RchiOBHm_Chr1g0375891

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013557
Physical Location & Seq
Forward (+)
63197340 .. 63200415
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2325 bp
ATGAAAATGGTTCAAAAACCAGTGATCAAATGGCTGGTTCTGATTCTAACAAGCTGTGTCTTCTTCAGCATGGCCCTCTCTGCAAAAACCCAAACTTACATCATCCAAATGGACAAGTCCGCCAAGCCTGAATCCTTCACCACCCACCTCGATTGGTACTCATCCAAAGTAAACTCAGTACTCAACAAACCAGAAAAAGGAGACGATCAAAACCAAGACAGAATCATTTACACTTACCAAACCGCCTTTCATGGCGTCGCAGCTCAACTGAGCAAACAAGAAGCAGACCTACTAGAGCAACAAGACGGAGTCTTGGCTATATTCCCAGAACAGAAGTACCAGCTCCACACCACCAGAAGTCCTCTGTTTCTCGGGCTCGAACCACACGACAGCACCGCCGACAACGTCTGGTCCCAGCGAGTCTCTGACCATGACGTCATCGTAGGAGTACTCGACACGGGAGTCTGGCCGGAAAGCCAGAGCTTCAACGACACCGGAATGTCTCCGGTTCCGAGGCGCTGGCGCGGCGCATGCGAGACCGGCCGCGGCTTCACAAAAAGCAACTGCAACAGGAAAATCGTCGGCGCCAGAATCTTCCACCGGGGGTACGAGTCCGCCACCGGAAAAATCAACGAGAAGATGGAGTACAAGTCGCCAAGGGACCAGGACGGACACGGGACCCACACCGCCGCAACCGTCGCCGGATCTCCCGTACGTGGCGCCAATCTTCTCGGCTACGCGTACGGTACGGCGAGAGGAATGGCGCCGGCGGCGAGGATCGCGGCGTACAAGGTGTGCTGGGTTGGAGGTTGCTTCAGCTCTGATATAATGGCAGCGGTTGATAAGGCCGTCGAGGACGGTGTCGATGTTTTGTCTATTTCTCTTGGTGGTGGAGTTTCCTCGTATTACCGGGATAGCTTGTCTATTGCGGCGTTTGGTGCGATGGAGATGGGAGTTTTTGTCTCTTGCTCCGCCGGGAACGGTGGGCCAGACCCGGTGAGTCTGACTAATGTGTCACCTTGGATCACCACCGTCGGTGCTAGCACTATGGATAGGGATTTTCCGGCCACTGTGAAGTTGGGGAATGGGAGGACTATTACTGGAGTTTCGCTTTATAGAGGGAGAATGAAGCTTGACGCAAAGAAGCTGTACCCTGTTGTGTTAGCGGGTGGGAATTCGAGTAGCCCCAATCCGAGCTCGTTGTGTTTAGAGGGTACTTTAGATCCGAAGAAAGTTAAGGGGAAGATTGTGATTTGTGATCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGACGTGGTGAAGAATGCCGGAGGAGTTGGGATGATCTTGGCTAATACGGCTTCGAATGGGGAGGAGCTGGTTGCTGATTGTCATCTGATTCCGGCGGTTGCGGTGGGAGAGAATGAAGCAAAATCGATCAAGCATTACGCCTTAACAAGTCCGAAAGCAAAGGCAACTCTAGCATTTGTTGGTACTAGACTAGGGGTTAGACCGTCTCCCATTGTGGCTGCATTTTCGTCAAGAGGGCCTAATCTTTTGAGTTTTGAGATTATGAAGCCGGATTTGGTGGCGCCGGGAGTGAACATTATTGCTGCTTGGACTGGGGCATTGGGTCCGTCGAGCTTGGCAACGGATCGCAGGAGAGTGAAGTTCAATATCTTGTCCGGGACTTCAATGTCGTGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAATCTAGGCACCCCGAATGGAGTCCAGCCGCGATCAAATCCGCCTTGATGACTACAGCTTATGTTCATGACAACACACATAAGCCTCTGGAAGATGCCTCAACAGCTAAAATTTCAACCCCTTTTGATCATGGAGCTGGACACATAAACCCGAGGAAGGCTCTTGACCCGGGCTTGGTTTATGACATTCAGCCCCAGGATTATCTTGAGTTCCTCTGCACACAAAAACTAACCCCAACGCAGCTCAAGGTGTTTAGCAATAGCACTTGCCGTCACACTCTGGCCAATGCAGGGGACCTCAACTATCCCGCAATTTCCGTTGTGTTTGTGGAGAACACTAATGTCTCTGTCTTGACAGTTCATAGAACTGTCACTAATGTTGGCCCTGCGGTTTCGAGTTACCATGCCATAGTTTCGCCATTCAAGGGTGCTTATGTGAAAGTTGAGCCGCGAATTCTGAAGTTCACCAAAGCTAATCAGAAGCTGTCTTACAAAGTGACCTTTACTACAAAGACTAGGCAGACGGAACCTGAGTTTGGAGGTTTGGTGTGGAAGGCTGGAGTCCACAGAGTTAGAAGTCCAATTGTTGTGGCATGGGGGATGCTGATATGA

