Rh1AG081800

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016973
Physical Location & Seq
Reverse (-)
14063708 .. 14093592
29885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG081800.1

Sequence Viewer

Length: 1056 bp
ATGCGCGAGGACATGGAATGCTTCCTGCATGCTAGGGCACACGAAATTGTGTATGGAGGACTGATGCTACTTACTTTTCCAGGCAGCCCTGACGGCTCTCCTTATTCTCATTCTCTTGTGAGCTTGCAACTTCTTGGTTCTTGCCTCATGGACTTGGTTAGAATAAGGCTTGACTTTTCAAAGGAGGCCTCTTGCAATTGTTGCTGTCTTTCTGTAACTTTAGATTGGTGTTTCTGGACCGTTGCATTGACTTTGTTCAAAACTCTTGCAATATCATTCTCGACGATGACACTCTTCACCGGAATCACTCCTTTGTATGGTTCTAGCCTTGTATATGCAGAGGCAAGTCTACTTTCATCGATACAATTTGGTCCTCAAGATGGATGGCTTTCGTCATTTTACTCATGCTTGATAAAAAAGTATGACAAAGAAAATGTTGTAGAAGCAAACTTTGGAGAACTTGAAAAAGAGAGATGTAACAGTAACACTTCCGATCTTTCGTTCATTCACACTCTGAAAACCAACACTGACCTTCTGGTGTGCAAAGCAGAATACTACCATCAGTGTGGTGAATATCAAAAATGCTTTGAACTTACCTCTTTCCTACTTGAAAAAGACACTTTCCATCTCAAAAGTACATTAGTGCATCTAGCAGCTGCGATGGAACTTGGGAATTCAAATGAGCTCTATCTTATGGCATGCAATTTAGTGAAGGACTATCCTCAAAAGGCTTTGTCATGGTTTTCTGTTGGCTGTTACTACTATTGTATAAAAAAGTATGATCAGTCACGCCGTTATTTCAGTAAGGCTACCAATGTCGACGGAAACTTTCCACCTGCATGGATAGGATATGGGAATGCTTATTCTGCTCAAGATGAGGGAGATCAAGCTATGTCAATTTATCGCACTACTGCTCGTTTGTTTCCAGGGTGTCATTTACCAACTTTGTACATTGGAATCGAGTACATGCGAACCCACAGCTACAAACTTGTTGAGCAGGGTACTCTTAATGATGGAAGAGTAATTGCCGTGAAGCAATTGTCTGCAGCATCCTAG

Protein Analysis

351

Amino Acids

39.6

Weight (kDa)

