Rh1DG159900

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0013938
Physical Location & Seq
Reverse (-)
32982827 .. 32987977
5151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG159900.1

Sequence Viewer

Length: 1059 bp
ATGGGCTCTGCAAGCAATAATAACCAATATCCATGGCCACAATATGAGACTTTCAAAGACTGTTCTCAAGGATTTTGCAGCATCTACTGCCCACAATGGTGTTACATGATCTTCGCTCCTCCGCCACCTTTCGAGTTCGGCGACAGCTCCGAGGAGAGCGCCGGTATAAACTTCTCTCCTCTTATTATAGCCCTAATAGGCATTGTTGCAAGTGCTTGTATTCTTGTCACGTACTACAGCATCATATCCAAGTATTGCCGGCGACGACCCGGTGCTTCTGCTAGGGATATTGACAGGGAGGCGTATCCGGCTAGTGAATCGCGGCAGGGCTCTAACACTGGTATGGAAGAGTCACTGATCAAGTCCATCAAGGTTCATAAGTATAAGAAGAATGATGGGGTGGTAGAAGGCACAGACTGTTCTGTTTGTTTGAGTGAGTTCGAAGAAAATGAAAACTTAAGGCTATTGCCAAAGTGCAACCATGCTTTTCATGTTCCTTGTATTGATGCTTGGCTCAAGTCTCACTCTAGTTGTCCGCTTTGTCGGTCCAATATTGCTGCTCCTGCTATTGCTTTGGTGCCTCAACACCATAATCATCAGCAAGTATTGCCTTCTGTCGAAGAAAATCCACAGCAGGAGCTGATCAATCAATACCAGCATACAAGTGGTGGAAGTGTCTTGGTGGTTCAAGATAGGGAAGAAGAGGATTTTCGTGTTCAAAATAATGAGATCCATAGATTAAGGAGATCAGTTTCTTTGATTGAGGTGTTGAATTTACATGATGGTGAAGATGATGAAGACTTGGAAAACCAGATGGAGCATTTCCAGTTTTCTATGGATCAAAGTGAGCAATTTTCGATGCAGAATTTCGAGTTTTCTATGGAAACAGGGTCCTCAAAAGAAGCTGTGTTTGGGGAACAGAATTCTAAATTCAATAATATTAGAAGTGGAGTTTTCAGCTTGGTAAAGGGTCCTCTTGGGCTGAAGAGATCTATTTCAACAGGGAGATTCAAGCTGAACCACAATGAGAGCGTTACAATCTCCAAATCACCTGCATAA

Protein Analysis

352

Amino Acids

39.63

Weight (kDa)

5.34

Isoelectric Point (pI)

