RchiOBHm_Chr2g0091881

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017674
Physical Location & Seq
Reverse (-)
5548471 .. 5550063
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46705

Sequence Viewer

Length: 831 bp
ATGAATACAGTGAGGGACTATAGGGATTTACCGGCAGAATCAATGTTCCTTTATGCTCTAAACCAGAGGTTCTTTAAAATAGCTCGTAGAAAAGATCCACCCTTCGTCTCATGCTCAACTTCCGCGGATGTTCTATTTAATCCCTTGAAGGCGTACATGATGACTTCATCTTGTAAAGAAAAGGGAACAATTCAGGCCCAAGAGTCGATCCTGATTGAGGTTGCCAGGGTACGGCTGCATGGGTTTTCAGAACGAGAAGTGTCTATAGTTCGAGCTTTACTGATGTCAGAGATTGAATCTGCATATTTGGAACGCGATCAAATGCAGTCAACTAGCTTCATTTTCTCCGTAATGAACCTGTTATTGGGATTGAGTATGAGGCTCAACTCCAGAAGACTCTTCTACCTTGTGAATATAACAGCAGCAGAGATATCCAAATATGCTGAGAAGTTACAAACATCATGCAGCTGTGTCATTAAGACAATTGAACCACGAGCTTCTGCAATAGTAGAAGATCTGAAAAATGTTGTATCTAAAATCAGTGCTCTTGAGGAAGAAAGAATCATTTCTCCATGGGATGAAGAACATATTCCAGAAGAAATTGTTAGCACAAAACCTAATCCAGGGAATATTGTGCAGCAATGTGAATATTCAAATATTGGAGCTACTGAATTGATTCTATCAAATGGCATGAGAGTTTGCTGTAAGAGTACAGACTTTCTTGATGATCAGGTACAACTCTTTGACAGCAATGCAATATCTGTGAGTTCAGAACTTATCCAAGGAAACACTGTCTATCTGTCCCTCAGTTCTTTGTACTTGAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

276

Amino Acids

31.19

Weight (kDa)

