Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005275

Physical Location & Seq

Genomic Coordinates Reverse (-)

60261070 .. 60264360

Gene Structure 3291 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1029 bp

ATGGCTTCCCATACTCCCAATCTGGAGTGCCGGATGTACGAGGCGCGGTACCCGGAGGTGGACATGGCGGTGATGATTCAGGTGAAGAACATCGCCGACATGGGCGCGTACGTCTCACTCCTCGAGTACAACAACATCGAGGGCATGATTCTGTTCTCGGAGCTCTCCCGCCGTCGGATTCGGAGCGTGAGCAGCCTCATCAAGGTCGGCCGCATCGAGCCCGTCATGGTTCTCAGGGTCGACAAGGAGAAGGGCTACATCGATTTGAGTAAGAGGAGAGTTTCCGAGGAGGATATCTCCGCCTGTGAGGAGAGGTACAACAAGAGCAAGCTCGTCCACTCCATCATGCGACATGTCGCTGAGACACTCGGTATCGATTTGGAGGAGTTGTATGTCAATATTGGCTGGCCGTTGTACCGGAAGTATGGTCATGCTTTTGAGGCTTTTAAAGTAATTGTCACTGATCCTGATACAGTTCTCAGTGCCCTTACATGTGAGGTGAAAGAGACCGGCCCTGATGGACAGGAGGTTACTAAGGTTGTCCCTGCTGTGTCAGAGGAAGTGAAAGACTCTCTGGTAAGGAACATCAGGAGAAGAATGACACCACAACCATTGAAGATCCGTGCTGATATTGAAATGAAATGTTTCCAGTTTGATGGTGTTCTTCACATTAAGGATGCCATGCGGAAAGCAGAAGCTGCGGGCAACAATGACTGTCCTGTGAAGATTAAGTTGGTTGCTCCTCCACTCTATGTTCTTACCACACAGACTCTTGATAAGGAACAAGGTATAACAGTTCTAGGCAATGCAATTGTAGCCTGCACAAAAGCAATAGAGCATCACAAGGGGAAACTATTGGTGAAGGAAGCACCAAGAGTGGTGAGTGAAAAGGAAGATAAATTGCTGCAAGAGCACATGAGTAAGCTCCGCCAGGAGAATGAGGAGGTTGGGGGTGATACAGGCAGTGAAGATGAAGAAGACACTGGAATGGGAGATGTTGACTTGGAGAACGGAGGCCAGGCATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0000278 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005850 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0010564 GO:0010948 GO:0019538 GO:0022402 GO:0031570 GO:0031571 GO:0032991 GO:0033290 GO:0033554 GO:0034641 GO:0034645 GO:0043021 GO:0043022 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043614 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0044877 GO:0045786 GO:0045930 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070993 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000045 GO:2000134

KEGG Pathways

Metabolic & Signaling

ko03013 ko04138 ko04140 ko04141 ko04210 ko04932 ko05160 ko05162 ko05164 ko05168 map03013 map04138 map04140 map04141 map04210 map04932 map05160 map05162 map05164 map05168

Pfam Domains

Protein Families

EIF_2_alpha S1

Protein Analysis

342

Amino Acids

38.44

Weight (kDa)

5.47

Isoelectric Point (pI)

