Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0003124

Physical Location & Seq

Genomic Coordinates Reverse (-)

30300333 .. 30306155

Gene Structure 5823 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG262500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGGCCGCTGTGTGCACTCCCCACACAATCTCAACCGCCGCCATAGTTTCTTCTTCTTCTCCAGTCCGATGCTCTCACTCCCAATCGCAGCTCCTATTCAGTTCAAATCTCCCGAACCGCTGTCACCACAGCCCGAGCCTCCCGATTTCGGCTCCCAATCTCCGCGCCGCTACCAGAGGACGAGTTCTCTGCTCACAAAACCCTAACCCTGCCCCCACGCGCCGCCCCGACTACGTCCCCAACCGCATCTCCGACCCGAACTATGTCCGCATATTCGACACCACGCTCCGCGACGGCGAGCAGTCCCCCGGCGCCTCGCTCACCTCCAAGGAGAAGCTCGACATTGCGCGCCAGCTGGCCAAGCTCGGCGTCGACATAATCGAGGCCGGGTTCCCGGCGGCGTCGAAGGACGACTTCGAAGCCGTGAAGATGATCGCGAAAGAGGTCGGGAATTTCGTGGATAAAGACGGTTATGTCCCTGTGATCTGTGGGCTGTCCAGGTGCAATGAGAAGGATATTAAGACGGCGTGGGAGGCGGTCAAGTACGCAAAGAGGCCGAGGATTCATACTTTCATTGCTACCAGTCCGATTCATATGGAGCATAAGCTGAGGAAGACGAAGGAGCAGGTGATCGAGATTGCCAGGAGCATGGTGAGCTACGCTAGGAGCTTGGGTTGCCAAGATGTCGAGTTTAGCCCTGAAGATGCTGGCAGATCTGAGAAGGAATTTCTATATCAGATTTTAGGTGAAGTTATAAAGGCTGGGGCAACGACTCTGAACATTCCTGACACTGTGGGTTATACCGTGCCAACTGAATTTGGTCAGTTGATTGCTGACATTAAATCTAATACCCCTGGAATTGATAACGTGATCATCTCAACTCACTGCCAAAATGATCTTGGACTTTCTACCGCCAACACTTTAGCGGGGGCAAGTAGAGGTGCTAGGCAATTGGAAGTAACAATCAATGGAATTGGTGAAAGGGCTGGTAATGCTTCATTAGAGGAGGTTGTCATGACCTTAAACTGCCGTGGGGAGCATGTACTTGGGGGGCTTTATACTGGAATCAATCCTCGGCATATTTATATGACGAGCAAGATGGTTTCAGAGTACACTGGGATGCATGTGCAGCCACACAAGGCTATTGTTGGATCTAATGCTTTTGCACATGAAAGTGGTATCCATCAGGATGGAATGCTCAAGGACAAACGTACATATGAAATTATATGTCCTGAAGATATTGGGTATGACCGGTCCAATGAAGCTGGCATTGTCCTAGGGAAACTTAGTGGACGCCATGCTGTGAAAAAGCAACTTGAACAGCTTGGTTATGAGCTTGATGATGTTCAACTTGAGACTGTATTCTGGCGTTTCAAAGGAGTAGCTGATCAGAAAAAGAGAGTCACTGATGCAGACCTCAGAGCATTGGTGTCAGATGAAGTTTTTCAGCCAGAAGTTGTCTGGAAACTTCATGATCTACAGGTTACCTGTGGAACTCTTGGTCTTTCTACAGCAACTGTTAAACTAATTGATGCTGATGGGAGAGAGCATATTGCATGTTCAGTTGGAACGGGTCCAGTGGATTCAGCTTACAAGGCTGTTGATCTCATTGTGAAGGAACCAGTAACACTTGTTGAGTACTCTATGAATGCGGTCACAGAAGGAATAGATGCAATAGCCACTACTCGTGTTGCAATTCGAGTTGAAAATAGTGATACGGTCACCGGTGAGATATCTCAACGGACATTCAGTGGGATTGGGGCAGGAATGGACATTGTTGTTTCTAGTGTCAAGGCCTACATTGGTGCATTGAACAAGATGCTAGGTTTCAAGGAAAGGTTTCCATTAGAGGTTGCAGCAGAATCAGCAGAACGAACTGCAGTGTCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003852 