RchiOBHm_Chr1g0372771

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014972
Physical Location & Seq
Reverse (-)
61315943 .. 61317735
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1113 bp
ATGGTTCTCAGGCATAAGAATTATCTCACCAGCAAATTATATCCTGGTATGGGGAAACTCTGGGTTGAAGTTTGCTTGATATCTGCTCGAGGGCTCCGGCGTTCGTCGTCCCTATGGAAGCTTCAATGGTATGCTGTTGGGTGGACCAATCCCAATAACAAGTACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCAGTATGGAAAACCAAGTTTGCTACCTTGGTTGAGGACGCGGAATCTAAACTGAATGATTTGGCTCTGCACATTGAAGTCTATAGTAGAGAGCCTATATTCCTCAGAGAAAGGCTACAGGGGACAGCTACTATTCTCTTGAAAGAGTTTCTGGCTAAGCATAACAAGAATTCTGAGCATTCAAGGCATGGAGTTGAAGAAGTTGGGAGCTACCAATTGCGAAAAAAGAATTCCAACAAACCTCAAGGTTTTGTTGATGTTTCAATCCACATTTCTGAGGATATGGAAGGGAGAAGCTCATACACAGGAAATGAGGGAGGACCTGGGGGTCACAGCAATACTATCACCTTGGCTACTGGAGATGGATCTGCACCAGCATTACTACCATTTACTCCACAAAAGAGACCAGAACATCAATTCCAAATGAATTCCCCCTATGCAAATCCAATGCCTTCGACTACATACTATTCGAAACCATCTGCTGTTAGACCAAGTTACACACCAGCAAGCGGACCAAGCTACACGCCAGCAAGCGGACCAGGCAACCTGCCAGCAAGCGGACCAGGCTACCTGCCAGCAAGTGGACCAAGCTACCTGCCAGCAAGTGGACCAAGCTATCAGCCTTCTAGAACTCCTCCACCGCCTCCACCACCTTCCAATGTTGGATATGTACCTACTTTTCTCCCCAGGACAGATCAAATGACTGACACCTATATCAATATGCCCTCATCATCTGGAGCACCACCTCCTGGTCGTGGAACTCGGGCCGGAGGACCTGCTTTTGCAATGGGAATGGGTGCTGGAGCGCTGGCAGCCGGTGCGGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTTCCTTCAGGCTTACAAGATGCTAGTGTTACCATATCAGCTGATCCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

370

Amino Acids

39.63

Weight (kDa)

