Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003124

Physical Location & Seq

Genomic Coordinates Reverse (-)

28863578 .. 28869717

Gene Structure 6140 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ48958

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGCCGCTGTGTGCACTCCCCACACAATCTCACCCGCCGCCATAGTTTCTTCTTCTCCAGTCCGATGCTCTCACTCCCAATCGCAGCTCCTATTCCGTTCAAATCTCCCGAACCGCCGTCACCACAGCCCGAGCCTCCCGATTTCGGCTCCCAATCTCCGCGCCGCTACCAGAGCACGAGTTCTCTGCTCACAAAACCCTAACCCTTCCCCCACGCGCCGCCCCAACTACGTCCCCAACCGCATCTCCGACCCGAACTATGTCCGCATATTCGACACCACGCTCCGCGACGGCGAGCAGTCCCCCGGCGCCTCGCTCACCTCCAAGGAGAAGCTCGACATTGCGCGCCAGCTGGCCAAGCTCGGCGTCGACATAATCGAGGCCGGGTTCCCTGCGGCGTCGAAGGACGACTTCGAAGCCGTGAAGATGATCGCGAAAGAGGTCGGGAATTTCGTGGATAAAGACGGTTATGTCCCTGTGATCTGTGGGCTGTCCAGGTGCAATGAGAAGGATATTAAGACGGCGTGGGAGGCGGTGAAGTACGCAAAGAGGCCGAGGGTTCATACTTTCATTGCTACCAGTCCGATTCATATGGAGCATAAGCTGAGGAAGACGAAGGAGCAGGTGATCGAGATTGCGAGGAGCATGGTGAGCTACGCTAGGAGCTTGGGTTGCCAAGATGTCGAGTTTAGCCCTGAAGATGCTGGCAGATCTGAGAAGGAATTTCTATATCAGATTTTAGGTGAAGTTATAAAGGCTGGGGCAACAACTCTGAACATTCCTGACACTGTGGGTTATACCGTGCCAACTGAATTTGGTCAGTTGATTGCTGACATTAAATCTAATACCCCTGGAATTGATAACGTGATCATCTCGACTCACTGTCAAAATGATCTTGGACTTTCTACCGCCAACACTTTAGCGGGGGCAAGTAGAGGTGCTAGGCAATTGGAAGTAACAATCAATGGAATTGGTGAAAGGGCTGGTAATGCTTCATTAGAGGAGGTTGTCATGACCTTAAACTGCCGTGGGGAGCATGTACTTGGGGGGCTTTATACTGGAATCAATCCTCGGCATATTTATATGACGAGCAAGATGGTTTCAGAGTACACTGGGATGCATGTGCAGCCACACAAGGCTATTGTTGGATCTAATGCTTTTGCACATGAAAGTGGTATCCATCAGGATGGAATGCTCAAGGACAAACGTACATATGAAATTATATGTCCTGAAGATATTGGGTATGACCGGTCCAATGAAGCTGGCATTGTCCTAGGGAAACTTAGTGGACGCCATGCTGTGAAAAAGCAACTTGAACAGCTTGGTTATGAGCTTGATGATGTTCAGCTTGAGACTGTATTCTGGCGTTTCAAAGGAGTAGCTGATCAGAAAAAGAGAGTCACTGATGCAGACCTCAGAGCATTGGTGTCAGATGAAGTTTTTCAGCCAGAAGTTGTCTGGAAACTTCATGATCTACAGGTTACCTGTGGAACTCTTGGTCTTTCTACAGCAACTGTTAAACTAATTGATGCTGATGGGAGAGAGCATATTGCATGTTCAGTTGGAACGGGTCCAGTGGATTCAGCTTACAAGGCTGTTGATCTCATTGTGAAGGAACCAGTAACACTTGTTGAGTACTCTATGAATGCGGTCACAGAGGGAATAGATGCAATAGCCACTACTCGTGTTGCAATTCGAGTTGAAAATAGTGATACGGTCACTGGTGAGATATCTCAACGGACATTCAGTGGGATTGGGGCAGGAATGGACATTGTTGTTTCTAGTGTCAAGGCCTACATTGGTGCATTGAACAAGATGCTAGGTTTCAAGGAAAGGTTTCCATTAGAGGTTGCAGCAGAATCAGCAGAACGAACTGCAGTGTCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003852 