Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003124

Physical Location & Seq

Genomic Coordinates Reverse (-)

28250061 .. 28256417

Gene Structure 6357 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G020680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGCCACTGTGTGCACTCCCCACACAATCTCACCCGCTGCCATTGTTTCTTCTTCCCGAGTCCGATGCTCTCACTCCCAATCGCAGCTCCTATTCCGTTCAAATTTCCTGAACCACCCCCGCCACCACAGCCCGAACCTCCCGATTTCGGCTACCAATCTCCGCGCCGCTCCCAAAGGACGAGTTCTCTGCTCGCAAAACCCTAACCCTACCCCCACGCGCCGCCCCGAATACGTCCCCAACCGCATCTCCGACCCGAACTATATCCGCATCTTCGACACCACGCTCCGCGACGGCGAGCAGGCCCCCGGCGCCTCCCTCACCTCCAAGGAGAAGCTCAACATTGCGCGCCAGCTGGCCAAGCTTGGCGTCGACATAATCGACGCCGGGTTCCCGGCTGCGTCGAAGGACGATTTCGTAGCCGTGAAGATGATCGCGAAAGAGGTCGGGAATTTCGTGGATAAAGACGGTTATGTCCCTGTGATCTGTGGGCTGTCCAGGTGCAATGAGAAGGATATTAAGTCGGCGTGGGAGGCGGTCAAGTATGCAAAGAGGCCGAGGATTCATACTTTCATTGCTACCAGTCCGATTCATATGGAGCATAAGCTGAGGAAGACGAAGGAGCAGGTGATCGAAATGGCGAGGAGTATGGTGAGCTATGCTAGGAGCTTGGGTTGCCAAGATGTCCAGTTTAGCCCGGAAGATGCCGGCAGATCTGAGAAGGAATTTCTATATCAGATTTTAGGTGAAGTTATAAAGGCCGGGGCAACAACTCTGAACATTCCTGACACTGTGGGTTATAACGTGCCAACTGAATTTGGTCAGTTGATTGCTGACATTAAATCTAATACCCCTGGAATTGATGACGTTATCATCTCGGCTCACTGCCACAATGATCTTGGACTTTCTACCGCCAACACTTTAGCGGGGGCAAGTAGAGGTGCTAGGCAATTGGAAGTAACAATCAATGGAATTGGTGAAAGGGCTGGTAATGCTTCATTAGAGGAGGTTGTCATGACCTTAAACTGCCGTGGGGAGCATGTATTTGGGGGGCTTTATACCGGAATAAATCCTCGGCATATTTATATGACAAGCAAGATGGTTTCAGAGTACACTGGAATGCATGTGCAGCAACACAAGGCTATTGTTGGAGCTAATGCTTTCTCACATGAAAGTGGTATCCATCAGGATGGAATGCTCAAGGACAAACGTACATATGAAATTATATCTCCTGAAGATATTGGGTATGACCGGTCCAGTGAAGCTGCCATTGTCCTAGGGAAACTTAGTGGACGCCATGCTTTGAAAAATCAGCTTCAACAGCTTGGTTATGAGCTTGATGATGTTCAACTTGAGACTGTATTCTGGCGCTTCAAAGGAGTAGCTGAACAGAAAAAGAGAGTCACTGATGCAGACCTCAGAGCATTGGTGTCAGATGAAGTTTTTCAGCCAGAAGTTGTCTGGAAGCTTCATGATCCACAGGAGACGTTGGGTGCTAGTGTCCCGTACGAACACCAGGATTGCGAACCAAATTCGATCAGAGATACAGATGCAGACAATCAAGCCCTGGAGGCAGAGAGAGAGAGAGAGAAGCGTCGAGAACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003852 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006551 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009098 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0019752 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046912 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00620 ko01100 ko01110 ko01210 ko01230 map00290 map00620 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

HMGL-like LeuA_dimer

Protein Analysis

535

Amino Acids

59.34

Weight (kDa)

6.59

Isoelectric Point (pI)