9.11

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 22 - 122 2e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 525
Acc36I ACCTGC 4 cut(s) 753, 777, 801, 982
AccB7I CCANNNNNTGG 3 cut(s) 779, 803, 947
AccII CGCG 1 cut(s) 239
AciI CCGC 6 cut(s) 239, 708, 732, 756, 839, 1019
AclWI GGATC 2 cut(s) 571, 1094
AcsI RAATTY 3 cut(s) 367, 427, 625
AcuI CTGAAG 1 cut(s) 1047
AfaI GTAC 2 cut(s) 164, 870
AfeI AGCGCT 1 cut(s) 1005
AfiI CCNNNNNNNGG 8 cut(s) 50, 707, 731, 755, 779, 803, 947, 953
AgsI TTSAA 7 cut(s) 68, 125, 275, 340, 381, 395, 462
AhdI GACNNNNNGTC 1 cut(s) 1042
AjnI CCWGG 6 cut(s) 43, 520, 736, 760, 884, 946
AloI GAACNNNNNNTCC 4 cut(s) 600, 632, 1041, 1073
AluBI AGCT 8 cut(s) 121, 326, 408, 495, 717, 789, 813, 1097
AluI AGCT 8 cut(s) 121, 326, 408, 495, 717, 789, 813, 1097
Alw21I GWGCWC 1 cut(s) 940
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 2 cut(s) 571, 1094
Ama87I CYCGRG 2 cut(s) 87, 960
Aor51HI AGCGCT 1 cut(s) 1005
AoxI GGCC 1 cut(s) 963
ApeKI GCWGC 1 cut(s) 1010
ApoI RAATTY 3 cut(s) 367, 427, 625
AspLEI GCGC 1 cut(s) 1006
AspS9I GGNCC 9 cut(s) 144, 518, 710, 734, 758, 782, 806, 963, 971
AsuHPI GGTGA 4 cut(s) 19, 535, 1033, 1043
AsuII TTCGAA 1 cut(s) 668
AvaI CYCGRG 2 cut(s) 87, 960
AvaII GGWCC 8 cut(s) 144, 518, 710, 734, 758, 782, 806, 971
BanII GRGCYC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 940
BbvI GCAGC 1 cut(s) 1022
BccI CCATC 2 cut(s) 554, 682
BciT130I CCWGG 6 cut(s) 45, 522, 738, 762, 886, 948
BcoDI GTCTC 1 cut(s) 595
BfaI CTAG 2 cut(s) 825, 1080
BfmI CTRYAG 2 cut(s) 280, 314
BfoI RGCGCY 1 cut(s) 1007
BfuAI ACCTGC 4 cut(s) 753, 777, 801, 982
BisI GCNGC 1 cut(s) 1011
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 1012
BmcAI AGTACT 1 cut(s) 164
Bme1390I CCNGG 6 cut(s) 45, 522, 738, 762, 886, 948
Bme18I GGWCC 8 cut(s) 144, 518, 710, 734, 758, 782, 806, 971
BmeRI GACNNNNNGTC 1 cut(s) 1042
BmeT110I CYCGRG 2 cut(s) 87, 960
BmgT120I GGNCC 9 cut(s) 144, 518, 710, 734, 758, 782, 806, 963, 971
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 6 cut(s) 45, 522, 738, 762, 886, 948
BmsI GCATC 3 cut(s) 169, 191, 1066
BpmI CTGGAG 3 cut(s) 576, 954, 1020
Bpu1102I GCTNAGC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 595
BsaJI CCNNGG 4 cut(s) 225, 521, 546, 884
Bsc4I CCNNNNNNNGG 8 cut(s) 50, 707, 731, 755, 779, 803, 947, 953
Bse118I RCCGGY 1 cut(s) 1013
Bse1I ACTGG 2 cut(s) 200, 559
Bse3DI GCAATG 1 cut(s) 990
BseBI CCWGG 6 cut(s) 45, 522, 738, 762, 886, 948
BseDI CCNNGG 4 cut(s) 225, 521, 546, 884
BseLI CCNNNNNNNGG 8 cut(s) 50, 707, 731, 755, 779, 803, 947, 953
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 4 cut(s) 22, 316, 363, 465
BseNI ACTGG 2 cut(s) 200, 559
BseRI GAGGAG 1 cut(s) 822
BseXI GCAGC 1 cut(s) 1022
BsgI GTGCAG 3 cut(s) 151, 251, 552
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 1 cut(s) 965
BsiHKAI GWGCWC 1 cut(s) 940
BsiHKCI CYCGRG 2 cut(s) 87, 960
BsiSI CCGG 3 cut(s) 97, 966, 1014
BslFI GGGAC 2 cut(s) 94, 334
BslI CCNNNNNNNGG 8 cut(s) 50, 707, 731, 755, 779, 803, 947, 953
BsmAI GTCTC 1 cut(s) 595
BsmFI GGGAC 2 cut(s) 94, 334
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 1 cut(s) 965
Bso31I GGTCTC 1 cut(s) 595
BsoBI CYCGRG 2 cut(s) 87, 960
