RLG00000029301

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013938
Physical Location & Seq
Forward (+)
36230549 .. 36231570
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029301

Sequence Viewer

Length: 1022 bp
ATGGGCTCTGCAAGCAATAATAACCAATATCCATGGCCACCATATGAGACTTTCAAAGACTGTTCTCAAGGAATTTGCAGCCTCTACTGCCCGCAATGGTGTTACATGATCTTTGCTCCTCCGCCACCTTTCGAGTTCGGCGACAGCTACGAGGAGAGCTCCGGTATGAACTTCTCTCCTCTTATTATAGCCCTAATAGGCATTGTTGCAAGTGCTTTTATTCTTGTCACGTACTACACCATCATATCCAAGTATTGCCGGCGACGGGCTGGTGCTTCTGCTAGGGATATAGACAGGGAGGCGAATCCGGCTAGTGAATCGTGGCAGGGCTCTAACACTGGTATGGAAGAGTCACTGATCAAGTCCATCAAGGTTCATAAGTATAAGAAGAATGATGGGGTGGTAGAAGGCACAGACTGTTCTGTTTGTTTGAGTGAGTTCGAAGAAAATGAGAGCTTAAGGCTATTGCCAAAGTGCAGCCATGCTTTTCATGTTCCTTGTATTGATGCTTGGCTCAAGTCTCACTCTAGTTGTCCTCTTTGTCGGTCCAATATTGCTGCTTCTGCTATTGCTTTGGTGCCTCAACACCATCATCATCAGCAAGTTAGGCCTTCTGTTCAAGAAAATTCACAGCAGGAGCTGATCAATCAATACCAGCATACAAGTAGTGGAAGTGTCTTGGTGGTTCAAGATAGGGAAGAAGAGGGTTTTCGTGTTCAAAATAATGAGATCCAGAGATTAAGGAGATCGGTTTCTTTGGTTGAGGTGTTGAATTTACATGATGGTGAAGATGATGAGGACTTGGAAAATCAGATGGAGCATTTCCAGTTTTCTATGGATCAAAGCGAGCAATTTTCCATGCAGAATTTCGAGTTTTCTATGGAAACAGGGTCCTCAAAAGAAGCTGTGTTTGGGGAACAGAATTCAAGATTCAATAATATTAGAAGTGGAGTTTTCAGTTTGGTAAAGGGTCCTGTTGGGCTGAAGAGATCTATTTCAACAGGGAGATTTGGGTTCACTAG

Protein Analysis

340

Amino Acids

38.34

Weight (kDa)