58.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 139 - 182 1.3e-14 Ring finger domain
zf-RING_11 PF17123 139 - 168 3e-11 RING-like zinc finger
zf-C3HC4 PF00097 140 - 181 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 140 - 181 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 143 - 182 1.1e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03550 AT5G17600
fragaria_vesca FvH4_7g07160
malus_domestica MD02G1239200.v1.1 MD07G1077600.v1.1
prunus_persica Prupe.2G098800_v2.0.a1
pyrus_communis pycom02g20470 pycom07g06130
rosa_chinensis RchiOBHm_Chr1g0337191
rosa_laevigata RLG00000029301
rosa_multiflora Rmu_sc0002160.1_g000007
rosa_roxburghii Rroxscaffold_4G00314890
rosa_rugosa Rorug01G0132000.1
rosa_samantha Rh1AG152600 Rh1BG121600 Rh1DG159900
rosa_wichuraiana Rw1G012480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 577
AccII CGCG 1 cut(s) 322
AciI CCGC 3 cut(s) 122, 322, 536
AclWI GGATC 2 cut(s) 724, 846
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 4 cut(s) 772, 865, 922, 929
AcuI CTGAAG 1 cut(s) 1004
AfaI GTAC 1 cut(s) 233
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 7 cut(s) 55, 689, 719, 772, 934, 999, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AleI CACNNNNGTG 1 cut(s) 97
AluBI AGCT 5 cut(s) 147, 640, 905, 960, 1015
AluI AGCT 5 cut(s) 147, 640, 905, 960, 1015
Alw26I GTCTC 2 cut(s) 41, 525
AlwI GGATC 2 cut(s) 724, 846
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 78, 557
ApoI RAATTY 4 cut(s) 772, 865, 922, 929
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 3 cut(s) 546, 891, 971
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 797, 1041
AsuII TTCGAA 1 cut(s) 441
AvaII GGWCC 3 cut(s) 546, 891, 971
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 2 cut(s) 8, 332
BbsI GAAGAC 1 cut(s) 804
BbvI GCAGC 2 cut(s) 90, 544
BccI CCATC 4 cut(s) 374, 389, 776, 808
BciVI GTATCC 1 cut(s) 315
BclI TGATCA 2 cut(s) 357, 642
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 2 cut(s) 41, 525
BfaI CTAG 3 cut(s) 282, 312, 528
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 457
BfuI GTATCC 1 cut(s) 315
BglII AGATCT 1 cut(s) 989
BisI GCNGC 3 cut(s) 79, 323, 558
BlsI GCNGC 3 cut(s) 80, 324, 559
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 3 cut(s) 546, 891, 971
BmgT120I GGNCC 3 cut(s) 546, 891, 971
BmiI GGNNCC 3 cut(s) 579, 892, 972
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 4 cut(s) 90, 249, 496, 849
BpiI GAAGAC 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 51, 500
BpuMI CCSGG 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 32, 150
Bse118I RCCGGY 2 cut(s) 161, 258
Bse1I ACTGG 2 cut(s) 343, 826
BseDI CCNNGG 2 cut(s) 32, 150
BseNI ACTGG 2 cut(s) 343, 826
BseRI GAGGAG 3 cut(s) 108, 167, 168
BseXI GCAGC 2 cut(s) 90, 544
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 37
BshNI GGYRCC 1 cut(s) 577
BsiSI CCGG 4 cut(s) 162, 259, 270, 308
BsmAI GTCTC 2 cut(s) 41, 525
BsnI GGCC 1 cut(s) 37
Bsp119I TTCGAA 1 cut(s) 441
Bsp1286I GDGCHC 2 cut(s) 8, 332
Bsp143I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 3 cut(s) 122, 322, 536
BspANI GGCC 1 cut(s) 37
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 579, 892, 972
BspPI GGATC 2 cut(s) 724, 846
BspT104I TTCGAA 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 577
BspTI CTTAAG 1 cut(s) 457
BsrFI RCCGGY 2 cut(s) 161, 258
BsrI ACTGG 2 cut(s) 343, 826
BssAI RCCGGY 2 cut(s) 161, 258
BssECI CCNNGG 2 cut(s) 32, 150
BssMI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 62, 419
Bst6I CTCTTC 3 cut(s) 342, 696, 980
BstAFI CTTAAG 1 cut(s) 457
BstAPI GCANNNNNTGC 2 cut(s) 87, 607
BstBAI YACGTR 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 441
BstC8I GCNNGC 2 cut(s) 13, 260
BstDSI CCRYGG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 7 cut(s) 111, 360, 645, 732, 749, 841, 992
BstMAI GTCTC 2 cut(s) 41, 525
BstMBI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
BstMWI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 2 cut(s) 90, 544
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 2 cut(s) 729, 989
BstYI RGATCY 2 cut(s) 729, 989
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 336, 353
Cac8I GCNNGC 2 cut(s) 13, 260
CaiI CAGNNNCTG 1 cut(s) 640
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 2 cut(s) 161, 258
Cfr13I GGNCC 3 cut(s) 546, 891, 971
Csp6I GTAC 1 cut(s) 232
CviAII CATG 5 cut(s) 33, 106, 482, 491, 779
CviQI GTAC 1 cut(s) 232
DpnI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