42.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMGL-like	PF00682	89 - 368	1e-88	HMGL-like
HCS_D2	PF22617	382 - 466	1.9e-28	Homocitrate synthase post-HMGL domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18500 AT1G74040 AT1G74040 AT1G74040
fragaria_vesca	FvH4_1g22410 FvH4_1g22460 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570
malus_domestica	MD01G1019700.v1.1 MD15G1332600.v1.1
prunus_persica	Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1
pyrus_communis	pycom01g04920 pycom15g29280
rosa_chinensis	RchiOBHm_Chr2g0116551 RchiOBHm_Chr2g0116561
rosa_laevigata	RLG00000018287 RLG00000018288
rosa_multiflora	Rmu_sc0001287.1_g000019 Rmu_sc0004191.1_g000022 Rmu_sc0004191.1_g000030
rosa_roxburghii	Rroxscaffold_2G00127060 Rroxscaffold_2G00127070
rosa_rugosa	Rorug02G0207600
rosa_samantha	Rh2AG262500 Rh2AG262600 Rh2BG274100 Rh2BG274400 Rh2DG270700 Rh2DG270800 Rh2DG289200 Rh2DG289300
rosa_wichuraiana	Rw2G020670 Rw2G020680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	755, 801
AarI	CACCTGC	1 cut(s)	616
AasI	GACNNNNNNGTC	1 cut(s)	473
Acc36I	ACCTGC	1 cut(s)	616
AccB1I	GGYRCC	1 cut(s)	311
AccBSI	CCGCTC	1 cut(s)	170
AccI	GTMKAC	1 cut(s)	372
AccII	CGCG	5 cut(s)	165, 220, 291, 349, 437
AclWI	GGATC	1 cut(s)	1469
AcoI	YGGCCR	2 cut(s)	3, 357
AcsI	RAATTY	5 cut(s)	103, 451, 725, 815, 1531
AcuI	CTGAAG	1 cut(s)	1254
AcyI	GRCGYC	4 cut(s)	312, 369, 384, 1294
AdeI	CACNNNGTG	1 cut(s)	12
AfaI	GTAC	3 cut(s)	1112, 1213, 1508
AfiI	CCNNNNNNNGG	1 cut(s)	148
AgeI	ACCGGT	1 cut(s)	1251
AgsI	TTSAA	5 cut(s)	102, 1306, 1319, 1349, 1375
AjnI	CCWGG	4 cut(s)	497, 853, 1515, 1566
Alw21I	GWGCWC	1 cut(s)	17
Alw26I	GTCTC	2 cut(s)	1349, 1478
Alw44I	GTGCAC	1 cut(s)	13
AlwI	GGATC	1 cut(s)	1469
Ama87I	CYCGRG	1 cut(s)	57
AoxI	GGCC	5 cut(s)	3, 303, 357, 554, 759
ApaLI	GTGCAC	1 cut(s)	13
ApeKI	GCWGC	5 cut(s)	38, 85, 398, 1129, 1265
ApoI	RAATTY	5 cut(s)	103, 451, 725, 815, 1531
AsiGI	ACCGGT	1 cut(s)	1251
Asp700I	GAANNNNTTC	1 cut(s)	1443
AspA2I	CCTAGG	1 cut(s)	1276
AspLEI	GCGC	6 cut(s)	167, 222, 314, 349, 351, 1371
AspS9I	GGNCC	2 cut(s)	304, 1254
AsuC2I	CCSGG	5 cut(s)	309, 388, 395, 698, 763
AsuHPI	GGTGA	6 cut(s)	24, 313, 640, 664, 758, 989
AvaI	CYCGRG	1 cut(s)	57
AvaII	GGWCC	1 cut(s)	1254
AvrII	CCTAGG	1 cut(s)	1276
BaeGI	GKGCMC	1 cut(s)	17
BalI	TGGCCA	2 cut(s)	5, 359
BanI	GGYRCC	1 cut(s)	311
BbsI	GAAGAC	1 cut(s)	620
Bbv12I	GWGCWC	1 cut(s)	17
BbvCI	CCTCAGC	1 cut(s)	608
BbvI	GCAGC	5 cut(s)	25, 97, 385, 1141, 1252