5.86

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 2 - 54 3.4e-08 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccI GTMKAC 2 cut(s) 349, 819
AccII CGCG 1 cut(s) 6
AcsI RAATTY 1 cut(s) 673
AfaI GTAC 4 cut(s) 637, 950, 965, 1003
AfiI CCNNNNNNNGG 2 cut(s) 317, 380
AgsI TTSAA 6 cut(s) 180, 259, 464, 590, 611, 678
AjnI CCWGG 2 cut(s) 79, 925
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 5 cut(s) 123, 656, 685, 890, 981
AluI AGCT 5 cut(s) 123, 656, 685, 890, 981
Alw21I GWGCWC 1 cut(s) 687
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 4 cut(s) 84, 653, 656, 1046
ApoI RAATTY 1 cut(s) 673
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 2 cut(s) 237, 371
AsuHPI GGTGA 2 cut(s) 289, 581
AvaII GGWCC 2 cut(s) 237, 371
BaeGI GKGCMC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 687
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 3 cut(s) 96, 643, 665
BccI CCATC 6 cut(s) 374, 378, 567, 633, 655, 1007
BceAI ACGGC 3 cut(s) 109, 777, 1013
BciT130I CCWGG 2 cut(s) 81, 927
BclI TGATCA 1 cut(s) 781
BfaI CTAG 4 cut(s) 33, 324, 650, 1054
BfmI CTRYAG 1 cut(s) 1044
BfuAI ACCTGC 1 cut(s) 844
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 4 cut(s) 85, 654, 657, 1047
BlsI GCNGC 4 cut(s) 86, 655, 658, 1048
Bme1390I CCNGG 2 cut(s) 81, 927
Bme18I GGWCC 2 cut(s) 237, 371
BmgT120I GGNCC 2 cut(s) 237, 371
BmrFI CCNGG 2 cut(s) 81, 927
BmsI GCATC 2 cut(s) 54, 655
BpuEI CTTGAG 2 cut(s) 360, 855
Bsa29I ATCGAT 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 926
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 380
BseBI CCWGG 2 cut(s) 81, 927
BseCI ATCGAT 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 926
BseGI GGATG 2 cut(s) 389, 1049
BseLI CCNNNNNNNGG 2 cut(s) 317, 380
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 3 cut(s) 96, 643, 665
Bsh1236I CGCG 1 cut(s) 6
BshFI GGCC 1 cut(s) 188
BshVI ATCGAT 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 2 cut(s) 317, 380
BsmI GAATGC 2 cut(s) 23, 862
BsnI GGCC 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 40, 687
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 3 cut(s) 493, 781, 883
BspANI GGCC 1 cut(s) 188
BspDI ATCGAT 1 cut(s) 359
BspFNI CGCG 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1048
BspMI ACCTGC 1 cut(s) 844
BsrGI TGTACA 1 cut(s) 948
BssECI CCNNGG 1 cut(s) 926
BssMI GATC 3 cut(s) 493, 781, 883
Bst2UI CCWGG 2 cut(s) 81, 927
Bst4CI ACNGT 2 cut(s) 241, 482
Bst6I CTCTTC 2 cut(s) 299, 1012
BstAPI GCANNNNNTGC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 948
BstC8I GCNNGC 3 cut(s) 30, 125, 700
BstF5I GGATG 2 cut(s) 389, 1049
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 3 cut(s) 496, 784, 886
BstMBI GATC 3 cut(s) 493, 781, 883
BstMWI GCNNNNNNNGC 3 cut(s) 93, 201, 866
BstNI CCWGG 2 cut(s) 81, 927
BstNSI RCATGY 3 cut(s) 32, 702, 970
BstSCI CCNGG 2 cut(s) 79, 925
BstSFI CTRYAG 1 cut(s) 1044
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 3 cut(s) 96, 643, 665
BstXI CCANNNNNNTGG 2 cut(s) 566, 840
Bsu15I ATCGAT 1 cut(s) 359
BsuRI GGCC 1 cut(s) 188
BsuTUI ATCGAT 1 cut(s) 359
BtgZI GCGATG 1 cut(s) 674
BtsCI GGATG 2 cut(s) 389, 1049
BtsIMutI CAGTG 2 cut(s) 525, 569
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 30, 125, 700
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 237, 371
ClaI ATCGAT 1 cut(s) 359
Csp6I GTAC 4 cut(s) 636, 949, 964, 1002
CviAII CATG 8 cut(s) 13, 29, 148, 405, 699, 738, 840, 967
CviQI GTAC 4 cut(s) 636, 949, 964, 1002
DpnI GATC 3 cut(s) 495, 783, 885
DpnII GATC 3 cut(s) 493, 781, 883
Eam1104I CTCTTC 2 cut(s) 299, 1012
EarI CTCTTC 2 cut(s) 299, 1012
Ecl136II GAGCTC 1 cut(s) 685
Eco147I AGGCCT 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 687
Eco47I GGWCC 2 cut(s) 237, 371
Eco53kI GAGCTC 1 cut(s) 685
EcoICRI GAGCTC 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 79, 925
EcoT38I GRGCYC 1 cut(s) 687