51.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1	PF00575	18 - 92	5e-13	S1 RNA binding domain
EIF_2_alpha	PF07541	130 - 260	2.6e-34	Eukaryotic translation initiation factor 2 alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05470
fragaria_vesca	FvH4_6g15100 FvH4_7g25050
malus_domestica	MD01G1157300.v1.1 MD07G1226100.v1.1
prunus_persica	Prupe.2G255200_v2.0.a1
pyrus_communis	pycom01g17410 pycom07g20450
rosa_chinensis	RchiOBHm_Chr1g0370911 RchiOBHm_Chr3g0468371
rosa_laevigata	RLG00000024436 RLG00000026999
rosa_multiflora	Rmu_co7996168.1_g000001 Rmu_sc0001018.1_g000002 Rmu_sc0004353.1_g000010 Rmu_sc0014003.1_g000002
rosa_roxburghii	Rroxscaffold_4G00286130 Rroxscaffold_6G00412840
rosa_rugosa	Rorug01G0360200 Rorug03G0095000
rosa_samantha	Rh1AG370200 Rh1BG333800 Rh1CG347100 Rh1DG365000 Rh3AG142800 Rh3BG165200 Rh3DG164800
rosa_wichuraiana	Rw1G032580 Rw3G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	48
AccB1I	GGYRCC	1 cut(s)	48
AccI	GTMKAC	1 cut(s)	240
AccII	CGCG	2 cut(s)	46, 107
AciI	CCGC	8 cut(s)	46, 68, 169, 211, 300, 685, 701, 928
AclWI	GGATC	2 cut(s)	458, 613
AcoI	YGGCCR	2 cut(s)	208, 407
AfaI	GTAC	6 cut(s)	38, 50, 110, 128, 317, 416
AfiI	CCNNNNNNNGG	3 cut(s)	58, 174, 202
AflIII	ACRYGT	2 cut(s)	352, 491
AgsI	TTSAA	2 cut(s)	616, 635
AjnI	CCWGG	2 cut(s)	930, 1017
AjuI	GAANNNNNNNTTGG	2 cut(s)	716, 748
AluBI	AGCT	4 cut(s)	163, 331, 698, 925
AluI	AGCT	4 cut(s)	163, 331, 698, 925
Alw21I	GWGCWC	2 cut(s)	165, 915
Alw26I	GTCTC	3 cut(s)	118, 356, 500
AlwI	GGATC	2 cut(s)	458, 613
AlwNI	CAGNNNCTG	1 cut(s)	698
Ama87I	CYCGRG	1 cut(s)	122
AoxI	GGCC	4 cut(s)	208, 407, 511, 1015
ApeKI	GCWGC	3 cut(s)	192, 698, 904
Asp700I	GAANNNNTTC	1 cut(s)	644
Asp718I	GGTACC	1 cut(s)	48
AspLEI	GCGC	2 cut(s)	46, 107
AspS9I	GGNCC	1 cut(s)	512
AsuC2I	CCSGG	1 cut(s)	53
AsuHPI	GGTGA	6 cut(s)	82, 94, 511, 871, 892, 965
AvaI	CYCGRG	1 cut(s)	122
BaeGI	GKGCMC	1 cut(s)	487
BanI	GGYRCC	1 cut(s)	48
BanII	GRGCYC	2 cut(s)	165, 222
BbsI	GAAGAC	1 cut(s)	984
Bbv12I	GWGCWC	2 cut(s)	165, 915
BbvI	GCAGC	3 cut(s)	204, 685, 891
BccI	CCATC	3 cut(s)	350, 512, 650
BceAI	ACGGC	2 cut(s)	156, 394
