Rh2DG066000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017674
Physical Location & Seq
Reverse (-)
4973334 .. 4974629
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG066000.1

Sequence Viewer

Length: 768 bp
ATGGGGAACACTACTACCTCTGAGTTTGCTAAGGACATGCTTTATAAGCACTGTTCTGAATGTGAAAAAGAGAGACGGACGTCAACTTATAATTATGAGGGTATAATAGCTGGGTGGCCAACTGGAGGGGTTGGTCAAATGTATGAGTTCTGGGTCATCAAGAACAAACCTGGACATCAAAAAATTATTGTGGATGATTGCTCTACATCGATATTTAAGAACGTTGCCACAGCGGGAAGTGGCAGCCAGCATGCAGCCTCTCATATGGCGACATATGGGGTCTTGAAGGATGAATTAGAAGGCGCCAACTGTTGTCTAGAGGCTATTTTCAGAGCTGGTGGCATTGATCCATCCACCGGAGGAAAATTAGAGGTTGGTTGTCTCAAGGAGGGCACTTATGATGTAATGTTCTCTATGGATGACTTGGACACCTATTCGCACTGTTACACGACAGATTTTGATGAAGTTCTATTTCTATTTGTTCCTAGAAAGGAGCCTGTCACCGCTGATGAGATTAAAAAAGCTTGGGCCGCACATTGCATAGCCGTTACAAATTATTTTGCGTTCCATCACATTTTATTTGTAACCAAGTATGACAAAGAGAGAGCGGAGAAAGACTTTTTAGGCGGAGACGAGCCGGATCTTGGGAAAGTTAATTCGCCTGACGAGTTTCTTGCAGTCCTCCATGAGGTGATAATAGGCGGAACAAAATGTCGTCTAAGCAGAAAGTCAAAGAGAACAATCGATTTGATTCGTCGGATGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.51

Weight (kDa)

5.86

Isoelectric Point (pI)