BccI	CCATC	3 cut(s)	1093, 1184, 1191
BceAI	ACGGC	3 cut(s)	310, 407, 1014
BcgI	CGANNNNNNTGC	4 cut(s)	48, 82, 171, 205
BciT130I	CCWGG	4 cut(s)	499, 855, 1517, 1568
BciVI	GTATCC	1 cut(s)	1190
BcnI	CCSGG	5 cut(s)	309, 388, 395, 698, 763
BcoDI	GTCTC	2 cut(s)	1349, 1478
BfaI	CTAG	4 cut(s)	663, 945, 1277, 1497
BfoI	RGCGCY	2 cut(s)	315, 1372
BfuAI	ACCTGC	1 cut(s)	616
BfuI	GTATCC	1 cut(s)	1190
BglI	GCCNNNNNGGC	1 cut(s)	1571
BglII	AGATCT	1 cut(s)	713
BisI	GCNGC	7 cut(s)	39, 86, 168, 223, 399, 1130, 1266
BlnI	CCTAGG	1 cut(s)	1276
BlsI	GCNGC	7 cut(s)	40, 87, 169, 224, 400, 1131, 1267
Bme1390I	CCNGG	9 cut(s)	309, 388, 395, 499, 698, 763, 855, 1517, 1568
Bme18I	GGWCC	1 cut(s)	1254
BmeT110I	CYCGRG	1 cut(s)	57
BmgT120I	GGNCC	2 cut(s)	304, 1254
BmiI	GGNNCC	3 cut(s)	306, 313, 392
BmrFI	CCNGG	9 cut(s)	309, 388, 395, 499, 698, 763, 855, 1517, 1568
BmsI	GCATC	6 cut(s)	56, 255, 279, 694, 1399, 1540
BpiI	GAAGAC	1 cut(s)	620
BpmI	CTGGAG	1 cut(s)	1589
Bpu10I	CCTNAGC	1 cut(s)	608
BpuEI	CTTGAG	2 cut(s)	1184, 1373
BpuMI	CCSGG	5 cut(s)	309, 388, 395, 698, 763
BsaHI	GRCGYC	4 cut(s)	312, 369, 384, 1294
BsaJI	CCNNGG	9 cut(s)	307, 327, 557, 762, 853, 1030, 1073, 1276, 1566
BsaWI	WCCGGW	2 cut(s)	1061, 1251
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 658, 688
Bsc4I	CCNNNNNNNGG	1 cut(s)	148
Bse118I	RCCGGY	2 cut(s)	707, 1251
Bse1I	ACTGG	4 cut(s)	582, 688, 1120, 1257
Bse3DI	GCAATG	3 cut(s)	342, 511, 573
BseBI	CCWGG	4 cut(s)	499, 855, 1517, 1568
BseDI	CCNNGG	9 cut(s)	307, 327, 557, 762, 853, 1030, 1073, 1276, 1566
BseGI	GGATG	1 cut(s)	1195
BseLI	CCNNNNNNNGG	1 cut(s)	148
BseMI	GCAATG	3 cut(s)	342, 511, 573
BseMII	CTCAG	3 cut(s)	599, 708, 1432
BseNI	ACTGG	4 cut(s)	582, 688, 1120, 1257
BsePI	GCGCGC	1 cut(s)	347
BseRI	GAGGAG	2 cut(s)	658, 1019
BseSI	GKGCMC	1 cut(s)	17
BseXI	GCAGC	5 cut(s)	25, 97, 385, 1141, 1252
BsgI	GTGCAG	1 cut(s)	1148
Bsh1236I	CGCG	5 cut(s)	165, 220, 291, 349, 437
BshFI	GGCC	5 cut(s)	5, 305, 359, 556, 761
BshNI	GGYRCC	1 cut(s)	311
BshTI	ACCGGT	1 cut(s)	1251
BsiHKAI	GWGCWC	1 cut(s)	17
BsiHKCI	CYCGRG	1 cut(s)	57
BsiSI	CCGG	8 cut(s)	309, 387, 395, 698, 708, 762, 1062, 1252
BsiWI	CGTACG	1 cut(s)	1506
BslFI	GGGAC	3 cut(s)	221, 461, 1487
BslI	CCNNNNNNNGG	1 cut(s)	148
BsmAI	GTCTC	2 cut(s)	1349, 1478
BsmBI	CGTCTC	1 cut(s)	1478
BsmFI	GGGAC	3 cut(s)	221, 461, 1487
BsmI	GAATGC	2 cut(s)	1125, 1200
BsnI	GGCC	5 cut(s)	5, 305, 359, 556, 761
BsoBI	CYCGRG	1 cut(s)	57
Bsp1286I	GDGCHC	1 cut(s)	17
