Rh2BG065900

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017674
Physical Location & Seq
Reverse (-)
5038430 .. 5039603
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG065900.1

Sequence Viewer

Length: 678 bp
ATGAATACAGTGAGGGACTATAGGGATTTACCGGCAGAATCAATGTTCCTTTATTCTCTAAACCAGAGGTTCTTTAAAATAGCTCGTAGAAAAGATCCACCCTTCGTCTCATGCTCAACTTCCGCAGATGTTCTATTTAATCCCTTGAAGGCGTACATGATGACTTCATCTTGTAAAGAAAAGGGAACAATTCAGGCCCAAGAGTCGATCCTGATTGAGGTTGCCAGGGTACGGCTGCATGGGTTTTCAGAACGAGAAGTGTCTATAGTTCGAGCTTTACTGATGTCAGAGATTGAATCTGCATATTTGGAACGCGATCAAATGCAGTCAACTAGCTTGACTTCTGGTATTGATTTTGAAAATTTATTCACCATTATTTTTCTCTGGCAGCATTTTCTCCGTAATGAACCTGTTATTGGGATTGAGTATGAGGCTCAACTCCAGAAGGCTCTTCTACCTTATATAACAGCAGCAGAGATATCCAAATATGCTGAGAAGTTACAAACATCATGCAGCTGTGTCATTAAGACAATTGAACCACGAGCTTCTGCAATAGTAGAAGATCTGAAAAATGTTGTATCTAAAATCAGTGCTCTTGAGGAAGAAAGAATCATTTCTCCATGGGATGAAGAACATATTCCAGAAGAAATTGTTAGCACAAAACCTAATCCAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

225

Amino Acids

25.69

Weight (kDa)

