RchiOBHm_Chr5g0043101

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007612
Physical Location & Seq
Reverse (-)
37952230 .. 37955740
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32143

Sequence Viewer

Length: 1185 bp
ATGGCGCTTGAGTCTTCAATTCTAAGCCCGCCCAGATTCATTTCTGGTTCTGGTTCGAAATCATTGTGTTTCTTGTATTACCATAAGCAGAAAAATTTCCCCACCAAGGTTTTGGTGCAAAATAATCGATACCCATTTCGATTGGTGTTGAATTGGATTGACACTAGCGATTTTGGGAGGTTCTTAGGTCCAAGATGCGAGGGAGTCGACTTGTTTGCGAATAGAGCTTGTTTGAGAGCTGTAGGTAAAGATAGTGAGAGTTCTAGTGGTACAAATGCTATTTTGGGTTTGAGTTTTGATTTTGTGAGGGTAATGGTGAAGTGTGGAGTTGTTTTAGCGGCTATGGTTTGTGGGGTTTTGGTGTATGGGGCTAGGAGAGCTTTTGCTGTGGAGGGTGTGGTCAATGCAAGCTATGGGGTTGTTGACAAGTGCATGTTGATGTTCAGAAATGCTTGGCCGAAGACGCTATGGCCTTGGAAGGTGTGTGAGTTGGCTGAGAAAGAGCCTGAAAATGGTGTCTTCAGTTTGCTTCGCAATGATGTGACTCGGTTCTTGTCAACCATACTTATTGGCACAACTGTTGTCAATATTAGAGCAACTGCTTTAGTTACAGAGGCTGCAACAGCGATATTTGGTGAAGCTGGTGTTTGTGCAGCAACTGGAGTAATGACTGTTCTTATTTTGCTCCTCACTGAAATAACTCCCAAAAGTGTTTCTGTTCACAATCCCACAGAAGTTGCTAGGTTTGTGGTTAGGCCGGTGGCTTGGCTTTCATTGGTACTATATCCTGTCGGAAGAGTTGGTACATACCTATCAATGGGGATGCTGAAAATTCTAGGGTTGAAAGGAAGAAGTGAACCATATGTTACTGAAGAGGAGTTAAAACTGATGTTGCGAGCGGCAGAGTTAAGTGGGGCAATAGAGGAGGAAGAACAGGATATGATTGAAAATGTGTTGGAGATTAAAGATACGCATGTTAGAGAGGTGATGACACCTCTTGTAGATGTAGTTGCAATTGATGCCAGTGCAACGCTAATTGATTTTCACGACTTGTGGGTGACTCATCAATATTCAAGATATGCAAGGACTTTTCTTTTTAATAATTTTAATTTTCATTATTATTATATTTTCCCAAGAAGATTTATACACCTTATTTTGCTTGTTAGGTTAGGCAGGATTAATTGA

Protein Analysis

394

Amino Acids

44.11

Weight (kDa)

