RchiOBHm_Chr1g0381031

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008674
Physical Location & Seq
Forward (+)
66432547 .. 66435003
2457 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGAAAACTCTCAGAGCCATCATCTTCTGCTTCCTTCTCTTCTCTTCATTCTCATGCCTTAAAACGAGTAGTACCTTAGAGACTTGCAAAAACGCTGTTTGCCAATCCAGCGAGGCCGTAATCCGTTTCCCGTTTCGGATCGACAAACGCCAGCCGGAGACTTGTGGCTTCCCCGGTTTTGATCTGACCTGTGACATGACATCCCAAACACTGCTCAATCTTCCCTCTTCGGGGGATTTCACCGTTCAGGGCATAGACTACGGCGAGCAAGAGATATGGATCAACGATCCAAACACCTGCCTCCCCAAACGGCTCCTCTCTCTGAACCTCACCGGCTCTCCGTTTCGGGCTGGGTTTAGTGAAGACTTCACGTTCTTTAACTGCTCCTTGGACTACTTGAAGTACAAGCTAAACCCTATTGCTTGCATGAGTGGTTCCAACTACACGGTTTTCGCCACCAATTCTGCGAGGGTGATTACGTACTTGTCGACGTGCGGTAAGATTGGAACGTTTTCTGTTCCGGTCGAGTGGACGTTTCACCAGGAGGTTATGTCGTCGGACCTCGACGATCATCTCCGGCTAAAGTGGGACCGACCGGGGTGCGGCAGCTGCGAGTTGCGTGGCGGAAAGTGTGGCTTCAAGACCAATTCTACTAAAGTTGTTTGCTCCAGCCTTAATCAACGAGGAATCCCAAGGGGTGCACGCTATGCTATTACAGTGGGCGTTGGAGTGCCAGCAATTATGTGCCTTATAGGGCTATTATGTTGCATGTGTGGCAGACTCAAGCCTTTAACAATGAGACGCACATCACTCCCAGAATTTAATTCAATTGGGGCTCCGCAGCCTGGAATCGTAAAGGGACTCGATGTGGCAACACTTGCGGCTTACCCGAAAATAGTCCTCAGTGAAAGCCGGCGTCTACCGCAGCCGGATGATAGCACTTGTTCGATATGCTTGTCCGACTACCGGCCGAAGGAGATACTGAAGACCATACCCGAATGCCAACATTGCTTCCATGCTAATTGCATCGATGAATGGCTTAAATTGAATGCTACTTGCCCTATTTGTAGAAAATCTCCACAATGA

Protein Analysis

361

Amino Acids

39.99

Weight (kDa)

