RchiOBHm_Chr1g0337671

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016973
Physical Location & Seq
Reverse (-)
29596527 .. 29601958
5432 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56556

Sequence Viewer

Length: 969 bp
ATGTACAACGCAGAGAAGAGGGGCAAGAGACAGTTCATGATCAGGCCTTCGTCCAAGGTCATCATCAAGTTCCTTATCGTTATGCAGAAGCACGGTTATATTGGAGAGTTTGAGTATGTTGATGACCACAGATCTGGAAAGATTGTGGTTGAACTCAATGGGAGGCTGAACAAGTGTGGGGTTATCAGTCCTCGCTTTGATGTTGGTGTCAAGGAGATTGAAGGCTGGACGGCTAGACTTCTTCCGTCAAGACAGGCAAGTCTACTTTCATCGATACAATTTGGTCCTCAAGATGGATGGCTTTCGTCATTTTACTCATGCTTGATAAAAAAGTATGACAAAGAAAATGTTGTAGAAGCAAACTTTGGAGAACTTGAAAAAGAGAGATGTAACAGTAACACTTCCGATCTTTCGTTCATTCACACTCTGAAAACCAACACTGACCTTCTGGTGTGCAAAGCAGAATACTACCATCAGTGTGGTGAATATCAAAAATGCTTTGAACTAACCTCTTTCCTACTTGAAAAAGACACTTTACATCTCAAAAGTACATTAGTGCATCTAGCAGCTGCGATGGAACTTGGGAATTCAAATGAGCTCTATCTTATGGCATGCAATTTAGTGAAGGACTATCTTCAAAAGGCTTTGTCATGGTTTTCTGTTGGCTGTTACTACTATTGTATAAAAAAGTATGATCAGTCACGCCGTTATTTCAGTAAGGCTACCAATGTCGATGGAAACTTTCCACCTGTATGGATAGGATATGGGAATGCTTATTCTGCTCAAGATGAGGGAGATCAAGCTATGTCAATTTATCGCACTGCTGCTCGTTTGTTTCCAGGGTGTCATTTACCAACTTTGTACATTGGAAAGGAGTACATGCGAACCCACAGCTACAAACTTGCTGAGCAGGGTACTCTTAATGATGGAAGAGTAATTGCCGTGAAGCAATTGTCTGCAGCATCCTAG

Protein Analysis

322

Amino Acids

36.78

Weight (kDa)