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006551 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009098 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0019752 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046912 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00620 ko01100 ko01110 ko01210 ko01230 map00290 map00620 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

HMGL-like LeuA_dimer

Protein Analysis

629

Amino Acids

68.45

Weight (kDa)

6.52

Isoelectric Point (pI)

40.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMGL-like	PF00682	88 - 367	3.4e-89	HMGL-like
HCS_D2	PF22617	381 - 465	1.8e-28	Homocitrate synthase post-HMGL domain-like
LeuA_dimer	PF08502	466 - 607	3.5e-32	LeuA allosteric (dimerisation) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18500 AT1G74040 AT1G74040 AT1G74040
fragaria_vesca	FvH4_1g22410 FvH4_1g22460 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570
malus_domestica	MD01G1019700.v1.1 MD15G1332600.v1.1
prunus_persica	Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1
pyrus_communis	pycom01g04920 pycom15g29280
rosa_chinensis	RchiOBHm_Chr2g0116551 RchiOBHm_Chr2g0116561
rosa_laevigata	RLG00000018287 RLG00000018288
rosa_multiflora	Rmu_sc0001287.1_g000019 Rmu_sc0004191.1_g000022 Rmu_sc0004191.1_g000030
rosa_roxburghii	Rroxscaffold_2G00127060 Rroxscaffold_2G00127070
rosa_rugosa	Rorug02G0207600
rosa_samantha	Rh2AG262500 Rh2AG262600 Rh2BG274100 Rh2BG274400 Rh2DG270700 Rh2DG270800 Rh2DG289200 Rh2DG289300
rosa_wichuraiana	Rw2G020670 Rw2G020680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	752
AarI	CACCTGC	1 cut(s)	613
AasI	GACNNNNNNGTC	1 cut(s)	470
Acc36I	ACCTGC	1 cut(s)	613
AccB1I	GGYRCC	1 cut(s)	308
AccI	GTMKAC	1 cut(s)	369
AccII	CGCG	5 cut(s)	162, 217, 288, 346, 434
AclWI	GGATC	1 cut(s)	1156
AcoI	YGGCCR	2 cut(s)	3, 354
AcsI	RAATTY	3 cut(s)	448, 722, 812
AcuI	CTGAAG	2 cut(s)	717, 1251
AcyI	GRCGYC	4 cut(s)	309, 366, 398, 1291
AfaI	GTAC	5 cut(s)	542, 1041, 1109, 1210, 1637
AfiI	CCNNNNNNNGG	1 cut(s)	145
AgeI	ACCGGT	1 cut(s)	1248