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006551 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009098 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0019752 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046912 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00620 ko01100 ko01110 ko01210 ko01230 map00290 map00620 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

HMGL-like LeuA_dimer

Protein Analysis

630

Amino Acids

68.41

Weight (kDa)

6.22

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMGL-like	PF00682	89 - 368	3.9e-89	HMGL-like
HCS_D2	PF22617	382 - 466	1.8e-28	Homocitrate synthase post-HMGL domain-like
LeuA_dimer	PF08502	467 - 608	3.5e-32	LeuA allosteric (dimerisation) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18500 AT1G74040 AT1G74040 AT1G74040
fragaria_vesca	FvH4_1g22410 FvH4_1g22460 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570
malus_domestica	MD01G1019700.v1.1 MD15G1332600.v1.1
prunus_persica	Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1
pyrus_communis	pycom01g04920 pycom15g29280
rosa_chinensis	RchiOBHm_Chr2g0116551 RchiOBHm_Chr2g0116561
rosa_laevigata	RLG00000018287 RLG00000018288
rosa_multiflora	Rmu_sc0001287.1_g000019 Rmu_sc0004191.1_g000022 Rmu_sc0004191.1_g000030
rosa_roxburghii	Rroxscaffold_2G00127060 Rroxscaffold_2G00127070
rosa_rugosa	Rorug02G0207600
rosa_samantha	Rh2AG262500 Rh2AG262600 Rh2BG274100 Rh2BG274400 Rh2DG270700 Rh2DG270800 Rh2DG289200 Rh2DG289300
rosa_wichuraiana	Rw2G020670 Rw2G020680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	755
AarI	CACCTGC	1 cut(s)	616
AasI	GACNNNNNNGTC	1 cut(s)	473
Acc36I	ACCTGC	1 cut(s)	616
AccB1I	GGYRCC	1 cut(s)	311
AccI	GTMKAC	1 cut(s)	372
AccII	CGCG	5 cut(s)	165, 220, 291, 349, 437
AclWI	GGATC	1 cut(s)	1159
AcoI	YGGCCR	2 cut(s)	3, 357
AcsI	RAATTY	3 cut(s)	451, 725, 815
AcuI	CTGAAG	2 cut(s)	720, 1254
AcyI	GRCGYC	4 cut(s)	312, 369, 401, 1294
AfaI	GTAC	5 cut(s)	545, 1044, 1112, 1213, 1640
AfiI	CCNNNNNNNGG	1 cut(s)	148
AgeI	ACCGGT	2 cut(s)	1251, 1724
AgsI	TTSAA	7 cut(s)	105, 1319, 1349, 1375, 1706, 1813, 1831
AjnI	CCWGG	3 cut(s)	497, 641, 853
Alw21I	GWGCWC	1 cut(s)	17
Alw26I	GTCTC	1 cut(s)	1349
