGGTCTTTGTGCAATGTCTTACAAGCCTACTAGTACTTTTGGGCACAAGCTCAATTCTCGCTGCTGTTTCTTCCGTATGCCTGAACCTCTTCTAGTCAACAATTTCAAGAGGATTTCATGGTCTGTCAGAAGCAGTGTAGATAACAGTGGGTTAGACCCATCTCCAACAAATGGCACTACAGGCACAACCAGATTAATTAGGGTTATCCAAGCTGTTCAATCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCGTGAAAATACTTTTCTTCCTAGTCGGTTTCTATTGTTCAACTGCTTATGCTACTGTTATTGGGCAAACTGGAGACTGGGACATTCTCTCTGCGGCCTTGGCTGTGCTTATTGTGGAGGGGATTGGGGCCCTCATGTACAAGGCTTCTCTTCCTTTATTGAAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGCGATGGGTCTTTTCTTGGATTCGTTTAAATATGAAGTCAATGACATTTTCGGATTCAGTAACCCCTTCAGTTTTGTACTAGATGCATTTTCACTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

235

Amino Acids

25.97

Weight (kDa)

9.74

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 153 - 211 6e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 299
AciI CCGC 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 655
AfaI GTAC 3 cut(s) 163, 530, 681
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 4 cut(s) 235, 347, 432, 553
AhlI ACTAGT 1 cut(s) 158
AluBI AGCT 4 cut(s) 88, 178, 341, 596
AluI AGCT 4 cut(s) 88, 178, 341, 596
Alw26I GTCTC 1 cut(s) 459
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 2 cut(s) 486, 519
ApaI GGGCCC 1 cut(s) 523
ApeKI GCWGC 1 cut(s) 189
AseI ATTAAT 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 519, 520
BaeGI GKGCMC 2 cut(s) 174, 523
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 176
BccI CCATC 2 cut(s) 295, 601
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 4 cut(s) 159, 221, 413, 683
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 2 cut(s) 190, 486
BlsI GCNGC 2 cut(s) 191, 487
BmcAI AGTACT 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 519, 520
BmiI GGNNCC 4 cut(s) 112, 361, 520, 521
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 676
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 466, 473
Bse3DI GCAATG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 2 cut(s) 466, 473
BseSI GKGCMC 2 cut(s) 174, 523
BseXI GCAGC 1 cut(s) 176
BseYI CCCAGC 2 cut(s) 127, 596
BshFI GGCC 2 cut(s) 488, 521
BshNI GGYRCC 1 cut(s) 359
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 2 cut(s) 488, 521
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 2 cut(s) 174, 523
Bsp1407I TGTACA 1 cut(s) 528
BspACI CCGC 1 cut(s) 485
BspANI GGCC 2 cut(s) 488, 521
BspCNI CTCAG 1 cut(s) 36
BspLI GGNNCC 4 cut(s) 112, 361, 520, 521
BspT107I GGYRCC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 2 cut(s) 466, 473
BssECI CCNNGG 1 cut(s) 489
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 4 cut(s) 30, 61, 275, 448
Bst6I CTCTTC 2 cut(s) 222, 546
BstAUI TGTACA 1 cut(s) 528
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 2 cut(s) 23, 99
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 459
BstMWI GCNNNNNNNGC 3 cut(s) 309, 491, 602
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 2 cut(s) 174, 523
BstV1I GCAGC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 563
BsuRI GGCC 2 cut(s) 488, 521
BtgZI GCGATG 1 cut(s) 620
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 3 cut(s) 66, 268, 280
Cac8I GCNNGC 1 cut(s) 603
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 519, 520
Csp6I GTAC 3 cut(s) 162, 529, 680
CviAII CATG 2 cut(s) 246, 526
CviQI GTAC 3 cut(s) 162, 529, 680
