Rorug06G0145400

ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016973
Physical Location & Seq
Forward (+)
20770564 .. 20773527
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0145400.1

Sequence Viewer

Length: 1398 bp
ATGGAGTTGCAGTTTGAGGGAGAAGGACTGCAGCCGGTGAGCCCTCTCGGTCAGTACTTCACTAGCTCTGTGTTATCGGTATCGAATCTTGGTGTTTTGGAAACTGAGGTTCCAATCGATGAGCTTCCAGCTATGTCGTTGCTTCAAAATGTGTTCCTCCCCATCAGCCCACGGTTCTCCTCGATCATGGTTGAGAGAAGGGGAAAGAAGCAGTGGAAAAGGGTTGAAGTGAAGCTTGAAGACCATGTTAACATCCCTATTTTCCCTTCTGGCCTATCGCCTGCATCATACGATAAGTATCTTGATGACTACATATCAAAGTTAGCAATGGAAAGACTTCCACAACACAAACCTCTCTGGGAAATTCACATTATCAAGTACCCAACTACCAATGCAGCCGGCACTGTAATATTCAAGCTTCACCAAGCGCTTGGTGATGGCTTCTCTCTCATGGGTGCTCTTCTCTCTTGTTTACAACGAGCTGACAATCCTTCTCTTCCCCTAACCTTCCCGTCAAGAAGGAGGTCGCAGCCATGGAAGAGTGAAAATTTTGTGACTAAAGTTTTTTCTTCTGTCTTCAACACCATATCGGATTTTTTAAATAGCAACTTGGAGGAAGATGATCAAACACCAATCAGATCGGGGAATGATGGAATTGAGTTCAAGCCAATTGCAGTATCAACTATGACGATTTCCCTTGATAGAATCAAACTAATCAAGAGCAGGCTCGGAGTGACGATAAATGACGTTCTTACCGCGATGATCTTCCTTGGTACTCGACTATACATCCAAGAGACGAACAAAAGTTTAAGCAAAGCAAGAGGCACAGCAATTGTATTGCTCAACACAAGGATGATGGGGAATTACACCTCAGTTCAGGAGATGATCAAACCTAATAGCAAGTTGCCATGGGGGAACCATTTGTCACTGTTGCATGTACCAATACCCAAGTTGTTAACGAATAGTGAAGAACAATTTTCAGATAATGCGCTTGACTTTGTCTGGAAGGCACATAAAATTATAAGGAGCAAAAGACATTCTTTGGGTATTCATCTCACTGCTGGGTTCTTGGAGATTCTCAACAAATTTGGAGGCCATGAGGCAGCAGCAAGATACATCCGTAACACATTGAAGAAGTCGAGCATGATAATTTCCAATATGATTGGACCGGTGGAACAAATGTCTTTGGCAAATCATCCAGTTAAAGGCTTATACTTTTTGGTCTTTGGTTCACCTGAGGGCCTTGATGTAACAATAGTAAGTTATATGGGAAAGGTGAGGATTGCCTTCAAGATGGAAAAGGGTCTTATTGATCCACCAAAGTTCAAGTCATGCATGGAAAAGTCTTTTGAGATGATACTCGAAGCCTCGGAGAAAACTGCTGTGCAGAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S8

Protein Analysis

465

Amino Acids

52.21

Weight (kDa)