5.31

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30991 FvH4_5g31000
rosa_chinensis RchiOBHm_Chr2g0091881
rosa_laevigata RLG00000011651
rosa_roxburghii Rroxscaffold_2G00149640
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066900 Rh2BG065900 Rh2CG067300 Rh2DG066000 Rh6DG378500
rosa_wichuraiana Rw2G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 125, 315
AciI CCGC 2 cut(s) 123, 125
AclWI GGATC 2 cut(s) 89, 202
AfaI GTAC 5 cut(s) 155, 231, 712, 735, 818
AfiI CCNNNNNNNGG 4 cut(s) 217, 231, 364, 623
AgsI TTSAA 5 cut(s) 148, 296, 488, 654, 823
AjnI CCWGG 2 cut(s) 224, 622
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 83, 275, 336, 468, 497, 665
AluI AGCT 6 cut(s) 83, 275, 336, 468, 497, 665
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 89, 202
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 4 cut(s) 235, 422, 465, 637
Asp700I GAANNNNTTC 3 cut(s) 565, 588, 675
AspS9I GGNCC 1 cut(s) 196
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 4 cut(s) 222, 434, 477, 649
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 2 cut(s) 226, 624
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 19, 264
BglII AGATCT 1 cut(s) 514
BisI GCNGC 4 cut(s) 236, 423, 466, 638
BlsI GCNGC 4 cut(s) 237, 424, 467, 639
Bme1390I CCNGG 2 cut(s) 226, 624
BmgT120I GGNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 226, 624
BpiI GAAGAC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 373
BpuEI CTTGAG 1 cut(s) 569
BsaJI CCNNGG 5 cut(s) 123, 225, 572, 623, 781
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 231, 364, 623
Bse118I RCCGGY 1 cut(s) 31
Bse3DI GCAATG 2 cut(s) 647, 757
BseBI CCWGG 2 cut(s) 226, 624
BseDI CCNNGG 5 cut(s) 123, 225, 572, 623, 781
BseGI GGATG 2 cut(s) 133, 583
BseLI CCNNNNNNNGG 4 cut(s) 217, 231, 364, 623
BseMI GCAATG 2 cut(s) 647, 757
BseMII CTCAG 2 cut(s) 435, 820
BseXI GCAGC 4 cut(s) 222, 434, 477, 649
BsgI GTGCAG 1 cut(s) 656
Bsh1236I CGCG 2 cut(s) 125, 315
BshFI GGCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 2 cut(s) 29, 787
BslI CCNNNNNNNGG 4 cut(s) 217, 231, 364, 623
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 2 cut(s) 29, 787
BsnI GGCC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 5 cut(s) 94, 207, 316, 514, 727
Bsp19I CCATGG 1 cut(s) 572
BspACI CCGC 2 cut(s) 123, 125
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 436, 819
BspFNI CGCG 2 cut(s) 125, 315
BspPI GGATC 2 cut(s) 89, 202
BsrDI GCAATG 2 cut(s) 647, 757
BsrFI RCCGGY 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 5 cut(s) 123, 225, 572, 623, 781
BssMI GATC 5 cut(s) 94, 207, 316, 514, 727
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 2 cut(s) 572, 781
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 2 cut(s) 226, 624
Bst4CI ACNGT 2 cut(s) 10, 793
Bst6I CTCTTC 1 cut(s) 404
BstDEI CTNAG 2 cut(s) 444, 806
BstDSI CCRYGG 2 cut(s) 123, 572
BstENI CCTNNNNNAGG 2 cut(s) 215, 621
BstF5I GGATG 2 cut(s) 133, 583
BstFNI CGCG 2 cut(s) 125, 315
BstKTI GATC 5 cut(s) 97, 210, 319, 517, 730
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 5 cut(s) 94, 207, 316, 514, 727
BstNI CCWGG 2 cut(s) 226, 624
BstSCI CCNGG 2 cut(s) 224, 622
BstSFI CTRYAG 2 cut(s) 19, 264
BstUI CGCG 2 cut(s) 125, 315
BstV1I GCAGC 4 cut(s) 222, 434, 477, 649
BstV2I GAAGAC 1 cut(s) 400
BstX2I RGATCY 2 cut(s) 94, 514
BstYI RGATCY 2 cut(s) 94, 514
BsuRI GGCC 1 cut(s) 197
BtgI CCRYGG 2 cut(s) 123, 572
BtsCI GGATG 2 cut(s) 133, 583
BtsIMutI CAGTG 3 cut(s) 15, 547, 789
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 196
Cfr42I CCGCGG 1 cut(s) 126
Csp6I GTAC 5 cut(s) 154, 230, 711, 734, 817
CviAII CATG 6 cut(s) 111, 157, 239, 462, 573, 691
CviJI RGCY 9 cut(s) 83, 197, 235, 275, 336, 382, 468, 497, 665
CviKI_1 RGCY 9 cut(s) 83, 197, 235, 275, 336, 382, 468, 497, 665
CviQI GTAC 5 cut(s) 154, 230, 711, 734, 817
DdeI CTNAG 2 cut(s) 444, 806
