Rh2AG066900

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017674
Physical Location & Seq
Reverse (-)
5576109 .. 5577490
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG066900.1

Sequence Viewer

Length: 828 bp
ATGAATACAGTGAGGGACTATAGGGATTTACCGGCAGAATCAATGTTCCTTTATGCTCTAAACCAGAGGTTCTTTAAAATAGCTCGTAGAAAAGATCCACCCTTCGTCTCATGCTCAACTTCCGCAGATGTTCTATTTAATCCCTTGAAGGCGTACATGATGACTTCATCTTGTAAAGAAAAGGGAACAATTCAGGTCCAAGAGTCGATCCTGATTGAGGTTGCCAGGGTACGGCTGCATGGGTTTTCAGAACGAGAAGTGTCTATAGTTCGAGCTTTACTGATGTCAGAGATTGAATCTGCATATTTGGAACGCGATCAAATGCAGTCAACTAGCTTGACTTCTGGTATTGATTTTGAAAAGTTATTCACCATTATTTTTCTCTGGCAGCATTTTCTCCGTAATGAACCTGTTATTGGGATTGAGTATGAGGCTCAACTCCAGAAGACTCTTCTACCTTATATAACAGCAGCAAAGATATCCAAATATGCTGAGAAGTTACAAACATCATGCAGCTGTGTCATTAAGACAATTGAACCACGAGCTTCTGCAATAGTAGAAGATCTGAAAAATGTTGTATCTAAAATCAGTGCTCTTGAGGAAGAAAGAATCATTTCTCCATGGGATGAAGAACATATTCCAGAAGAAATTGTTAGCACAAAACCTAATCCAGGGAATATTGTGCAGCAATGTGAGTATTCAAATATTGGAGCTACTGAATTGATTCTATCAAATGGCATGAGAGTTTGCTGTAAGAGTACAGACTTTCTTGATGATCAGTTCTTTGTACTTGAAGTTATGAACGCAAGACAATATTCATCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

275

Amino Acids

31.42

Weight (kDa)