Bsp143I	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
Bsp68I	TCGCGA	1 cut(s)	437
BspANI	GGCC	5 cut(s)	5, 305, 359, 556, 761
BspCNI	CTCAG	3 cut(s)	600, 709, 1431
BspFNI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BspHI	TCATGA	2 cut(s)	1014, 1471
BspLI	GGNNCC	3 cut(s)	306, 313, 392
BspMI	ACCTGC	1 cut(s)	616
BspPI	GGATC	1 cut(s)	1469
BspT107I	GGYRCC	1 cut(s)	311
BsrBI	CCGCTC	1 cut(s)	170
BsrDI	GCAATG	3 cut(s)	342, 511, 573
BsrFI	RCCGGY	2 cut(s)	707, 1251
BsrI	ACTGG	4 cut(s)	582, 688, 1120, 1257
BssAI	RCCGGY	2 cut(s)	707, 1251
BssECI	CCNNGG	9 cut(s)	307, 327, 557, 762, 853, 1030, 1073, 1276, 1566
BssHII	GCGCGC	1 cut(s)	347
BssMI	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
BssNI	GRCGYC	4 cut(s)	312, 369, 384, 1294
BssT1I	CCWWGG	2 cut(s)	327, 1276
Bst2UI	CCWGG	4 cut(s)	499, 855, 1517, 1568
Bst4CI	ACNGT	4 cut(s)	10, 470, 793, 1360
BstACI	GRCGYC	4 cut(s)	312, 369, 384, 1294
BstC8I	GCNNGC	7 cut(s)	194, 299, 303, 349, 353, 357, 709
BstDEI	CTNAG	4 cut(s)	608, 717, 1286, 1418
BstDSI	CCRYGG	1 cut(s)	1030
BstF5I	GGATG	1 cut(s)	1195
BstFNI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BstH2I	RGCGCY	2 cut(s)	315, 1372
BstHHI	GCGC	6 cut(s)	167, 222, 314, 349, 351, 1371
BstKTI	GATC	7 cut(s)	435, 486, 633, 716, 898, 1477, 1539
BstMAI	GTCTC	2 cut(s)	1349, 1478
BstMBI	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
BstMWI	GCNNNNNNNGC	9 cut(s)	129, 311, 361, 533, 675, 992, 1129, 1321, 1571
BstNI	CCWGG	4 cut(s)	499, 855, 1517, 1568
BstNSI	RCATGY	2 cut(s)	1043, 1127
BstSCI	CCNGG	9 cut(s)	307, 386, 393, 497, 696, 761, 853, 1515, 1566
BstSLI	GKGCMC	1 cut(s)	17
BstUI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BstV1I	GCAGC	5 cut(s)	25, 97, 385, 1141, 1252
BstV2I	GAAGAC	1 cut(s)	620
BstX2I	RGATCY	1 cut(s)	713
BstXI	CCANNNNNNTGG	1 cut(s)	1190
BstYI	RGATCY	1 cut(s)	713
BsuI	GTATCC	1 cut(s)	1190
BsuRI	GGCC	5 cut(s)	5, 305, 359, 556, 761
BtgI	CCRYGG	1 cut(s)	1030
BtsCI	GGATG	1 cut(s)	1195
BtsI	GCAGTG	1 cut(s)	883
BtsIMutI	CAGTG	6 cut(s)	6, 789, 883, 1113, 1264, 1404
BtuMI	TCGCGA	1 cut(s)	437
BveI	ACCTGC	1 cut(s)	616
Cac8I	GCNNGC	7 cut(s)	194, 299, 303, 349, 353, 357, 709
CciI	TCATGA	2 cut(s)	1014, 1471
CfoI	GCGC	6 cut(s)	167, 222, 314, 349, 351, 1371
Cfr10I	RCCGGY	2 cut(s)	707, 1251
Cfr13I	GGNCC	2 cut(s)	304, 1254
CseI	GACGC	5 cut(s)	358, 390, 392, 1302, 1583
Csp6I	GTAC	3 cut(s)	1111, 1212, 1507
CspAI	ACCGGT	1 cut(s)	1251
CviAII	CATG	6 cut(s)	1015, 1040, 1124, 1169, 1298, 1472