Protein Analysis

774

Amino Acids

83.37

Weight (kDa)

9.3

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 116 1.6e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 144 - 597 9.1e-51 Subtilase family
PA PF02225 384 - 473 1.7e-13 PA domain
fn3_6 PF17766 670 - 768 1e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 434, 461, 1012, 1675
AatII GACGTC 1 cut(s) 438
AccB1I GGYRCC 5 cut(s) 584, 719, 763, 1570, 1722
AccII CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
AclWI GGATC 5 cut(s) 712, 785, 1031, 1217, 1641
AcoI YGGCCR 4 cut(s) 467, 541, 1065, 1995
AcsI RAATTY 3 cut(s) 1174, 1824, 2166
AcuI CTGAAG 3 cut(s) 49, 799, 2192
AcyI GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
AfiI CCNNNNNNNGG 7 cut(s) 197, 716, 1870, 1888, 2004, 2138, 2249
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 6 cut(s) 14, 487, 1654, 1674, 1830, 2137
AjiI CACGTC 1 cut(s) 1294
AjnI CCWGG 2 cut(s) 663, 1908
AjuI GAANNNNNNNTTGG 6 cut(s) 231, 263, 1547, 1579, 2232, 2264
Alw21I GWGCWC 1 cut(s) 1199
Alw26I GTCTC 8 cut(s) 195, 427, 507, 530, 967, 1284, 1500, 2062
AlwI GGATC 5 cut(s) 712, 785, 1031, 1217, 1641
AlwNI CAGNNNCTG 1 cut(s) 2197
Ama87I CYCGRG 3 cut(s) 371, 1864, 1882
AoxI GGCC 9 cut(s) 72, 467, 541, 846, 986, 1065, 1526, 1995, 2095
ApeKI GCWGC 6 cut(s) 260, 833, 1508, 1592, 1703, 1954
ApoI RAATTY 3 cut(s) 1174, 1824, 2166
AspLEI GCGC 7 cut(s) 519, 525, 530, 587, 722, 766, 1573
AspS9I GGNCC 9 cut(s) 73, 411, 661, 678, 986, 1526, 1613, 2008, 2096
AsuC2I CCSGG 8 cut(s) 602, 911, 976, 995, 1575, 1666, 1883, 1884
AsuHPI GGTGA 6 cut(s) 130, 1008, 1009, 1018, 1309, 2170
AsuII TTCGAA 1 cut(s) 1343
AsuNHI GCTAGC 1 cut(s) 1040
AvaI CYCGRG 3 cut(s) 371, 1864, 1882
AvaII GGWCC 5 cut(s) 411, 661, 678, 1613, 2008
BaeI ACNNNNGTAYC 2 cut(s) 320, 353
BalI TGGCCA 1 cut(s) 1997
BanI GGYRCC 5 cut(s) 584, 719, 763, 1570, 1722
BanII GRGCYC 2 cut(s) 378, 1199
BarI GAAGNNNNNNTAC 6 cut(s) 273, 305, 629, 661, 2186, 2218
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 1199
BbvI GCAGC 6 cut(s) 272, 845, 1495, 1579, 1690, 1966
BccI CCATC 3 cut(s) 634, 937, 943
BceAI ACGGC 4 cut(s) 765, 833, 1353, 1968
BciT130I CCWGG 2 cut(s) 665, 1910
BclI TGATCA 2 cut(s) 24, 1840
BcnI CCSGG 8 cut(s) 602, 911, 976, 995, 1575, 1666, 1883, 1884
BcoDI GTCTC 8 cut(s) 195, 427, 507, 530, 967, 1284, 1500, 2062
