RchiOBHm_Chr1g0337191

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013938
Physical Location & Seq
Reverse (-)
28875506 .. 28876986
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56510

Sequence Viewer

Length: 1047 bp
ATGGGCTCTGCAAGCAATAATAACCAATATCCATGGCCACCATATGAGACTTTCAAAGACTGTTCTCAAGGAATTTGCAGCATCTACTGCCCACAATGGTGTTACATGATCTTTGCTCCTCCGCCACCTTTCGAGTTCGGCGACAGCTCTGAGGAGAGCGCTGGTATAAACTTCTCTCCTCTTATTATAGCCCTAATAGGCATTGTTGCAAGTGCTTGTATTCTTGTCACGTACTACAGCATCATATCCAAGTATTGCCGGCGACGAGCCGGTGCTTCTGCTAGGGATATTGACAGGGAGGCGAATCCGGCTAGTGAATCGTGGCAGGGCTCTAACACTGGTATGGAAGAGTCACTGATCAAGTCCATCAAGGTTCAGAAGTATAAGAAGAATGATGGGGTGGTAGAAGGCACAGACTGTTCTGTTTGTTTGAGTGAGTTCCAAGAAAATGAAAACTTAAGGCTATTGCCAAAGTGCAACCATGCTTTTCATGTTCCTTGTATTGATGCTTGGCTCAAGTCTCACTCTAGTTGTCCGCTTTGTCGGTCCAATATTGCTGCTCCTGCTATTGCTTTGGTGCCTCAACACCATAATCATCAGCAAGTATTGCCTTCTGTCGAAGAAAATCCACAGCAGGAGCTGATCAATCAATACCAGCATACAAGTGGTGGAACTGTCTTGGTGGTTCAAGATAGGGAAGAAGAGGATTTTCGTGTTCAAAATAATGAGATCCATAGATTAAGGAGATCAGTTTCTTTGATTGAGGTGTTGAATTTACATGATGGTGAAGATGATAAAGACTTGGAAAACCAGATGGAGCATTTCCAGTTTTCTATGGATCAAAGCGAGCAATTTTCGATGCAGAATTTCGAGTTTTCTATGGAAACAGGTTCCTCAAAAGAAGCTGTGTTTGGGGAACAGAATTCTAAATTCAATAATATTAGAAGCGGAGTTTTCAGCTTGGTAAAGGGTCCTCTTGGGCTGAAGAGATCTATTTCAACAGGGAGATTCAAGGTATGCAAAGGAAAAGAACTCTGTTATTCCTAA

Protein Analysis

348

Amino Acids

39.16

Weight (kDa)

