RchiOBHm_Chr2g0115171

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007612
Physical Location & Seq
Forward (+)
27175370 .. 27178579
3210 bp
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UTR
Exon/CDS
Intron
PRQ48837

Sequence Viewer

Length: 819 bp
ATGGCGCTTGAGTCTTCAATTCTAAGCCCACCCAGATTCATTTCTGGTTCGAAATCATTGAGTTTCTTGTATTACCATAAGCAGAAAAATTTCCCCACCAAGGTTTTGGTGCAAAGTAATCAATACCCATTTCGATTGGTGTCGAATTGCATTGGCACTAGCGATTTTGGGAGGTTCTTAGGTCCAAGATGCGAAGGAGTTGACTTGTTTGCTAATAGAGCTCGTTTGAGAGCTGTAGCTATGGTTTGTGGGCTTTTGGTGTGTGGGTCTAGGAGAGCTTTTGTTGTGGAGGGTGTGGTCAATGCAAGCTATGGGGTTGTTGACAAATGCATATTGATGTTCAGAAATGCTTGGCCGAAGACACTATGGCCTTGGAAGGTGCGTGAGTTGGCTGAGAAAGAGCCTGAAAATGGTGTCTTCAGATTGCTTCGCAATGATGTGACTCGGTTCTTGTCAACCATACTTATTGGCACAACTGTTGTCAATATTAAAGCAACTGCTTTAGTTACAGAGGCTGCAACAGTGATATTTGGTGAAGCTGGTGTTTGTGCAGCAACTGGAGTAATGACTGATATGATTGAAAATGTGTTGGAGATTAAAGATACGCATGTTAGAGAGGTGATGACACCTCTTGTAGATGTAGTTGCAATTGATGCCAGTGCAACGCTAATTGATTTTCACGACTTGTGGGTGACTCATCAATATTCAAGATATGCAAGGACTTTTCTTTTTAATAATTTTAATTTTCATTATTATTATATTTTCCCAAGAAGATTTATACACCTTATTTTGCTTGTTAGGTTAGGCAGGATTAATTGA

Protein Analysis

272

Amino Acids

30.85

Weight (kDa)

