RchiOBHm_Chr1g0370131

Protein EARLY FLOWERING 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007141
Physical Location & Seq
Forward (+)
59689304 .. 59691173
1870 bp
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UTR
Exon/CDS
Intron
PRQ59433

Sequence Viewer

Length: 504 bp
ATGAACCAAGCTCAAACCCACGATATTGCTAACTGTGATTTAAGCATTAAAAAAAAAAAAGTGAATGATGATTTTGTTGCCACCGACCGAACCCAAAGCAAAGCAGACAAAGAAACCTACCTACCAAACCGACCAAAAAATTTCCATGCAGCTCACATGACCACCCGCGAATCCTCCTCCGGACTCGACTCTTCGAGCATGGACGATCAGCCCACCAAACGCACCGTTTCACTTGATCCGGAACCCGAACCCGGCGGCGAGGTCTGGGTCGAAAACTTCAACCGCAGCTTCCGCCAGGTGCAGGCCGTCCTCGACCGCAACCGCGCTCTGATCCAGCAAGTCAACGACAACCACCAGTCCCGGATTCCGGCCAACATGGTCAAGAACGTCGCCCTCATCCAGGAAATCAACGCCAACATCCGCAAGGTCGTCTCGCTCTACTCCGACCTGTCCTCCGATTTCTCCACCGTCTTTCCCAACAAGGAGCGCTGCGATCCCAACTAG

Protein Analysis

167

Amino Acids

18.92

Weight (kDa)