BciT130I	CCWGG	2 cut(s)	932, 1019
BcnI	CCSGG	1 cut(s)	53
BcoDI	GTCTC	3 cut(s)	118, 356, 500
BfaI	CTAG	1 cut(s)	800
BisI	GCNGC	4 cut(s)	193, 211, 699, 905
BlsI	GCNGC	4 cut(s)	194, 212, 700, 906
Bme1390I	CCNGG	3 cut(s)	53, 932, 1019
BmeT110I	CYCGRG	1 cut(s)	122
BmgT120I	GGNCC	1 cut(s)	512
BmiI	GGNNCC	1 cut(s)	50
BmrFI	CCNGG	3 cut(s)	53, 932, 1019
BmsI	GCATC	3 cut(s)	222, 667, 847
BpiI	GAAGAC	1 cut(s)	984
BplI	GAGNNNNNCTC	2 cut(s)	281, 313
BpmI	CTGGAG	1 cut(s)	44
BpuMI	CCSGG	1 cut(s)	53
Bsa29I	ATCGAT	2 cut(s)	261, 375
BsaI	GGTCTC	1 cut(s)	500
BsaJI	CCNNGG	1 cut(s)	285
BsaWI	WCCGGW	1 cut(s)	417
BsaXI	ACNNNNNCTCC	2 cut(s)	374, 404
Bsc4I	CCNNNNNNNGG	3 cut(s)	58, 174, 202
Bse118I	RCCGGY	1 cut(s)	509
Bse1I	ACTGG	2 cut(s)	649, 988
Bse3DI	GCAATG	1 cut(s)	811
BseBI	CCWGG	2 cut(s)	932, 1019
BseCI	ATCGAT	2 cut(s)	261, 375
BseDI	CCNNGG	1 cut(s)	285
BseGI	GGATG	2 cut(s)	39, 682
BseLI	CCNNNNNNNGG	3 cut(s)	58, 174, 202
BseMI	GCAATG	1 cut(s)	811
BseMII	CTCAG	3 cut(s)	247, 351, 493
BseNI	ACTGG	2 cut(s)	649, 988
BseRI	GAGGAG	7 cut(s)	110, 289, 302, 323, 398, 732, 956
BseSI	GKGCMC	1 cut(s)	487
BseX3I	CGGCCG	1 cut(s)	208
BseXI	GCAGC	3 cut(s)	204, 685, 891
BsgI	GTGCAG	1 cut(s)	805
Bsh1236I	CGCG	2 cut(s)	46, 107
Bsh1285I	CGRYCG	1 cut(s)	211
BshFI	GGCC	4 cut(s)	210, 409, 513, 1017
BshNI	GGYRCC	1 cut(s)	48
BshVI	ATCGAT	2 cut(s)	261, 375
BsiEI	CGRYCG	1 cut(s)	211
BsiHKAI	GWGCWC	2 cut(s)	165, 915
BsiHKCI	CYCGRG	1 cut(s)	122
BsiSI	CCGG	4 cut(s)	31, 53, 418, 510
BsiWI	CGTACG	1 cut(s)	108
BslFI	GGGAC	1 cut(s)	527
BslI	CCNNNNNNNGG	3 cut(s)	58, 174, 202
BsmAI	GTCTC	3 cut(s)	118, 356, 500
BsmBI	CGTCTC	1 cut(s)	118
BsmFI	GGGAC	1 cut(s)	527
BsnI	GGCC	4 cut(s)	210, 409, 513, 1017
Bso31I	GGTCTC	1 cut(s)	500
BsoBI	CYCGRG	1 cut(s)	122
Bsp1286I	GDGCHC	4 cut(s)	165, 222, 487, 915
Bsp143I	GATC	2 cut(s)	463, 618
BspACI	CCGC	8 cut(s)	46, 68, 169, 211, 300, 685, 701, 928
BspANI	GGCC	4 cut(s)	210, 409, 513, 1017
BspCNI	CTCAG	3 cut(s)	246, 352, 492
BspDI	ATCGAT	2 cut(s)	261, 375
BspFNI	CGCG	2 cut(s)	46, 107
BspLI	GGNNCC	1 cut(s)	50
BspPI	GGATC	2 cut(s)	458, 613
BspT107I	GGYRCC	1 cut(s)	48
BspTNI	GGTCTC	1 cut(s)	500