BfaI CTAG 8 cut(s) 293, 1041, 1275, 1460, 1476, 1481, 1719, 2229
BfmI CTRYAG 1 cut(s) 1767
BfoI RGCGCY 5 cut(s) 520, 588, 723, 767, 1574
BmcAI AGTACT 2 cut(s) 180, 450
Bme18I GGWCC 5 cut(s) 411, 661, 678, 1613, 2008
BmeT110I CYCGRG 3 cut(s) 371, 1864, 1882
BmgBI CACGTC 1 cut(s) 1294
BmgT120I GGNCC 9 cut(s) 73, 411, 661, 678, 986, 1526, 1613, 2008, 2096
BmrI ACTGGG 1 cut(s) 1611
BmsI GCATC 2 cut(s) 1798, 2304
BmtI GCTAGC 1 cut(s) 1044
BmuI ACTGGG 1 cut(s) 1611
BpiI GAAGAC 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 1858, 1890
BpmI CTGGAG 2 cut(s) 1122, 2292
Bpu14I TTCGAA 1 cut(s) 1343
BpuEI CTTGAG 2 cut(s) 1940, 1943
BpuMI CCSGG 8 cut(s) 602, 911, 976, 995, 1575, 1666, 1883, 1884
Bsa29I ATCGAT 1 cut(s) 1415
BsaAI YACGTR 1 cut(s) 716
BsaBI GATNNNNATC 1 cut(s) 1371
BsaHI GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 8 cut(s) 512, 544, 601, 656, 1019, 1865, 1882, 1908
BsaWI WCCGGW 3 cut(s) 494, 505, 620
BsaXI ACNNNNNCTCC 8 cut(s) 635, 665, 945, 975, 1281, 1311, 2244, 2274
Bsc4I CCNNNNNNNGG 7 cut(s) 197, 716, 1870, 1888, 2004, 2138, 2249
Bse118I RCCGGY 2 cut(s) 539, 766
Bse1I ACTGG 3 cut(s) 20, 1105, 1606
Bse8I GATNNNNATC 1 cut(s) 1371
BseBI CCWGG 2 cut(s) 665, 1910
BseCI ATCGAT 1 cut(s) 1415
BseDI CCNNGG 8 cut(s) 512, 544, 601, 656, 1019, 1865, 1882, 1908
BseGI GGATG 4 cut(s) 102, 161, 1326, 2319
BseJI GATNNNNATC 1 cut(s) 1371
BseLI CCNNNNNNNGG 7 cut(s) 197, 716, 1870, 1888, 2004, 2138, 2249
BseMII CTCAG 3 cut(s) 189, 260, 2235
BseNI ACTGG 3 cut(s) 20, 1105, 1606
BseRI GAGGAG 2 cut(s) 1326, 1367
BseX3I CGGCCG 1 cut(s) 541
BseXI GCAGC 6 cut(s) 272, 845, 1495, 1579, 1690, 1966
BseYI CCCAGC 2 cut(s) 414, 798
BsgI GTGCAG 2 cut(s) 1301, 1915
Bsh1236I CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
Bsh1285I CGRYCG 1 cut(s) 544
BshFI GGCC 9 cut(s) 74, 469, 543, 848, 988, 1067, 1528, 1997, 2097
BshNI GGYRCC 5 cut(s) 584, 719, 763, 1570, 1722
BshVI ATCGAT 1 cut(s) 1415
BsiEI CGRYCG 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 1199
BsiHKCI CYCGRG 3 cut(s) 371, 1864, 1882
BsiWI CGTACG 2 cut(s) 712, 741
BslFI GGGAC 5 cut(s) 397, 674, 691, 1681, 2021
BslI CCNNNNNNNGG 7 cut(s) 197, 716, 1870, 1888, 2004, 2138, 2249
BsmAI GTCTC 8 cut(s) 195, 427, 507, 530, 967, 1284, 1500, 2062
BsmBI CGTCTC 3 cut(s) 195, 1284, 1500
BsmFI GGGAC 5 cut(s) 397, 674, 691, 1681, 2021
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 9 cut(s) 74, 469, 543, 848, 988, 1067, 1528, 1997, 2097