Alw44I	GTGCAC	1 cut(s)	13
AlwI	GGATC	1 cut(s)	1159
AlwNI	CAGNNNCTG	2 cut(s)	1517, 1886
Ama87I	CYCGRG	1 cut(s)	133
AoxI	GGCC	5 cut(s)	3, 357, 384, 554, 1794
ApaLI	GTGCAC	1 cut(s)	13
ApeKI	GCWGC	3 cut(s)	88, 1129, 1856
ApoI	RAATTY	3 cut(s)	451, 725, 815
AsiGI	ACCGGT	2 cut(s)	1251, 1724
Asp700I	GAANNNNTTC	2 cut(s)	1443, 1839
AspA2I	CCTAGG	1 cut(s)	1276
AspLEI	GCGC	5 cut(s)	167, 222, 314, 349, 351
AspS9I	GGNCC	2 cut(s)	1254, 1574
AsuC2I	CCSGG	3 cut(s)	309, 388, 395
AsuHPI	GGTGA	8 cut(s)	116, 313, 640, 664, 758, 989, 1714, 1739
AsuII	TTCGAA	1 cut(s)	417
AvaI	CYCGRG	1 cut(s)	133
AvaII	GGWCC	2 cut(s)	1254, 1574
AvrII	CCTAGG	1 cut(s)	1276
BaeGI	GKGCMC	1 cut(s)	17
BalI	TGGCCA	1 cut(s)	359
BanI	GGYRCC	1 cut(s)	311
BauI	CACGAG	1 cut(s)	1686
BbsI	GAAGAC	1 cut(s)	620
Bbv12I	GWGCWC	1 cut(s)	17
BbvCI	CCTCAGC	1 cut(s)	608
BbvI	GCAGC	3 cut(s)	100, 1141, 1868
BccI	CCATC	4 cut(s)	1093, 1184, 1191, 1532
BceAI	ACGGC	4 cut(s)	310, 407, 540, 1014
BcgI	CGANNNNNNTGC	2 cut(s)	171, 205
BciT130I	CCWGG	3 cut(s)	499, 643, 855
BciVI	GTATCC	1 cut(s)	1190
BclI	TGATCA	2 cut(s)	870, 1387
BcnI	CCSGG	3 cut(s)	309, 388, 395
BcoDI	GTCTC	1 cut(s)	1349
BfaI	CTAG	5 cut(s)	663, 945, 1277, 1785, 1823
BfmI	CTRYAG	3 cut(s)	1478, 1509, 1878
BfoI	RGCGCY	1 cut(s)	315
BfuAI	ACCTGC	1 cut(s)	616
BfuI	GTATCC	1 cut(s)	1190
BglII	AGATCT	1 cut(s)	713
BisI	GCNGC	8 cut(s)	6, 39, 89, 168, 223, 399, 1130, 1857
BlnI	CCTAGG	1 cut(s)	1276
BlsI	GCNGC	8 cut(s)	7, 40, 90, 169, 224, 400, 1131, 1858
BmcAI	AGTACT	1 cut(s)	1640
Bme1390I	CCNGG	6 cut(s)	309, 388, 395, 499, 643, 855
Bme18I	GGWCC	2 cut(s)	1254, 1574
BmeT110I	CYCGRG	1 cut(s)	133
BmgT120I	GGNCC	2 cut(s)	1254, 1574
BmiI	GGNNCC	5 cut(s)	153, 313, 392, 1575, 1620
BmrFI	CCNGG	6 cut(s)	309, 388, 395, 499, 643, 855
BmrI	ACTGGG	1 cut(s)	1125
BmsI	GCATC	8 cut(s)	59, 255, 694, 1110, 1399, 1522, 1660, 1809
BmuI	ACTGGG	1 cut(s)	1125
BpiI	GAAGAC	1 cut(s)	620
BpmI	CTGGAG	1 cut(s)	45
Bpu10I	CCTNAGC	1 cut(s)	608
Bpu14I	TTCGAA	1 cut(s)	417
BpuEI	CTTGAG	2 cut(s)	1184, 1373
BpuMI	CCSGG	3 cut(s)	309, 388, 395
BsaHI	GRCGYC	4 cut(s)	312, 369, 401, 1294
BsaJI	CCNNGG	7 cut(s)	307, 327, 557, 853, 1030, 1073, 1276
BsaWI	WCCGGW	2 cut(s)	1251, 1724