5.22

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30991 FvH4_5g31000
rosa_chinensis RchiOBHm_Chr2g0091881
rosa_laevigata RLG00000011651
rosa_roxburghii Rroxscaffold_2G00149640
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066900 Rh2BG065900 Rh2CG067300 Rh2DG066000 Rh6DG378500
rosa_wichuraiana Rw2G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 315
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 89, 202
AfaI GTAC 4 cut(s) 155, 231, 760, 789
AfiI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
AgsI TTSAA 6 cut(s) 148, 296, 359, 536, 702, 794
AjnI CCWGG 2 cut(s) 224, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 6 cut(s) 83, 275, 336, 516, 545, 713
AluI AGCT 6 cut(s) 83, 275, 336, 516, 545, 713
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 89, 202
ApeKI GCWGC 5 cut(s) 235, 388, 470, 513, 685
Asp700I GAANNNNTTC 3 cut(s) 613, 636, 723
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 1 cut(s) 361
AvaII GGWCC 1 cut(s) 196
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 5 cut(s) 222, 400, 482, 525, 697
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 2 cut(s) 226, 672
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 19, 264
BglII AGATCT 1 cut(s) 562
BisI GCNGC 5 cut(s) 236, 389, 471, 514, 686
BlsI GCNGC 5 cut(s) 237, 390, 472, 515, 687
Bme1390I CCNGG 2 cut(s) 226, 672
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 226, 672
BpiI GAAGAC 1 cut(s) 452
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 225, 620, 671
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
Bse118I RCCGGY 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 695
BseBI CCWGG 2 cut(s) 226, 672
BseDI CCNNGG 3 cut(s) 225, 620, 671
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
BseMI GCAATG 1 cut(s) 695
BseMII CTCAG 1 cut(s) 483
BseXI GCAGC 5 cut(s) 222, 400, 482, 525, 697
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 4 cut(s) 217, 231, 416, 671
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 5 cut(s) 94, 207, 316, 562, 775
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 484
BspFNI CGCG 1 cut(s) 315
BspPI GGATC 2 cut(s) 89, 202
BsrDI GCAATG 1 cut(s) 695
BsrFI RCCGGY 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 3 cut(s) 225, 620, 671
BssMI GATC 5 cut(s) 94, 207, 316, 562, 775
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 620
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 226, 672
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 620
BstENI CCTNNNNNAGG 2 cut(s) 215, 669
BstF5I GGATG 1 cut(s) 631
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 5 cut(s) 97, 210, 319, 565, 778
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 5 cut(s) 94, 207, 316, 562, 775
BstNI CCWGG 2 cut(s) 226, 672
BstSCI CCNGG 2 cut(s) 224, 670
BstSFI CTRYAG 2 cut(s) 19, 264
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 5 cut(s) 222, 400, 482, 525, 697
BstV2I GAAGAC 1 cut(s) 452
BstX2I RGATCY 2 cut(s) 94, 562
BstYI RGATCY 2 cut(s) 94, 562
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 1 cut(s) 631
BtsIMutI CAGTG 2 cut(s) 15, 595
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 4 cut(s) 154, 230, 759, 788
CviAII CATG 6 cut(s) 111, 157, 239, 510, 621, 739
CviJI RGCY 8 cut(s) 83, 235, 275, 336, 434, 516, 545, 713
CviKI_1 RGCY 8 cut(s) 83, 235, 275, 336, 434, 516, 545, 713
CviQI GTAC 4 cut(s) 154, 230, 759, 788
DdeI CTNAG 1 cut(s) 492
DpnI GATC 5 cut(s) 96, 209, 318, 564, 777
DpnII GATC 5 cut(s) 94, 207, 316, 562, 775
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 1 cut(s) 620
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 196
EcoNI CCTNNNNNAGG 2 cut(s) 215, 669
EcoRII CCWGG 2 cut(s) 224, 670
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 620
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 6 cut(s) 114, 160, 242, 513, 624, 742
FaqI GGGAC 1 cut(s) 29
FatI CATG 6 cut(s) 110, 156, 238, 509, 620, 738
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 5 cut(s) 236, 389, 471, 514, 686
FokI GGATG 1 cut(s) 638
Fsp4HI GCNGC 5 cut(s) 236, 389, 471, 514, 686
FspBI CTAG 1 cut(s) 333
GluI GCNGC 5 cut(s) 236, 389, 471, 514, 686
GsuI CTGGAG 1 cut(s) 425
HapII CCGG 1 cut(s) 32
Hin1II CATG 6 cut(s) 114, 160, 242, 513, 624, 742
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 6 cut(s) 38, 203, 296, 448, 609, 724
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 3 cut(s) 250, 289, 567
Hpy188III TCNNGA 5 cut(s) 211, 442, 596, 641, 770
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 2 cut(s) 112, 142
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 6 cut(s) 238, 302, 325, 513, 551, 685
HpyF3I CTNAG 1 cut(s) 492
Hsp92II CATG 6 cut(s) 114, 160, 242, 513, 624, 742
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 5 cut(s) 94, 207, 316, 562, 775
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 5 cut(s) 222, 400, 482, 525, 697
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 498
MalI GATC 5 cut(s) 96, 209, 318, 564, 777
MboI GATC 5 cut(s) 94, 207, 316, 562, 775
MboII GAAGA 6 cut(s) 443, 457, 572, 614, 641, 656
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 2 cut(s) 94, 562
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 4 cut(s) 189, 531, 648, 719
MlyI GAGTC 2 cut(s) 212, 442
MnlI CCTC 5 cut(s) 6, 60, 211, 424, 592
MroXI GAANNNNTTC 3 cut(s) 613, 636, 723
MseI TTAA 3 cut(s) 75, 138, 525
MspA1I CMGCKG 1 cut(s) 516
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 226, 672
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 2 cut(s) 226, 672
MvnI CGCG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 620
NdeII GATC 5 cut(s) 94, 207, 316, 562, 775
NlaIII CATG 6 cut(s) 114, 160, 242, 513, 624, 742
PdmI GAANNNNTTC 3 cut(s) 613, 636, 723
PfeI GAWTC 4 cut(s) 38, 296, 609, 724
PkrI GCNGC 5 cut(s) 237, 390, 472, 515, 687
PleI GAGTC 2 cut(s) 211, 442
PpsI GAGTC 2 cut(s) 211, 442
Psp6I CCWGG 2 cut(s) 224, 670
PspGI CCWGG 2 cut(s) 224, 670
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 2 cut(s) 94, 562
PvuII CAGCTG 1 cut(s) 516
RsaI GTAC 4 cut(s) 155, 231, 760, 789
RsaNI GTAC 4 cut(s) 154, 230, 759, 788
SaqAI TTAA 3 cut(s) 75, 138, 525
SatI GCNGC 5 cut(s) 236, 389, 471, 514, 686
Sau3AI GATC 5 cut(s) 94, 207, 316, 562, 775
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 2 cut(s) 212, 442
ScrFI CCNGG 2 cut(s) 226, 672
SduI GDGCHC 1 cut(s) 595
SfcI CTRYAG 2 cut(s) 19, 264
SinI GGWCC 1 cut(s) 196
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 4 cut(s) 189, 531, 648, 719
SsiI CCGC 1 cut(s) 123
SspI AATATT 3 cut(s) 679, 706, 815
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 224, 670
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 2 cut(s) 206, 271
TasI AATT 4 cut(s) 189, 531, 648, 719
TatI WGTACW 2 cut(s) 758, 787
TfiI GAWTC 4 cut(s) 38, 296, 609, 724
Tru1I TTAA 3 cut(s) 75, 138, 525
Tru9I TTAA 3 cut(s) 75, 138, 525
TscAI CASTG 2 cut(s) 15, 595
TseI GCWGC 5 cut(s) 235, 388, 470, 513, 685
TspDTI ATGAA 6 cut(s) 17, 156, 420, 642, 807, 815
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 15, 595
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 2 cut(s) 215, 669
XmnI GAANNNNTTC 3 cut(s) 613, 636, 723
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.