8.65

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 2 - 73 2.9e-07 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 262
AcsI RAATTY 1 cut(s) 586
AfaI GTAC 5 cut(s) 5, 550, 863, 878, 916
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 7 cut(s) 152, 221, 377, 503, 524, 591, 638
AjnI CCWGG 1 cut(s) 838
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AluBI AGCT 4 cut(s) 569, 598, 803, 894
AluI AGCT 4 cut(s) 569, 598, 803, 894
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 1 cut(s) 22
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 4 cut(s) 566, 569, 824, 959
ApoI RAATTY 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 494
AvaII GGWCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 3 cut(s) 556, 578, 811
BccI CCATC 6 cut(s) 287, 291, 480, 568, 728, 920
BceAI ACGGC 3 cut(s) 246, 690, 926
BciT130I CCWGG 1 cut(s) 840
BclI TGATCA 2 cut(s) 39, 694
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 3 cut(s) 234, 563, 967
BfmI CTRYAG 1 cut(s) 957
BglII AGATCT 1 cut(s) 131
BisI GCNGC 4 cut(s) 567, 570, 825, 960
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 4 cut(s) 568, 571, 826, 961
Bme1390I CCNGG 1 cut(s) 840
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 840
BmsI GCATC 1 cut(s) 568
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 2 cut(s) 273, 768
Bsa29I ATCGAT 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 54, 839
Bsc4I CCNNNNNNNGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 272
BseDI CCNNGG 2 cut(s) 54, 839
BseGI GGATG 2 cut(s) 302, 962
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 897
BseXI GCAGC 3 cut(s) 556, 578, 811
BshFI GGCC 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 600
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 600
Bsp1407I TGTACA 2 cut(s) 3, 861
Bsp143I GATC 5 cut(s) 39, 131, 406, 694, 796
Bsp1720I GCTNAGC 1 cut(s) 906
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 1 cut(s) 898
BspDI ATCGAT 1 cut(s) 272
BspHI TCATGA 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 961
BsrGI TGTACA 2 cut(s) 3, 861
BssECI CCNNGG 2 cut(s) 54, 839
BssMI GATC 5 cut(s) 39, 131, 406, 694, 796
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 3 cut(s) 33, 95, 395
Bst6I CTCTTC 2 cut(s) 11, 925
BstAUI TGTACA 2 cut(s) 3, 861
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 1 cut(s) 906
BstF5I GGATG 2 cut(s) 302, 962
BstKTI GATC 5 cut(s) 42, 134, 409, 697, 799
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 5 cut(s) 39, 131, 406, 694, 796
BstMWI GCNNNNNNNGC 1 cut(s) 779
BstNI CCWGG 1 cut(s) 840
BstNSI RCATGY 2 cut(s) 615, 883
BstSCI CCNGG 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 957
BstV1I GCAGC 3 cut(s) 556, 578, 811
BstX2I RGATCY 1 cut(s) 131
BstXI CCANNNNNNTGG 3 cut(s) 134, 479, 753
BstYI RGATCY 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 272
BsuRI GGCC 1 cut(s) 46
BsuTUI ATCGAT 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 587
BtsCI GGATG 2 cut(s) 302, 962
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 3 cut(s) 438, 482, 819
Cac8I GCNNGC 1 cut(s) 613
CciI TCATGA 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 272
Csp6I GTAC 5 cut(s) 4, 549, 862, 877, 915
CviAII CATG 5 cut(s) 37, 318, 612, 651, 880
CviQI GTAC 5 cut(s) 4, 549, 862, 877, 915
DdeI CTNAG 1 cut(s) 906
DpnI GATC 5 cut(s) 41, 133, 408, 696, 798
DpnII GATC 5 cut(s) 39, 131, 406, 694, 796
DrdI GACNNNNNNGTC 1 cut(s) 258
DseDI GACNNNNNNGTC 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 11, 925
EarI CTCTTC 2 cut(s) 11, 925
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 284
Eco53kI GAGCTC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 5 cut(s) 40, 321, 615, 654, 883
FatI CATG 5 cut(s) 36, 317, 611, 650, 879
FbaI TGATCA 2 cut(s) 39, 694
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 567, 570, 825, 960
FokI GGATG 2 cut(s) 309, 949
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 4 cut(s) 567, 570, 825, 960
FspBI CTAG 3 cut(s) 234, 563, 967
GluI GCNGC 4 cut(s) 567, 570, 825, 960
HaeIII GGCC 1 cut(s) 46
Hin1II CATG 5 cut(s) 40, 321, 615, 654, 883
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 406, 429
Hpy188III TCNNGA 5 cut(s) 37, 135, 249, 290, 785
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 4 cut(s) 57, 215, 455, 619
HpyCH4III ACNGT 3 cut(s) 33, 95, 395
HpyCH4V TGCA 5 cut(s) 85, 456, 559, 615, 959
HpyF10VI GCNNNNNNNGC 1 cut(s) 779
HpyF3I CTNAG 1 cut(s) 906
Hsp92II CATG 5 cut(s) 40, 321, 615, 654, 883
Ksp22I TGATCA 2 cut(s) 39, 694
Kzo9I GATC 5 cut(s) 39, 131, 406, 694, 796
LpnPI CCDG 9 cut(s) 28, 120, 211, 239, 434, 762, 825, 852, 896
Lsp1109I GCAGC 3 cut(s) 556, 578, 811
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 234, 563, 967
MaeIII GTNAC 4 cut(s) 389, 395, 668, 699
MalI GATC 5 cut(s) 41, 133, 408, 696, 798
MboI GATC 5 cut(s) 39, 131, 406, 694, 796
MboII GAAGA 4 cut(s) 28, 233, 626, 942
MfeI CAATTG 1 cut(s) 950
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 6 cut(s) 278, 586, 616, 810, 936, 950
MnlI CCTC 6 cut(s) 12, 156, 201, 297, 520, 784
MseI TTAA 1 cut(s) 921
MslI CAYNNNNRTG 2 cut(s) 477, 751
MspA1I CMGCKG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 840
MunI CAATTG 1 cut(s) 950
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 1 cut(s) 779
NdeII GATC 5 cut(s) 39, 131, 406, 694, 796
NlaIII CATG 5 cut(s) 40, 321, 615, 654, 883
NmuCI GTSAC 1 cut(s) 699
NspI RCATGY 2 cut(s) 615, 883
PaeI GCATGC 1 cut(s) 615
PagI TCATGA 1 cut(s) 36
PceI AGGCCT 1 cut(s) 46
PctI GAATGC 1 cut(s) 775
PkrI GCNGC 4 cut(s) 568, 571, 826, 961
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 838
PspGI CCWGG 1 cut(s) 838
PspPI GGNCC 1 cut(s) 284
PstI CTGCAG 1 cut(s) 961
PsuI RGATCY 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 5 cut(s) 5, 550, 863, 878, 916
RsaNI GTAC 5 cut(s) 4, 549, 862, 877, 915
RseI CAYNNNNRTG 2 cut(s) 477, 751
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 1 cut(s) 921
SatI GCNGC 4 cut(s) 567, 570, 825, 960
Sau3AI GATC 5 cut(s) 39, 131, 406, 694, 796
Sau96I GGNCC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 840
SduI GDGCHC 1 cut(s) 600
SetI ASST 8 cut(s) 60, 447, 512, 571, 600, 751, 805, 896
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 957
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 2 cut(s) 477, 751
SmlI CTYRAG 2 cut(s) 288, 783
SmoI CTYRAG 2 cut(s) 288, 783
SphI GCATGC 1 cut(s) 615
Sse9I AATT 6 cut(s) 278, 586, 616, 810, 936, 950
SseBI AGGCCT 1 cut(s) 46
SspMI CTAG 3 cut(s) 234, 563, 967
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 1 cut(s) 46
StyD4I CCNGG 1 cut(s) 838
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 3 cut(s) 33, 95, 395
TaqI TCGA 2 cut(s) 272, 732
TasI AATT 6 cut(s) 278, 586, 616, 810, 936, 950
TatI WGTACW 4 cut(s) 3, 548, 861, 876
Tru1I TTAA 1 cut(s) 921
Tru9I TTAA 1 cut(s) 921
TscAI CASTG 3 cut(s) 445, 482, 826
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 4 cut(s) 566, 569, 824, 959
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 3 cut(s) 25, 258, 406
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 3 cut(s) 445, 482, 826
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 1 cut(s) 586
XceI RCATGY 2 cut(s) 615, 883
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 3 cut(s) 234, 563, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.