AgsI	TTSAA	6 cut(s)	102, 1316, 1372, 1703, 1810, 1828
AhdI	GACNNNNNGTC	1 cut(s)	882
AjnI	CCWGG	2 cut(s)	494, 850
Alw21I	GWGCWC	2 cut(s)	17, 178
Alw26I	GTCTC	1 cut(s)	1346
Alw44I	GTGCAC	1 cut(s)	13
AlwI	GGATC	1 cut(s)	1156
AlwNI	CAGNNNCTG	2 cut(s)	1514, 1883
Ama87I	CYCGRG	1 cut(s)	130
AoxI	GGCC	5 cut(s)	3, 354, 381, 551, 1791
ApaLI	GTGCAC	1 cut(s)	13
ApeKI	GCWGC	3 cut(s)	85, 1126, 1853
ApoI	RAATTY	3 cut(s)	448, 722, 812
AsiGI	ACCGGT	1 cut(s)	1248
Asp700I	GAANNNNTTC	2 cut(s)	1440, 1836
AspA2I	CCTAGG	1 cut(s)	1273
AspLEI	GCGC	5 cut(s)	164, 219, 311, 346, 348
AspS9I	GGNCC	2 cut(s)	1251, 1571
AsuC2I	CCSGG	2 cut(s)	306, 385
AsuHPI	GGTGA	9 cut(s)	24, 113, 310, 547, 637, 661, 755, 986, 1736
AsuII	TTCGAA	1 cut(s)	414
AvaI	CYCGRG	1 cut(s)	130
AvaII	GGWCC	2 cut(s)	1251, 1571
AvrII	CCTAGG	1 cut(s)	1273
BaeGI	GKGCMC	1 cut(s)	17
BalI	TGGCCA	1 cut(s)	356
BanI	GGYRCC	1 cut(s)	308
BauI	CACGAG	2 cut(s)	177, 1683
BbsI	GAAGAC	1 cut(s)	617
Bbv12I	GWGCWC	2 cut(s)	17, 178
BbvCI	CCTCAGC	1 cut(s)	605
BbvI	GCAGC	3 cut(s)	97, 1138, 1865
BccI	CCATC	4 cut(s)	1090, 1181, 1188, 1529
BceAI	ACGGC	5 cut(s)	102, 307, 404, 537, 1011
BcgI	CGANNNNNNTGC	2 cut(s)	168, 202
BciT130I	CCWGG	2 cut(s)	496, 852
BciVI	GTATCC	1 cut(s)	1187
BclI	TGATCA	2 cut(s)	867, 1384
BcnI	CCSGG	2 cut(s)	306, 385
BcoDI	GTCTC	1 cut(s)	1346
BfaI	CTAG	5 cut(s)	660, 942, 1274, 1782, 1820
BfmI	CTRYAG	3 cut(s)	1475, 1506, 1875
BfoI	RGCGCY	1 cut(s)	312
BfuAI	ACCTGC	1 cut(s)	613
BfuI	GTATCC	1 cut(s)	1187
BglII	AGATCT	1 cut(s)	710
BisI	GCNGC	8 cut(s)	6, 39, 86, 165, 220, 396, 1127, 1854
BlnI	CCTAGG	1 cut(s)	1273
BlsI	GCNGC	8 cut(s)	7, 40, 87, 166, 221, 397, 1128, 1855
BmcAI	AGTACT	1 cut(s)	1637
Bme1390I	CCNGG	4 cut(s)	306, 385, 496, 852
Bme18I	GGWCC	2 cut(s)	1251, 1571
BmeRI	GACNNNNNGTC	1 cut(s)	882
BmeT110I	CYCGRG	1 cut(s)	130
BmgT120I	GGNCC	2 cut(s)	1251, 1571
BmiI	GGNNCC	5 cut(s)	150, 310, 389, 1572, 1617
BmrFI	CCNGG	4 cut(s)	306, 385, 496, 852
BmrI	ACTGGG	1 cut(s)	1122
BmsI	GCATC	8 cut(s)	56, 252, 691, 1107, 1396, 1519, 1657, 1806
BmuI	ACTGGG	1 cut(s)	1122
BpiI	GAAGAC	1 cut(s)	617
BpmI	CTGGAG	1 cut(s)	42
Bpu10I	CCTNAGC	1 cut(s)	605
Bpu14I	TTCGAA	1 cut(s)	414
