RchiOBHm_Chr1g0370131

Protein EARLY FLOWERING 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007141
Physical Location & Seq
Forward (+)
59689304 .. 59691173
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGAACCAAGCTCAAACCCACGATATTGCTAACTGTGATTTAAGCATTAAAAAAAAAAAAGTGAATGATGATTTTGTTGCCACCGACCGAACCCAAAGCAAAGCAGACAAAGAAACCTACCTACCAAACCGACCAAAAAATTTCCATGCAGCTCACATGACCACCCGCGAATCCTCCTCCGGACTCGACTCTTCGAGCATGGACGATCAGCCCACCAAACGCACCGTTTCACTTGATCCGGAACCCGAACCCGGCGGCGAGGTCTGGGTCGAAAACTTCAACCGCAGCTTCCGCCAGGTGCAGGCCGTCCTCGACCGCAACCGCGCTCTGATCCAGCAAGTCAACGACAACCACCAGTCCCGGATTCCGGCCAACATGGTCAAGAACGTCGCCCTCATCCAGGAAATCAACGCCAACATCCGCAAGGTCGTCTCGCTCTACTCCGACCTGTCCTCCGATTTCTCCACCGTCTTTCCCAACAAGGAGCGCTGCGATCCCAACTAG

Protein Analysis

167

Amino Acids

18.92

Weight (kDa)