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 3 cut(s) 371, 1864, 1882
Bsp119I TTCGAA 1 cut(s) 1343
Bsp1286I GDGCHC 2 cut(s) 378, 1199
BspANI GGCC 9 cut(s) 74, 469, 543, 848, 988, 1067, 1528, 1997, 2097
BspCNI CTCAG 3 cut(s) 188, 261, 2236
BspDI ATCGAT 1 cut(s) 1415
BspFNI CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
BspHI TCATGA 1 cut(s) 1780
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 5 cut(s) 712, 785, 1031, 1217, 1641
BspT104I TTCGAA 1 cut(s) 1343
BspT107I GGYRCC 5 cut(s) 584, 719, 763, 1570, 1722
BspTNI GGTCTC 1 cut(s) 530
BsrFI RCCGGY 2 cut(s) 539, 766
BsrI ACTGG 3 cut(s) 20, 1105, 1606
BssAI RCCGGY 2 cut(s) 539, 766
BssECI CCNNGG 8 cut(s) 512, 544, 601, 656, 1019, 1865, 1882, 1908
BssNI GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
BssT1I CCWWGG 2 cut(s) 656, 1019
Bst2UI CCWGG 2 cut(s) 665, 1910
Bst4CI ACNGT 9 cut(s) 697, 746, 860, 983, 1033, 1072, 1494, 2071, 2083
BstACI GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
BstBAI YACGTR 1 cut(s) 716
BstBI TTCGAA 1 cut(s) 1343
BstC8I GCNNGC 4 cut(s) 521, 532, 768, 1042
BstDEI CTNAG 3 cut(s) 175, 269, 2244
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 4 cut(s) 102, 161, 1326, 2319
BstFNI CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
BstH2I RGCGCY 5 cut(s) 520, 588, 723, 767, 1574
BstHHI GCGC 7 cut(s) 519, 525, 530, 587, 722, 766, 1573
BstMAI GTCTC 8 cut(s) 195, 427, 507, 530, 967, 1284, 1500, 2062
BstMCI CGRYCG 1 cut(s) 544
BstNI CCWGG 2 cut(s) 665, 1910
BstNSI RCATGY 1 cut(s) 534
BstSFI CTRYAG 1 cut(s) 1767
BstUI CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
BstV1I GCAGC 6 cut(s) 272, 845, 1495, 1579, 1690, 1966
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 2 cut(s) 704, 1222
BstYI RGATCY 2 cut(s) 704, 1222
BstZI CGGCCG 1 cut(s) 541
Bsu15I ATCGAT 1 cut(s) 1415
BsuRI GGCC 9 cut(s) 74, 469, 543, 848, 988, 1067, 1528, 1997, 2097
BsuTUI ATCGAT 1 cut(s) 1415
BtgI CCRYGG 1 cut(s) 544
BtgZI GCGATG 1 cut(s) 956
BtrI CACGTC 1 cut(s) 1294
BtsCI GGATG 4 cut(s) 102, 161, 1326, 2319
BtsIMutI CAGTG 2 cut(s) 27, 1068
Cac8I GCNNGC 4 cut(s) 521, 532, 768, 1042
CaiI CAGNNNCTG 1 cut(s) 2197
CciI TCATGA 1 cut(s) 1780
CfoI GCGC 7 cut(s) 519, 525, 530, 587, 722, 766, 1573
Cfr10I RCCGGY 2 cut(s) 539, 766
Cfr13I GGNCC 9 cut(s) 73, 411, 661, 678, 986, 1526, 1613, 2008, 2096
Cfr42I CCGCGG 1 cut(s) 547
Cfr9I CCCGGG 1 cut(s) 1882
ClaI ATCGAT 1 cut(s) 1415
CseI GACGC 2 cut(s) 244, 1145
CspCI CAANNNNNGTGG 4 cut(s) 134, 169, 2283, 2318