DpnII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 3 cut(s) 342, 696, 980
EarI CTCTTC 3 cut(s) 342, 696, 980
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 8, 332
Eco47I GGWCC 3 cut(s) 546, 891, 971
Eco57I CTGAAG 1 cut(s) 1004
EcoO109I RGGNCCY 2 cut(s) 891, 971
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 2 cut(s) 8, 332
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 5 cut(s) 36, 109, 485, 494, 782
FatI CATG 5 cut(s) 32, 105, 481, 490, 778
FbaI TGATCA 2 cut(s) 357, 642
Fnu4HI GCNGC 3 cut(s) 79, 323, 558
FriOI GRGCYC 2 cut(s) 8, 332
Fsp4HI GCNGC 3 cut(s) 79, 323, 558
FspBI CTAG 3 cut(s) 282, 312, 528
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 79, 323, 558
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 37
HapII CCGG 4 cut(s) 162, 259, 270, 308
HhaI GCGC 1 cut(s) 161
Hin1II CATG 5 cut(s) 36, 109, 485, 494, 782
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 3 cut(s) 317, 350, 1008
HpaII CCGG 4 cut(s) 162, 259, 270, 308
HphI GGTGA 2 cut(s) 797, 1041
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 1 cut(s) 689
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 401, 621
HpyCH4III ACNGT 2 cut(s) 62, 419
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 6 cut(s) 11, 78, 209, 477, 862, 1055
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 5 cut(s) 36, 109, 485, 494, 782
HspAI GCGC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 357, 642
Kzo9I GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
LmnI GCTCC 5 cut(s) 121, 152, 565, 637, 817
Lsp1109I GCAGC 2 cut(s) 90, 544
LweI GCATC 4 cut(s) 90, 249, 496, 849
MaeI CTAG 3 cut(s) 282, 312, 528
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 4 cut(s) 101, 226, 351, 1033
MalI GATC 7 cut(s) 110, 359, 644, 731, 748, 840, 991
MboI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
MflI RGATCY 2 cut(s) 729, 989
MhlI GDGCHC 2 cut(s) 8, 332
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 5 cut(s) 772, 851, 865, 922, 929
MluNI TGGCCA 1 cut(s) 37
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 129, 145, 189, 292, 591, 697, 757, 904, 984
Mox20I TGGCCA 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 37
MseI TTAA 2 cut(s) 458, 740
MslI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
Msp20I TGGCCA 1 cut(s) 37
MspCI CTTAAG 1 cut(s) 457
MspI CCGG 4 cut(s) 162, 259, 270, 308
MspR9I CCNGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 5 cut(s) 12, 87, 308, 563, 607
NaeI GCCGGC 1 cut(s) 260
NciI CCSGG 1 cut(s) 270
NcoI CCATGG 1 cut(s) 32
NdeII GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 5 cut(s) 36, 109, 485, 494, 782
NlaIV GGNNCC 3 cut(s) 579, 892, 972
NmuCI GTSAC 2 cut(s) 226, 351
NspV TTCGAA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 317, 1008
PkrI GCNGC 3 cut(s) 80, 324, 559
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 2 cut(s) 891, 971
Psp5II RGGWCCY 2 cut(s) 891, 971
PspN4I GGNNCC 3 cut(s) 579, 892, 972
PspPI GGNCC 3 cut(s) 546, 891, 971
PspPPI RGGWCCY 2 cut(s) 891, 971
PstNI CAGNNNCTG 1 cut(s) 640
PsuI RGATCY 2 cut(s) 729, 989
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
SaqAI TTAA 2 cut(s) 458, 740
SatI GCNGC 3 cut(s) 79, 323, 558
Sau3AI GATC 7 cut(s) 108, 357, 642, 729, 746, 838, 989
Sau96I GGNCC 3 cut(s) 546, 891, 971
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 2 cut(s) 8, 332
SfaNI GCATC 4 cut(s) 90, 249, 496, 849
SfcI CTRYAG 1 cut(s) 235
SfuI TTCGAA 1 cut(s) 441
SinI GGWCC 3 cut(s) 546, 891, 971
SmiMI CAYNNNNRTG 4 cut(s) 97, 341, 663, 783
SmlI CTYRAG 3 cut(s) 66, 457, 515
SmoI CTYRAG 3 cut(s) 66, 457, 515
Sse9I AATT 5 cut(s) 772, 851, 865, 922, 929
SsiI CCGC 3 cut(s) 122, 322, 536
SspI AATATT 2 cut(s) 553, 940
SspMI CTAG 3 cut(s) 282, 312, 528
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 62, 419
TaiI ACGT 1 cut(s) 233
TaqI TCGA 5 cut(s) 132, 441, 618, 857, 870
TaqII GACCGA 1 cut(s) 534
TasI AATT 5 cut(s) 772, 851, 865, 922, 929
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 2 cut(s) 317, 1008
Tru1I TTAA 2 cut(s) 458, 740
Tru9I TTAA 2 cut(s) 458, 740
TscAI CASTG 2 cut(s) 343, 360
TseFI GTSAC 2 cut(s) 226, 351
TseI GCWGC 2 cut(s) 78, 557
Tsp45I GTSAC 2 cut(s) 226, 351
TspDTI ATGAA 4 cut(s) 365, 465, 479, 810
TspRI CASTG 2 cut(s) 343, 360
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 3 cut(s) 546, 891, 971
XapI RAATTY 4 cut(s) 772, 865, 922, 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XspI CTAG 3 cut(s) 282, 312, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.