6.42

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elf4 PF07011 88 - 163 1.2e-36 Early Flowering 4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 168, 324
AccIII TCCGGA 2 cut(s) 179, 238
AciI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
AclWI GGATC 3 cut(s) 230, 325, 488
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 139
AfeI AGCGCT 1 cut(s) 488
AfiI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 2 cut(s) 294, 399
AluBI AGCT 3 cut(s) 11, 152, 288
AluI AGCT 3 cut(s) 11, 152, 288
Alw26I GTCTC 1 cut(s) 436
AlwI GGATC 3 cut(s) 230, 325, 488
Aor13HI TCCGGA 2 cut(s) 179, 238
Aor51HI AGCGCT 1 cut(s) 488
AoxI GGCC 2 cut(s) 303, 369
ApeKI GCWGC 3 cut(s) 149, 285, 489
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 326, 489
AsuC2I CCSGG 2 cut(s) 252, 361
BbvI GCAGC 3 cut(s) 161, 297, 476
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 2 cut(s) 296, 401
BcnI CCSGG 2 cut(s) 252, 361
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 1 cut(s) 502
BfoI RGCGCY 1 cut(s) 490
BisI GCNGC 4 cut(s) 150, 256, 286, 490
BlsI GCNGC 4 cut(s) 151, 257, 287, 491
Bme1390I CCNGG 4 cut(s) 252, 296, 361, 401
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 4 cut(s) 252, 296, 361, 401
BpuMI CCSGG 2 cut(s) 252, 361
BsaWI WCCGGW 2 cut(s) 179, 238
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
Bse1I ACTGG 1 cut(s) 355
BseAI TCCGGA 2 cut(s) 179, 238
BseBI CCWGG 2 cut(s) 296, 401
BseGI GGATG 2 cut(s) 396, 417
BseLI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 161, 297, 476
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 2 cut(s) 168, 324
Bsh1285I CGRYCG 2 cut(s) 88, 316
BshFI GGCC 2 cut(s) 305, 371
BsiEI CGRYCG 2 cut(s) 88, 316
BsiSI CCGG 5 cut(s) 180, 239, 252, 361, 368
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
BsmAI GTCTC 1 cut(s) 436
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 305, 371
Bsp13I TCCGGA 2 cut(s) 179, 238
Bsp143I GATC 4 cut(s) 205, 235, 330, 493
BspACI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
BspANI GGCC 2 cut(s) 305, 371
BspEI TCCGGA 2 cut(s) 179, 238
BspFNI CGCG 2 cut(s) 168, 324
BspLI GGNNCC 1 cut(s) 243
BspPI GGATC 3 cut(s) 230, 325, 488
BsrI ACTGG 1 cut(s) 355
BssMI GATC 4 cut(s) 205, 235, 330, 493
Bst2UI CCWGG 2 cut(s) 296, 401
Bst4CI ACNGT 3 cut(s) 35, 226, 469
Bst6I CTCTTC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 303
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 396, 417
BstFNI CGCG 2 cut(s) 168, 324
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 2 cut(s) 326, 489
BstKTI GATC 4 cut(s) 208, 238, 333, 496
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 4 cut(s) 205, 235, 330, 493
BstMCI CGRYCG 2 cut(s) 88, 316
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 2 cut(s) 296, 401
BstSCI CCNGG 4 cut(s) 250, 294, 359, 399
BstUI CGCG 2 cut(s) 168, 324
BstV1I GCAGC 3 cut(s) 161, 297, 476
BsuRI GGCC 2 cut(s) 305, 371
BtsCI GGATG 2 cut(s) 396, 417
Cac8I GCNNGC 1 cut(s) 303
CfoI GCGC 2 cut(s) 326, 489
CviAII CATG 4 cut(s) 146, 157, 199, 376
CviJI RGCY 6 cut(s) 11, 152, 211, 288, 305, 371
CviKI_1 RGCY 6 cut(s) 11, 152, 211, 288, 305, 371
DpnI GATC 4 cut(s) 207, 237, 332, 495
DpnII GATC 4 cut(s) 205, 235, 330, 493
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 281
Eco47III AGCGCT 1 cut(s) 488
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 294, 399
Esp3I CGTCTC 1 cut(s) 436
FaeI CATG 4 cut(s) 149, 160, 202, 379
FaiI YATR 4 cut(s) 147, 158, 200, 377
FaqI GGGAC 1 cut(s) 343
FatI CATG 4 cut(s) 145, 156, 198, 375
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 4 cut(s) 150, 256, 286, 490
FokI GGATG 2 cut(s) 383, 404
Fsp4HI GCNGC 4 cut(s) 150, 256, 286, 490
FspBI CTAG 1 cut(s) 502
GlaI GCGC 2 cut(s) 325, 488
GluI GCNGC 4 cut(s) 150, 256, 286, 490
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 2 cut(s) 305, 371
HapII CCGG 5 cut(s) 180, 239, 252, 361, 368
HhaI GCGC 2 cut(s) 326, 489
Hin1II CATG 4 cut(s) 149, 160, 202, 379
Hin6I GCGC 2 cut(s) 324, 487
HinP1I GCGC 2 cut(s) 324, 487
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 4 cut(s) 170, 183, 188, 364
HpaII CCGG 5 cut(s) 180, 239, 252, 361, 368
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 330, 445, 457
Hpy188III TCNNGA 3 cut(s) 180, 239, 382
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 3 cut(s) 35, 226, 469
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 2 cut(s) 149, 301
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 4 cut(s) 149, 160, 202, 379
HspAI GCGC 2 cut(s) 324, 487
Kpn2I TCCGGA 2 cut(s) 179, 238
Kzo9I GATC 4 cut(s) 205, 235, 330, 493
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 3 cut(s) 161, 297, 476
MaeI CTAG 1 cut(s) 502
MaeII ACGT 1 cut(s) 387
MalI GATC 4 cut(s) 207, 237, 332, 495
MboI GATC 4 cut(s) 205, 235, 330, 493
MboII GAAGA 1 cut(s) 183
MluCI AATT 1 cut(s) 139
MlyI GAGTC 2 cut(s) 177, 182
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 6 cut(s) 184, 187, 253, 320, 404, 463
MroI TCCGGA 2 cut(s) 179, 238
MseI TTAA 2 cut(s) 41, 48
MspI CCGG 5 cut(s) 180, 239, 252, 361, 368
MspR9I CCNGG 4 cut(s) 252, 296, 361, 401
MvaI CCWGG 2 cut(s) 296, 401
MvnI CGCG 2 cut(s) 168, 324
MwoI GCNNNNNNNGC 1 cut(s) 291
NciI CCSGG 2 cut(s) 252, 361
NdeII GATC 4 cut(s) 205, 235, 330, 493
NlaIII CATG 4 cut(s) 149, 160, 202, 379
NlaIV GGNNCC 1 cut(s) 243
PfeI GAWTC 2 cut(s) 170, 364
PfoI TCCNGGA 2 cut(s) 359, 399
PkrI GCNGC 4 cut(s) 151, 257, 287, 491
PleI GAGTC 2 cut(s) 177, 182
PpsI GAGTC 2 cut(s) 177, 182
Psp6I CCWGG 2 cut(s) 294, 399
PspGI CCWGG 2 cut(s) 294, 399
PspN4I GGNNCC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 41, 48
SatI GCNGC 4 cut(s) 150, 256, 286, 490
Sau3AI GATC 4 cut(s) 205, 235, 330, 493
SchI GAGTC 2 cut(s) 177, 182
ScrFI CCNGG 4 cut(s) 252, 296, 361, 401
Sse9I AATT 1 cut(s) 139
SsiI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
SspMI CTAG 1 cut(s) 502
StyD4I CCNGG 4 cut(s) 250, 294, 359, 399
TaaI ACNGT 3 cut(s) 35, 226, 469
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 186, 194, 270, 312
TaqII GACCGA 1 cut(s) 102
TasI AATT 1 cut(s) 139
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 2 cut(s) 170, 364
Tru1I TTAA 2 cut(s) 41, 48
Tru9I TTAA 2 cut(s) 41, 48
TseI GCWGC 3 cut(s) 149, 285, 489
TspDTI ATGAA 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 1 cut(s) 139
XspI CTAG 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.