CviAII CATG 9 cut(s) 70, 251, 431, 531, 1688, 1781, 1844, 2117, 2307
DdeI CTNAG 3 cut(s) 175, 269, 2244
DinI GGCGCC 4 cut(s) 586, 721, 765, 1572
DrdI GACNNNNNNGTC 4 cut(s) 434, 461, 1012, 1675
DseDI GACNNNNNNGTC 4 cut(s) 434, 461, 1012, 1675
EaeI YGGCCR 4 cut(s) 467, 541, 1065, 1995
EagI CGGCCG 1 cut(s) 541
EciI GGCGGA 4 cut(s) 109, 604, 961, 1744
Ecl136II GAGCTC 1 cut(s) 1197
EclXI CGGCCG 1 cut(s) 541
Eco130I CCWWGG 2 cut(s) 656, 1019
Eco24I GRGCYC 2 cut(s) 378, 1199
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 5 cut(s) 411, 661, 678, 1613, 2008
Eco52I CGGCCG 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 1197
Eco57I CTGAAG 3 cut(s) 49, 799, 2192
Eco88I CYCGRG 3 cut(s) 371, 1864, 1882
EcoICRI GAGCTC 1 cut(s) 1197
EcoO109I RGGNCCY 3 cut(s) 678, 1526, 2008
EcoRI GAATTC 2 cut(s) 1174, 2166
EcoRII CCWGG 2 cut(s) 663, 1908
EcoT14I CCWWGG 2 cut(s) 656, 1019
EcoT38I GRGCYC 2 cut(s) 378, 1199
EgeI GGCGCC 4 cut(s) 586, 721, 765, 1572
EheI GGCGCC 4 cut(s) 586, 721, 765, 1572
ErhI CCWWGG 2 cut(s) 656, 1019
Esp3I CGTCTC 3 cut(s) 195, 1284, 1500
FaeI CATG 9 cut(s) 73, 254, 434, 534, 1691, 1784, 1847, 2120, 2310
FaqI GGGAC 5 cut(s) 397, 674, 691, 1681, 2021
FatI CATG 9 cut(s) 69, 250, 430, 530, 1687, 1780, 1843, 2116, 2306
FauI CCCGC 2 cut(s) 1159, 2029
FbaI TGATCA 2 cut(s) 24, 1840
FokI GGATG 3 cut(s) 89, 148, 1333
FriOI GRGCYC 2 cut(s) 378, 1199
FspBI CTAG 8 cut(s) 293, 1041, 1275, 1460, 1476, 1481, 1719, 2229
GlaI GCGC 7 cut(s) 518, 524, 529, 586, 721, 765, 1572
GsaI CCCAGC 2 cut(s) 418, 802
GsuI CTGGAG 2 cut(s) 1122, 2292
HaeII RGCGCY 5 cut(s) 520, 588, 723, 767, 1574
HaeIII GGCC 9 cut(s) 74, 469, 543, 848, 988, 1067, 1528, 1997, 2097
HgaI GACGC 2 cut(s) 244, 1145
HhaI GCGC 7 cut(s) 519, 525, 530, 587, 722, 766, 1573
Hin1I GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
Hin1II CATG 9 cut(s) 73, 254, 434, 534, 1691, 1784, 1847, 2120, 2310
Hin6I GCGC 7 cut(s) 517, 523, 528, 585, 720, 764, 1571
HinP1I GCGC 7 cut(s) 517, 523, 528, 585, 720, 764, 1571
HindIII AAGCTT 1 cut(s) 1130
HphI GGTGA 6 cut(s) 130, 1008, 1009, 1018, 1309, 2170
Hpy166II GTNNAC 4 cut(s) 172, 1582, 2178, 2278
Hpy188III TCNNGA 6 cut(s) 1521, 1781, 1802, 1877, 1919, 2065
Hpy8I GTNNAC 4 cut(s) 172, 1582, 2178, 2278
Hpy99I CGWCG 6 cut(s) 260, 584, 701, 854, 1037, 1621
HpyAV CCTTC 4 cut(s) 145, 1279, 1864, 2260
HpyCH4III ACNGT 9 cut(s) 697, 746, 860, 983, 1033, 1072, 1494, 2071, 2083
HpyCH4IV ACGT 4 cut(s) 405, 435, 715, 1293
