RchiOBHm_Chr1g0356851

E3 ubiquitin-protein ligase RHA1B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005870
Physical Location & Seq
Reverse (-)
49235410 .. 49236792
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
ATGGGCTTCCCAGTGGGTTACACAGAGGTCTTCTTCCCCAACCTCTTCCTCCACACCCTCTCTCTTTTGGGTTTCATCCGCAACCTCATTTTCTTGCTCTTCCACTTTCTGGGCCTTTCCGATTTCCTTGAGACTGACGCGGGCGTCTGGCCTGAGACCCGGGCCCAGCTGCTGCCCGGGTGCCAGACGCCCGCGTCGGTCCTTCTGATCCGGGAATTTCTCCCTGTGATCAGGTTTGAGGACATCGCCGGAGACCCACCGGAGAGCTGCGCCGTCTGCCTGTACGAGTTCGAGGGGAGTGAGGAGATCAGGTGCTTGACGAATTGCAAGCACATTTTTCACCGAGCGTGTTTGGACCGTTGGATGGACCACGACCAGAAGACGTGTCCGCTTTGCCGGACCGCGTTTGTGCCTGAAGAGGCCAGCGAGGAGTTGAATCGACGGCTCTGGGCCACCGCCGAGTCGGAGCTGGAGCTGGAGCTCAACCGTGATTTTTACAGTGAGTACAGTTCGGTCTGA

Protein Analysis

172

Amino Acids

19.85

Weight (kDa)