8.52

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 96 1.4e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 155 - 223 2.1e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 314 - 357 1.6e-12 Ring finger domain
zf-rbx1 PF12678 314 - 357 6.7e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 315 - 343 2.7e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 315 - 356 7.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 305
AccI GTMKAC 2 cut(s) 488, 919
AciI CCGC 6 cut(s) 495, 603, 624, 839, 881, 923
AclI AACGTT 1 cut(s) 509
AclWI GGATC 3 cut(s) 146, 281, 287
AcoI YGGCCR 1 cut(s) 968
AcsI RAATTY 1 cut(s) 818
AcuI CTGAAG 1 cut(s) 1004
AcyI GRCGYC 1 cut(s) 916
AfaI GTAC 3 cut(s) 73, 404, 482
AfiI CCNNNNNNNGG 6 cut(s) 230, 231, 602, 845, 966, 973
AgsI TTSAA 4 cut(s) 400, 640, 828, 1048
AjiI CACGTC 1 cut(s) 492
AjnI CCWGG 2 cut(s) 540, 844
AluBI AGCT 2 cut(s) 409, 609
AluI AGCT 2 cut(s) 409, 609
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 3 cut(s) 74, 152, 793
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 3 cut(s) 146, 281, 287
AoxI GGCC 2 cut(s) 114, 968
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 4 cut(s) 606, 609, 841, 925
ApoI RAATTY 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 559, 589
AsuC2I CCSGG 2 cut(s) 174, 597
AsuHPI GGTGA 4 cut(s) 232, 322, 484, 530
AvaII GGWCC 2 cut(s) 559, 589
BaeGI GKGCMC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 838
BbsI GAAGAC 2 cut(s) 369, 992
Bbv12I GWGCWC 1 cut(s) 703
BbvI GCAGC 4 cut(s) 596, 618, 853, 937
BccI CCATC 1 cut(s) 26
BceAI ACGGC 3 cut(s) 101, 277, 326
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciT130I CCWGG 2 cut(s) 542, 846
BcnI CCSGG 2 cut(s) 174, 597
BcoDI GTCTC 3 cut(s) 74, 152, 793
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 6 cut(s) 604, 607, 610, 842, 882, 926
BlsI GCNGC 6 cut(s) 605, 608, 611, 843, 883, 927
Bme1390I CCNGG 4 cut(s) 174, 542, 597, 846
Bme18I GGWCC 2 cut(s) 559, 589
BmgBI CACGTC 1 cut(s) 492
BmgT120I GGNCC 2 cut(s) 559, 589
BmiI GGNNCC 4 cut(s) 314, 436, 590, 837
BmrFI CCNGG 4 cut(s) 174, 542, 597, 846
BmsI GCATC 1 cut(s) 1035
BpiI GAAGAC 2 cut(s) 369, 992
BpmI CTGGAG 1 cut(s) 652
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 174, 597
Bsa29I ATCGAT 1 cut(s) 1029
BsaAI YACGTR 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 916
BsaJI CCNNGG 4 cut(s) 172, 387, 596, 692
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 536, 566
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 231, 602, 845, 966, 973
Bse118I RCCGGY 3 cut(s) 332, 912, 966
Bse3DI GCAATG 1 cut(s) 1006
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 2 cut(s) 542, 846
BseCI ATCGAT 1 cut(s) 1029
BseDI CCNNGG 4 cut(s) 172, 387, 596, 692
BseGI GGATG 2 cut(s) 200, 937
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 6 cut(s) 230, 231, 602, 845, 966, 973
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 2 cut(s) 25, 916
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 703
BseX3I CGGCCG 1 cut(s) 968
BseXI GCAGC 4 cut(s) 596, 618, 853, 937
BseYI CCCAGC 1 cut(s) 350
Bsh1285I CGRYCG 3 cut(s) 525, 596, 971
BshFI GGCC 2 cut(s) 116, 970
BshVI ATCGAT 1 cut(s) 1029
BsiEI CGRYCG 3 cut(s) 525, 596, 971
BsiHKAI GWGCWC 1 cut(s) 703
BsiSI CCGG 9 cut(s) 155, 174, 333, 521, 577, 596, 913, 929, 967
BslFI GGGAC 2 cut(s) 602, 873
BslI CCNNNNNNNGG 6 cut(s) 230, 231, 602, 845, 966, 973
BsmAI GTCTC 3 cut(s) 74, 152, 793
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 2 cut(s) 602, 873
BsmI GAATGC 2 cut(s) 1004, 1054
BsnI GGCC 2 cut(s) 116, 970
Bsp1286I GDGCHC 2 cut(s) 703, 838
Bsp143I GATC 5 cut(s) 138, 181, 279, 286, 568
BspACI CCGC 6 cut(s) 495, 603, 624, 839, 881, 923