FaeI CATG 8 cut(s) 16, 32, 151, 408, 702, 741, 843, 970
FatI CATG 8 cut(s) 12, 28, 147, 404, 698, 737, 839, 966
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 2 cut(s) 349, 819
Fnu4HI GCNGC 4 cut(s) 85, 654, 657, 1047
FokI GGATG 2 cut(s) 396, 1036
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 4 cut(s) 85, 654, 657, 1047
FspBI CTAG 4 cut(s) 33, 324, 650, 1054
GlaI GCGC 1 cut(s) 5
GluI GCNGC 4 cut(s) 85, 654, 657, 1047
HaeIII GGCC 1 cut(s) 188
HapII CCGG 1 cut(s) 300
HhaI GCGC 1 cut(s) 6
Hin1II CATG 8 cut(s) 16, 32, 151, 408, 702, 741, 843, 970
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HinfI GANTC 2 cut(s) 303, 957
HpaII CCGG 1 cut(s) 300
HphI GGTGA 2 cut(s) 289, 581
Hpy166II GTNNAC 2 cut(s) 350, 820
Hpy188I TCNGA 2 cut(s) 493, 516
Hpy188III TCNNGA 4 cut(s) 235, 280, 377, 872
Hpy8I GTNNAC 2 cut(s) 350, 820
Hpy99I CGWCG 2 cut(s) 286, 824
HpyAV CCTTC 2 cut(s) 542, 706
HpyCH4III ACNGT 2 cut(s) 241, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 201, 866
Hsp92II CATG 8 cut(s) 16, 32, 151, 408, 702, 741, 843, 970
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 3 cut(s) 493, 781, 883
Lsp1109I GCAGC 3 cut(s) 96, 643, 665
LweI GCATC 2 cut(s) 54, 655
MaeI CTAG 4 cut(s) 33, 324, 650, 1054
MaeIII GTNAC 5 cut(s) 214, 476, 482, 755, 786
MalI GATC 3 cut(s) 495, 783, 885
MboI GATC 3 cut(s) 493, 781, 883
MboII GAAGA 2 cut(s) 286, 1029
MfeI CAATTG 2 cut(s) 196, 1037
MhlI GDGCHC 2 cut(s) 40, 687
MluCI AATT 8 cut(s) 45, 196, 365, 673, 703, 897, 1023, 1037
MnlI CCTC 9 cut(s) 50, 155, 178, 199, 334, 384, 607, 732, 871
MseI TTAA 1 cut(s) 1008
MslI CAYNNNNRTG 2 cut(s) 564, 838
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 81, 927
MunI CAATTG 2 cut(s) 196, 1037
Mva1269I GAATGC 2 cut(s) 23, 862
MvaI CCWGG 2 cut(s) 81, 927
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 3 cut(s) 93, 201, 866
NdeII GATC 3 cut(s) 493, 781, 883
NlaIII CATG 8 cut(s) 16, 32, 151, 408, 702, 741, 843, 970
NmuCI GTSAC 1 cut(s) 786
NspI RCATGY 3 cut(s) 32, 702, 970
PaeI GCATGC 2 cut(s) 32, 702
PaqCI CACCTGC 1 cut(s) 844
PceI AGGCCT 1 cut(s) 188
PctI GAATGC 2 cut(s) 23, 862
PfeI GAWTC 2 cut(s) 303, 957
PkrI GCNGC 4 cut(s) 86, 655, 658, 1048
Psp124BI GAGCTC 1 cut(s) 687
Psp6I CCWGG 2 cut(s) 79, 925
PspGI CCWGG 2 cut(s) 79, 925
PspPI GGNCC 2 cut(s) 237, 371
PstI CTGCAG 1 cut(s) 1048
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 4 cut(s) 637, 950, 965, 1003
RsaNI GTAC 4 cut(s) 636, 949, 964, 1002
RseI CAYNNNNRTG 2 cut(s) 564, 838
SacI GAGCTC 1 cut(s) 687
SalI GTCGAC 1 cut(s) 818
SaqAI TTAA 1 cut(s) 1008
SatI GCNGC 4 cut(s) 85, 654, 657, 1047
Sau3AI GATC 3 cut(s) 493, 781, 883
Sau96I GGNCC 2 cut(s) 237, 371
ScrFI CCNGG 2 cut(s) 81, 927
SduI GDGCHC 2 cut(s) 40, 687
SetI ASST 8 cut(s) 125, 534, 599, 658, 687, 838, 892, 983
SfaNI GCATC 2 cut(s) 54, 655
SfcI CTRYAG 1 cut(s) 1044
SinI GGWCC 2 cut(s) 237, 371
SmiMI CAYNNNNRTG 2 cut(s) 564, 838
SmlI CTYRAG 2 cut(s) 375, 870
SmoI CTYRAG 2 cut(s) 375, 870
SphI GCATGC 2 cut(s) 32, 702
Sse9I AATT 8 cut(s) 45, 196, 365, 673, 703, 897, 1023, 1037
SseBI AGGCCT 1 cut(s) 188
SspMI CTAG 4 cut(s) 33, 324, 650, 1054
SstI GAGCTC 1 cut(s) 687
StuI AGGCCT 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 79, 925
TaaI ACNGT 2 cut(s) 241, 482
TaqI TCGA 4 cut(s) 281, 359, 819, 960
TasI AATT 8 cut(s) 45, 196, 365, 673, 703, 897, 1023, 1037
TatI WGTACW 3 cut(s) 635, 948, 963
TfiI GAWTC 2 cut(s) 303, 957
Tru1I TTAA 1 cut(s) 1008
Tru9I TTAA 1 cut(s) 1008
TscAI CASTG 2 cut(s) 532, 569
TseFI GTSAC 1 cut(s) 786
TseI GCWGC 4 cut(s) 84, 653, 656, 1046
Tsp45I GTSAC 1 cut(s) 786
TspDTI ATGAA 2 cut(s) 345, 493
TspGWI ACGGA 1 cut(s) 837
TspRI CASTG 2 cut(s) 532, 569
VpaK11BI GGWCC 2 cut(s) 237, 371
XapI RAATTY 1 cut(s) 673
XceI RCATGY 3 cut(s) 32, 702, 970
XmiI GTMKAC 2 cut(s) 349, 819
XspI CTAG 4 cut(s) 33, 324, 650, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.