DpnI GATC 5 cut(s) 96, 209, 318, 516, 729
DpnII GATC 5 cut(s) 94, 207, 316, 514, 727
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Eco130I CCWWGG 2 cut(s) 572, 781
Eco32I GATATC 1 cut(s) 432
EcoNI CCTNNNNNAGG 2 cut(s) 215, 621
EcoRII CCWGG 2 cut(s) 224, 622
EcoRV GATATC 1 cut(s) 432
EcoT14I CCWWGG 2 cut(s) 572, 781
ErhI CCWWGG 2 cut(s) 572, 781
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 6 cut(s) 114, 160, 242, 465, 576, 694
FaqI GGGAC 2 cut(s) 29, 787
FatI CATG 6 cut(s) 110, 156, 238, 461, 572, 690
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 4 cut(s) 236, 423, 466, 638
FokI GGATG 2 cut(s) 140, 590
Fsp4HI GCNGC 4 cut(s) 236, 423, 466, 638
FspBI CTAG 1 cut(s) 333
GluI GCNGC 4 cut(s) 236, 423, 466, 638
GsuI CTGGAG 1 cut(s) 373
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 32
Hin1II CATG 6 cut(s) 114, 160, 242, 465, 576, 694
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 6 cut(s) 38, 203, 296, 396, 561, 676
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 4 cut(s) 250, 289, 519, 772
Hpy188III TCNNGA 5 cut(s) 211, 390, 548, 593, 722
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 2 cut(s) 112, 142
HpyCH4III ACNGT 2 cut(s) 10, 793
HpyCH4V TGCA 7 cut(s) 238, 302, 325, 465, 503, 637, 755
HpyF3I CTNAG 2 cut(s) 444, 806
Hsp92II CATG 6 cut(s) 114, 160, 242, 465, 576, 694
Ksp22I TGATCA 1 cut(s) 727
KspI CCGCGG 1 cut(s) 126
Kzo9I GATC 5 cut(s) 94, 207, 316, 514, 727
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 4 cut(s) 222, 434, 477, 649
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 450
MalI GATC 5 cut(s) 96, 209, 318, 516, 729
MboI GATC 5 cut(s) 94, 207, 316, 514, 727
MboII GAAGA 6 cut(s) 391, 405, 524, 566, 593, 608
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 2 cut(s) 94, 514
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 4 cut(s) 189, 483, 600, 671
MlyI GAGTC 2 cut(s) 212, 390
MnlI CCTC 6 cut(s) 6, 60, 211, 372, 544, 815
MroXI GAANNNNTTC 3 cut(s) 565, 588, 675
MseI TTAA 3 cut(s) 75, 138, 477
MspA1I CMGCKG 2 cut(s) 125, 468
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 226, 624
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 226, 624
MvnI CGCG 2 cut(s) 125, 315
NcoI CCATGG 1 cut(s) 572
NdeII GATC 5 cut(s) 94, 207, 316, 514, 727
NlaIII CATG 6 cut(s) 114, 160, 242, 465, 576, 694
PdmI GAANNNNTTC 3 cut(s) 565, 588, 675
PfeI GAWTC 4 cut(s) 38, 296, 561, 676
PkrI GCNGC 4 cut(s) 237, 424, 467, 639
PleI GAGTC 2 cut(s) 211, 390
PpsI GAGTC 2 cut(s) 211, 390
Psp6I CCWGG 2 cut(s) 224, 622
PspGI CCWGG 2 cut(s) 224, 622
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 2 cut(s) 94, 514
PvuII CAGCTG 1 cut(s) 468
RsaI GTAC 5 cut(s) 155, 231, 712, 735, 818
RsaNI GTAC 5 cut(s) 154, 230, 711, 734, 817
SacII CCGCGG 1 cut(s) 126
SaqAI TTAA 3 cut(s) 75, 138, 477
SatI GCNGC 4 cut(s) 236, 423, 466, 638
Sau3AI GATC 5 cut(s) 94, 207, 316, 514, 727
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 2 cut(s) 212, 390
ScrFI CCNGG 2 cut(s) 226, 624
SduI GDGCHC 1 cut(s) 547
SfcI CTRYAG 2 cut(s) 19, 264
Sfr303I CCGCGG 1 cut(s) 126
SgrBI CCGCGG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 4 cut(s) 189, 483, 600, 671
SsiI CCGC 2 cut(s) 123, 125
SspI AATATT 3 cut(s) 631, 650, 658
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 224, 622
StyI CCWWGG 2 cut(s) 572, 781
TaaI ACNGT 2 cut(s) 10, 793
TaqI TCGA 2 cut(s) 206, 271
TasI AATT 4 cut(s) 189, 483, 600, 671
TatI WGTACW 2 cut(s) 710, 816
TfiI GAWTC 4 cut(s) 38, 296, 561, 676
Tru1I TTAA 3 cut(s) 75, 138, 477
Tru9I TTAA 3 cut(s) 75, 138, 477
TscAI CASTG 3 cut(s) 15, 547, 796
TseI GCWGC 4 cut(s) 235, 422, 465, 637
TspDTI ATGAA 5 cut(s) 17, 156, 328, 368, 594
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 3 cut(s) 15, 547, 796
XagI CCTNNNNNAGG 2 cut(s) 215, 621
XmnI GAANNNNTTC 3 cut(s) 565, 588, 675
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.