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 658, 688
Bsc4I	CCNNNNNNNGG	1 cut(s)	148
Bse118I	RCCGGY	2 cut(s)	1251, 1724
Bse1I	ACTGG	6 cut(s)	62, 582, 1066, 1120, 1577, 1622
Bse3DI	GCAATG	3 cut(s)	342, 511, 573
BseBI	CCWGG	3 cut(s)	499, 643, 855
BseDI	CCNNGG	7 cut(s)	307, 327, 557, 853, 1030, 1073, 1276
BseGI	GGATG	2 cut(s)	1125, 1195
BseLI	CCNNNNNNNGG	1 cut(s)	148
BseMI	GCAATG	3 cut(s)	342, 511, 573
BseMII	CTCAG	3 cut(s)	599, 708, 1432
BseNI	ACTGG	6 cut(s)	62, 582, 1066, 1120, 1577, 1622
BsePI	GCGCGC	1 cut(s)	347
BseRI	GAGGAG	1 cut(s)	1019
BseSI	GKGCMC	1 cut(s)	17
BseXI	GCAGC	3 cut(s)	100, 1141, 1868
BseYI	CCCAGC	1 cut(s)	761
BsgI	GTGCAG	1 cut(s)	1148
Bsh1236I	CGCG	5 cut(s)	165, 220, 291, 349, 437
BshFI	GGCC	5 cut(s)	5, 359, 386, 556, 1796
BshNI	GGYRCC	1 cut(s)	311
BshTI	ACCGGT	2 cut(s)	1251, 1724
BsiHKAI	GWGCWC	1 cut(s)	17
BsiHKCI	CYCGRG	1 cut(s)	133
BsiSI	CCGG	5 cut(s)	309, 387, 395, 1252, 1725
BslFI	GGGAC	3 cut(s)	221, 289, 461
BslI	CCNNNNNNNGG	1 cut(s)	148
BsmAI	GTCTC	1 cut(s)	1349
BsmFI	GGGAC	3 cut(s)	221, 289, 461
BsmI	GAATGC	2 cut(s)	1200, 1654
BsnI	GGCC	5 cut(s)	5, 359, 386, 556, 1796
BsoBI	CYCGRG	1 cut(s)	133
Bsp119I	TTCGAA	1 cut(s)	417
Bsp1286I	GDGCHC	1 cut(s)	17
Bsp68I	TCGCGA	1 cut(s)	437
BspANI	GGCC	5 cut(s)	5, 359, 386, 556, 1796
BspCNI	CTCAG	3 cut(s)	600, 709, 1431
BspFNI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BspHI	TCATGA	2 cut(s)	1014, 1471
BspLI	GGNNCC	5 cut(s)	153, 313, 392, 1575, 1620
BspMAI	CTGCAG	1 cut(s)	1882
BspMI	ACCTGC	1 cut(s)	616
BspPI	GGATC	1 cut(s)	1159
BspT104I	TTCGAA	1 cut(s)	417
BspT107I	GGYRCC	1 cut(s)	311
BsrDI	GCAATG	3 cut(s)	342, 511, 573
BsrFI	RCCGGY	2 cut(s)	1251, 1724
BsrI	ACTGG	6 cut(s)	62, 582, 1066, 1120, 1577, 1622
BssAI	RCCGGY	2 cut(s)	1251, 1724
BssECI	CCNNGG	7 cut(s)	307, 327, 557, 853, 1030, 1073, 1276
BssHII	GCGCGC	1 cut(s)	347
BssNI	GRCGYC	4 cut(s)	312, 369, 401, 1294
BssSI	CACGAG	1 cut(s)	1686
BssT1I	CCWWGG	2 cut(s)	327, 1276
Bst2BI	CACGAG	1 cut(s)	1686
Bst2UI	CCWGG	3 cut(s)	499, 643, 855
Bst4CI	ACNGT	6 cut(s)	470, 793, 805, 1360, 1519, 1720
BstACI	GRCGYC	4 cut(s)	312, 369, 401, 1294
BstAPI	GCANNNNNTGC	1 cut(s)	1886
BstBI	TTCGAA	1 cut(s)	417
BstC8I	GCNNGC	6 cut(s)	299, 349, 353, 357, 709, 1267