HpyCH4V TGCA 6 cut(s) 83, 567, 1282, 1511, 1932, 2003
HpyF3I CTNAG 3 cut(s) 175, 269, 2244
HpySE526I ACGT 4 cut(s) 405, 435, 715, 1293
Hsp92I GRCGYC 6 cut(s) 255, 435, 585, 720, 764, 1571
Hsp92II CATG 9 cut(s) 73, 254, 434, 534, 1691, 1784, 1847, 2120, 2310
HspAI GCGC 7 cut(s) 517, 523, 528, 585, 720, 764, 1571
KasI GGCGCC 4 cut(s) 584, 719, 763, 1570
KflI GGGWCCC 1 cut(s) 678
KroI GCCGGC 1 cut(s) 766
KroNI GCCGGC 1 cut(s) 768
Ksp22I TGATCA 2 cut(s) 24, 1840
KspI CCGCGG 1 cut(s) 547
LmnI GCTCC 4 cut(s) 348, 974, 1354, 1847
Lsp1109I GCAGC 6 cut(s) 272, 845, 1495, 1579, 1690, 1966
LweI GCATC 2 cut(s) 1798, 2304
MaeI CTAG 8 cut(s) 293, 1041, 1275, 1460, 1476, 1481, 1719, 2229
MaeII ACGT 4 cut(s) 405, 435, 715, 1293
MaeIII GTNAC 5 cut(s) 1014, 1985, 2083, 2111, 2209
MboII GAAGA 9 cut(s) 52, 55, 586, 649, 719, 1240, 1255, 1312, 1817
MfeI CAATTG 1 cut(s) 2295
MflI RGATCY 2 cut(s) 704, 1222
MhlI GDGCHC 2 cut(s) 378, 1199
MlsI TGGCCA 1 cut(s) 1997
MluCI AATT 5 cut(s) 1174, 1824, 2025, 2166, 2295
MluI ACGCGT 1 cut(s) 738
MluNI TGGCCA 1 cut(s) 1997
Mly113I GGCGCC 4 cut(s) 585, 720, 764, 1571
MlyI GAGTC 7 cut(s) 318, 429, 471, 620, 1009, 1744, 2283
MmeI TCCRAC 1 cut(s) 784
Mox20I TGGCCA 1 cut(s) 1997
MreI CGCCGGCG 1 cut(s) 766
MroNI GCCGGC 1 cut(s) 766
MscI TGGCCA 1 cut(s) 1997
MseI TTAA 2 cut(s) 1236, 1433
MslI CAYNNNNRTG 3 cut(s) 107, 497, 1692
Msp20I TGGCCA 1 cut(s) 1997
MspA1I CMGCKG 2 cut(s) 546, 836
MteI GCGCNGCGC 1 cut(s) 526
MunI CAATTG 1 cut(s) 2295
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 2 cut(s) 665, 1910
MvnI CGCG 6 cut(s) 525, 546, 740, 782, 1745, 2164
NaeI GCCGGC 1 cut(s) 768
NarI GGCGCC 4 cut(s) 585, 720, 764, 1571
NciI CCSGG 8 cut(s) 602, 911, 976, 995, 1575, 1666, 1883, 1884
NgoMIV GCCGGC 1 cut(s) 766
NheI GCTAGC 1 cut(s) 1040
NlaIII CATG 9 cut(s) 73, 254, 434, 534, 1691, 1784, 1847, 2120, 2310
NmeAIII GCCGAG 1 cut(s) 711
NmuCI GTSAC 4 cut(s) 1014, 1985, 2083, 2209
NspI RCATGY 1 cut(s) 534
NspV TTCGAA 1 cut(s) 1343
PaeI GCATGC 1 cut(s) 534
PagI TCATGA 1 cut(s) 1780
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PctI GAATGC 1 cut(s) 1309
PdiI GCCGGC 1 cut(s) 768
PfeI GAWTC 5 cut(s) 43, 131, 222, 591, 1378
Pfl23II CGTACG 2 cut(s) 712, 741
PflFI GACNNNGTC 3 cut(s) 308, 404, 860
PfoI TCCNGGA 1 cut(s) 1664
PleI GAGTC 7 cut(s) 317, 428, 470, 619, 1008, 1743, 2282
PluTI GGCGCC 4 cut(s) 588, 723, 767, 1574
PpsI GAGTC 7 cut(s) 317, 428, 470, 619, 1008, 1743, 2282
Ppu21I YACGTR 1 cut(s) 716
PpuMI RGGWCCY 2 cut(s) 678, 2008