6.42

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elf4 PF07011 88 - 163 1.2e-36 Early Flowering 4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 168, 324
AccIII TCCGGA 2 cut(s) 179, 238
AciI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
AclWI GGATC 3 cut(s) 230, 325, 488
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 139
AfeI AGCGCT 1 cut(s) 488
AfiI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 2 cut(s) 294, 399
AluBI AGCT 3 cut(s) 11, 152, 288
AluI AGCT 3 cut(s) 11, 152, 288
Alw26I GTCTC 1 cut(s) 436
AlwI GGATC 3 cut(s) 230, 325, 488
Aor13HI TCCGGA 2 cut(s) 179, 238
Aor51HI AGCGCT 1 cut(s) 488
AoxI GGCC 2 cut(s) 303, 369
ApeKI GCWGC 3 cut(s) 149, 285, 489
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 326, 489
AsuC2I CCSGG 2 cut(s) 252, 361
BbvI GCAGC 3 cut(s) 161, 297, 476
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 2 cut(s) 296, 401
BcnI CCSGG 2 cut(s) 252, 361
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 1 cut(s) 502
BfoI RGCGCY 1 cut(s) 490
BisI GCNGC 4 cut(s) 150, 256, 286, 490
BlsI GCNGC 4 cut(s) 151, 257, 287, 491
Bme1390I CCNGG 4 cut(s) 252, 296, 361, 401
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 4 cut(s) 252, 296, 361, 401
BpuMI CCSGG 2 cut(s) 252, 361
BsaWI WCCGGW 2 cut(s) 179, 238
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
Bse1I ACTGG 1 cut(s) 355
BseAI TCCGGA 2 cut(s) 179, 238
BseBI CCWGG 2 cut(s) 296, 401
BseGI GGATG 2 cut(s) 396, 417
BseLI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 161, 297, 476
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 2 cut(s) 168, 324
Bsh1285I CGRYCG 2 cut(s) 88, 316
BshFI GGCC 2 cut(s) 305, 371
BsiEI CGRYCG 2 cut(s) 88, 316
BsiSI CCGG 5 cut(s) 180, 239, 252, 361, 368
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 4 cut(s) 251, 301, 367, 400
BsmAI GTCTC 1 cut(s) 436
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 305, 371
Bsp13I TCCGGA 2 cut(s) 179, 238
Bsp143I GATC 4 cut(s) 205, 235, 330, 493
BspACI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
BspANI GGCC 2 cut(s) 305, 371
BspEI TCCGGA 2 cut(s) 179, 238
BspFNI CGCG 2 cut(s) 168, 324
BspLI GGNNCC 1 cut(s) 243
BspPI GGATC 3 cut(s) 230, 325, 488
BsrI ACTGG 1 cut(s) 355
BssMI GATC 4 cut(s) 205, 235, 330, 493
Bst2UI CCWGG 2 cut(s) 296, 401
Bst4CI ACNGT 3 cut(s) 35, 226, 469
Bst6I CTCTTC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 303
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 396, 417
BstFNI CGCG 2 cut(s) 168, 324
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 2 cut(s) 326, 489
BstKTI GATC 4 cut(s) 208, 238, 333, 496
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 4 cut(s) 205, 235, 330, 493
BstMCI CGRYCG 2 cut(s) 88, 316
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 2 cut(s) 296, 401
BstSCI CCNGG 4 cut(s) 250, 294, 359, 399
BstUI CGCG 2 cut(s) 168, 324
BstV1I GCAGC 3 cut(s) 161, 297, 476
BsuRI GGCC 2 cut(s) 305, 371
BtsCI GGATG 2 cut(s) 396, 417
Cac8I GCNNGC 1 cut(s) 303
CfoI GCGC 2 cut(s) 326, 489
CviAII CATG 4 cut(s) 146, 157, 199, 376
CviJI RGCY 6 cut(s) 11, 152, 211, 288, 305, 371
CviKI_1 RGCY 6 cut(s) 11, 152, 211, 288, 305, 371
DpnI GATC 4 cut(s) 207, 237, 332, 495
DpnII GATC 4 cut(s) 205, 235, 330, 493
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 281
Eco47III AGCGCT 1 cut(s) 488
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 294, 399
Esp3I CGTCTC 1 cut(s) 436
FaeI CATG 4 cut(s) 149, 160, 202, 379
FaiI YATR 4 cut(s) 147, 158, 200, 377
FaqI GGGAC 1 cut(s) 343
FatI CATG 4 cut(s) 145, 156, 198, 375
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 4 cut(s) 150, 256, 286, 490
FokI GGATG 2 cut(s) 383, 404
Fsp4HI GCNGC 4 cut(s) 150, 256, 286, 490
FspBI CTAG 1 cut(s) 502
GlaI GCGC 2 cut(s) 325, 488
GluI GCNGC 4 cut(s) 150, 256, 286, 490
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 2 cut(s) 305, 371
HapII CCGG 5 cut(s) 180, 239, 252, 361, 368
HhaI GCGC 2 cut(s) 326, 489
Hin1II CATG 4 cut(s) 149, 160, 202, 379
Hin6I GCGC 2 cut(s) 324, 487
HinP1I GCGC 2 cut(s) 324, 487
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 4 cut(s) 170, 183, 188, 364
HpaII CCGG 5 cut(s) 180, 239, 252, 361, 368
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 330, 445, 457
Hpy188III TCNNGA 3 cut(s) 180, 239, 382
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 3 cut(s) 35, 226, 469
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 2 cut(s) 149, 301
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 4 cut(s) 149, 160, 202, 379
HspAI GCGC 2 cut(s) 324, 487
Kpn2I TCCGGA 2 cut(s) 179, 238
Kzo9I GATC 4 cut(s) 205, 235, 330, 493
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 3 cut(s) 161, 297, 476
MaeI CTAG 1 cut(s) 502
MaeII ACGT 1 cut(s) 387
MalI GATC 4 cut(s) 207, 237, 332, 495
MboI GATC 4 cut(s) 205, 235, 330, 493
MboII GAAGA 1 cut(s) 183
MluCI AATT 1 cut(s) 139
MlyI GAGTC 2 cut(s) 177, 182
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 6 cut(s) 184, 187, 253, 320, 404, 463
MroI TCCGGA 2 cut(s) 179, 238
MseI TTAA 2 cut(s) 41, 48
MspI CCGG 5 cut(s) 180, 239, 252, 361, 368
MspR9I CCNGG 4 cut(s) 252, 296, 361, 401
MvaI CCWGG 2 cut(s) 296, 401
MvnI CGCG 2 cut(s) 168, 324
MwoI GCNNNNNNNGC 1 cut(s) 291
NciI CCSGG 2 cut(s) 252, 361
NdeII GATC 4 cut(s) 205, 235, 330, 493
NlaIII CATG 4 cut(s) 149, 160, 202, 379
NlaIV GGNNCC 1 cut(s) 243
PfeI GAWTC 2 cut(s) 170, 364
PfoI TCCNGGA 2 cut(s) 359, 399
PkrI GCNGC 4 cut(s) 151, 257, 287, 491
PleI GAGTC 2 cut(s) 177, 182
PpsI GAGTC 2 cut(s) 177, 182
Psp6I CCWGG 2 cut(s) 294, 399
PspGI CCWGG 2 cut(s) 294, 399
PspN4I GGNNCC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 41, 48
SatI GCNGC 4 cut(s) 150, 256, 286, 490
Sau3AI GATC 4 cut(s) 205, 235, 330, 493
SchI GAGTC 2 cut(s) 177, 182
ScrFI CCNGG 4 cut(s) 252, 296, 361, 401
Sse9I AATT 1 cut(s) 139
SsiI CCGC 7 cut(s) 166, 255, 283, 292, 316, 322, 421
SspMI CTAG 1 cut(s) 502
StyD4I CCNGG 4 cut(s) 250, 294, 359, 399
TaaI ACNGT 3 cut(s) 35, 226, 469
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 186, 194, 270, 312
TaqII GACCGA 1 cut(s) 102
TasI AATT 1 cut(s) 139
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 2 cut(s) 170, 364
Tru1I TTAA 2 cut(s) 41, 48
Tru9I TTAA 2 cut(s) 41, 48
TseI GCWGC 3 cut(s) 149, 285, 489
TspDTI ATGAA 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 1 cut(s) 139
XspI CTAG 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.