5.33

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 139 - 182 1.2e-14 Ring finger domain
zf-RING_11 PF17123 139 - 168 1.6e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 140 - 181 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 140 - 181 4.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 141 - 182 1.1e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 155 - 185 9.2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03550 AT5G17600
fragaria_vesca FvH4_7g07160
malus_domestica MD02G1239200.v1.1 MD07G1077600.v1.1
prunus_persica Prupe.2G098800_v2.0.a1
pyrus_communis pycom02g20470 pycom07g06130
rosa_chinensis RchiOBHm_Chr1g0337191
rosa_laevigata RLG00000029301
rosa_multiflora Rmu_sc0002160.1_g000007
rosa_roxburghii Rroxscaffold_4G00314890
rosa_rugosa Rorug01G0132000.1
rosa_samantha Rh1AG152600 Rh1BG121600 Rh1DG159900
rosa_wichuraiana Rw1G012480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 577
AciI CCGC 2 cut(s) 92, 122
AclWI GGATC 2 cut(s) 724, 846
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 5 cut(s) 72, 625, 772, 865, 922
AcuI CTGAAG 1 cut(s) 1004
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 265
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 8 cut(s) 55, 620, 689, 719, 772, 927, 934, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AluBI AGCT 5 cut(s) 147, 159, 456, 640, 905
AluI AGCT 5 cut(s) 147, 159, 456, 640, 905
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 2 cut(s) 41, 525
AlwI GGATC 2 cut(s) 724, 846
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 2 cut(s) 35, 608
ApeKI GCWGC 3 cut(s) 78, 477, 557
ApoI RAATTY 5 cut(s) 72, 625, 772, 865, 922
AspS9I GGNCC 3 cut(s) 546, 891, 971
AsuHPI GGTGA 1 cut(s) 797
AsuII TTCGAA 1 cut(s) 441
AvaII GGWCC 3 cut(s) 546, 891, 971
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 3 cut(s) 8, 161, 332
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 3 cut(s) 90, 489, 544
BccI CCATC 6 cut(s) 248, 374, 389, 597, 776, 808
BclI TGATCA 2 cut(s) 357, 642
BcoDI GTCTC 2 cut(s) 41, 525
BfaI CTAG 4 cut(s) 282, 312, 528, 1020
BfrI CTTAAG 1 cut(s) 457
BglII AGATCT 1 cut(s) 989
BisI GCNGC 3 cut(s) 79, 478, 558
BlsI GCNGC 3 cut(s) 80, 479, 559
Bme18I GGWCC 3 cut(s) 546, 891, 971
BmgT120I GGNCC 3 cut(s) 546, 891, 971
BmiI GGNNCC 3 cut(s) 579, 892, 972
BmsI GCATC 1 cut(s) 496
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 51, 500
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 32
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 2 cut(s) 343, 826
Bse3DI GCAATG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 32
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 343, 826
BseRI GAGGAG 3 cut(s) 108, 167, 168
BseXI GCAGC 3 cut(s) 90, 489, 544
BsgI GTGCAG 1 cut(s) 496
BshFI GGCC 2 cut(s) 37, 610
BshNI GGYRCC 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 3 cut(s) 162, 259, 308
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 2 cut(s) 41, 525
BsnI GGCC 2 cut(s) 37, 610
Bsp119I TTCGAA 1 cut(s) 441
Bsp1286I GDGCHC 3 cut(s) 8, 161, 332
Bsp143I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 2 cut(s) 92, 122
BspANI GGCC 2 cut(s) 37, 610
BspLI GGNNCC 3 cut(s) 579, 892, 972
BspPI GGATC 2 cut(s) 724, 846
BspT104I TTCGAA 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 577
BspTI CTTAAG 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 2 cut(s) 343, 826
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 62, 419
Bst6I CTCTTC 3 cut(s) 342, 696, 980
BstAFI CTTAAG 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 441
BstC8I GCNNGC 4 cut(s) 13, 92, 260, 848
BstDSI CCRYGG 1 cut(s) 32
BstKTI GATC 7 cut(s) 111, 360, 645, 732, 749, 841, 992
BstMAI GTCTC 2 cut(s) 41, 525
BstMBI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BstMWI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
BstV1I GCAGC 3 cut(s) 90, 489, 544
BstX2I RGATCY 2 cut(s) 729, 989
BstYI RGATCY 2 cut(s) 729, 989
BsuRI GGCC 2 cut(s) 37, 610
BtgI CCRYGG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 336, 353
Cac8I GCNNGC 4 cut(s) 13, 92, 260, 848
CaiI CAGNNNCTG 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 3 cut(s) 546, 891, 971
Csp6I GTAC 1 cut(s) 232
CviAII CATG 6 cut(s) 33, 106, 482, 491, 779, 859
CviQI GTAC 1 cut(s) 232
DpnI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
DpnII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 342, 696, 980
EarI CTCTTC 3 cut(s) 342, 696, 980
EciI GGCGGA 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 32
Eco147I AGGCCT 1 cut(s) 610