BsrDI	GCAATG	1 cut(s)	811
BsrFI	RCCGGY	1 cut(s)	509
BsrI	ACTGG	2 cut(s)	649, 988
BssAI	RCCGGY	1 cut(s)	509
BssECI	CCNNGG	1 cut(s)	285
BssMI	GATC	2 cut(s)	463, 618
Bst2UI	CCWGG	2 cut(s)	932, 1019
Bst4CI	ACNGT	3 cut(s)	475, 716, 796
BstAPI	GCANNNNNTGC	1 cut(s)	698
BstC8I	GCNNGC	4 cut(s)	329, 407, 703, 820
BstDEI	CTNAG	4 cut(s)	233, 360, 479, 534
BstENI	CCTNNNNNAGG	1 cut(s)	200
BstF5I	GGATG	2 cut(s)	39, 682
BstFNI	CGCG	2 cut(s)	46, 107
BstHHI	GCGC	2 cut(s)	46, 107
BstKTI	GATC	2 cut(s)	466, 621
BstMAI	GTCTC	3 cut(s)	118, 356, 500
BstMBI	GATC	2 cut(s)	463, 618
BstMCI	CGRYCG	1 cut(s)	211
BstMWI	GCNNNNNNNGC	5 cut(s)	192, 440, 698, 815, 910
BstNI	CCWGG	2 cut(s)	932, 1019
BstNSI	RCATGY	2 cut(s)	356, 495
BstSCI	CCNGG	3 cut(s)	51, 930, 1017
BstSLI	GKGCMC	1 cut(s)	487
BstUI	CGCG	2 cut(s)	46, 107
BstV1I	GCAGC	3 cut(s)	204, 685, 891
BstV2I	GAAGAC	1 cut(s)	984
BstX2I	RGATCY	1 cut(s)	618
BstXI	CCANNNNNNTGG	1 cut(s)	656
BstYI	RGATCY	1 cut(s)	618
BstZI	CGGCCG	1 cut(s)	208
Bsu15I	ATCGAT	2 cut(s)	261, 375
BsuRI	GGCC	4 cut(s)	210, 409, 513, 1017
BsuTUI	ATCGAT	2 cut(s)	261, 375
BtgZI	GCGATG	1 cut(s)	76
BtsCI	GGATG	2 cut(s)	39, 682
BtsI	GCAGTG	1 cut(s)	970
BtsIMutI	CAGTG	4 cut(s)	459, 487, 970, 981
Cac8I	GCNNGC	4 cut(s)	329, 407, 703, 820
CaiI	CAGNNNCTG	1 cut(s)	698
CfoI	GCGC	2 cut(s)	46, 107
Cfr10I	RCCGGY	1 cut(s)	509
Cfr13I	GGNCC	1 cut(s)	512
ClaI	ATCGAT	2 cut(s)	261, 375
Csp6I	GTAC	6 cut(s)	37, 49, 109, 127, 316, 415
CspCI	CAANNNNNGTGG	2 cut(s)	594, 629
CviQI	GTAC	6 cut(s)	37, 49, 109, 127, 316, 415
DdeI	CTNAG	4 cut(s)	233, 360, 479, 534
DpnI	GATC	2 cut(s)	465, 620
DpnII	GATC	2 cut(s)	463, 618
DraI	TTTAAA	1 cut(s)	448
EaeI	YGGCCR	2 cut(s)	208, 407
EagI	CGGCCG	1 cut(s)	208
EciI	GGCGGA	2 cut(s)	289, 917
Ecl136II	GAGCTC	1 cut(s)	163
EclXI	CGGCCG	1 cut(s)	208
Eco24I	GRGCYC	2 cut(s)	165, 222
Eco31I	GGTCTC	1 cut(s)	500
Eco32I	GATATC	1 cut(s)	295
Eco52I	CGGCCG	1 cut(s)	208
Eco53kI	GAGCTC	1 cut(s)	163
Eco88I	CYCGRG	1 cut(s)	122
EcoICRI	GAGCTC	1 cut(s)	163
EcoNI	CCTNNNNNAGG	1 cut(s)	200
EcoRII	CCWGG	2 cut(s)	930, 1017
EcoRV	GATATC	1 cut(s)	295
EcoT38I	GRGCYC	2 cut(s)	165, 222
Esp3I	CGTCTC	1 cut(s)	118