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 2 cut(s) 96, 940
Bsp143I GATC 4 cut(s) 563, 892, 1023, 1099
Bsp1720I GCTNAGC 1 cut(s) 354
BspACI CCGC 6 cut(s) 239, 708, 732, 756, 839, 1019
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 4 cut(s) 21, 315, 364, 466
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 95
BspMI ACCTGC 4 cut(s) 753, 777, 801, 982
BspPI GGATC 2 cut(s) 571, 1094
BspT104I TTCGAA 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 990
BsrFI RCCGGY 1 cut(s) 1013
BsrI ACTGG 2 cut(s) 200, 559
BssAI RCCGGY 1 cut(s) 1013
BssECI CCNNGG 4 cut(s) 225, 521, 546, 884
BssMI GATC 4 cut(s) 563, 892, 1023, 1099
BssT1I CCWWGG 2 cut(s) 225, 546
Bst2UI CCWGG 6 cut(s) 45, 522, 738, 762, 886, 948
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 8 cut(s) 706, 726, 730, 750, 754, 774, 798, 1008
BstDEI CTNAG 5 cut(s) 8, 302, 354, 372, 474
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 1007
BstHHI GCGC 1 cut(s) 1006
BstKTI GATC 4 cut(s) 566, 895, 1026, 1102
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 4 cut(s) 563, 892, 1023, 1099
BstMWI GCNNNNNNNGC 6 cut(s) 382, 714, 738, 762, 1010, 1016
BstNI CCWGG 6 cut(s) 45, 522, 738, 762, 886, 948
BstSCI CCNGG 6 cut(s) 43, 520, 736, 760, 884, 946
BstSFI CTRYAG 2 cut(s) 280, 314
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 1022
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BsuRI GGCC 1 cut(s) 965
BveI ACCTGC 4 cut(s) 753, 777, 801, 982
Cac8I GCNNGC 8 cut(s) 706, 726, 730, 750, 754, 774, 798, 1008
CfoI GCGC 1 cut(s) 1006
Cfr10I RCCGGY 1 cut(s) 1013
Cfr13I GGNCC 9 cut(s) 144, 518, 710, 734, 758, 782, 806, 963, 971
CseI GACGC 1 cut(s) 245
Csp6I GTAC 2 cut(s) 163, 869
CviAII CATG 1 cut(s) 386
CviQI GTAC 2 cut(s) 163, 869
DdeI CTNAG 5 cut(s) 8, 302, 354, 372, 474
DpnI GATC 4 cut(s) 565, 894, 1025, 1101
DpnII GATC 4 cut(s) 563, 892, 1023, 1099
DrdI GACNNNNNNGTC 1 cut(s) 525
DriI GACNNNNNGTC 1 cut(s) 1042
DseDI GACNNNNNNGTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 225, 546
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 595
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 8 cut(s) 144, 518, 710, 734, 758, 782, 806, 971
Eco47III AGCGCT 1 cut(s) 1005
Eco57I CTGAAG 1 cut(s) 1047
Eco88I CYCGRG 2 cut(s) 87, 960
EcoO109I RGGNCCY 2 cut(s) 518, 971
EcoRI GAATTC 3 cut(s) 367, 427, 625
EcoRII CCWGG 6 cut(s) 43, 520, 736, 760, 884, 946
EcoRV GATATC 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 225, 546
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 2 cut(s) 225, 546
FaeI CATG 1 cut(s) 389
FaqI GGGAC 2 cut(s) 94, 334
FatI CATG 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 1011
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 1011
FspBI CTAG 2 cut(s) 825, 1080
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 1 cut(s) 1011
GsuI CTGGAG 3 cut(s) 576, 954, 1020
HaeII RGCGCY 1 cut(s) 1007
HaeIII GGCC 1 cut(s) 965
HapII CCGG 3 cut(s) 97, 966, 1014
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 1 cut(s) 242
HpaII CCGG 3 cut(s) 97, 966, 1014
HphI GGTGA 4 cut(s) 19, 535, 1033, 1043
Hpy166II GTNNAC 3 cut(s) 144, 782, 806
Hpy188I TCNGA 3 cut(s) 305, 373, 475
Hpy188III TCNNGA 5 cut(s) 186, 337, 825, 933, 1047
Hpy8I GTNNAC 3 cut(s) 144, 782, 806