BpuEI	CTTGAG	2 cut(s)	1181, 1370
BpuMI	CCSGG	2 cut(s)	306, 385
BsaHI	GRCGYC	4 cut(s)	309, 366, 398, 1291
BsaJI	CCNNGG	7 cut(s)	304, 324, 554, 850, 1027, 1070, 1273
BsaWI	WCCGGW	1 cut(s)	1248
BsaXI	ACNNNNNCTCC	4 cut(s)	230, 260, 655, 685
Bsc4I	CCNNNNNNNGG	1 cut(s)	145
Bse118I	RCCGGY	1 cut(s)	1248
Bse1I	ACTGG	7 cut(s)	59, 579, 1063, 1117, 1574, 1619, 1726
Bse3DI	GCAATG	3 cut(s)	339, 508, 570
BseBI	CCWGG	2 cut(s)	496, 852
BseDI	CCNNGG	7 cut(s)	304, 324, 554, 850, 1027, 1070, 1273
BseGI	GGATG	2 cut(s)	1122, 1192
BseLI	CCNNNNNNNGG	1 cut(s)	145
BseMI	GCAATG	3 cut(s)	339, 508, 570
BseMII	CTCAG	3 cut(s)	596, 705, 1429
BseNI	ACTGG	7 cut(s)	59, 579, 1063, 1117, 1574, 1619, 1726
BsePI	GCGCGC	1 cut(s)	344
BseRI	GAGGAG	2 cut(s)	655, 1016
BseSI	GKGCMC	1 cut(s)	17
BseXI	GCAGC	3 cut(s)	97, 1138, 1865
BseYI	CCCAGC	1 cut(s)	758
BsgI	GTGCAG	1 cut(s)	1145
Bsh1236I	CGCG	5 cut(s)	162, 217, 288, 346, 434
BshFI	GGCC	5 cut(s)	5, 356, 383, 553, 1793
BshNI	GGYRCC	1 cut(s)	308
BshTI	ACCGGT	1 cut(s)	1248
BsiHKAI	GWGCWC	2 cut(s)	17, 178
BsiHKCI	CYCGRG	1 cut(s)	130
BsiSI	CCGG	3 cut(s)	306, 384, 1249
BslFI	GGGAC	3 cut(s)	218, 286, 458
BslI	CCNNNNNNNGG	1 cut(s)	145
BsmAI	GTCTC	1 cut(s)	1346
BsmFI	GGGAC	3 cut(s)	218, 286, 458
BsmI	GAATGC	2 cut(s)	1197, 1651
BsnI	GGCC	5 cut(s)	5, 356, 383, 553, 1793
BsoBI	CYCGRG	1 cut(s)	130
Bsp119I	TTCGAA	1 cut(s)	414
Bsp1286I	GDGCHC	2 cut(s)	17, 178
Bsp68I	TCGCGA	1 cut(s)	434
BspANI	GGCC	5 cut(s)	5, 356, 383, 553, 1793
BspCNI	CTCAG	3 cut(s)	597, 706, 1428
BspFNI	CGCG	5 cut(s)	162, 217, 288, 346, 434
BspHI	TCATGA	2 cut(s)	1011, 1468
BspLI	GGNNCC	5 cut(s)	150, 310, 389, 1572, 1617
BspMAI	CTGCAG	1 cut(s)	1879
BspMI	ACCTGC	1 cut(s)	613
BspPI	GGATC	1 cut(s)	1156
BspT104I	TTCGAA	1 cut(s)	414
BspT107I	GGYRCC	1 cut(s)	308
BsrDI	GCAATG	3 cut(s)	339, 508, 570
BsrFI	RCCGGY	1 cut(s)	1248
BsrI	ACTGG	7 cut(s)	59, 579, 1063, 1117, 1574, 1619, 1726
BssAI	RCCGGY	1 cut(s)	1248
BssECI	CCNNGG	7 cut(s)	304, 324, 554, 850, 1027, 1070, 1273
BssHII	GCGCGC	1 cut(s)	344
BssNI	GRCGYC	4 cut(s)	309, 366, 398, 1291
BssSI	CACGAG	2 cut(s)	177, 1683
BssT1I	CCWWGG	2 cut(s)	324, 1273
Bst2BI	CACGAG	2 cut(s)	177, 1683