BspANI GGCC 2 cut(s) 116, 970
BspCNI CTCAG 2 cut(s) 24, 915
BspDI ATCGAT 1 cut(s) 1029
BspLI GGNNCC 4 cut(s) 314, 436, 590, 837
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 3 cut(s) 146, 281, 287
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 3 cut(s) 332, 912, 966
BssAI RCCGGY 3 cut(s) 332, 912, 966
BssECI CCNNGG 4 cut(s) 172, 387, 596, 692
BssMI GATC 5 cut(s) 138, 181, 279, 286, 568
BssNI GRCGYC 1 cut(s) 916
BssT1I CCWWGG 2 cut(s) 387, 692
Bst2UI CCWGG 2 cut(s) 542, 846
Bst4CI ACNGT 3 cut(s) 244, 448, 718
Bst6I CTCTTC 3 cut(s) 44, 49, 232
BstACI GRCGYC 1 cut(s) 916
BstAPI GCANNNNNTGC 2 cut(s) 707, 878
BstBAI YACGTR 1 cut(s) 480
BstC8I GCNNGC 6 cut(s) 152, 266, 424, 703, 735, 914
BstDEI CTNAG 3 cut(s) 11, 76, 902
BstF5I GGATG 2 cut(s) 200, 937
BstKTI GATC 5 cut(s) 141, 184, 282, 289, 571
BstMAI GTCTC 3 cut(s) 74, 152, 793
BstMBI GATC 5 cut(s) 138, 181, 279, 286, 568
BstMCI CGRYCG 3 cut(s) 525, 596, 971
BstMWI GCNNNNNNNGC 7 cut(s) 108, 609, 707, 774, 878, 922, 1008
BstNI CCWGG 2 cut(s) 542, 846
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 4 cut(s) 172, 540, 595, 844
BstSLI GKGCMC 1 cut(s) 703
BstSNI TACGTA 1 cut(s) 480
BstV1I GCAGC 4 cut(s) 596, 618, 853, 937
BstV2I GAAGAC 2 cut(s) 369, 992
BstZI CGGCCG 1 cut(s) 968
Bsu15I ATCGAT 1 cut(s) 1029
BsuRI GGCC 2 cut(s) 116, 970
BsuTUI ATCGAT 1 cut(s) 1029
BtrI CACGTC 1 cut(s) 492
BtsCI GGATG 2 cut(s) 200, 937
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 3 cut(s) 209, 723, 910
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 6 cut(s) 152, 266, 424, 703, 735, 914
Cfr10I RCCGGY 3 cut(s) 332, 912, 966
Cfr13I GGNCC 2 cut(s) 559, 589
ClaI ATCGAT 1 cut(s) 1029
CseI GACGC 2 cut(s) 810, 905
Csp6I GTAC 3 cut(s) 72, 403, 481
CviAII CATG 5 cut(s) 54, 196, 427, 769, 1016
CviQI GTAC 3 cut(s) 72, 403, 481
DdeI CTNAG 3 cut(s) 11, 76, 902
DpnI GATC 5 cut(s) 140, 183, 281, 288, 570
DpnII GATC 5 cut(s) 138, 181, 279, 286, 568
EaeI YGGCCR 1 cut(s) 968
EagI CGGCCG 1 cut(s) 968
Eam1104I CTCTTC 3 cut(s) 44, 49, 232
EarI CTCTTC 3 cut(s) 44, 49, 232
EciI GGCGGA 1 cut(s) 639
EclXI CGGCCG 1 cut(s) 968
Eco105I TACGTA 1 cut(s) 480
Eco130I CCWWGG 2 cut(s) 387, 692
Eco24I GRGCYC 1 cut(s) 838
Eco47I GGWCC 2 cut(s) 559, 589
Eco52I CGGCCG 1 cut(s) 968
Eco57I CTGAAG 1 cut(s) 1004
EcoRII CCWGG 2 cut(s) 540, 844
EcoT14I CCWWGG 2 cut(s) 387, 692
EcoT38I GRGCYC 1 cut(s) 838
ErhI CCWWGG 2 cut(s) 387, 692
Esp3I CGTCTC 1 cut(s) 793
FaeI CATG 5 cut(s) 57, 199, 430, 772, 1019
FalI AAGNNNNNCTT 2 cut(s) 620, 652
FaqI GGGAC 2 cut(s) 602, 873
FatI CATG 5 cut(s) 53, 195, 426, 768, 1015
FblI GTMKAC 2 cut(s) 488, 919
Fnu4HI GCNGC 6 cut(s) 604, 607, 610, 842, 882, 926
FokI GGATG 2 cut(s) 187, 944
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 6 cut(s) 604, 607, 610, 842, 882, 926
GluI GCNGC 6 cut(s) 604, 607, 610, 842, 882, 926
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 652
HaeIII GGCC 2 cut(s) 116, 970
HapII CCGG 9 cut(s) 155, 174, 333, 521, 577, 596, 913, 929, 967
HgaI GACGC 2 cut(s) 810, 905
Hin1I GRCGYC 1 cut(s) 916
Hin1II CATG 5 cut(s) 57, 199, 430, 772, 1019
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 4 cut(s) 687, 780, 849, 861
HpaII CCGG 9 cut(s) 155, 174, 333, 521, 577, 596, 913, 929, 967
HphI GGTGA 4 cut(s) 232, 322, 484, 530
Hpy166II GTNNAC 4 cut(s) 489, 531, 701, 920
Hpy188I TCNGA 6 cut(s) 14, 138, 186, 324, 559, 961
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 4 cut(s) 489, 531, 701, 920
Hpy99I CGWCG 3 cut(s) 493, 559, 569
HpyAV CCTTC 2 cut(s) 44, 967
HpyCH4III ACNGT 3 cut(s) 244, 448, 718
HpyCH4IV ACGT 5 cut(s) 371, 479, 491, 509, 533
HpyCH4V TGCA 5 cut(s) 87, 426, 701, 768, 1026
HpyF10VI GCNNNNNNNGC 7 cut(s) 108, 609, 707, 774, 878, 922, 1008