CviQI	GTAC	3 cut(s)	1111, 1212, 1507
DdeI	CTNAG	4 cut(s)	608, 717, 1286, 1418
DinI	GGCGCC	1 cut(s)	313
DpnI	GATC	7 cut(s)	434, 485, 632, 715, 897, 1476, 1538
DpnII	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
DraIII	CACNNNGTG	1 cut(s)	12
DrdI	GACNNNNNNGTC	1 cut(s)	473
DseDI	GACNNNNNNGTC	1 cut(s)	473
EaeI	YGGCCR	2 cut(s)	3, 357
Eco130I	CCWWGG	2 cut(s)	327, 1276
Eco47I	GGWCC	1 cut(s)	1254
Eco57I	CTGAAG	1 cut(s)	1254
Eco88I	CYCGRG	1 cut(s)	57
EcoO109I	RGGNCCY	1 cut(s)	304
EcoRII	CCWGG	4 cut(s)	497, 853, 1515, 1566
EcoT14I	CCWWGG	2 cut(s)	327, 1276
EcoT22I	ATGCAT	1 cut(s)	1125
EgeI	GGCGCC	1 cut(s)	313
EheI	GGCGCC	1 cut(s)	313
ErhI	CCWWGG	2 cut(s)	327, 1276
Esp3I	CGTCTC	1 cut(s)	1478
FaeI	CATG	6 cut(s)	1018, 1043, 1127, 1172, 1301, 1475
FaqI	GGGAC	3 cut(s)	221, 461, 1487
FatI	CATG	6 cut(s)	1014, 1039, 1123, 1168, 1297, 1471
FauI	CCCGC	3 cut(s)	43, 128, 919
FauNDI	CATATG	2 cut(s)	594, 1216
FblI	GTMKAC	1 cut(s)	372
Fnu4HI	GCNGC	7 cut(s)	39, 86, 168, 223, 399, 1130, 1266
FokI	GGATG	1 cut(s)	1202
Fsp4HI	GCNGC	7 cut(s)	39, 86, 168, 223, 399, 1130, 1266
FspBI	CTAG	4 cut(s)	663, 945, 1277, 1497
GlaI	GCGC	6 cut(s)	166, 221, 313, 348, 350, 1370
GluI	GCNGC	7 cut(s)	39, 86, 168, 223, 399, 1130, 1266
GsuI	CTGGAG	1 cut(s)	1589
HaeII	RGCGCY	2 cut(s)	315, 1372
HaeIII	GGCC	5 cut(s)	5, 305, 359, 556, 761
HapII	CCGG	8 cut(s)	309, 387, 395, 698, 708, 762, 1062, 1252
HgaI	GACGC	5 cut(s)	358, 390, 392, 1302, 1583
HhaI	GCGC	6 cut(s)	167, 222, 314, 349, 351, 1371
Hin1I	GRCGYC	4 cut(s)	312, 369, 384, 1294
Hin1II	CATG	6 cut(s)	1018, 1043, 1127, 1172, 1301, 1475
Hin6I	GCGC	6 cut(s)	165, 220, 312, 347, 349, 1369
HinP1I	GCGC	6 cut(s)	165, 220, 312, 347, 349, 1369
HincII	GTYRAC	1 cut(s)	373
HindII	GTYRAC	1 cut(s)	373
HindIII	AAGCTT	2 cut(s)	362, 1466
HinfI	GANTC	4 cut(s)	60, 562, 589, 1401
HpaII	CCGG	8 cut(s)	309, 387, 395, 698, 708, 762, 1062, 1252
HphI	GGTGA	6 cut(s)	24, 313, 640, 664, 758, 989
Hpy166II	GTNNAC	4 cut(s)	15, 373, 1113, 1292
Hpy8I	GTNNAC	4 cut(s)	15, 373, 1113, 1292
Hpy99I	CGWCG	5 cut(s)	296, 374, 386, 406, 1599
HpyAV	CCTTC	4 cut(s)	400, 505, 613, 715
HpyCH4III	ACNGT	4 cut(s)	10, 470, 793, 1360
HpyCH4IV	ACGT	5 cut(s)	234, 804, 867, 1210, 1487
HpyCH4V	TGCA	7 cut(s)	15, 504, 548, 1123, 1129, 1412, 1553
HpyF10VI	GCNNNNNNNGC	9 cut(s)	129, 311, 361, 533, 675, 992, 1129, 1321, 1571
HpyF3I	CTNAG	4 cut(s)	608, 717, 1286, 1418
HpySE526I	ACGT	5 cut(s)	234, 804, 867, 1210, 1487