BstDEI	CTNAG	4 cut(s)	608, 717, 1286, 1418
BstDSI	CCRYGG	1 cut(s)	1030
BstEII	GGTNACC	2 cut(s)	1483, 1720
BstF5I	GGATG	2 cut(s)	1125, 1195
BstFNI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BstH2I	RGCGCY	1 cut(s)	315
BstHHI	GCGC	5 cut(s)	167, 222, 314, 349, 351
BstMAI	GTCTC	1 cut(s)	1349
BstMWI	GCNNNNNNNGC	9 cut(s)	361, 533, 654, 675, 992, 1129, 1595, 1865, 1886
BstNI	CCWGG	3 cut(s)	499, 643, 855
BstNSI	RCATGY	3 cut(s)	1043, 1127, 1560
BstPI	GGTNACC	2 cut(s)	1483, 1720
BstSCI	CCNGG	6 cut(s)	307, 386, 393, 497, 641, 853
BstSFI	CTRYAG	3 cut(s)	1478, 1509, 1878
BstSLI	GKGCMC	1 cut(s)	17
BstUI	CGCG	5 cut(s)	165, 220, 291, 349, 437
BstV1I	GCAGC	3 cut(s)	100, 1141, 1868
BstV2I	GAAGAC	1 cut(s)	620
BstX2I	RGATCY	2 cut(s)	713, 1151
BstXI	CCANNNNNNTGG	2 cut(s)	649, 1190
BstYI	RGATCY	2 cut(s)	713, 1151
BsuI	GTATCC	1 cut(s)	1190
BsuRI	GGCC	5 cut(s)	5, 359, 386, 556, 1796
BtgI	CCRYGG	1 cut(s)	1030
BtsCI	GGATG	2 cut(s)	1125, 1195
BtsI	GCAGTG	2 cut(s)	883, 1887
BtsIMutI	CAGTG	7 cut(s)	789, 883, 1113, 1404, 1584, 1756, 1887
BtuMI	TCGCGA	1 cut(s)	437
BveI	ACCTGC	1 cut(s)	616
Cac8I	GCNNGC	6 cut(s)	299, 349, 353, 357, 709, 1267
CaiI	CAGNNNCTG	2 cut(s)	1517, 1886
CciI	TCATGA	2 cut(s)	1014, 1471
CfoI	GCGC	5 cut(s)	167, 222, 314, 349, 351
Cfr10I	RCCGGY	2 cut(s)	1251, 1724
Cfr13I	GGNCC	2 cut(s)	1254, 1574
CseI	GACGC	3 cut(s)	358, 390, 1302
Csp6I	GTAC	5 cut(s)	544, 1043, 1111, 1212, 1639
CspAI	ACCGGT	2 cut(s)	1251, 1724
CviAII	CATG	8 cut(s)	649, 1015, 1040, 1124, 1169, 1298, 1472, 1557
CviQI	GTAC	5 cut(s)	544, 1043, 1111, 1212, 1639
DdeI	CTNAG	4 cut(s)	608, 717, 1286, 1418
DinI	GGCGCC	1 cut(s)	313
DrdI	GACNNNNNNGTC	1 cut(s)	473
DseDI	GACNNNNNNGTC	1 cut(s)	473
EaeI	YGGCCR	2 cut(s)	3, 357
Eco130I	CCWWGG	2 cut(s)	327, 1276
Eco147I	AGGCCT	1 cut(s)	1796
Eco32I	GATATC	1 cut(s)	1734
Eco47I	GGWCC	2 cut(s)	1254, 1574
Eco57I	CTGAAG	2 cut(s)	720, 1254
Eco88I	CYCGRG	1 cut(s)	133
Eco91I	GGTNACC	2 cut(s)	1483, 1720
EcoO65I	GGTNACC	2 cut(s)	1483, 1720
EcoRII	CCWGG	3 cut(s)	497, 641, 853
EcoRV	GATATC	1 cut(s)	1734
EcoT14I	CCWWGG	2 cut(s)	327, 1276
EcoT22I	ATGCAT	1 cut(s)	1125
EgeI	GGCGCC	1 cut(s)	313
EheI	GGCGCC	1 cut(s)	313
ErhI	CCWWGG	2 cut(s)	327, 1276
FaeI	CATG	8 cut(s)	652, 1018, 1043, 1127, 1172, 1301, 1475, 1560