HpyF3I CTNAG 3 cut(s) 11, 76, 902
HpySE526I ACGT 5 cut(s) 371, 479, 491, 509, 533
Hsp92I GRCGYC 1 cut(s) 916
Hsp92II CATG 5 cut(s) 57, 199, 430, 772, 1019
KroI GCCGGC 1 cut(s) 912
KroNI GCCGGC 1 cut(s) 914
Kzo9I GATC 5 cut(s) 138, 181, 279, 286, 568
LmnI GCTCC 4 cut(s) 318, 389, 671, 841
Lsp1109I GCAGC 4 cut(s) 596, 618, 853, 937
LweI GCATC 1 cut(s) 1035
MaeII ACGT 5 cut(s) 371, 479, 491, 509, 533
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 140, 183, 281, 288, 570
MboI GATC 5 cut(s) 138, 181, 279, 286, 568
MboII GAAGA 7 cut(s) 16, 31, 36, 212, 219, 374, 997
MfeI CAATTG 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 703, 838
MluCI AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
MlyI GAGTC 2 cut(s) 774, 855
MmeI TCCRAC 4 cut(s) 462, 537, 706, 984
MroNI GCCGGC 1 cut(s) 912
MroXI GAANNNNTTC 1 cut(s) 511
MseI TTAA 6 cut(s) 60, 378, 675, 791, 822, 1041
MslI CAYNNNNRTG 1 cut(s) 52
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 9 cut(s) 155, 174, 333, 521, 577, 596, 913, 929, 967
MspR9I CCNGG 4 cut(s) 174, 542, 597, 846
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 2 cut(s) 1004, 1054
MvaI CCWGG 2 cut(s) 542, 846
MwoI GCNNNNNNNGC 7 cut(s) 108, 609, 707, 774, 878, 922, 1008
NaeI GCCGGC 1 cut(s) 914
NciI CCSGG 2 cut(s) 174, 597
NdeII GATC 5 cut(s) 138, 181, 279, 286, 568
NgoMIV GCCGGC 1 cut(s) 912
NlaIII CATG 5 cut(s) 57, 199, 430, 772, 1019
NlaIV GGNNCC 4 cut(s) 314, 436, 590, 837
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 772
PaqCI CACCTGC 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PctI GAATGC 2 cut(s) 1004, 1054
PdiI GCCGGC 1 cut(s) 914
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 687, 849
PkrI GCNGC 6 cut(s) 605, 608, 611, 843, 883, 927
PleI GAGTC 2 cut(s) 774, 855
PpsI GAGTC 2 cut(s) 774, 855
Ppu21I YACGTR 1 cut(s) 480
Psp1406I AACGTT 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 540, 844
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 2 cut(s) 540, 844
PspN4I GGNNCC 4 cut(s) 314, 436, 590, 837
PspPI GGNCC 2 cut(s) 559, 589
PvuII CAGCTG 1 cut(s) 609
RsaI GTAC 3 cut(s) 73, 404, 482
RsaNI GTAC 3 cut(s) 72, 403, 481
RseI CAYNNNNRTG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 487
SaqAI TTAA 6 cut(s) 60, 378, 675, 791, 822, 1041
SatI GCNGC 6 cut(s) 604, 607, 610, 842, 882, 926
Sau3AI GATC 5 cut(s) 138, 181, 279, 286, 568
Sau96I GGNCC 2 cut(s) 559, 589
SchI GAGTC 2 cut(s) 774, 855
ScrFI CCNGG 4 cut(s) 174, 542, 597, 846
SduI GDGCHC 2 cut(s) 703, 838
SfaNI GCATC 1 cut(s) 1035
SinI GGWCC 2 cut(s) 559, 589
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SnaBI TACGTA 1 cut(s) 480
Sse9I AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
SsiI CCGC 6 cut(s) 495, 603, 624, 839, 881, 923
StyD4I CCNGG 4 cut(s) 172, 540, 595, 844
StyI CCWWGG 2 cut(s) 387, 692
TaaI ACNGT 3 cut(s) 244, 448, 718
TaiI ACGT 5 cut(s) 374, 482, 494, 512, 536
TaqI TCGA 7 cut(s) 141, 488, 525, 564, 864, 947, 1029
TaqII GACCGA 1 cut(s) 606
TasI AATT 8 cut(s) 460, 646, 738, 818, 823, 828, 1021, 1043
TatI WGTACW 1 cut(s) 402
TauI GCSGC 2 cut(s) 606, 884
TfiI GAWTC 2 cut(s) 687, 849
Tru1I TTAA 6 cut(s) 60, 378, 675, 791, 822, 1041
Tru9I TTAA 6 cut(s) 60, 378, 675, 791, 822, 1041
TscAI CASTG 3 cut(s) 216, 723, 910
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 4 cut(s) 606, 609, 841, 925
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 17, 36, 1047
TspGWI ACGGA 2 cut(s) 113, 330
TspRI CASTG 3 cut(s) 216, 723, 910
VneI GTGCAC 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 559, 589
XapI RAATTY 1 cut(s) 818
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 488, 919
XmnI GAANNNNTTC 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.