Hsp92I	GRCGYC	4 cut(s)	312, 369, 384, 1294
Hsp92II	CATG	6 cut(s)	1018, 1043, 1127, 1172, 1301, 1475
HspAI	GCGC	6 cut(s)	165, 220, 312, 347, 349, 1369
KasI	GGCGCC	1 cut(s)	311
KroI	GCCGGC	1 cut(s)	707
KroNI	GCCGGC	1 cut(s)	709
Kzo9I	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
LmnI	GCTCC	8 cut(s)	93, 175, 291, 598, 622, 666, 1036, 1151
Lsp1109I	GCAGC	5 cut(s)	25, 97, 385, 1141, 1252
LweI	GCATC	6 cut(s)	56, 255, 279, 694, 1399, 1540
MaeI	CTAG	4 cut(s)	663, 945, 1277, 1497
MaeII	ACGT	5 cut(s)	234, 804, 867, 1210, 1487
MaeIII	GTNAC	2 cut(s)	958, 1402
MalI	GATC	7 cut(s)	434, 485, 632, 715, 897, 1476, 1538
MbiI	CCGCTC	1 cut(s)	170
MboI	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
MboII	GAAGA	7 cut(s)	42, 45, 265, 439, 625, 713, 1247
MfeI	CAATTG	1 cut(s)	950
MflI	RGATCY	1 cut(s)	713
MhlI	GDGCHC	1 cut(s)	17
MlsI	TGGCCA	2 cut(s)	5, 359
MluCI	AATT	9 cut(s)	103, 451, 725, 815, 858, 950, 972, 1221, 1531
MluNI	TGGCCA	2 cut(s)	5, 359
Mly113I	GGCGCC	1 cut(s)	312
MlyI	GAGTC	2 cut(s)	69, 1410
MmeI	TCCRAC	2 cut(s)	276, 1129
Mox20I	TGGCCA	2 cut(s)	5, 359
Mph1103I	ATGCAT	1 cut(s)	1125
MroNI	GCCGGC	1 cut(s)	707
MroXI	GAANNNNTTC	1 cut(s)	1443
MscI	TGGCCA	2 cut(s)	5, 359
MseI	TTAA	4 cut(s)	519, 840, 1022, 1606
MslI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
Msp20I	TGGCCA	2 cut(s)	5, 359
MspA1I	CMGCKG	2 cut(s)	38, 355
MspI	CCGG	8 cut(s)	309, 387, 395, 698, 708, 762, 1062, 1252
MspR9I	CCNGG	9 cut(s)	309, 388, 395, 499, 698, 763, 855, 1517, 1568
MunI	CAATTG	1 cut(s)	950
Mva1269I	GAATGC	2 cut(s)	1125, 1200
MvaI	CCWGG	4 cut(s)	499, 855, 1517, 1568
MvnI	CGCG	5 cut(s)	165, 220, 291, 349, 437
MwoI	GCNNNNNNNGC	9 cut(s)	129, 311, 361, 533, 675, 992, 1129, 1321, 1571
NaeI	GCCGGC	1 cut(s)	709
NarI	GGCGCC	1 cut(s)	312
NciI	CCSGG	5 cut(s)	309, 388, 395, 698, 763
NdeI	CATATG	2 cut(s)	594, 1216
NdeII	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
NgoMIV	GCCGGC	1 cut(s)	707
NlaIII	CATG	6 cut(s)	1018, 1043, 1127, 1172, 1301, 1475
NlaIV	GGNNCC	3 cut(s)	306, 313, 392
NmeAIII	GCCGAG	3 cut(s)	582, 857, 1054
NmuCI	GTSAC	1 cut(s)	1402
NruI	TCGCGA	1 cut(s)	437
NsiI	ATGCAT	1 cut(s)	1125
NspI	RCATGY	2 cut(s)	1043, 1127
PagI	TCATGA	2 cut(s)	1014, 1471
PaqCI	CACCTGC	1 cut(s)	616
PauI	GCGCGC	1 cut(s)	347
PcsI	WCGNNNNNNNCGW	2 cut(s)	378, 453
PctI	GAATGC	2 cut(s)	1125, 1200
PdiI	GCCGGC	1 cut(s)	709
PdmI	GAANNNNTTC	1 cut(s)	1443
PfeI	GAWTC	2 cut(s)	562, 589
Pfl23II	CGTACG	1 cut(s)	1506