22.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017674)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30991 FvH4_5g31000
rosa_chinensis RchiOBHm_Chr2g0091881
rosa_laevigata RLG00000011651
rosa_roxburghii Rroxscaffold_2G00149640
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066900 Rh2BG065900 Rh2CG067300 Rh2DG066000 Rh6DG378500
rosa_wichuraiana Rw2G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 45, 90
AatII GACGTC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 302
AccBSI CCGCTC 1 cut(s) 608
AciI CCGC 6 cut(s) 233, 504, 531, 608, 627, 702
AclI AACGTT 1 cut(s) 222
AclWI GGATC 2 cut(s) 341, 648
AcoI YGGCCR 1 cut(s) 116
AcyI GRCGYC 2 cut(s) 80, 303
AfiI CCNNNNNNNGG 4 cut(s) 125, 356, 644, 688
AgsI TTSAA 1 cut(s) 286
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 3 cut(s) 110, 335, 524
AluI AGCT 3 cut(s) 110, 335, 524
Alw26I GTCTC 3 cut(s) 67, 386, 624
AlwI GGATC 2 cut(s) 341, 648
AoxI GGCC 2 cut(s) 116, 528
ApeKI GCWGC 2 cut(s) 243, 254
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 493, 703
BaeGI GKGCMC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 118
BanI GGYRCC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 255, 266
BccI CCATC 2 cut(s) 358, 576
BceAI ACGGC 1 cut(s) 530
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 67, 386, 624
BfaI CTAG 2 cut(s) 317, 486
BfoI RGCGCY 1 cut(s) 306
BisI GCNGC 3 cut(s) 244, 255, 531
BlsI GCNGC 3 cut(s) 245, 256, 532
Bme1390I CCNGG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 2 cut(s) 304, 495
BmrFI CCNGG 1 cut(s) 171
BoxI GACNNNNGTC 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 368
Bsa29I ATCGAT 2 cut(s) 209, 744
BsaHI GRCGYC 2 cut(s) 80, 303
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 356, 644, 688
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 171
BseCI ATCGAT 2 cut(s) 209, 744
BseGI GGATG 5 cut(s) 199, 295, 350, 424, 765
BseLI CCNNNNNNNGG 4 cut(s) 125, 356, 644, 688
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 127
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 2 cut(s) 255, 266
BseYI CCCAGC 1 cut(s) 110
BshFI GGCC 2 cut(s) 118, 530
BshNI GGYRCC 1 cut(s) 302
BshVI ATCGAT 2 cut(s) 209, 744
BsiSI CCGG 2 cut(s) 357, 638
BslI CCNNNNNNNGG 4 cut(s) 125, 356, 644, 688
BsmAI GTCTC 3 cut(s) 67, 386, 624
BsmBI CGTCTC 2 cut(s) 67, 624
BsnI GGCC 2 cut(s) 118, 530
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 346, 640
BspACI CCGC 6 cut(s) 233, 504, 531, 608, 627, 702
BspANI GGCC 2 cut(s) 118, 530
BspCNI CTCAG 1 cut(s) 13
BspDI ATCGAT 2 cut(s) 209, 744
BspLI GGNNCC 2 cut(s) 304, 495
BspPI GGATC 2 cut(s) 341, 648
BspT107I GGYRCC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 1 cut(s) 127
BssMI GATC 2 cut(s) 346, 640
BssNI GRCGYC 2 cut(s) 80, 303
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 53, 311, 443
BstACI GRCGYC 2 cut(s) 80, 303
BstC8I GCNNGC 2 cut(s) 248, 252
BstDEI CTNAG 3 cut(s) 21, 30, 719
BstENI CCTNNNNNAGG 1 cut(s) 686
BstF5I GGATG 5 cut(s) 199, 295, 350, 424, 765
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 2 cut(s) 349, 643
BstMAI GTCTC 3 cut(s) 67, 386, 624
BstMBI GATC 2 cut(s) 346, 640
BstMWI GCNNNNNNNGC 2 cut(s) 46, 530
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 2 cut(s) 40, 254
BstPAI GACNNNNGTC 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 255, 266
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
Bsu15I ATCGAT 2 cut(s) 209, 744
BsuRI GGCC 2 cut(s) 118, 530
BsuTUI ATCGAT 2 cut(s) 209, 744
BtsCI GGATG 5 cut(s) 199, 295, 350, 424, 765
BtsIMutI CAGTG 2 cut(s) 49, 439
Cac8I GCNNGC 2 cut(s) 248, 252
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 528
ClaI ATCGAT 2 cut(s) 209, 744
CviAII CATG 3 cut(s) 37, 251, 686
DdeI CTNAG 3 cut(s) 21, 30, 719
DinI GGCGCC 1 cut(s) 304
DpnI GATC 2 cut(s) 348, 642
DpnII GATC 2 cut(s) 346, 640