8.55

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 159 - 311 5.2e-37 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 899
AccI GTMKAC 1 cut(s) 207
AciI CCGC 3 cut(s) 29, 338, 899
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 2 cut(s) 94, 831
AcuI CTGAAG 2 cut(s) 505, 891
AfaI GTAC 3 cut(s) 271, 780, 805
AfiI CCNNNNNNNGG 3 cut(s) 106, 512, 817
AgsI TTSAA 5 cut(s) 18, 151, 844, 947, 1074
AluBI AGCT 5 cut(s) 227, 239, 380, 411, 641
AluI AGCT 5 cut(s) 227, 239, 380, 411, 641
AlwNI CAGNNNCTG 2 cut(s) 617, 659
AoxI GGCC 3 cut(s) 455, 470, 755
ApeKI GCWGC 2 cut(s) 617, 653
ApoI RAATTY 2 cut(s) 94, 831
AseI ATTAAT 1 cut(s) 1179
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 4 cut(s) 328, 647, 997, 1069
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 1 cut(s) 188
BaeI ACNNNNGTAYC 2 cut(s) 960, 993
BarI GAAGNNNNNNTAC 2 cut(s) 787, 819
BbsI GAAGAC 3 cut(s) 6, 467, 511
BbvI GCAGC 2 cut(s) 604, 665
BcgI CGANNNNNNTGC 4 cut(s) 107, 141, 197, 231
BfaI CTAG 5 cut(s) 165, 264, 372, 741, 836
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 4 cut(s) 339, 618, 654, 900
BlsI GCNGC 4 cut(s) 340, 619, 655, 901
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmsI GCATC 3 cut(s) 185, 813, 1009
BpiI GAAGAC 3 cut(s) 6, 467, 511
BpmI CTGGAG 1 cut(s) 681
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 105, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 512, 817
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 2 cut(s) 664, 1023
Bse3DI GCAATG 1 cut(s) 541
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 105, 473
BseGI GGATG 1 cut(s) 828
BseLI CCNNNNNNNGG 3 cut(s) 106, 512, 817
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 664, 1023
BseRI GAGGAG 3 cut(s) 677, 890, 938
BseXI GCAGC 2 cut(s) 604, 665
BsgI GTGCAG 1 cut(s) 672
BshFI GGCC 3 cut(s) 457, 472, 757
BshVI ATCGAT 1 cut(s) 127
BsiSI CCGG 1 cut(s) 758
BslI CCNNNNNNNGG 3 cut(s) 106, 512, 817
BsnI GGCC 3 cut(s) 457, 472, 757
Bsp119I TTCGAA 1 cut(s) 56
BspACI CCGC 3 cut(s) 29, 338, 899
BspANI GGCC 3 cut(s) 457, 472, 757
BspCNI CTCAG 1 cut(s) 487
BspDI ATCGAT 1 cut(s) 127
BspT104I TTCGAA 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 899
BsrDI GCAATG 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 2 cut(s) 664, 1023
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 2 cut(s) 105, 473
BssT1I CCWWGG 2 cut(s) 105, 473
Bst4CI ACNGT 2 cut(s) 580, 673
Bst6I CTCTTC 2 cut(s) 790, 867
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 29, 409, 897
BstDEI CTNAG 3 cut(s) 23, 184, 495
BstF5I GGATG 1 cut(s) 828
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMWI GCNNNNNNNGC 5 cut(s) 224, 377, 463, 623, 1019
BstNSI RCATGY 2 cut(s) 436, 977
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 604, 665
BstV2I GAAGAC 3 cut(s) 6, 467, 511
BstXI CCANNNNNNTGG 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 127
BsuRI GGCC 3 cut(s) 457, 472, 757
BsuTUI ATCGAT 1 cut(s) 127
BtsCI GGATG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 690, 1030
Cac8I GCNNGC 3 cut(s) 29, 409, 897
CaiI CAGNNNCTG 2 cut(s) 617, 659
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 188
ClaI ATCGAT 1 cut(s) 127
CseI GACGC 1 cut(s) 472
Csp6I GTAC 3 cut(s) 270, 779, 804
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 2 cut(s) 433, 974
CviQI GTAC 3 cut(s) 270, 779, 804
DdeI CTNAG 3 cut(s) 23, 184, 495
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 2 cut(s) 790, 867
EarI CTCTTC 2 cut(s) 790, 867
Eco130I CCWWGG 2 cut(s) 105, 473
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 505, 891
EcoT14I CCWWGG 2 cut(s) 105, 473
ErhI CCWWGG 2 cut(s) 105, 473