FaqI	GGGAC	1 cut(s)	527
FauI	CCCGC	2 cut(s)	176, 694
FblI	GTMKAC	1 cut(s)	240
Fnu4HI	GCNGC	4 cut(s)	193, 211, 699, 905
FokI	GGATG	2 cut(s)	46, 689
FriOI	GRGCYC	2 cut(s)	165, 222
Fsp4HI	GCNGC	4 cut(s)	193, 211, 699, 905
FspBI	CTAG	1 cut(s)	800
GlaI	GCGC	2 cut(s)	45, 106
GluI	GCNGC	4 cut(s)	193, 211, 699, 905
GsuI	CTGGAG	1 cut(s)	44
HaeIII	GGCC	4 cut(s)	210, 409, 513, 1017
HapII	CCGG	4 cut(s)	31, 53, 418, 510
HhaI	GCGC	2 cut(s)	46, 107
Hin6I	GCGC	2 cut(s)	44, 105
HinP1I	GCGC	2 cut(s)	44, 105
HincII	GTYRAC	2 cut(s)	241, 1000
HindII	GTYRAC	2 cut(s)	241, 1000
HinfI	GANTC	5 cut(s)	76, 148, 178, 569, 769
HpaII	CCGG	4 cut(s)	31, 53, 418, 510
HphI	GGTGA	6 cut(s)	82, 94, 511, 871, 892, 965
Hpy166II	GTNNAC	4 cut(s)	61, 241, 337, 1000
Hpy188I	TCNGA	5 cut(s)	160, 177, 183, 286, 556
Hpy188III	TCNNGA	4 cut(s)	23, 467, 589, 773
Hpy8I	GTNNAC	4 cut(s)	61, 241, 337, 1000
Hpy99I	CGWCG	1 cut(s)	177
HpyAV	CCTTC	2 cut(s)	244, 856
HpyCH4III	ACNGT	3 cut(s)	475, 716, 796
HpyCH4IV	ACGT	1 cut(s)	111
HpyCH4V	TGCA	3 cut(s)	809, 822, 907
HpyF10VI	GCNNNNNNNGC	5 cut(s)	192, 440, 698, 815, 910
HpyF3I	CTNAG	4 cut(s)	233, 360, 479, 534
HpySE526I	ACGT	1 cut(s)	111
HspAI	GCGC	2 cut(s)	44, 105
KpnI	GGTACC	1 cut(s)	52
Kzo9I	GATC	2 cut(s)	463, 618
LmnI	GCTCC	4 cut(s)	160, 183, 745, 930
Lsp1109I	GCAGC	3 cut(s)	204, 685, 891
LweI	GCATC	3 cut(s)	222, 667, 847
MaeI	CTAG	1 cut(s)	800
MaeII	ACGT	1 cut(s)	111
MaeIII	GTNAC	2 cut(s)	457, 529
MalI	GATC	2 cut(s)	465, 620
MboI	GATC	2 cut(s)	463, 618
MboII	GAAGA	9 cut(s)	97, 606, 628, 656, 736, 905, 980, 986, 989
MfeI	CAATTG	1 cut(s)	810
MflI	RGATCY	1 cut(s)	618
MhlI	GDGCHC	4 cut(s)	165, 222, 487, 915
MluCI	AATT	3 cut(s)	453, 810, 899
MlyI	GAGTC	2 cut(s)	563, 763
MmeI	TCCRAC	1 cut(s)	155
MroXI	GAANNNNTTC	1 cut(s)	644
MseI	TTAA	3 cut(s)	447, 672, 729
MslI	CAYNNNNRTG	2 cut(s)	68, 986
MspI	CCGG	4 cut(s)	31, 53, 418, 510
MspR9I	CCNGG	3 cut(s)	53, 932, 1019
MunI	CAATTG	1 cut(s)	810
MvaI	CCWGG	2 cut(s)	932, 1019
MvnI	CGCG	2 cut(s)	46, 107
MwoI	GCNNNNNNNGC	5 cut(s)	192, 440, 698, 815, 910
NciI	CCSGG	1 cut(s)	53
NdeII	GATC	2 cut(s)	463, 618
NlaIV	GGNNCC	1 cut(s)	50
NmuCI	GTSAC	1 cut(s)	457