Bst2UI	CCWGG	2 cut(s)	496, 852
Bst4CI	ACNGT	7 cut(s)	467, 790, 802, 884, 1357, 1516, 1717
BstACI	GRCGYC	4 cut(s)	309, 366, 398, 1291
BstAPI	GCANNNNNTGC	1 cut(s)	1883
BstBI	TTCGAA	1 cut(s)	414
BstC8I	GCNNGC	6 cut(s)	296, 346, 350, 354, 706, 1264
BstDEI	CTNAG	4 cut(s)	605, 714, 1283, 1415
BstDSI	CCRYGG	1 cut(s)	1027
BstEII	GGTNACC	1 cut(s)	1480
BstF5I	GGATG	2 cut(s)	1122, 1192
BstFNI	CGCG	5 cut(s)	162, 217, 288, 346, 434
BstH2I	RGCGCY	1 cut(s)	312
BstHHI	GCGC	5 cut(s)	164, 219, 311, 346, 348
BstMAI	GTCTC	1 cut(s)	1346
BstNI	CCWGG	2 cut(s)	496, 852
BstNSI	RCATGY	3 cut(s)	1040, 1124, 1557
BstPI	GGTNACC	1 cut(s)	1480
BstSCI	CCNGG	4 cut(s)	304, 383, 494, 850
BstSFI	CTRYAG	3 cut(s)	1475, 1506, 1875
BstSLI	GKGCMC	1 cut(s)	17
BstUI	CGCG	5 cut(s)	162, 217, 288, 346, 434
BstV1I	GCAGC	3 cut(s)	97, 1138, 1865
BstV2I	GAAGAC	1 cut(s)	617
BstX2I	RGATCY	2 cut(s)	710, 1148
BstXI	CCANNNNNNTGG	1 cut(s)	1187
BstYI	RGATCY	2 cut(s)	710, 1148
BsuI	GTATCC	1 cut(s)	1187
BsuRI	GGCC	5 cut(s)	5, 356, 383, 553, 1793
BtgI	CCRYGG	1 cut(s)	1027
BtsCI	GGATG	2 cut(s)	1122, 1192
BtsI	GCAGTG	1 cut(s)	1884
BtsIMutI	CAGTG	8 cut(s)	786, 880, 1110, 1401, 1581, 1719, 1753, 1884
BtuMI	TCGCGA	1 cut(s)	434
BveI	ACCTGC	1 cut(s)	613
Cac8I	GCNNGC	6 cut(s)	296, 346, 350, 354, 706, 1264
CaiI	CAGNNNCTG	2 cut(s)	1514, 1883
CciI	TCATGA	2 cut(s)	1011, 1468
CfoI	GCGC	5 cut(s)	164, 219, 311, 346, 348
Cfr10I	RCCGGY	1 cut(s)	1248
Cfr13I	GGNCC	2 cut(s)	1251, 1571
CseI	GACGC	3 cut(s)	355, 387, 1299
Csp6I	GTAC	5 cut(s)	541, 1040, 1108, 1209, 1636
CspAI	ACCGGT	1 cut(s)	1248
CviAII	CATG	8 cut(s)	646, 1012, 1037, 1121, 1166, 1295, 1469, 1554
CviQI	GTAC	5 cut(s)	541, 1040, 1108, 1209, 1636
DdeI	CTNAG	4 cut(s)	605, 714, 1283, 1415
DinI	GGCGCC	1 cut(s)	310
DrdI	GACNNNNNNGTC	1 cut(s)	470
DriI	GACNNNNNGTC	1 cut(s)	882
DseDI	GACNNNNNNGTC	1 cut(s)	470
EaeI	YGGCCR	2 cut(s)	3, 354
Eam1105I	GACNNNNNGTC	1 cut(s)	882
Eco130I	CCWWGG	2 cut(s)	324, 1273
Eco147I	AGGCCT	1 cut(s)	1793
Eco32I	GATATC	1 cut(s)	1731
Eco47I	GGWCC	2 cut(s)	1251, 1571
Eco57I	CTGAAG	2 cut(s)	717, 1251
Eco88I	CYCGRG	1 cut(s)	130
Eco91I	GGTNACC	1 cut(s)	1480
EcoO65I	GGTNACC	1 cut(s)	1480