FalI	AAGNNNNNCTT	2 cut(s)	398, 430
FaqI	GGGAC	3 cut(s)	221, 289, 461
FatI	CATG	8 cut(s)	648, 1014, 1039, 1123, 1168, 1297, 1471, 1556
FauI	CCCGC	1 cut(s)	919
FauNDI	CATATG	2 cut(s)	594, 1216
FbaI	TGATCA	2 cut(s)	870, 1387
FblI	GTMKAC	1 cut(s)	372
Fnu4HI	GCNGC	8 cut(s)	6, 39, 89, 168, 223, 399, 1130, 1857
FokI	GGATG	2 cut(s)	1132, 1202
Fsp4HI	GCNGC	8 cut(s)	6, 39, 89, 168, 223, 399, 1130, 1857
FspBI	CTAG	5 cut(s)	663, 945, 1277, 1785, 1823
GlaI	GCGC	5 cut(s)	166, 221, 313, 348, 350
GluI	GCNGC	8 cut(s)	6, 39, 89, 168, 223, 399, 1130, 1857
GsaI	CCCAGC	1 cut(s)	765
GsuI	CTGGAG	1 cut(s)	45
HaeII	RGCGCY	1 cut(s)	315
HaeIII	GGCC	5 cut(s)	5, 359, 386, 556, 1796
HapII	CCGG	5 cut(s)	309, 387, 395, 1252, 1725
HgaI	GACGC	3 cut(s)	358, 390, 1302
HhaI	GCGC	5 cut(s)	167, 222, 314, 349, 351
Hin1I	GRCGYC	4 cut(s)	312, 369, 401, 1294
Hin1II	CATG	8 cut(s)	652, 1018, 1043, 1127, 1172, 1301, 1475, 1560
Hin6I	GCGC	5 cut(s)	165, 220, 312, 347, 349
HinP1I	GCGC	5 cut(s)	165, 220, 312, 347, 349
HincII	GTYRAC	1 cut(s)	373
HindII	GTYRAC	1 cut(s)	373
HinfI	GANTC	7 cut(s)	562, 589, 772, 1065, 1401, 1583, 1862
HpaII	CCGG	5 cut(s)	309, 387, 395, 1252, 1725
HphI	GGTGA	8 cut(s)	116, 313, 640, 664, 758, 989, 1714, 1739
Hpy166II	GTNNAC	4 cut(s)	15, 373, 1113, 1292
Hpy8I	GTNNAC	4 cut(s)	15, 373, 1113, 1292
Hpy99I	CGWCG	3 cut(s)	296, 374, 406
HpyAV	CCTTC	6 cut(s)	400, 505, 613, 715, 1609, 1655
HpyCH4III	ACNGT	6 cut(s)	470, 793, 805, 1360, 1519, 1720
HpyCH4IV	ACGT	3 cut(s)	234, 867, 1210
HpyF10VI	GCNNNNNNNGC	9 cut(s)	361, 533, 654, 675, 992, 1129, 1595, 1865, 1886
HpyF3I	CTNAG	4 cut(s)	608, 717, 1286, 1418
HpySE526I	ACGT	3 cut(s)	234, 867, 1210
Hsp92I	GRCGYC	4 cut(s)	312, 369, 401, 1294
Hsp92II	CATG	8 cut(s)	652, 1018, 1043, 1127, 1172, 1301, 1475, 1560
HspAI	GCGC	5 cut(s)	165, 220, 312, 347, 349
KasI	GGCGCC	1 cut(s)	311
Ksp22I	TGATCA	2 cut(s)	870, 1387
LmnI	GCTCC	8 cut(s)	96, 157, 291, 598, 622, 645, 666, 1036
Lsp1109I	GCAGC	3 cut(s)	100, 1141, 1868
LweI	GCATC	8 cut(s)	59, 255, 694, 1110, 1399, 1522, 1660, 1809
MaeI	CTAG	5 cut(s)	663, 945, 1277, 1785, 1823
MaeII	ACGT	3 cut(s)	234, 867, 1210
MaeIII	GTNAC	7 cut(s)	122, 958, 1402, 1483, 1624, 1654, 1720