PinAI	ACCGGT	1 cut(s)	1251
PkrI	GCNGC	7 cut(s)	40, 87, 169, 224, 400, 1131, 1267
PleI	GAGTC	2 cut(s)	68, 1409
PluTI	GGCGCC	1 cut(s)	315
PpsI	GAGTC	2 cut(s)	68, 1409
PsiI	TTATAA	2 cut(s)	755, 801
Psp6I	CCWGG	4 cut(s)	497, 853, 1515, 1566
PspGI	CCWGG	4 cut(s)	497, 853, 1515, 1566
PspLI	CGTACG	1 cut(s)	1506
PspN4I	GGNNCC	3 cut(s)	306, 313, 392
PspPI	GGNCC	2 cut(s)	304, 1254
PsuI	RGATCY	1 cut(s)	713
PteI	GCGCGC	1 cut(s)	347
PvuII	CAGCTG	1 cut(s)	355
RruI	TCGCGA	1 cut(s)	437
RsaI	GTAC	3 cut(s)	1112, 1213, 1508
RsaNI	GTAC	3 cut(s)	1111, 1212, 1507
RseI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
SalI	GTCGAC	1 cut(s)	371
SaqAI	TTAA	4 cut(s)	519, 840, 1022, 1606
SatI	GCNGC	7 cut(s)	39, 86, 168, 223, 399, 1130, 1266
Sau3AI	GATC	7 cut(s)	432, 483, 630, 713, 895, 1474, 1536
Sau96I	GGNCC	2 cut(s)	304, 1254
SchI	GAGTC	2 cut(s)	69, 1410
ScrFI	CCNGG	9 cut(s)	309, 388, 395, 499, 698, 763, 855, 1517, 1568
SduI	GDGCHC	1 cut(s)	17
SfaNI	GCATC	6 cut(s)	56, 255, 279, 694, 1399, 1540
SfoI	GGCGCC	1 cut(s)	313
SinI	GGWCC	1 cut(s)	1254
SmiMI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
SmlI	CTYRAG	2 cut(s)	1199, 1352
SmoI	CTYRAG	2 cut(s)	1199, 1352
Sse9I	AATT	9 cut(s)	103, 451, 725, 815, 858, 950, 972, 1221, 1531
SspDI	GGCGCC	1 cut(s)	311
SspMI	CTAG	4 cut(s)	663, 945, 1277, 1497
StyD4I	CCNGG	9 cut(s)	307, 386, 393, 497, 696, 761, 853, 1515, 1566
StyI	CCWWGG	2 cut(s)	327, 1276
TaaI	ACNGT	4 cut(s)	10, 470, 793, 1360
TaiI	ACGT	5 cut(s)	237, 807, 870, 1213, 1490
TaqI	TCGA	7 cut(s)	276, 372, 381, 404, 633, 1535, 1597
TasI	AATT	9 cut(s)	103, 451, 725, 815, 858, 950, 972, 1221, 1531
TatI	WGTACW	1 cut(s)	1110
TauI	GCSGC	2 cut(s)	170, 225
TfiI	GAWTC	2 cut(s)	562, 589
Tru1I	TTAA	4 cut(s)	519, 840, 1022, 1606
Tru9I	TTAA	4 cut(s)	519, 840, 1022, 1606
TscAI	CASTG	6 cut(s)	13, 796, 890, 1120, 1264, 1411
TseFI	GTSAC	1 cut(s)	1402
TseI	GCWGC	5 cut(s)	38, 85, 398, 1129, 1265
Tsp45I	GTSAC	1 cut(s)	1402
TspDTI	ATGAA	8 cut(s)	554, 562, 581, 987, 1185, 1233, 1452, 1460
TspGWI	ACGGA	1 cut(s)	86
TspRI	CASTG	6 cut(s)	13, 796, 890, 1120, 1264, 1411
VneI	GTGCAC	1 cut(s)	13
VpaK11BI	GGWCC	1 cut(s)	1254
XapI	RAATTY	5 cut(s)	103, 451, 725, 815, 1531
XceI	RCATGY	2 cut(s)	1043, 1127
XcmI	CCANNNNNNNNNTGG	2 cut(s)	896, 1458
XmaJI	CCTAGG	1 cut(s)	1276
XmiI	GTMKAC	1 cut(s)	372
XmnI	GAANNNNTTC	1 cut(s)	1443
XspI	CTAG	4 cut(s)	663, 945, 1277, 1497
Zsp2I	ATGCAT	1 cut(s)	1125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.