EaeI YGGCCR 1 cut(s) 116
EciI GGCGGA 2 cut(s) 642, 717
EcoNI CCTNNNNNAGG 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 169
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
Esp3I CGTCTC 2 cut(s) 67, 624
FaeI CATG 3 cut(s) 40, 254, 689
FatI CATG 3 cut(s) 36, 250, 685
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 2 cut(s) 264, 274
Fnu4HI GCNGC 3 cut(s) 244, 255, 531
FokI GGATG 4 cut(s) 206, 302, 337, 431
Fsp4HI GCNGC 3 cut(s) 244, 255, 531
FspBI CTAG 2 cut(s) 317, 486
GlaI GCGC 1 cut(s) 304
GluI GCNGC 3 cut(s) 244, 255, 531
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 2 cut(s) 118, 530
HapII CCGG 2 cut(s) 357, 638
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 2 cut(s) 80, 303
Hin1II CATG 3 cut(s) 40, 254, 689
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 1 cut(s) 751
HpaII CCGG 2 cut(s) 357, 638
HphI GGTGA 2 cut(s) 493, 703
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 4 cut(s) 22, 58, 332, 759
Hpy188III TCNNGA 3 cut(s) 160, 283, 317
Hpy8I GTNNAC 1 cut(s) 84
Hpy99I CGWCG 1 cut(s) 759
HpyAV CCTTC 2 cut(s) 280, 293
HpyCH4III ACNGT 3 cut(s) 53, 311, 443
HpyCH4IV ACGT 2 cut(s) 80, 222
HpyCH4V TGCA 3 cut(s) 254, 540, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 530
HpyF3I CTNAG 3 cut(s) 21, 30, 719
HpySE526I ACGT 2 cut(s) 80, 222
Hsp92I GRCGYC 2 cut(s) 80, 303
Hsp92II CATG 3 cut(s) 40, 254, 689
HspAI GCGC 1 cut(s) 303
KasI GGCGCC 1 cut(s) 302
Kzo9I GATC 2 cut(s) 346, 640
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 255, 266
MaeI CTAG 2 cut(s) 317, 486
MaeII ACGT 2 cut(s) 80, 222
MaeIII GTNAC 4 cut(s) 443, 499, 547, 583
MalI GATC 2 cut(s) 348, 642
MbiI CCGCTC 1 cut(s) 608
MboI GATC 2 cut(s) 346, 640
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 118
MluCI AATT 6 cut(s) 91, 183, 293, 365, 553, 655
MluNI TGGCCA 1 cut(s) 118
Mly113I GGCGCC 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 737
Mox20I TGGCCA 1 cut(s) 118
MscI TGGCCA 1 cut(s) 118
MseI TTAA 3 cut(s) 216, 516, 654
Msp20I TGGCCA 1 cut(s) 118
MspA1I CMGCKG 2 cut(s) 233, 506
MspI CCGG 2 cut(s) 357, 638
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 46, 530
NarI GGCGCC 1 cut(s) 303
NdeI CATATG 2 cut(s) 264, 274
NdeII GATC 2 cut(s) 346, 640
NlaIII CATG 3 cut(s) 40, 254, 689
NlaIV GGNNCC 2 cut(s) 304, 495
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 2 cut(s) 40, 254
PaeI GCATGC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 751
PkrI GCNGC 3 cut(s) 245, 256, 532
PluTI GGCGCC 1 cut(s) 306
PshAI GACNNNNGTC 1 cut(s) 79
PsiI TTATAA 2 cut(s) 45, 90
Psp1406I AACGTT 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 304, 495
PspPI GGNCC 1 cut(s) 528
PsuI RGATCY 1 cut(s) 640
SaqAI TTAA 3 cut(s) 216, 516, 654
SatI GCNGC 3 cut(s) 244, 255, 531
Sau3AI GATC 2 cut(s) 346, 640
Sau96I GGNCC 1 cut(s) 528
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 395
SfoI GGCGCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SphI GCATGC 1 cut(s) 254
Sse9I AATT 6 cut(s) 91, 183, 293, 365, 553, 655
SsiI CCGC 6 cut(s) 233, 504, 531, 608, 627, 702
SspDI GGCGCC 1 cut(s) 302
SspMI CTAG 2 cut(s) 317, 486
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 3 cut(s) 53, 311, 443
TaiI ACGT 2 cut(s) 83, 225
TaqI TCGA 2 cut(s) 209, 744
TasI AATT 6 cut(s) 91, 183, 293, 365, 553, 655
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 3 cut(s) 216, 516, 654
Tru9I TTAA 3 cut(s) 216, 516, 654
TscAI CASTG 2 cut(s) 56, 446
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 2 cut(s) 243, 254
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 2 cut(s) 306, 477
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 56, 446
XagI CCTNNNNNAGG 1 cut(s) 686
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 2 cut(s) 40, 254
XspI CTAG 2 cut(s) 317, 486
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.