Eco24I GRGCYC 3 cut(s) 8, 161, 332
Eco47I GGWCC 3 cut(s) 546, 891, 971
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 1004
EcoICRI GAGCTC 1 cut(s) 159
EcoO109I RGGNCCY 2 cut(s) 891, 971
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 3 cut(s) 8, 161, 332
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 6 cut(s) 36, 109, 485, 494, 782, 862
FatI CATG 6 cut(s) 32, 105, 481, 490, 778, 858
FauI CCCGC 1 cut(s) 99
FauNDI CATATG 1 cut(s) 43
FbaI TGATCA 2 cut(s) 357, 642
Fnu4HI GCNGC 3 cut(s) 79, 478, 558
FriOI GRGCYC 3 cut(s) 8, 161, 332
Fsp4HI GCNGC 3 cut(s) 79, 478, 558
FspBI CTAG 4 cut(s) 282, 312, 528, 1020
GluI GCNGC 3 cut(s) 79, 478, 558
HaeIII GGCC 2 cut(s) 37, 610
HapII CCGG 3 cut(s) 162, 259, 308
Hin1II CATG 6 cut(s) 36, 109, 485, 494, 782, 862
HinfI GANTC 4 cut(s) 304, 317, 350, 930
HpaII CCGG 3 cut(s) 162, 259, 308
HphI GGTGA 1 cut(s) 797
Hpy166II GTNNAC 1 cut(s) 1017
Hpy188I TCNGA 1 cut(s) 813
Hpy188III TCNNGA 4 cut(s) 620, 689, 733, 927
Hpy8I GTNNAC 1 cut(s) 1017
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 401, 621
HpyCH4III ACNGT 2 cut(s) 62, 419
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 5 cut(s) 11, 78, 209, 477, 862
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 6 cut(s) 36, 109, 485, 494, 782, 862
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 357, 642
Kzo9I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
LmnI GCTCC 4 cut(s) 121, 164, 637, 817
Lsp1109I GCAGC 3 cut(s) 90, 489, 544
LweI GCATC 1 cut(s) 496
MaeI CTAG 4 cut(s) 282, 312, 528, 1020
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 3 cut(s) 101, 226, 351
MalI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
MboI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
MboII GAAGA 7 cut(s) 359, 400, 455, 710, 713, 800, 997
MflI RGATCY 2 cut(s) 729, 989
MhlI GDGCHC 3 cut(s) 8, 161, 332
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 6 cut(s) 72, 625, 772, 851, 865, 922
MluNI TGGCCA 1 cut(s) 37
MlyI GAGTC 1 cut(s) 359
Mox20I TGGCCA 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 458, 740
MslI CAYNNNNRTG 2 cut(s) 341, 783
Msp20I TGGCCA 1 cut(s) 37
MspCI CTTAAG 1 cut(s) 457
MspI CCGG 3 cut(s) 162, 259, 308
MwoI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
NaeI GCCGGC 1 cut(s) 260
NcoI CCATGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 43
NdeII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 6 cut(s) 36, 109, 485, 494, 782, 862
NlaIV GGNNCC 3 cut(s) 579, 892, 972
NmuCI GTSAC 2 cut(s) 226, 351
NspV TTCGAA 1 cut(s) 441
PceI AGGCCT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 304, 317, 930
PkrI GCNGC 3 cut(s) 80, 479, 559
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 2 cut(s) 891, 971
Psp124BI GAGCTC 1 cut(s) 161
Psp5II RGGWCCY 2 cut(s) 891, 971
PspN4I GGNNCC 3 cut(s) 579, 892, 972
PspPI GGNCC 3 cut(s) 546, 891, 971
PspPPI RGGWCCY 2 cut(s) 891, 971
PstNI CAGNNNCTG 1 cut(s) 640
PsuI RGATCY 2 cut(s) 729, 989
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 2 cut(s) 341, 783
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 2 cut(s) 458, 740
SatI GCNGC 3 cut(s) 79, 478, 558
Sau3AI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Sau96I GGNCC 3 cut(s) 546, 891, 971
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 3 cut(s) 8, 161, 332
SetI ASST 9 cut(s) 130, 149, 161, 233, 375, 458, 642, 768, 907
SfaNI GCATC 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 441
SinI GGWCC 3 cut(s) 546, 891, 971
SmiMI CAYNNNNRTG 2 cut(s) 341, 783
SmlI CTYRAG 3 cut(s) 66, 457, 515
SmoI CTYRAG 3 cut(s) 66, 457, 515
Sse9I AATT 6 cut(s) 72, 625, 772, 851, 865, 922
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 2 cut(s) 92, 122
SspI AATATT 2 cut(s) 553, 940
SspMI CTAG 4 cut(s) 282, 312, 528, 1020
SstI GAGCTC 1 cut(s) 161
StuI AGGCCT 1 cut(s) 610
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 62, 419
TaiI ACGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 132, 441, 870
TaqII GACCGA 1 cut(s) 534
TasI AATT 6 cut(s) 72, 625, 772, 851, 865, 922
TfiI GAWTC 3 cut(s) 304, 317, 930
Tru1I TTAA 2 cut(s) 458, 740
Tru9I TTAA 2 cut(s) 458, 740
TscAI CASTG 2 cut(s) 343, 360
TseFI GTSAC 2 cut(s) 226, 351
TseI GCWGC 3 cut(s) 78, 477, 557
Tsp45I GTSAC 2 cut(s) 226, 351
TspDTI ATGAA 3 cut(s) 182, 365, 479
TspRI CASTG 2 cut(s) 343, 360
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 3 cut(s) 546, 891, 971
XapI RAATTY 5 cut(s) 72, 625, 772, 865, 922
XspI CTAG 4 cut(s) 282, 312, 528, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.