5.44

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 139 - 182 2.3e-14 Ring finger domain
zf-RING_11 PF17123 139 - 168 3e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 140 - 181 3.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 140 - 181 6.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 145 - 182 1.1e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03550 AT5G17600
fragaria_vesca FvH4_7g07160
malus_domestica MD02G1239200.v1.1 MD07G1077600.v1.1
prunus_persica Prupe.2G098800_v2.0.a1
pyrus_communis pycom02g20470 pycom07g06130
rosa_chinensis RchiOBHm_Chr1g0337191
rosa_laevigata RLG00000029301
rosa_multiflora Rmu_sc0002160.1_g000007
rosa_roxburghii Rroxscaffold_4G00314890
rosa_rugosa Rorug01G0132000.1
rosa_samantha Rh1AG152600 Rh1BG121600 Rh1DG159900
rosa_wichuraiana Rw1G012480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 577
AciI CCGC 3 cut(s) 122, 536, 948
AclWI GGATC 2 cut(s) 724, 846
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 5 cut(s) 72, 772, 865, 922, 929
AcuI CTGAAG 1 cut(s) 1004
AfaI GTAC 1 cut(s) 233
AfeI AGCGCT 1 cut(s) 160
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 7 cut(s) 55, 689, 719, 772, 934, 999, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AleI CACNNNNGTG 1 cut(s) 97
AluBI AGCT 4 cut(s) 147, 640, 905, 960
AluI AGCT 4 cut(s) 147, 640, 905, 960
Alw26I GTCTC 2 cut(s) 41, 525
AlwI GGATC 2 cut(s) 724, 846
AlwNI CAGNNNCTG 1 cut(s) 640
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 78, 557
ApoI RAATTY 5 cut(s) 72, 772, 865, 922, 929
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 546, 971
AsuHPI GGTGA 1 cut(s) 797
AvaII GGWCC 2 cut(s) 546, 971
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 2 cut(s) 8, 332
BbvI GCAGC 2 cut(s) 90, 544
BccI CCATC 4 cut(s) 374, 389, 776, 808
BclI TGATCA 2 cut(s) 357, 642
BcoDI GTCTC 2 cut(s) 41, 525
BfaI CTAG 3 cut(s) 282, 312, 528
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 457
BglII AGATCT 1 cut(s) 989
BisI GCNGC 2 cut(s) 79, 558
BlsI GCNGC 2 cut(s) 80, 559
Bme18I GGWCC 2 cut(s) 546, 971
BmgT120I GGNCC 2 cut(s) 546, 971
BmiI GGNNCC 3 cut(s) 579, 892, 972
BmsI GCATC 4 cut(s) 90, 249, 496, 849
BpuEI CTTGAG 2 cut(s) 51, 500
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 258, 269
Bse1I ACTGG 2 cut(s) 343, 826
BseDI CCNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 2 cut(s) 343, 826
BseRI GAGGAG 3 cut(s) 108, 167, 168
BseXI GCAGC 2 cut(s) 90, 544
BshFI GGCC 1 cut(s) 37
BshNI GGYRCC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 259, 270, 308
BsmAI GTCTC 2 cut(s) 41, 525
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 8, 332
Bsp143I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 3 cut(s) 122, 536, 948
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 3 cut(s) 579, 892, 972
BspPI GGATC 2 cut(s) 724, 846
BspT107I GGYRCC 1 cut(s) 577
BspTI CTTAAG 1 cut(s) 457
BsrFI RCCGGY 2 cut(s) 258, 269
BsrI ACTGG 2 cut(s) 343, 826
BssAI RCCGGY 2 cut(s) 258, 269
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 62, 419, 676
Bst6I CTCTTC 3 cut(s) 342, 696, 980
BstAFI CTTAAG 1 cut(s) 457
BstAPI GCANNNNNTGC 2 cut(s) 87, 607
BstBAI YACGTR 1 cut(s) 231
BstC8I GCNNGC 3 cut(s) 13, 260, 848
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 32
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 7 cut(s) 111, 360, 645, 732, 749, 841, 992
BstMAI GTCTC 2 cut(s) 41, 525
BstMBI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BstMWI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
BstSFI CTRYAG 1 cut(s) 235
BstV1I GCAGC 2 cut(s) 90, 544
BstX2I RGATCY 2 cut(s) 729, 989
BstYI RGATCY 2 cut(s) 729, 989
BsuRI GGCC 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 336, 353
Cac8I GCNNGC 3 cut(s) 13, 260, 848
CaiI CAGNNNCTG 1 cut(s) 640
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 2 cut(s) 258, 269
Cfr13I GGNCC 2 cut(s) 546, 971
Csp6I GTAC 1 cut(s) 232
CviAII CATG 5 cut(s) 33, 106, 482, 491, 779
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 1 cut(s) 150
DpnI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
DpnII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 342, 696, 980
EarI CTCTTC 3 cut(s) 342, 696, 980
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 8, 332