4.64

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 89 - 133 2e-12 Ring finger domain
zf-RING_11 PF17123 90 - 118 6.7e-08 RING-like zinc finger
zf-RING_5 PF14634 90 - 134 1.1e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 90 - 132 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 90 - 132 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 90 - 130 3.1e-06 RING-type zinc-finger
zf-rbx1 PF12678 97 - 133 1.5e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 143, 193
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 109
AccII CGCG 3 cut(s) 140, 194, 404
AciI CCGC 6 cut(s) 79, 140, 192, 389, 402, 456
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 2 cut(s) 144, 188
AfaI GTAC 2 cut(s) 284, 506
AfiI CCNNNNNNNGG 2 cut(s) 109, 364
AflIII ACRYGT 1 cut(s) 383
AgsI TTSAA 1 cut(s) 436
AjiI CACGTC 1 cut(s) 384
AluBI AGCT 5 cut(s) 169, 267, 469, 475, 481
AluI AGCT 5 cut(s) 169, 267, 469, 475, 481
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 3 cut(s) 125, 149, 246
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 2 cut(s) 159, 176
AoxI GGCC 5 cut(s) 112, 149, 162, 420, 450
ApaI GGGCCC 1 cut(s) 166
ApeKI GCWGC 3 cut(s) 169, 172, 267
ApoI RAATTY 1 cut(s) 215
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 8 cut(s) 112, 162, 163, 199, 355, 367, 399, 450
AsuC2I CCSGG 5 cut(s) 160, 161, 177, 178, 212
AsuHPI GGTGA 1 cut(s) 332
AvaI CYCGRG 2 cut(s) 159, 176
AvaII GGWCC 4 cut(s) 199, 355, 367, 399
BaeGI GKGCMC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 2 cut(s) 166, 483
BbsI GAAGAC 2 cut(s) 22, 386
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 156, 159, 254
BccI CCATC 1 cut(s) 358
BceAI ACGGC 2 cut(s) 257, 458
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 5 cut(s) 160, 161, 177, 178, 212
BcoDI GTCTC 3 cut(s) 125, 149, 246
BisI GCNGC 3 cut(s) 170, 173, 268
BlsI GCNGC 3 cut(s) 171, 174, 269
Bme1390I CCNGG 5 cut(s) 160, 161, 177, 178, 212
Bme18I GGWCC 4 cut(s) 199, 355, 367, 399
BmeT110I CYCGRG 2 cut(s) 159, 176
BmgBI CACGTC 1 cut(s) 384
BmgT120I GGNCC 8 cut(s) 112, 162, 163, 199, 355, 367, 399, 450
BmiI GGNNCC 2 cut(s) 164, 182
BmrFI CCNGG 5 cut(s) 160, 161, 177, 178, 212
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 2 cut(s) 22, 386
BpmI CTGGAG 2 cut(s) 491, 497
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 5 cut(s) 160, 161, 177, 178, 212
BsaHI GRCGYC 2 cut(s) 144, 188
BsaI GGTCTC 2 cut(s) 149, 246
BsaJI CCNNGG 2 cut(s) 159, 176
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 364
Bse1I ACTGG 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 159, 176
BseGI GGATG 2 cut(s) 75, 369
BseLI CCNNNNNNNGG 2 cut(s) 109, 364
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 317, 443
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 3 cut(s) 156, 159, 254
BseYI CCCAGC 1 cut(s) 165
Bsh1236I CGCG 3 cut(s) 140, 194, 404
BshFI GGCC 5 cut(s) 114, 151, 164, 422, 452
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 2 cut(s) 159, 176
BsiSI CCGG 6 cut(s) 160, 177, 211, 249, 260, 397
BslI CCNNNNNNNGG 2 cut(s) 109, 364
BsmAI GTCTC 3 cut(s) 125, 149, 246
BsnI GGCC 5 cut(s) 114, 151, 164, 422, 452
Bso31I GGTCTC 2 cut(s) 149, 246
BsoBI CYCGRG 2 cut(s) 159, 176
Bsp120I GGGCCC 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 166, 483
Bsp143I GATC 3 cut(s) 207, 228, 306
BspACI CCGC 6 cut(s) 79, 140, 192, 389, 402, 456
BspANI GGCC 5 cut(s) 114, 151, 164, 422, 452
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 3 cut(s) 140, 194, 404
BspLI GGNNCC 2 cut(s) 164, 182
BspPI GGATC 1 cut(s) 202
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 2 cut(s) 149, 246
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 159, 176
BssMI GATC 3 cut(s) 207, 228, 306
BssNI GRCGYC 2 cut(s) 144, 188
Bst4CI ACNGT 4 cut(s) 359, 488, 500, 509
Bst6I CTCTTC 3 cut(s) 50, 104, 411
BstACI GRCGYC 2 cut(s) 144, 188
BstC8I GCNNGC 4 cut(s) 142, 192, 329, 424
BstDEI CTNAG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 75, 369
BstFNI CGCG 3 cut(s) 140, 194, 404
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 3 cut(s) 210, 231, 309
BstMAI GTCTC 3 cut(s) 125, 149, 246
BstMBI GATC 3 cut(s) 207, 228, 306
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstSCI CCNGG 5 cut(s) 158, 159, 175, 176, 210
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 3 cut(s) 140, 194, 404
BstV1I GCAGC 3 cut(s) 156, 159, 254