MboII	GAAGA	7 cut(s)	42, 45, 48, 439, 625, 713, 1247
MfeI	CAATTG	1 cut(s)	950
MflI	RGATCY	2 cut(s)	713, 1151
MhlI	GDGCHC	1 cut(s)	17
MlsI	TGGCCA	1 cut(s)	359
MluCI	AATT	9 cut(s)	451, 725, 815, 858, 950, 972, 1221, 1527, 1695
MluNI	TGGCCA	1 cut(s)	359
Mly113I	GGCGCC	1 cut(s)	312
MlyI	GAGTC	2 cut(s)	766, 1410
MmeI	TCCRAC	3 cut(s)	276, 1129, 1546
Mox20I	TGGCCA	1 cut(s)	359
Mph1103I	ATGCAT	1 cut(s)	1125
MroXI	GAANNNNTTC	2 cut(s)	1443, 1839
MscI	TGGCCA	1 cut(s)	359
MseI	TTAA	4 cut(s)	519, 840, 1022, 1521
MslI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
Msp20I	TGGCCA	1 cut(s)	359
MspA1I	CMGCKG	3 cut(s)	8, 120, 355
MspI	CCGG	5 cut(s)	309, 387, 395, 1252, 1725
MspR9I	CCNGG	6 cut(s)	309, 388, 395, 499, 643, 855
MunI	CAATTG	1 cut(s)	950
Mva1269I	GAATGC	2 cut(s)	1200, 1654
MvaI	CCWGG	3 cut(s)	499, 643, 855
MvnI	CGCG	5 cut(s)	165, 220, 291, 349, 437
MwoI	GCNNNNNNNGC	9 cut(s)	361, 533, 654, 675, 992, 1129, 1595, 1865, 1886
NarI	GGCGCC	1 cut(s)	312
NciI	CCSGG	3 cut(s)	309, 388, 395
NdeI	CATATG	2 cut(s)	594, 1216
NlaIII	CATG	8 cut(s)	652, 1018, 1043, 1127, 1172, 1301, 1475, 1560
NlaIV	GGNNCC	5 cut(s)	153, 313, 392, 1575, 1620
NmeAIII	GCCGAG	3 cut(s)	345, 582, 1054
NmuCI	GTSAC	4 cut(s)	122, 1402, 1654, 1720
NruI	TCGCGA	1 cut(s)	437
NsiI	ATGCAT	1 cut(s)	1125
NspI	RCATGY	3 cut(s)	1043, 1127, 1560
NspV	TTCGAA	1 cut(s)	417
PagI	TCATGA	2 cut(s)	1014, 1471
PaqCI	CACCTGC	1 cut(s)	616
PauI	GCGCGC	1 cut(s)	347
PceI	AGGCCT	1 cut(s)	1796
PcsI	WCGNNNNNNNCGW	2 cut(s)	378, 453
PctI	GAATGC	2 cut(s)	1200, 1654
PdmI	GAANNNNTTC	2 cut(s)	1443, 1839
PfeI	GAWTC	5 cut(s)	562, 589, 1065, 1583, 1862
PflFI	GACNNNGTC	1 cut(s)	233
PinAI	ACCGGT	2 cut(s)	1251, 1724
PkrI	GCNGC	8 cut(s)	7, 40, 90, 169, 224, 400, 1131, 1858
PleI	GAGTC	2 cut(s)	766, 1409
PluTI	GGCGCC	1 cut(s)	315
PpsI	GAGTC	2 cut(s)	766, 1409
PsiI	TTATAA	1 cut(s)	755
Psp6I	CCWGG	3 cut(s)	497, 641, 853
PspEI	GGTNACC	2 cut(s)	1483, 1720
PspFI	CCCAGC	1 cut(s)	761
PspGI	CCWGG	3 cut(s)	497, 641, 853
PspN4I	GGNNCC	5 cut(s)	153, 313, 392, 1575, 1620
PspPI	GGNCC	2 cut(s)	1254, 1574
PstI	CTGCAG	1 cut(s)	1882
PstNI	CAGNNNCTG	2 cut(s)	1517, 1886
PsuI	RGATCY	2 cut(s)	713, 1151
PsyI	GACNNNGTC	1 cut(s)	233
PteI	GCGCGC	1 cut(s)	347
PvuII	CAGCTG	1 cut(s)	355