9.38

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 122 - 192 5e-09 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 403
AfiI CCNNNNNNNGG 2 cut(s) 100, 410
AgsI TTSAA 3 cut(s) 18, 581, 708
AluBI AGCT 6 cut(s) 221, 233, 239, 278, 309, 539
AluI AGCT 6 cut(s) 221, 233, 239, 278, 309, 539
Alw21I GWGCWC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 515, 557
AoxI GGCC 2 cut(s) 353, 368
ApeKI GCWGC 2 cut(s) 515, 551
ApoI RAATTY 1 cut(s) 88
AseI ATTAAT 1 cut(s) 813
Asp700I GAANNNNTTC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 545, 631, 703
AsuII TTCGAA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 182
BaeI ACNNNNGTAYC 2 cut(s) 594, 627
BanII GRGCYC 1 cut(s) 223
BbsI GAAGAC 3 cut(s) 6, 365, 409
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 502, 563
BfaI CTAG 2 cut(s) 159, 270
BfmI CTRYAG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 516, 552
BlsI GCNGC 2 cut(s) 517, 553
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 182
BmsI GCATC 2 cut(s) 179, 643
BpiI GAAGAC 3 cut(s) 6, 365, 409
BpmI CTGGAG 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 99, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 410
Bse1I ACTGG 2 cut(s) 562, 657
Bse3DI GCAATG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 99, 371
BseLI CCNNNNNNNGG 2 cut(s) 100, 410
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 562, 657
BseXI GCAGC 2 cut(s) 502, 563
BsgI GTGCAG 1 cut(s) 570
BshFI GGCC 2 cut(s) 355, 370
BsiHKAI GWGCWC 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 100, 410
BsnI GGCC 2 cut(s) 355, 370
Bsp119I TTCGAA 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 223
BspANI GGCC 2 cut(s) 355, 370
BspCNI CTCAG 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 439
BsrI ACTGG 2 cut(s) 562, 657
BssECI CCNNGG 2 cut(s) 99, 371
BssT1I CCWWGG 2 cut(s) 99, 371
Bst4CI ACNGT 2 cut(s) 478, 523
BstAPI GCANNNNNTGC 1 cut(s) 653
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 307
BstDEI CTNAG 3 cut(s) 23, 178, 393
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMWI GCNNNNNNNGC 2 cut(s) 218, 653
BstNSI RCATGY 1 cut(s) 611
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 502, 563
BstV2I GAAGAC 3 cut(s) 6, 365, 409
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 355, 370
BtsIMutI CAGTG 2 cut(s) 528, 664
Cac8I GCNNGC 1 cut(s) 307
CaiI CAGNNNCTG 2 cut(s) 515, 557
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 182
CviAII CATG 1 cut(s) 608
DdeI CTNAG 3 cut(s) 23, 178, 393
EaeI YGGCCR 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 2 cut(s) 99, 371
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 182
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 221
EcoT14I CCWWGG 2 cut(s) 99, 371
EcoT22I ATGCAT 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 99, 371
FaeI CATG 1 cut(s) 611
FatI CATG 1 cut(s) 607
Fnu4HI GCNGC 2 cut(s) 516, 552
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 2 cut(s) 516, 552
FspBI CTAG 2 cut(s) 159, 270
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 516, 552
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 355, 370
HhaI GCGC 1 cut(s) 7
Hin1II CATG 1 cut(s) 611
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 202, 322, 456
HindII GTYRAC 3 cut(s) 202, 322, 456
HinfI GANTC 4 cut(s) 11, 36, 442, 694
HphI GGTGA 3 cut(s) 545, 631, 703
Hpy166II GTNNAC 3 cut(s) 202, 322, 456
Hpy188I TCNGA 2 cut(s) 344, 422
Hpy188III TCNNGA 2 cut(s) 680, 708
Hpy8I GTNNAC 3 cut(s) 202, 322, 456
HpyAV CCTTC 2 cut(s) 188, 370
HpyCH4III ACNGT 2 cut(s) 478, 523
HpyCH4V TGCA 9 cut(s) 112, 150, 305, 330, 518, 551, 647, 662, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 653
HpyF3I CTNAG 3 cut(s) 23, 178, 393
Hsp92II CATG 1 cut(s) 611
HspAI GCGC 1 cut(s) 5
LpnPI CCDG 7 cut(s) 30, 46, 417, 525, 543, 670, 793
Lsp1109I GCAGC 2 cut(s) 502, 563
LweI GCATC 2 cut(s) 179, 643
MaeI CTAG 2 cut(s) 159, 270
MaeIII GTNAC 3 cut(s) 439, 505, 691
MboII GAAGA 4 cut(s) 6, 370, 409, 783
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 8 cut(s) 18, 88, 145, 648, 669, 736, 742, 814
MlyI GAGTC 3 cut(s) 20, 436, 688
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 5 cut(s) 165, 283, 505, 610, 639
Mph1103I ATGCAT 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 5 cut(s) 489, 597, 732, 741, 813
MslI CAYNNNNRTG 1 cut(s) 335
MunI CAATTG 1 cut(s) 648
MwoI GCNNNNNNNGC 2 cut(s) 218, 653
NlaIII CATG 1 cut(s) 611
NmuCI GTSAC 2 cut(s) 439, 691
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 1 cut(s) 611
NspV TTCGAA 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 2 cut(s) 517, 553
PleI GAGTC 3 cut(s) 19, 436, 688
PpsI GAGTC 3 cut(s) 19, 436, 688
PshBI ATTAAT 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 182
PstNI CAGNNNCTG 2 cut(s) 515, 557
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 5 cut(s) 489, 597, 732, 741, 813
SatI GCNGC 2 cut(s) 516, 552
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 3 cut(s) 20, 436, 688
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 2 cut(s) 179, 643
SfcI CTRYAG 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 50
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 8 cut(s) 18, 88, 145, 648, 669, 736, 742, 814
SspI AATATT 2 cut(s) 487, 704
SspMI CTAG 2 cut(s) 159, 270
SstI GAGCTC 1 cut(s) 223
StyI CCWWGG 2 cut(s) 99, 371
TaaI ACNGT 2 cut(s) 478, 523
TaqI TCGA 3 cut(s) 50, 133, 143
TasI AATT 8 cut(s) 18, 88, 145, 648, 669, 736, 742, 814
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 5 cut(s) 489, 597, 732, 741, 813
Tru9I TTAA 5 cut(s) 489, 597, 732, 741, 813
TscAI CASTG 2 cut(s) 528, 664
TseFI GTSAC 2 cut(s) 439, 691
TseI GCWGC 2 cut(s) 515, 551
Tsp45I GTSAC 2 cut(s) 439, 691
TspDTI ATGAA 2 cut(s) 28, 737
TspRI CASTG 2 cut(s) 528, 664
VpaK11BI GGWCC 1 cut(s) 182
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 2 cut(s) 159, 270
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.