EcoRII	CCWGG	2 cut(s)	494, 850
EcoRV	GATATC	1 cut(s)	1731
EcoT14I	CCWWGG	2 cut(s)	324, 1273
EcoT22I	ATGCAT	1 cut(s)	1122
EgeI	GGCGCC	1 cut(s)	310
EheI	GGCGCC	1 cut(s)	310
ErhI	CCWWGG	2 cut(s)	324, 1273
FaeI	CATG	8 cut(s)	649, 1015, 1040, 1124, 1169, 1298, 1472, 1557
FalI	AAGNNNNNCTT	2 cut(s)	395, 427
FaqI	GGGAC	3 cut(s)	218, 286, 458
FatI	CATG	8 cut(s)	645, 1011, 1036, 1120, 1165, 1294, 1468, 1553
FauI	CCCGC	2 cut(s)	43, 916
FauNDI	CATATG	2 cut(s)	591, 1213
FbaI	TGATCA	2 cut(s)	867, 1384
FblI	GTMKAC	1 cut(s)	369
Fnu4HI	GCNGC	8 cut(s)	6, 39, 86, 165, 220, 396, 1127, 1854
FokI	GGATG	2 cut(s)	1129, 1199
Fsp4HI	GCNGC	8 cut(s)	6, 39, 86, 165, 220, 396, 1127, 1854
FspBI	CTAG	5 cut(s)	660, 942, 1274, 1782, 1820
GlaI	GCGC	5 cut(s)	163, 218, 310, 345, 347
GluI	GCNGC	8 cut(s)	6, 39, 86, 165, 220, 396, 1127, 1854
GsaI	CCCAGC	1 cut(s)	762
GsuI	CTGGAG	1 cut(s)	42
HaeII	RGCGCY	1 cut(s)	312
HaeIII	GGCC	5 cut(s)	5, 356, 383, 553, 1793
HapII	CCGG	3 cut(s)	306, 384, 1249
HgaI	GACGC	3 cut(s)	355, 387, 1299
HhaI	GCGC	5 cut(s)	164, 219, 311, 346, 348
Hin1I	GRCGYC	4 cut(s)	309, 366, 398, 1291
Hin1II	CATG	8 cut(s)	649, 1015, 1040, 1124, 1169, 1298, 1472, 1557
Hin6I	GCGC	5 cut(s)	162, 217, 309, 344, 346
HinP1I	GCGC	5 cut(s)	162, 217, 309, 344, 346
HincII	GTYRAC	1 cut(s)	370
HindII	GTYRAC	1 cut(s)	370
HinfI	GANTC	6 cut(s)	586, 877, 1062, 1398, 1580, 1859
HpaII	CCGG	3 cut(s)	306, 384, 1249
HphI	GGTGA	9 cut(s)	24, 113, 310, 547, 637, 661, 755, 986, 1736
Hpy166II	GTNNAC	4 cut(s)	15, 370, 1110, 1289
Hpy8I	GTNNAC	4 cut(s)	15, 370, 1110, 1289
Hpy99I	CGWCG	3 cut(s)	293, 371, 403
HpyAV	CCTTC	6 cut(s)	216, 397, 502, 610, 712, 1606
HpyCH4III	ACNGT	7 cut(s)	467, 790, 802, 884, 1357, 1516, 1717
HpyCH4IV	ACGT	3 cut(s)	231, 864, 1207
HpyF3I	CTNAG	4 cut(s)	605, 714, 1283, 1415
HpySE526I	ACGT	3 cut(s)	231, 864, 1207
Hsp92I	GRCGYC	4 cut(s)	309, 366, 398, 1291
Hsp92II	CATG	8 cut(s)	649, 1015, 1040, 1124, 1169, 1298, 1472, 1557
HspAI	GCGC	5 cut(s)	162, 217, 309, 344, 346
KasI	GGCGCC	1 cut(s)	308
Ksp22I	TGATCA	2 cut(s)	867, 1384
LmnI	GCTCC	8 cut(s)	93, 154, 288, 595, 619, 642, 663, 1033
Lsp1109I	GCAGC	3 cut(s)	97, 1138, 1865