Eco47I GGWCC 2 cut(s) 546, 971
Eco47III AGCGCT 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 971
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 2 cut(s) 8, 332
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 5 cut(s) 36, 109, 485, 494, 782
FatI CATG 5 cut(s) 32, 105, 481, 490, 778
FauNDI CATATG 1 cut(s) 43
FbaI TGATCA 2 cut(s) 357, 642
Fnu4HI GCNGC 2 cut(s) 79, 558
FriOI GRGCYC 2 cut(s) 8, 332
Fsp4HI GCNGC 2 cut(s) 79, 558
FspBI CTAG 3 cut(s) 282, 312, 528
GlaI GCGC 1 cut(s) 160
GluI GCNGC 2 cut(s) 79, 558
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 37
HapII CCGG 3 cut(s) 259, 270, 308
HhaI GCGC 1 cut(s) 161
Hin1II CATG 5 cut(s) 36, 109, 485, 494, 782
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 4 cut(s) 304, 317, 350, 1008
HpaII CCGG 3 cut(s) 259, 270, 308
HphI GGTGA 1 cut(s) 797
Hpy188I TCNGA 2 cut(s) 151, 378
Hpy188III TCNNGA 1 cut(s) 689
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 401, 621
HpyCH4III ACNGT 3 cut(s) 62, 419, 676
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 6 cut(s) 11, 78, 209, 477, 862, 1020
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 5 cut(s) 36, 109, 485, 494, 782
HspAI GCGC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 357, 642
Kzo9I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
LmnI GCTCC 4 cut(s) 121, 565, 637, 817
Lsp1109I GCAGC 2 cut(s) 90, 544
LweI GCATC 4 cut(s) 90, 249, 496, 849
MaeI CTAG 3 cut(s) 282, 312, 528
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 3 cut(s) 101, 226, 351
MalI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
MboI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
MboII GAAGA 7 cut(s) 359, 400, 632, 710, 713, 800, 997
MflI RGATCY 2 cut(s) 729, 989
MhlI GDGCHC 2 cut(s) 8, 332
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 6 cut(s) 72, 772, 851, 865, 922, 929
MluNI TGGCCA 1 cut(s) 37
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 129, 145, 189, 292, 591, 697, 757, 904, 984
Mox20I TGGCCA 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 458, 740
MslI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
Msp20I TGGCCA 1 cut(s) 37
MspCI CTTAAG 1 cut(s) 457
MspI CCGG 3 cut(s) 259, 270, 308
MwoI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
NaeI GCCGGC 1 cut(s) 260
NcoI CCATGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 43
NdeII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 5 cut(s) 36, 109, 485, 494, 782
NlaIV GGNNCC 3 cut(s) 579, 892, 972
NmuCI GTSAC 2 cut(s) 226, 351
OliI CACNNNNGTG 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 304, 317, 1008
PkrI GCNGC 2 cut(s) 80, 559
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 971
Psp5II RGGWCCY 1 cut(s) 971
PspN4I GGNNCC 3 cut(s) 579, 892, 972
PspPI GGNCC 2 cut(s) 546, 971
PspPPI RGGWCCY 1 cut(s) 971
PstNI CAGNNNCTG 1 cut(s) 640
PsuI RGATCY 2 cut(s) 729, 989
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
SaqAI TTAA 2 cut(s) 458, 740
SatI GCNGC 2 cut(s) 79, 558
Sau3AI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Sau96I GGNCC 2 cut(s) 546, 971
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 2 cut(s) 8, 332
SfaNI GCATC 4 cut(s) 90, 249, 496, 849
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 2 cut(s) 546, 971
SmiMI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
SmlI CTYRAG 3 cut(s) 66, 457, 515
SmoI CTYRAG 3 cut(s) 66, 457, 515
Sse9I AATT 6 cut(s) 72, 772, 851, 865, 922, 929
SsiI CCGC 3 cut(s) 122, 536, 948
SspI AATATT 2 cut(s) 553, 940
SspMI CTAG 3 cut(s) 282, 312, 528
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 3 cut(s) 62, 419, 676
TaiI ACGT 1 cut(s) 233
TaqI TCGA 4 cut(s) 132, 618, 857, 870
TaqII GACCGA 1 cut(s) 534
TasI AATT 6 cut(s) 72, 772, 851, 865, 922, 929
TfiI GAWTC 3 cut(s) 304, 317, 1008
Tru1I TTAA 2 cut(s) 458, 740
Tru9I TTAA 2 cut(s) 458, 740
TscAI CASTG 2 cut(s) 343, 360
TseFI GTSAC 2 cut(s) 226, 351
TseI GCWGC 2 cut(s) 78, 557
Tsp45I GTSAC 2 cut(s) 226, 351
TspDTI ATGAA 2 cut(s) 465, 479
TspRI CASTG 2 cut(s) 343, 360
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 2 cut(s) 546, 971
XapI RAATTY 5 cut(s) 72, 772, 865, 922, 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XspI CTAG 3 cut(s) 282, 312, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.