9.25

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 71 - 250 2.9e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 304 - 453 5.1e-37 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AccII CGCG 1 cut(s) 758
AciI CCGC 1 cut(s) 756
AclWI GGATC 1 cut(s) 1307
AcsI RAATTY 3 cut(s) 363, 547, 1085
AfaI GTAC 4 cut(s) 56, 380, 775, 939
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 1 cut(s) 1205
AgeI ACCGGT 1 cut(s) 1168
AgsI TTSAA 9 cut(s) 146, 227, 239, 415, 580, 664, 1132, 1291, 1327
AluBI AGCT 6 cut(s) 66, 124, 131, 235, 418, 482
AluI AGCT 6 cut(s) 66, 124, 131, 235, 418, 482
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 1 cut(s) 1307
Aor51HI AGCGCT 1 cut(s) 429
AoxI GGCC 3 cut(s) 271, 1093, 1240
ApeKI GCWGC 5 cut(s) 31, 395, 529, 1103, 1106
ApoI RAATTY 3 cut(s) 363, 547, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 1313, 1345
AsiGI ACCGGT 1 cut(s) 1168
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 2 cut(s) 430, 991
AspS9I GGNCC 2 cut(s) 1166, 1240
AsuHPI GGTGA 5 cut(s) 49, 413, 446, 1224, 1288
AvaII GGWCC 1 cut(s) 1166
AxyI CCTNAGG 1 cut(s) 1236
BaeI ACNNNNGTAYC 2 cut(s) 1105, 1138
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 2 cut(s) 246, 568
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 5 cut(s) 43, 407, 541, 1115, 1118
BccI CCATC 5 cut(s) 170, 431, 644, 850, 1288
BclI TGATCA 2 cut(s) 622, 885
BcoDI GTCTC 1 cut(s) 788
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 431
BisI GCNGC 5 cut(s) 32, 396, 530, 1104, 1107
BlsI GCNGC 5 cut(s) 33, 397, 531, 1105, 1108
BmcAI AGTACT 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 2 cut(s) 1166, 1240
BmiI GGNNCC 2 cut(s) 111, 917
BmsI GCATC 1 cut(s) 293
BpiI GAAGAC 2 cut(s) 246, 568
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 297
BsaJI CCNNGG 5 cut(s) 170, 533, 769, 908, 1368
BsaWI WCCGGW 1 cut(s) 1168
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 1205
Bse118I RCCGGY 3 cut(s) 34, 398, 1168
Bse1I ACTGG 1 cut(s) 1199
Bse21I CCTNAGG 1 cut(s) 1236
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 297
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 5 cut(s) 170, 533, 769, 908, 1368
BseGI GGATG 5 cut(s) 252, 786, 858, 1116, 1195
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 1205
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 3 cut(s) 96, 885, 1227
BseNI ACTGG 1 cut(s) 1199
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 5 cut(s) 43, 407, 541, 1115, 1118
BseYI CCCAGC 1 cut(s) 1061
Bsh1236I CGCG 1 cut(s) 758
BshFI GGCC 3 cut(s) 273, 1095, 1242
BshTI ACCGGT 1 cut(s) 1168
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 3 cut(s) 35, 399, 1169
BslI CCNNNNNNNGG 1 cut(s) 1205
BsmAI GTCTC 1 cut(s) 788
BsmBI CGTCTC 1 cut(s) 788
BsnI GGCC 3 cut(s) 273, 1095, 1242
Bsp1286I GDGCHC 2 cut(s) 44, 460
Bsp143I GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
Bsp19I CCATGG 2 cut(s) 533, 908
BspACI CCGC 1 cut(s) 756
BspANI GGCC 3 cut(s) 273, 1095, 1242
BspCNI CTCAG 3 cut(s) 97, 884, 1228
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 1 cut(s) 758
BspLI GGNNCC 2 cut(s) 111, 917
BspMAI CTGCAG 1 cut(s) 33