LweI	GCATC	8 cut(s)	56, 252, 691, 1107, 1396, 1519, 1657, 1806
MaeI	CTAG	5 cut(s)	660, 942, 1274, 1782, 1820
MaeII	ACGT	3 cut(s)	231, 864, 1207
MaeIII	GTNAC	7 cut(s)	119, 955, 1399, 1480, 1621, 1651, 1717
MboII	GAAGA	6 cut(s)	42, 45, 436, 622, 710, 1244
MfeI	CAATTG	1 cut(s)	947
MflI	RGATCY	2 cut(s)	710, 1148
MhlI	GDGCHC	2 cut(s)	17, 178
MlsI	TGGCCA	1 cut(s)	356
MluCI	AATT	9 cut(s)	448, 722, 812, 855, 947, 969, 1218, 1524, 1692
MluNI	TGGCCA	1 cut(s)	356
Mly113I	GGCGCC	1 cut(s)	309
MlyI	GAGTC	2 cut(s)	871, 1407
MmeI	TCCRAC	3 cut(s)	273, 1126, 1543
Mox20I	TGGCCA	1 cut(s)	356
Mph1103I	ATGCAT	1 cut(s)	1122
MroXI	GAANNNNTTC	2 cut(s)	1440, 1836
MscI	TGGCCA	1 cut(s)	356
MseI	TTAA	4 cut(s)	516, 837, 1019, 1518
MslI	CAYNNNNRTG	3 cut(s)	1115, 1170, 1185
Msp20I	TGGCCA	1 cut(s)	356
MspA1I	CMGCKG	2 cut(s)	8, 352
MspI	CCGG	3 cut(s)	306, 384, 1249
MspR9I	CCNGG	4 cut(s)	306, 385, 496, 852
MunI	CAATTG	1 cut(s)	947
Mva1269I	GAATGC	2 cut(s)	1197, 1651
MvaI	CCWGG	2 cut(s)	496, 852
MvnI	CGCG	5 cut(s)	162, 217, 288, 346, 434
NarI	GGCGCC	1 cut(s)	309
NciI	CCSGG	2 cut(s)	306, 385
NdeI	CATATG	2 cut(s)	591, 1213
NlaIII	CATG	8 cut(s)	649, 1015, 1040, 1124, 1169, 1298, 1472, 1557
NlaIV	GGNNCC	5 cut(s)	150, 310, 389, 1572, 1617
NmeAIII	GCCGAG	3 cut(s)	342, 579, 1051
NmuCI	GTSAC	4 cut(s)	119, 1399, 1651, 1717
NruI	TCGCGA	1 cut(s)	434
NsiI	ATGCAT	1 cut(s)	1122
NspI	RCATGY	3 cut(s)	1040, 1124, 1557
NspV	TTCGAA	1 cut(s)	414
PagI	TCATGA	2 cut(s)	1011, 1468
PaqCI	CACCTGC	1 cut(s)	613
PauI	GCGCGC	1 cut(s)	344
PceI	AGGCCT	1 cut(s)	1793
PcsI	WCGNNNNNNNCGW	2 cut(s)	375, 450
PctI	GAATGC	2 cut(s)	1197, 1651
PdmI	GAANNNNTTC	2 cut(s)	1440, 1836
PfeI	GAWTC	4 cut(s)	586, 1062, 1580, 1859
PinAI	ACCGGT	1 cut(s)	1248
PkrI	GCNGC	8 cut(s)	7, 40, 87, 166, 221, 397, 1128, 1855
PleI	GAGTC	2 cut(s)	871, 1406
PluTI	GGCGCC	1 cut(s)	312
PpsI	GAGTC	2 cut(s)	871, 1406
PsiI	TTATAA	1 cut(s)	752
Psp6I	CCWGG	2 cut(s)	494, 850
PspEI	GGTNACC	1 cut(s)	1480
PspFI	CCCAGC	1 cut(s)	758
PspGI	CCWGG	2 cut(s)	494, 850
PspN4I	GGNNCC	5 cut(s)	150, 310, 389, 1572, 1617
PspPI	GGNCC	2 cut(s)	1251, 1571
PstI	CTGCAG	1 cut(s)	1879
PstNI	CAGNNNCTG	2 cut(s)	1514, 1883
PsuI	RGATCY	2 cut(s)	710, 1148