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 5 cut(s) 479, 660, 831, 861, 1071
HpyCH4V TGCA 7 cut(s) 168, 182, 194, 268, 569, 638, 983
HpyF10VI GCNNNNNNNGC 6 cut(s) 382, 714, 738, 762, 1010, 1016
HpyF3I CTNAG 5 cut(s) 8, 302, 354, 372, 474
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 1 cut(s) 1004
Kzo9I GATC 4 cut(s) 563, 892, 1023, 1099
LmnI GCTCC 4 cut(s) 99, 405, 935, 1001
Lsp1109I GCAGC 1 cut(s) 1022
LweI GCATC 3 cut(s) 169, 191, 1066
MaeI CTAG 2 cut(s) 825, 1080
MaeIII GTNAC 3 cut(s) 527, 692, 1084
MalI GATC 4 cut(s) 565, 894, 1025, 1101
MboI GATC 4 cut(s) 563, 892, 1023, 1099
MboII GAAGA 2 cut(s) 407, 1018
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 2 cut(s) 96, 940
MluCI AATT 7 cut(s) 19, 35, 367, 413, 427, 614, 625
MmeI TCCRAC 2 cut(s) 456, 841
Mph1103I ATGCAT 1 cut(s) 184
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 3 cut(s) 97, 966, 1014
MspR9I CCNGG 6 cut(s) 45, 522, 738, 762, 886, 948
MunI CAATTG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 6 cut(s) 45, 522, 738, 762, 886, 948
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 6 cut(s) 382, 714, 738, 762, 1010, 1016
NdeII GATC 4 cut(s) 563, 892, 1023, 1099
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 527
NsiI ATGCAT 1 cut(s) 184
NspV TTCGAA 1 cut(s) 668
PaeR7I CTCGAG 1 cut(s) 87
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 1 cut(s) 242
PflMI CCANNNNNTGG 3 cut(s) 779, 803, 947
PkrI GCNGC 1 cut(s) 1012
PpuMI RGGWCCY 2 cut(s) 518, 971
Psp5II RGGWCCY 2 cut(s) 518, 971
Psp6I CCWGG 6 cut(s) 43, 520, 736, 760, 884, 946
PspGI CCWGG 6 cut(s) 43, 520, 736, 760, 884, 946
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 9 cut(s) 144, 518, 710, 734, 758, 782, 806, 963, 971
PspPPI RGGWCCY 2 cut(s) 518, 971
PspXI VCTCGAGB 1 cut(s) 87
PsuI RGATCY 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 2 cut(s) 164, 870
RsaNI GTAC 2 cut(s) 163, 869
SatI GCNGC 1 cut(s) 1011
Sau3AI GATC 4 cut(s) 563, 892, 1023, 1099
Sau96I GGNCC 9 cut(s) 144, 518, 710, 734, 758, 782, 806, 963, 971
ScaI AGTACT 1 cut(s) 164
ScrFI CCNGG 6 cut(s) 45, 522, 738, 762, 886, 948
SduI GDGCHC 2 cut(s) 96, 940
SfaNI GCATC 3 cut(s) 169, 191, 1066
SfcI CTRYAG 2 cut(s) 280, 314
Sfr274I CTCGAG 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 668
SinI GGWCC 8 cut(s) 144, 518, 710, 734, 758, 782, 806, 971
SlaI CTCGAG 1 cut(s) 87
SmlI CTYRAG 2 cut(s) 87, 441
SmoI CTYRAG 2 cut(s) 87, 441
Sse9I AATT 7 cut(s) 19, 35, 367, 413, 427, 614, 625
SsiI CCGC 6 cut(s) 239, 708, 732, 756, 839, 1019
SspMI CTAG 2 cut(s) 825, 1080
StyD4I CCNGG 6 cut(s) 43, 520, 736, 760, 884, 946
StyI CCWWGG 2 cut(s) 225, 546
TaqI TCGA 3 cut(s) 88, 653, 668
TasI AATT 7 cut(s) 19, 35, 367, 413, 427, 614, 625
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 1 cut(s) 242
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 1 cut(s) 1010
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 1 cut(s) 638
Van91I CCANNNNNTGG 3 cut(s) 779, 803, 947
VpaK11BI GGWCC 8 cut(s) 144, 518, 710, 734, 758, 782, 806, 971
XapI RAATTY 3 cut(s) 367, 427, 625
XbaI TCTAGA 1 cut(s) 824
XhoI CTCGAG 1 cut(s) 87
XspI CTAG 2 cut(s) 825, 1080
ZrmI AGTACT 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.