BspPI GGATC 1 cut(s) 1307
BspQI GCTCTTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 3 cut(s) 34, 398, 1168
BsrI ACTGG 1 cut(s) 1199
BssAI RCCGGY 3 cut(s) 34, 398, 1168
BssECI CCNNGG 5 cut(s) 170, 533, 769, 908, 1368
BssMI GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
BssT1I CCWWGG 3 cut(s) 533, 769, 908
Bst4CI ACNGT 3 cut(s) 174, 406, 930
Bst6I CTCTTC 3 cut(s) 465, 501, 533
BstC8I GCNNGC 3 cut(s) 282, 400, 725
BstDEI CTNAG 3 cut(s) 105, 871, 1236
BstDSI CCRYGG 3 cut(s) 170, 533, 908
BstF5I GGATG 5 cut(s) 252, 786, 858, 1116, 1195
BstFNI CGCG 1 cut(s) 758
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 430, 991
BstKTI GATC 6 cut(s) 186, 625, 641, 765, 888, 1315
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
BstNSI RCATGY 1 cut(s) 938
BstSFI CTRYAG 1 cut(s) 29
BstUI CGCG 1 cut(s) 758
BstV1I GCAGC 5 cut(s) 43, 407, 541, 1115, 1118
BstV2I GAAGAC 2 cut(s) 246, 568
BstXI CCANNNNNNTGG 1 cut(s) 431
Bsu15I ATCGAT 1 cut(s) 117
Bsu36I CCTNAGG 1 cut(s) 1236
BsuRI GGCC 3 cut(s) 273, 1095, 1242
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 3 cut(s) 170, 533, 908
BtgZI GCGATG 1 cut(s) 773
BtsCI GGATG 5 cut(s) 252, 786, 858, 1116, 1195
BtsI GCAGTG 2 cut(s) 218, 1056
BtsIMutI CAGTG 4 cut(s) 218, 402, 926, 1056
Cac8I GCNNGC 3 cut(s) 282, 400, 725
CfoI GCGC 2 cut(s) 430, 991
Cfr10I RCCGGY 3 cut(s) 34, 398, 1168
Cfr13I GGNCC 2 cut(s) 1166, 1240
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 4 cut(s) 55, 379, 774, 938
CspAI ACCGGT 1 cut(s) 1168
CviQI GTAC 4 cut(s) 55, 379, 774, 938
DdeI CTNAG 3 cut(s) 105, 871, 1236
DpnI GATC 6 cut(s) 185, 624, 640, 764, 887, 1314
DpnII GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
DraI TTTAAA 1 cut(s) 600
Eam1104I CTCTTC 3 cut(s) 465, 501, 533
EarI CTCTTC 3 cut(s) 465, 501, 533
Eco130I CCWWGG 3 cut(s) 533, 769, 908
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 1166
Eco47III AGCGCT 1 cut(s) 429
Eco81I CCTNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1240
EcoT14I CCWWGG 3 cut(s) 533, 769, 908
EcoT22I ATGCAT 1 cut(s) 1337
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 3 cut(s) 533, 769, 908
Esp3I CGTCTC 1 cut(s) 788
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 1024, 1056
FbaI TGATCA 2 cut(s) 622, 885
Fnu4HI GCNGC 5 cut(s) 32, 396, 530, 1104, 1107
FokI GGATG 5 cut(s) 239, 773, 865, 1103, 1182
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 32, 396, 530, 1104, 1107
FspBI CTAG 1 cut(s) 63
GlaI GCGC 2 cut(s) 429, 990
GluI GCNGC 5 cut(s) 32, 396, 530, 1104, 1107
GsaI CCCAGC 1 cut(s) 1065
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 3 cut(s) 273, 1095, 1242
HapII CCGG 3 cut(s) 35, 399, 1169
HhaI GCGC 2 cut(s) 430, 991
Hin6I GCGC 2 cut(s) 428, 989
HinP1I GCGC 2 cut(s) 428, 989
HincII GTYRAC 2 cut(s) 250, 957
HindII GTYRAC 2 cut(s) 250, 957
HindIII AAGCTT 2 cut(s) 233, 416
HinfI GANTC 3 cut(s) 85, 705, 1075
HpaI GTTAAC 2 cut(s) 250, 957
HpaII CCGG 3 cut(s) 35, 399, 1169
HphI GGTGA 5 cut(s) 49, 413, 446, 1224, 1288
Hpy166II GTNNAC 4 cut(s) 250, 473, 957, 1232
Hpy188I TCNGA 5 cut(s) 592, 638, 731, 982, 1372
Hpy188III TCNNGA 6 cut(s) 302, 516, 718, 878, 1003, 1291