DdeI CTNAG 2 cut(s) 23, 99
DraI TTTAAA 1 cut(s) 631
Eam1104I CTCTTC 2 cut(s) 222, 546
EarI CTCTTC 2 cut(s) 222, 546
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 523
Eco47III AGCGCT 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 655
EcoO109I RGGNCCY 2 cut(s) 519, 520
EcoT14I CCWWGG 1 cut(s) 489
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 2 cut(s) 249, 529
FaiI YATR 8 cut(s) 36, 38, 122, 206, 247, 441, 527, 636
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 485
FatI CATG 2 cut(s) 245, 525
Fnu4HI GCNGC 2 cut(s) 190, 486
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 2 cut(s) 190, 486
FspBI CTAG 4 cut(s) 159, 221, 413, 683
GlaI GCGC 1 cut(s) 126
GluI GCNGC 2 cut(s) 190, 486
GsaI CCCAGC 2 cut(s) 131, 600
GsuI CTGGAG 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 488, 521
HhaI GCGC 1 cut(s) 127
Hin1II CATG 2 cut(s) 249, 529
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 2 cut(s) 623, 657
Hpy166II GTNNAC 2 cut(s) 226, 389
Hpy188I TCNGA 3 cut(s) 26, 257, 656
Hpy188III TCNNGA 2 cut(s) 114, 235
Hpy8I GTNNAC 2 cut(s) 226, 389
HpyAV CCTTC 1 cut(s) 679
HpyCH4III ACNGT 4 cut(s) 30, 61, 275, 448
HpyCH4V TGCA 3 cut(s) 53, 140, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 491, 602
HpyF3I CTNAG 2 cut(s) 23, 99
Hsp92II CATG 2 cut(s) 249, 529
HspAI GCGC 1 cut(s) 125
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 1 cut(s) 676
MaeI CTAG 4 cut(s) 159, 221, 413, 683
MaeIII GTNAC 1 cut(s) 662
MboII GAAGA 7 cut(s) 87, 190, 209, 400, 533, 565, 568
MhlI GDGCHC 2 cut(s) 174, 523
MluCI AATT 6 cut(s) 54, 181, 229, 324, 366, 583
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 6 cut(s) 32, 225, 231, 377, 502, 533
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 5 cut(s) 84, 323, 582, 630, 706
MwoI GCNNNNNNNGC 3 cut(s) 309, 491, 602
NlaIII CATG 2 cut(s) 249, 529
NlaIV GGNNCC 4 cut(s) 112, 361, 520, 521
NsiI ATGCAT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 623, 657
PflMI CCANNNNNTGG 1 cut(s) 299
PkrI GCNGC 2 cut(s) 191, 487
PshBI ATTAAT 1 cut(s) 323
PspFI CCCAGC 2 cut(s) 127, 596
PspN4I GGNNCC 4 cut(s) 112, 361, 520, 521
PspOMI GGGCCC 1 cut(s) 519
PspPI GGNCC 2 cut(s) 519, 520
RsaI GTAC 3 cut(s) 163, 530, 681
RsaNI GTAC 3 cut(s) 162, 529, 680
SaqAI TTAA 5 cut(s) 84, 323, 582, 630, 706
SatI GCNGC 2 cut(s) 190, 486
Sau96I GGNCC 2 cut(s) 519, 520
ScaI AGTACT 1 cut(s) 163
SduI GDGCHC 2 cut(s) 174, 523
SetI ASST 8 cut(s) 71, 90, 180, 217, 343, 361, 388, 598
SfaNI GCATC 1 cut(s) 676
SfcI CTRYAG 1 cut(s) 306
SpeI ACTAGT 1 cut(s) 158
Sse9I AATT 6 cut(s) 54, 181, 229, 324, 366, 583
SsiI CCGC 1 cut(s) 485
SspMI CTAG 4 cut(s) 159, 221, 413, 683
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 30, 61, 275, 448
TasI AATT 6 cut(s) 54, 181, 229, 324, 366, 583
TatI WGTACW 3 cut(s) 161, 528, 679
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 623, 657
Tru1I TTAA 5 cut(s) 84, 323, 582, 630, 706
Tru9I TTAA 5 cut(s) 84, 323, 582, 630, 706
TscAI CASTG 3 cut(s) 66, 268, 280
TseI GCWGC 1 cut(s) 189
TspDTI ATGAA 2 cut(s) 234, 651
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 66, 268, 280
Van91I CCANNNNNTGG 1 cut(s) 299
VspI ATTAAT 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 4 cut(s) 159, 221, 413, 683
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.