PteI	GCGCGC	1 cut(s)	344
PvuII	CAGCTG	1 cut(s)	352
RruI	TCGCGA	1 cut(s)	434
RsaI	GTAC	5 cut(s)	542, 1041, 1109, 1210, 1637
RsaNI	GTAC	5 cut(s)	541, 1040, 1108, 1209, 1636
RseI	CAYNNNNRTG	3 cut(s)	1115, 1170, 1185
SalI	GTCGAC	1 cut(s)	368
SaqAI	TTAA	4 cut(s)	516, 837, 1019, 1518
SatI	GCNGC	8 cut(s)	6, 39, 86, 165, 220, 396, 1127, 1854
Sau96I	GGNCC	2 cut(s)	1251, 1571
ScaI	AGTACT	1 cut(s)	1637
SchI	GAGTC	2 cut(s)	871, 1407
ScrFI	CCNGG	4 cut(s)	306, 385, 496, 852
SduI	GDGCHC	2 cut(s)	17, 178
SfaNI	GCATC	8 cut(s)	56, 252, 691, 1107, 1396, 1519, 1657, 1806
SfcI	CTRYAG	3 cut(s)	1475, 1506, 1875
SfoI	GGCGCC	1 cut(s)	310
SfuI	TTCGAA	1 cut(s)	414
SinI	GGWCC	2 cut(s)	1251, 1571
SmiMI	CAYNNNNRTG	3 cut(s)	1115, 1170, 1185
SmlI	CTYRAG	2 cut(s)	1196, 1349
SmoI	CTYRAG	2 cut(s)	1196, 1349
Sse9I	AATT	9 cut(s)	448, 722, 812, 855, 947, 969, 1218, 1524, 1692
SseBI	AGGCCT	1 cut(s)	1793
SspDI	GGCGCC	1 cut(s)	308
SspMI	CTAG	5 cut(s)	660, 942, 1274, 1782, 1820
StuI	AGGCCT	1 cut(s)	1793
StyD4I	CCNGG	4 cut(s)	304, 383, 494, 850
StyI	CCWWGG	2 cut(s)	324, 1273
TaaI	ACNGT	7 cut(s)	467, 790, 802, 884, 1357, 1516, 1717
TaiI	ACGT	3 cut(s)	234, 867, 1210
TasI	AATT	9 cut(s)	448, 722, 812, 855, 947, 969, 1218, 1524, 1692
TatI	WGTACW	3 cut(s)	1039, 1107, 1635
TauI	GCSGC	5 cut(s)	8, 41, 167, 222, 398
TfiI	GAWTC	4 cut(s)	586, 1062, 1580, 1859
Tru1I	TTAA	4 cut(s)	516, 837, 1019, 1518
Tru9I	TTAA	4 cut(s)	516, 837, 1019, 1518
TscAI	CASTG	8 cut(s)	793, 887, 1117, 1408, 1581, 1726, 1753, 1884
TseFI	GTSAC	4 cut(s)	119, 1399, 1651, 1717
TseI	GCWGC	3 cut(s)	85, 1126, 1853
Tsp45I	GTSAC	4 cut(s)	119, 1399, 1651, 1717
TspGWI	ACGGA	2 cut(s)	86, 1753
TspRI	CASTG	8 cut(s)	793, 887, 1117, 1408, 1581, 1726, 1753, 1884
VneI	GTGCAC	1 cut(s)	13
VpaK11BI	GGWCC	2 cut(s)	1251, 1571
XapI	RAATTY	3 cut(s)	448, 722, 812
XceI	RCATGY	3 cut(s)	1040, 1124, 1557
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1455
XmaJI	CCTAGG	1 cut(s)	1273
XmiI	GTMKAC	1 cut(s)	369
XmnI	GAANNNNTTC	2 cut(s)	1440, 1836
XspI	CTAG	5 cut(s)	660, 942, 1274, 1782, 1820
ZrmI	AGTACT	1 cut(s)	1637
Zsp2I	ATGCAT	1 cut(s)	1122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.