Hpy8I GTNNAC 4 cut(s) 250, 473, 957, 1232
HpyAV CCTTC 8 cut(s) 17, 192, 276, 501, 513, 517, 1000, 1297
HpyCH4III ACNGT 3 cut(s) 174, 406, 930
HpyCH4IV ACGT 1 cut(s) 747
HpyCH4V TGCA 8 cut(s) 10, 31, 284, 395, 674, 934, 1335, 1387
HpyF3I CTNAG 3 cut(s) 105, 871, 1236
HpySE526I ACGT 1 cut(s) 747
HspAI GCGC 2 cut(s) 428, 989
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 622, 885
KspAI GTTAAC 2 cut(s) 250, 957
Kzo9I GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 1026
Lsp1109I GCAGC 5 cut(s) 43, 407, 541, 1115, 1118
LweI GCATC 1 cut(s) 293
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 747
MaeIII GTNAC 5 cut(s) 553, 733, 924, 1121, 1249
MalI GATC 6 cut(s) 185, 624, 640, 764, 887, 1314
MboI GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
MfeI CAATTG 2 cut(s) 669, 831
MhlI GDGCHC 2 cut(s) 44, 460
Mph1103I ATGCAT 1 cut(s) 1337
MroNI GCCGGC 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 6 cut(s) 249, 599, 809, 956, 1203, 1396
MslI CAYNNNNRTG 1 cut(s) 851
MspI CCGG 3 cut(s) 35, 399, 1169
MunI CAATTG 2 cut(s) 669, 831
MvnI CGCG 1 cut(s) 758
NaeI GCCGGC 1 cut(s) 400
NcoI CCATGG 2 cut(s) 533, 908
NdeII GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
NgoMIV GCCGGC 1 cut(s) 398
NlaIV GGNNCC 2 cut(s) 111, 917
NmuCI GTSAC 3 cut(s) 553, 733, 924
NsiI ATGCAT 1 cut(s) 1337
NspI RCATGY 1 cut(s) 938
PciSI GCTCTTC 1 cut(s) 465
PdiI GCCGGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 3 cut(s) 85, 705, 1075
PflFI GACNNNGTC 1 cut(s) 998
PinAI ACCGGT 1 cut(s) 1168
PkrI GCNGC 5 cut(s) 33, 397, 531, 1105, 1108
PsiI TTATAA 1 cut(s) 1022
PspFI CCCAGC 1 cut(s) 1061
PspN4I GGNNCC 2 cut(s) 111, 917
PspPI GGNCC 2 cut(s) 1166, 1240
PstI CTGCAG 1 cut(s) 33
PsyI GACNNNGTC 1 cut(s) 998
RsaI GTAC 4 cut(s) 56, 380, 775, 939
RsaNI GTAC 4 cut(s) 55, 379, 774, 938
RseI CAYNNNNRTG 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 6 cut(s) 249, 599, 809, 956, 1203, 1396
SatI GCNGC 5 cut(s) 32, 396, 530, 1104, 1107
Sau3AI GATC 6 cut(s) 183, 622, 638, 762, 885, 1312
Sau96I GGNCC 2 cut(s) 1166, 1240
ScaI AGTACT 1 cut(s) 56
SduI GDGCHC 2 cut(s) 44, 460
SfaNI GCATC 1 cut(s) 293
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 851
SsiI CCGC 1 cut(s) 756
SspI AATATT 1 cut(s) 411
SspMI CTAG 1 cut(s) 63
StyI CCWWGG 3 cut(s) 533, 769, 908
TaaI ACNGT 3 cut(s) 174, 406, 930
TaiI ACGT 1 cut(s) 750
TaqI TCGA 6 cut(s) 83, 117, 182, 778, 1139, 1362
TaqII GACCGA 1 cut(s) 38
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 3 cut(s) 85, 705, 1075
Tru1I TTAA 6 cut(s) 249, 599, 809, 956, 1203, 1396
Tru9I TTAA 6 cut(s) 249, 599, 809, 956, 1203, 1396
TscAI CASTG 4 cut(s) 218, 409, 933, 1063
TseFI GTSAC 3 cut(s) 553, 733, 924
TseI GCWGC 5 cut(s) 31, 395, 529, 1103, 1106
Tsp45I GTSAC 3 cut(s) 553, 733, 924
TspDTI ATGAA 1 cut(s) 1040
TspGWI ACGGA 1 cut(s) 1109
TspRI CASTG 4 cut(s) 218, 409, 933, 1063
Tth111I GACNNNGTC 1 cut(s) 998
VpaK11BI GGWCC 1 cut(s) 1166
XapI RAATTY 3 cut(s) 363, 547, 1085
XceI RCATGY 1 cut(s) 938
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 56
Zsp2I ATGCAT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.