NspI	RCATGY	2 cut(s)	356, 495
PaeR7I	CTCGAG	1 cut(s)	122
PciI	ACATGT	2 cut(s)	352, 491
PcsI	WCGNNNNNNNCGW	1 cut(s)	213
PdmI	GAANNNNTTC	1 cut(s)	644
PfeI	GAWTC	3 cut(s)	76, 148, 178
Pfl23II	CGTACG	1 cut(s)	108
PkrI	GCNGC	4 cut(s)	194, 212, 700, 906
PleI	GAGTC	2 cut(s)	563, 763
PpsI	GAGTC	2 cut(s)	563, 763
PscI	ACATGT	2 cut(s)	352, 491
Psp124BI	GAGCTC	1 cut(s)	165
Psp6I	CCWGG	2 cut(s)	930, 1017
PspGI	CCWGG	2 cut(s)	930, 1017
PspLI	CGTACG	1 cut(s)	108
PspN4I	GGNNCC	1 cut(s)	50
PspPI	GGNCC	1 cut(s)	512
PspXI	VCTCGAGB	1 cut(s)	122
PstNI	CAGNNNCTG	1 cut(s)	698
PsuI	RGATCY	1 cut(s)	618
RsaI	GTAC	6 cut(s)	38, 50, 110, 128, 317, 416
RsaNI	GTAC	6 cut(s)	37, 49, 109, 127, 316, 415
RseI	CAYNNNNRTG	2 cut(s)	68, 986
SacI	GAGCTC	1 cut(s)	165
SalI	GTCGAC	1 cut(s)	239
SaqAI	TTAA	3 cut(s)	447, 672, 729
SatI	GCNGC	4 cut(s)	193, 211, 699, 905
Sau3AI	GATC	2 cut(s)	463, 618
Sau96I	GGNCC	1 cut(s)	512
SchI	GAGTC	2 cut(s)	563, 763
ScrFI	CCNGG	3 cut(s)	53, 932, 1019
SduI	GDGCHC	4 cut(s)	165, 222, 487, 915
SfaNI	GCATC	3 cut(s)	222, 667, 847
Sfr274I	CTCGAG	1 cut(s)	122
SlaI	CTCGAG	1 cut(s)	122
SmiMI	CAYNNNNRTG	2 cut(s)	68, 986
SmlI	CTYRAG	1 cut(s)	122
SmoI	CTYRAG	1 cut(s)	122
Sse9I	AATT	3 cut(s)	453, 810, 899
SsiI	CCGC	8 cut(s)	46, 68, 169, 211, 300, 685, 701, 928
SspI	AATATT	1 cut(s)	400
SspMI	CTAG	1 cut(s)	800
SstI	GAGCTC	1 cut(s)	165
StyD4I	CCNGG	3 cut(s)	51, 930, 1017
TaaI	ACNGT	3 cut(s)	475, 716, 796
TaiI	ACGT	1 cut(s)	114
TaqI	TCGA	6 cut(s)	123, 138, 216, 240, 261, 375
TasI	AATT	3 cut(s)	453, 810, 899
TatI	WGTACW	1 cut(s)	126
TauI	GCSGC	1 cut(s)	213
TfiI	GAWTC	3 cut(s)	76, 148, 178
Tru1I	TTAA	3 cut(s)	447, 672, 729
Tru9I	TTAA	3 cut(s)	447, 672, 729
TscAI	CASTG	4 cut(s)	466, 487, 970, 988
TseFI	GTSAC	1 cut(s)	457
TseI	GCWGC	3 cut(s)	192, 698, 904
Tsp45I	GTSAC	1 cut(s)	457
TspDTI	ATGAA	2 cut(s)	653, 987
TspGWI	ACGGA	2 cut(s)	611, 1026
TspRI	CASTG	4 cut(s)	466, 487, 970, 988
XagI	CCTNNNNNAGG	1 cut(s)	200
XceI	RCATGY	2 cut(s)	356, 495
XhoI	CTCGAG	1 cut(s)	122
XmiI	GTMKAC	1 cut(s)	240
XmnI	GAANNNNTTC	1 cut(s)	644
XspI	CTAG	1 cut(s)	800

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.