BstV2I GAAGAC 2 cut(s) 22, 386
BsuRI GGCC 5 cut(s) 114, 151, 164, 422, 452
BtgZI GCGATG 1 cut(s) 229
BtrI CACGTC 1 cut(s) 384
BtsCI GGATG 2 cut(s) 75, 369
BtsIMutI CAGTG 2 cut(s) 18, 505
Cac8I GCNNGC 4 cut(s) 142, 192, 329, 424
CaiI CAGNNNCTG 1 cut(s) 172
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 8 cut(s) 112, 162, 163, 199, 355, 367, 399, 450
Cfr9I CCCGGG 2 cut(s) 159, 176
CpoI CGGWCCG 1 cut(s) 399
CseI GACGC 4 cut(s) 133, 146, 183, 196
Csp6I GTAC 2 cut(s) 283, 505
CspI CGGWCCG 1 cut(s) 399
CviQI GTAC 2 cut(s) 283, 505
DdeI CTNAG 1 cut(s) 153
DpnI GATC 3 cut(s) 209, 230, 308
DpnII GATC 3 cut(s) 207, 228, 306
DrdI GACNNNNNNGTC 2 cut(s) 143, 193
DseDI GACNNNNNNGTC 2 cut(s) 143, 193
Eam1104I CTCTTC 3 cut(s) 50, 104, 411
EarI CTCTTC 3 cut(s) 50, 104, 411
Ecl136II GAGCTC 1 cut(s) 481
Eco24I GRGCYC 2 cut(s) 166, 483
Eco31I GGTCTC 2 cut(s) 149, 246
Eco47I GGWCC 4 cut(s) 199, 355, 367, 399
Eco53kI GAGCTC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 2 cut(s) 159, 176
EcoICRI GAGCTC 1 cut(s) 481
EcoT38I GRGCYC 2 cut(s) 166, 483
FauI CCCGC 2 cut(s) 133, 199
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 170, 173, 268
FokI GGATG 2 cut(s) 62, 376
FriOI GRGCYC 2 cut(s) 166, 483
Fsp4HI GCNGC 3 cut(s) 170, 173, 268
GlaI GCGC 1 cut(s) 271
GluI GCNGC 3 cut(s) 170, 173, 268
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 2 cut(s) 491, 497
HaeIII GGCC 5 cut(s) 114, 151, 164, 422, 452
HapII CCGG 6 cut(s) 160, 177, 211, 249, 260, 397
HgaI GACGC 4 cut(s) 133, 146, 183, 196
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 2 cut(s) 144, 188
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 2 cut(s) 436, 461
HpaII CCGG 6 cut(s) 160, 177, 211, 249, 260, 397
HphI GGTGA 1 cut(s) 332
Hpy188I TCNGA 4 cut(s) 121, 207, 466, 518
Hpy99I CGWCG 2 cut(s) 199, 444
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 4 cut(s) 359, 488, 500, 509
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 2 cut(s) 144, 188
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 3 cut(s) 207, 228, 306
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 3 cut(s) 466, 472, 478
Lsp1109I GCAGC 3 cut(s) 156, 159, 254
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 17
MalI GATC 3 cut(s) 209, 230, 308
MboI GATC 3 cut(s) 207, 228, 306
MboII GAAGA 6 cut(s) 22, 25, 37, 91, 391, 428
MhlI GDGCHC 2 cut(s) 166, 483
MluCI AATT 2 cut(s) 215, 322
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 2 cut(s) 341, 444
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 6 cut(s) 160, 177, 211, 249, 260, 397
MspR9I CCNGG 5 cut(s) 160, 161, 177, 178, 212
MvnI CGCG 3 cut(s) 140, 194, 404
MwoI GCNNNNNNNGC 1 cut(s) 276
NciI CCSGG 5 cut(s) 160, 161, 177, 178, 212
NdeII GATC 3 cut(s) 207, 228, 306
NlaIV GGNNCC 2 cut(s) 164, 182
NmeAIII GCCGAG 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 3 cut(s) 171, 174, 269
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 483
PspFI CCCAGC 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 164, 182
PspOMI GGGCCC 1 cut(s) 162
PspPI GGNCC 8 cut(s) 112, 162, 163, 199, 355, 367, 399, 450
PstNI CAGNNNCTG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 2 cut(s) 284, 506
RsaNI GTAC 2 cut(s) 283, 505
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 483
SapI GCTCTTC 1 cut(s) 104
SatI GCNGC 3 cut(s) 170, 173, 268
Sau3AI GATC 3 cut(s) 207, 228, 306
Sau96I GGNCC 8 cut(s) 112, 162, 163, 199, 355, 367, 399, 450
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 5 cut(s) 160, 161, 177, 178, 212
SduI GDGCHC 2 cut(s) 166, 483
SinI GGWCC 4 cut(s) 199, 355, 367, 399
SmaI CCCGGG 2 cut(s) 161, 178
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 2 cut(s) 215, 322
SsiI CCGC 6 cut(s) 79, 140, 192, 389, 402, 456
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 5 cut(s) 158, 159, 175, 176, 210
TaaI ACNGT 4 cut(s) 359, 488, 500, 509
TaiI ACGT 1 cut(s) 386
TaqI TCGA 2 cut(s) 291, 439
TaqII GACCGA 2 cut(s) 187, 502
TasI AATT 2 cut(s) 215, 322
TatI WGTACW 1 cut(s) 504
TfiI GAWTC 1 cut(s) 436
TscAI CASTG 2 cut(s) 18, 505
TseI GCWGC 3 cut(s) 169, 172, 267
TspDTI ATGAA 1 cut(s) 64
TspMI CCCGGG 2 cut(s) 159, 176
TspRI CASTG 2 cut(s) 18, 505
Van91I CCANNNNNTGG 1 cut(s) 109
VpaK11BI GGWCC 4 cut(s) 199, 355, 367, 399
XapI RAATTY 1 cut(s) 215
XmaI CCCGGG 2 cut(s) 159, 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.