FaeI CATG 2 cut(s) 436, 977
FatI CATG 2 cut(s) 432, 973
FauI CCCGC 1 cut(s) 36
FauNDI CATATG 1 cut(s) 862
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 339, 618, 654, 900
FokI GGATG 1 cut(s) 835
Fsp4HI GCNGC 4 cut(s) 339, 618, 654, 900
FspBI CTAG 5 cut(s) 165, 264, 372, 741, 836
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 339, 618, 654, 900
GsuI CTGGAG 1 cut(s) 681
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 457, 472, 757
HapII CCGG 1 cut(s) 758
HgaI GACGC 1 cut(s) 472
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 436, 977
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 208, 424, 558
HindII GTYRAC 3 cut(s) 208, 424, 558
HinfI GANTC 5 cut(s) 11, 36, 204, 544, 1060
HpaII CCGG 1 cut(s) 758
HphI GGTGA 4 cut(s) 328, 647, 997, 1069
Hpy166II GTNNAC 5 cut(s) 208, 424, 558, 721, 857
Hpy188I TCNGA 2 cut(s) 446, 794
Hpy188III TCNNGA 2 cut(s) 1046, 1074
Hpy8I GTNNAC 5 cut(s) 208, 424, 558, 721, 857
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 2 cut(s) 580, 673
HpyCH4V TGCA 8 cut(s) 118, 407, 432, 620, 653, 1013, 1028, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 377, 463, 623, 1019
HpyF3I CTNAG 3 cut(s) 23, 184, 495
Hsp92II CATG 2 cut(s) 436, 977
HspAI GCGC 1 cut(s) 5
LmnI GCTCC 1 cut(s) 690
Lsp1109I GCAGC 2 cut(s) 604, 665
LweI GCATC 3 cut(s) 185, 813, 1009
MaeI CTAG 5 cut(s) 165, 264, 372, 741, 836
MaeIII GTNAC 4 cut(s) 541, 607, 865, 1057
MbiI CCGCTC 1 cut(s) 899
MboII GAAGA 8 cut(s) 6, 472, 511, 807, 861, 884, 941, 1149
MfeI CAATTG 1 cut(s) 1014
MluCI AATT 9 cut(s) 18, 94, 151, 831, 1014, 1035, 1102, 1108, 1180
MlyI GAGTC 4 cut(s) 20, 213, 538, 1054
MmeI TCCRAC 2 cut(s) 772, 936
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 6 cut(s) 881, 908, 963, 1098, 1107, 1179
MslI CAYNNNNRTG 1 cut(s) 437
MspI CCGG 1 cut(s) 758
MunI CAATTG 1 cut(s) 1014
MwoI GCNNNNNNNGC 5 cut(s) 224, 377, 463, 623, 1019
NdeI CATATG 1 cut(s) 862
NlaIII CATG 2 cut(s) 436, 977
NmuCI GTSAC 2 cut(s) 541, 1057
NspI RCATGY 2 cut(s) 436, 977
NspV TTCGAA 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 4 cut(s) 340, 619, 655, 901
PleI GAGTC 4 cut(s) 19, 212, 538, 1054
PpsI GAGTC 4 cut(s) 19, 212, 538, 1054
PshBI ATTAAT 1 cut(s) 1179
PspPI GGNCC 1 cut(s) 188
PsrI GAACNNNNNNTAC 2 cut(s) 657, 689
PstNI CAGNNNCTG 2 cut(s) 617, 659
RsaI GTAC 3 cut(s) 271, 780, 805
RsaNI GTAC 3 cut(s) 270, 779, 804
RseI CAYNNNNRTG 1 cut(s) 437
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 6 cut(s) 881, 908, 963, 1098, 1107, 1179
SatI GCNGC 4 cut(s) 339, 618, 654, 900
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 4 cut(s) 20, 213, 538, 1054
SfaNI GCATC 3 cut(s) 185, 813, 1009
SfcI CTRYAG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 9 cut(s) 18, 94, 151, 831, 1014, 1035, 1102, 1108, 1180
SsiI CCGC 3 cut(s) 29, 338, 899
SspI AATATT 2 cut(s) 589, 1070
SspMI CTAG 5 cut(s) 165, 264, 372, 741, 836
StyI CCWWGG 2 cut(s) 105, 473
TaaI ACNGT 2 cut(s) 580, 673
TaqI TCGA 4 cut(s) 56, 127, 139, 207
TasI AATT 9 cut(s) 18, 94, 151, 831, 1014, 1035, 1102, 1108, 1180
TauI GCSGC 2 cut(s) 341, 902
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 6 cut(s) 881, 908, 963, 1098, 1107, 1179
Tru9I TTAA 6 cut(s) 881, 908, 963, 1098, 1107, 1179
TscAI CASTG 2 cut(s) 697, 1030
TseFI GTSAC 2 cut(s) 541, 1057
TseI GCWGC 2 cut(s) 617, 653
Tsp45I GTSAC 2 cut(s) 541, 1057
TspDTI ATGAA 3 cut(s) 28, 762, 1103
TspRI CASTG 2 cut(s) 697, 1030
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 2 cut(s) 94, 831
XceI RCATGY 2 cut(s) 436, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 5 cut(s) 165, 264, 372, 741, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.