RruI	TCGCGA	1 cut(s)	437
RsaI	GTAC	5 cut(s)	545, 1044, 1112, 1213, 1640
RsaNI	GTAC	5 cut(s)	544, 1043, 1111, 1212, 1639
RseI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
SalI	GTCGAC	1 cut(s)	371
SaqAI	TTAA	4 cut(s)	519, 840, 1022, 1521
SatI	GCNGC	8 cut(s)	6, 39, 89, 168, 223, 399, 1130, 1857
Sau96I	GGNCC	2 cut(s)	1254, 1574
ScaI	AGTACT	1 cut(s)	1640
SchI	GAGTC	2 cut(s)	766, 1410
ScrFI	CCNGG	6 cut(s)	309, 388, 395, 499, 643, 855
SduI	GDGCHC	1 cut(s)	17
SfaNI	GCATC	8 cut(s)	59, 255, 694, 1110, 1399, 1522, 1660, 1809
SfcI	CTRYAG	3 cut(s)	1478, 1509, 1878
SfoI	GGCGCC	1 cut(s)	313
SfuI	TTCGAA	1 cut(s)	417
SgrAI	CRCCGGYG	1 cut(s)	1724
SinI	GGWCC	2 cut(s)	1254, 1574
SmiMI	CAYNNNNRTG	3 cut(s)	1118, 1173, 1188
SmlI	CTYRAG	2 cut(s)	1199, 1352
SmoI	CTYRAG	2 cut(s)	1199, 1352
Sse9I	AATT	9 cut(s)	451, 725, 815, 858, 950, 972, 1221, 1527, 1695
SseBI	AGGCCT	1 cut(s)	1796
SspDI	GGCGCC	1 cut(s)	311
SspMI	CTAG	5 cut(s)	663, 945, 1277, 1785, 1823
StuI	AGGCCT	1 cut(s)	1796
StyD4I	CCNGG	6 cut(s)	307, 386, 393, 497, 641, 853
StyI	CCWWGG	2 cut(s)	327, 1276
TaaI	ACNGT	6 cut(s)	470, 793, 805, 1360, 1519, 1720
TaiI	ACGT	3 cut(s)	237, 870, 1213
TaqI	TCGA	9 cut(s)	276, 339, 372, 381, 404, 417, 633, 687, 1699
TasI	AATT	9 cut(s)	451, 725, 815, 858, 950, 972, 1221, 1527, 1695
TatI	WGTACW	3 cut(s)	1042, 1110, 1638
TauI	GCSGC	5 cut(s)	8, 41, 170, 225, 401
TfiI	GAWTC	5 cut(s)	562, 589, 1065, 1583, 1862
Tru1I	TTAA	4 cut(s)	519, 840, 1022, 1521
Tru9I	TTAA	4 cut(s)	519, 840, 1022, 1521
TscAI	CASTG	7 cut(s)	796, 890, 1120, 1411, 1584, 1756, 1887
TseFI	GTSAC	4 cut(s)	122, 1402, 1654, 1720
TseI	GCWGC	3 cut(s)	88, 1129, 1856
Tsp45I	GTSAC	4 cut(s)	122, 1402, 1654, 1720
TspGWI	ACGGA	1 cut(s)	1756
TspRI	CASTG	7 cut(s)	796, 890, 1120, 1411, 1584, 1756, 1887
Tth111I	GACNNNGTC	1 cut(s)	233
VneI	GTGCAC	1 cut(s)	13
VpaK11BI	GGWCC	2 cut(s)	1254, 1574
XapI	RAATTY	3 cut(s)	451, 725, 815
XceI	RCATGY	3 cut(s)	1043, 1127, 1560
XcmI	CCANNNNNNNNNTGG	2 cut(s)	896, 1458
XmaJI	CCTAGG	1 cut(s)	1276
XmiI	GTMKAC	1 cut(s)	372
XmnI	GAANNNNTTC	2 cut(s)	1443, 1839
XspI	CTAG	5 cut(s)	663, 945, 1277, 1785, 1823
ZrmI	AGTACT	1 cut(s)	1640
Zsp2I	ATGCAT	1 cut(s)	1125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.