Psp124BI GAGCTC 1 cut(s) 1199
Psp5II RGGWCCY 2 cut(s) 678, 2008
Psp6I CCWGG 2 cut(s) 663, 1908
PspFI CCCAGC 2 cut(s) 414, 798
PspGI CCWGG 2 cut(s) 663, 1908
PspLI CGTACG 2 cut(s) 712, 741
PspPI GGNCC 9 cut(s) 73, 411, 661, 678, 986, 1526, 1613, 2008, 2096
PspPPI RGGWCCY 2 cut(s) 678, 2008
PstNI CAGNNNCTG 1 cut(s) 2197
PsuI RGATCY 2 cut(s) 704, 1222
PsyI GACNNNGTC 3 cut(s) 308, 404, 860
RseI CAYNNNNRTG 3 cut(s) 107, 497, 1692
SacI GAGCTC 1 cut(s) 1199
SacII CCGCGG 1 cut(s) 547
SaqAI TTAA 2 cut(s) 1236, 1433
Sau96I GGNCC 9 cut(s) 73, 411, 661, 678, 986, 1526, 1613, 2008, 2096
ScaI AGTACT 2 cut(s) 180, 450
SchI GAGTC 7 cut(s) 318, 429, 471, 620, 1009, 1744, 2283
SduI GDGCHC 2 cut(s) 378, 1199
SfaNI GCATC 2 cut(s) 1798, 2304
SfcI CTRYAG 1 cut(s) 1767
SfoI GGCGCC 4 cut(s) 586, 721, 765, 1572
Sfr303I CCGCGG 1 cut(s) 547
SfuI TTCGAA 1 cut(s) 1343
SgrAI CRCCGGYG 1 cut(s) 766
SgrBI CCGCGG 1 cut(s) 547
SinI GGWCC 5 cut(s) 411, 661, 678, 1613, 2008
SmaI CCCGGG 1 cut(s) 1884
SmiMI CAYNNNNRTG 3 cut(s) 107, 497, 1692
SmlI CTYRAG 2 cut(s) 1919, 1958
SmoI CTYRAG 2 cut(s) 1919, 1958
SphI GCATGC 1 cut(s) 534
Sse9I AATT 5 cut(s) 1174, 1824, 2025, 2166, 2295
SspDI GGCGCC 4 cut(s) 584, 719, 763, 1570
SspMI CTAG 8 cut(s) 293, 1041, 1275, 1460, 1476, 1481, 1719, 2229
SstI GAGCTC 1 cut(s) 1199
StyI CCWWGG 2 cut(s) 656, 1019
TaaI ACNGT 9 cut(s) 697, 746, 860, 983, 1033, 1072, 1494, 2071, 2083
TaiI ACGT 4 cut(s) 408, 438, 718, 1296
TasI AATT 5 cut(s) 1174, 1824, 2025, 2166, 2295
TatI WGTACW 3 cut(s) 178, 448, 645
TauI GCSGC 9 cut(s) 528, 546, 549, 692, 773, 785, 932, 1745, 2164
TfiI GAWTC 5 cut(s) 43, 131, 222, 591, 1378
Tru1I TTAA 2 cut(s) 1236, 1433
Tru9I TTAA 2 cut(s) 1236, 1433
TscAI CASTG 2 cut(s) 27, 1075
TseFI GTSAC 4 cut(s) 1014, 1985, 2083, 2209
TseI GCWGC 6 cut(s) 260, 833, 1508, 1592, 1703, 1954
Tsp45I GTSAC 4 cut(s) 1014, 1985, 2083, 2209
TspDTI ATGAA 7 cut(s) 17, 239, 1142, 1419, 1568, 1769, 2063
TspGWI ACGGA 6 cut(s) 321, 684, 1605, 1646, 2020, 2252
TspMI CCCGGG 1 cut(s) 1882
TspRI CASTG 2 cut(s) 27, 1075
Tth111I GACNNNGTC 3 cut(s) 308, 404, 860
VpaK11BI GGWCC 5 cut(s) 411, 661, 678, 1613, 2008
XapI RAATTY 3 cut(s) 1174, 1824, 2166
XceI RCATGY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 2 cut(s) 27, 1075
XmaI CCCGGG 1 cut(s) 1882
XspI CTAG 8 cut(s) 293, 1041, 1275, 1460, 1476, 1481, 1719, 2229
ZraI GACGTC 1 cut(s) 436
ZrmI AGTACT 2 cut(s) 180, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.