5.17

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30991 FvH4_5g31000
rosa_chinensis RchiOBHm_Chr2g0091881
rosa_laevigata RLG00000011651
rosa_roxburghii Rroxscaffold_2G00149640
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066900 Rh2BG065900 Rh2CG067300 Rh2DG066000 Rh6DG378500
rosa_wichuraiana Rw2G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 315
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 89, 202
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 2 cut(s) 155, 231
AfiI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
AgsI TTSAA 4 cut(s) 148, 296, 359, 536
AjnI CCWGG 2 cut(s) 224, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 5 cut(s) 83, 275, 336, 516, 545
AluI AGCT 5 cut(s) 83, 275, 336, 516, 545
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 89, 202
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 4 cut(s) 235, 388, 470, 513
ApoI RAATTY 1 cut(s) 361
Asp700I GAANNNNTTC 2 cut(s) 613, 636
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 1 cut(s) 361
BauI CACGAG 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 4 cut(s) 222, 400, 482, 525
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 2 cut(s) 226, 672
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 19, 264
BglII AGATCT 1 cut(s) 562
BisI GCNGC 4 cut(s) 236, 389, 471, 514
BlsI GCNGC 4 cut(s) 237, 390, 472, 515
Bme1390I CCNGG 2 cut(s) 226, 672
BmgT120I GGNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 226, 672
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 225, 620, 671
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
Bse118I RCCGGY 1 cut(s) 31
BseBI CCWGG 2 cut(s) 226, 672
BseDI CCNNGG 3 cut(s) 225, 620, 671
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
BseMII CTCAG 1 cut(s) 483
BseXI GCAGC 4 cut(s) 222, 400, 482, 525
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 4 cut(s) 94, 207, 316, 562
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 1 cut(s) 123
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 1 cut(s) 484
BspFNI CGCG 1 cut(s) 315
BspPI GGATC 2 cut(s) 89, 202
BspQI GCTCTTC 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 3 cut(s) 225, 620, 671
BssMI GATC 4 cut(s) 94, 207, 316, 562
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 620
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 226, 672
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 620
BstENI CCTNNNNNAGG 2 cut(s) 215, 669
BstF5I GGATG 1 cut(s) 631
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 4 cut(s) 97, 210, 319, 565
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 4 cut(s) 94, 207, 316, 562
BstNI CCWGG 2 cut(s) 226, 672
BstSCI CCNGG 2 cut(s) 224, 670
BstSFI CTRYAG 2 cut(s) 19, 264
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 4 cut(s) 222, 400, 482, 525
BstX2I RGATCY 2 cut(s) 94, 562
BstYI RGATCY 2 cut(s) 94, 562
BsuRI GGCC 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 1 cut(s) 631
BtsIMutI CAGTG 2 cut(s) 15, 595
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 154, 230
CviAII CATG 5 cut(s) 111, 157, 239, 510, 621
CviJI RGCY 9 cut(s) 83, 197, 235, 275, 336, 434, 449, 516, 545
CviKI_1 RGCY 9 cut(s) 83, 197, 235, 275, 336, 434, 449, 516, 545
CviQI GTAC 2 cut(s) 154, 230
DdeI CTNAG 1 cut(s) 492
DpnI GATC 4 cut(s) 96, 209, 318, 564
DpnII GATC 4 cut(s) 94, 207, 316, 562
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 1 cut(s) 620
Eco32I GATATC 1 cut(s) 480
EcoNI CCTNNNNNAGG 2 cut(s) 215, 669
EcoRII CCWGG 2 cut(s) 224, 670
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 620
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 5 cut(s) 114, 160, 242, 513, 624
FaqI GGGAC 1 cut(s) 29
FatI CATG 5 cut(s) 110, 156, 238, 509, 620
Fnu4HI GCNGC 4 cut(s) 236, 389, 471, 514
FokI GGATG 1 cut(s) 638
Fsp4HI GCNGC 4 cut(s) 236, 389, 471, 514
FspBI CTAG 1 cut(s) 333
GluI GCNGC 4 cut(s) 236, 389, 471, 514
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 32
Hin1II CATG 5 cut(s) 114, 160, 242, 513, 624
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 4 cut(s) 38, 203, 296, 609
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 3 cut(s) 250, 289, 567
Hpy188III TCNNGA 4 cut(s) 211, 442, 596, 641
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 3 cut(s) 112, 142, 439
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 5 cut(s) 238, 302, 325, 513, 551
HpyF3I CTNAG 1 cut(s) 492
Hsp92II CATG 5 cut(s) 114, 160, 242, 513, 624
Kzo9I GATC 4 cut(s) 94, 207, 316, 562
LguI GCTCTTC 1 cut(s) 456
Lsp1109I GCAGC 4 cut(s) 222, 400, 482, 525
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 498
MalI GATC 4 cut(s) 96, 209, 318, 564
MboI GATC 4 cut(s) 94, 207, 316, 562
MboII GAAGA 5 cut(s) 443, 572, 614, 641, 656
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 2 cut(s) 94, 562
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 4 cut(s) 189, 361, 531, 648
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 5 cut(s) 6, 60, 211, 424, 592
MroXI GAANNNNTTC 2 cut(s) 613, 636
MseI TTAA 3 cut(s) 75, 138, 525
MspA1I CMGCKG 1 cut(s) 516
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 226, 672
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 2 cut(s) 226, 672
MvnI CGCG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 620
NdeII GATC 4 cut(s) 94, 207, 316, 562
NlaIII CATG 5 cut(s) 114, 160, 242, 513, 624
PciSI GCTCTTC 1 cut(s) 456
PdmI GAANNNNTTC 2 cut(s) 613, 636
PfeI GAWTC 3 cut(s) 38, 296, 609
PkrI GCNGC 4 cut(s) 237, 390, 472, 515
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 2 cut(s) 224, 670
PspGI CCWGG 2 cut(s) 224, 670
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 2 cut(s) 94, 562
PvuII CAGCTG 1 cut(s) 516
RsaI GTAC 2 cut(s) 155, 231
RsaNI GTAC 2 cut(s) 154, 230
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 75, 138, 525
SatI GCNGC 4 cut(s) 236, 389, 471, 514
Sau3AI GATC 4 cut(s) 94, 207, 316, 562
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 226, 672
SduI GDGCHC 1 cut(s) 595
SfcI CTRYAG 2 cut(s) 19, 264
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 4 cut(s) 189, 361, 531, 648
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 224, 670
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 2 cut(s) 206, 271
TasI AATT 4 cut(s) 189, 361, 531, 648
TfiI GAWTC 3 cut(s) 38, 296, 609
Tru1I TTAA 3 cut(s) 75, 138, 525
Tru9I TTAA 3 cut(s) 75, 138, 525
TscAI CASTG 2 cut(s) 15, 595
TseI GCWGC 4 cut(s) 235, 388, 470, 513
TspDTI ATGAA 4 cut(s) 17, 156, 420, 642
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 15, 595
XagI CCTNNNNNAGG 2 cut(s) 215, 669
XapI RAATTY 1 cut(s) 361
XmnI GAANNNNTTC 2 cut(s) 613, 636
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.