Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003124

Physical Location & Seq

Genomic Coordinates Forward (+)

62528924 .. 62535950

Gene Structure 7027 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00127070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGAATGTTATTAAGTTGAGACTTGTATTTGTGAAATGGAAGATGTTGAGATTTGAGGAGCATGGTGGCTCCAAGAGAATAAGGATGGATTGTTTAAAACCTCCTCCACTTGTTTCGTACCTCCCTCTTTCTAACAATGGCTGCTGTGTGCACTCCCCCCACACAATCTCGGCCGCCGCCATAGTTTCTTCTTCTCCAGTCCGATGCTCTCACTCCCAATCGCAGCTCCTATTCCGTTCAAATTTCCCGAACCACCGCCGCCACCACAGCCCGAGCCTCCCGATTTCGGCTCCCAATCTCCGCGCCGCTACCAGAGGACGAGTTCTCTGCTCGCAAAACCCTAACCCTGCCCCCACGCGCCGCCCCGACTACGTCCCCAACCGCATCTCCGACCCGAACTATGTCCGCATCTTCGACACCACGCTCCGCGACGGCGAGCAGTCCCCCGGCGCCTCCCTCACCTCCAAGGAGAAGCTCGACATTGCGCGCCAGCTGGCCAAGCTCGGCGTCGACATAATAGAGGCCGGGTGCAATGAGAAGGATATTAAGACGGCGTGGGAGGCGGTCAAGTACGCGAAGAGGCCGAGGATTCATACTTTCATTGCTACCAGTCCGATTCATATGGAGCATAAGCTGAGGAAGACGAAGGAGCAGGTGATCGAAATTGCGAGGAGCATGGTGAGCTATGCTAGAAGCTTGGGTTGCCAAGATGTCGAGTTTAGCCCTGAAGATGCCGGCAGATCTGAGAAGGAATTTCTATATCAGATTTTAGGTGAAGTTATAAAGGCTGGGGCAACAACTCTGAACATTCCTGACACTGTGGGTTATACCGTGCCAACTGAATTTGGTCAGTTGATTGCTGACATTAAATCTAATACCCCTGGAATTGATAACGTGATCGTCTCGACTCACTGCCAAAATGATCTTGGACTTTCTACTGCCAACACTTTAGCGGGGGCAAGTAGAGGTGCTAGGCAATTGGAAGTAACAATCAATGGAATTGGTGAAAGGGCTGGTAATGCTTCATTAGAGGAGGTTGTCATGACCTTAAACTGCCGTGGGGAGCATGTTCTTGGGGGGCTTTATACCGGAATCAATCCTCGGCATATTTATATGACAAGCAAGATGGTTTCAGAGTACACTGGGATGCATGTGCAGCCACACAAGGCTATTGTTGGATCTAATGCTTTTGCACATGAAAGTGGTATCCATCAGGATGGAATGCTCAAGGACAAACGTACATATGAAATTATATCTCCTGAAGATATCGGGTATGACCGGTCCAATGAAGCTGGCATTGTCCTAGGGAAACTTAGTGGACGCCATGCTGTGAAAAAGCAACTTGAACAGCTTGGTTATGAGCTTGATGATGTTCAACTTGAGACTGTATTCTGGCGTTTCAAAGGAGTAGCTGACCAGAAAAAGAGAGTCACTGATGCAGACCTCAGAGCATTGGTGTCAGATGAAGTTTTTCAGCCAGAAGTTGTCTGGAAGCTTCATGATCTACAGGTTACCTGTGGAACTCTTGGTCTTTCTACAGCAACTGTTAAACTAATTGATGCTGATGGGAGAGAGCATATTGCATGTTCAGTTGGAACGGGTCCAGTGGATTCAGCTTACAAGGCTGTTGATCTCATTGTTCAGGAACCAGTAACACTTGTTGAGTACTCTATGAATGCGGTCACAGAAGGAATAGATGCAATAGCCACTACTCGTGTTGCAATTCGCGTTGAAAATAGTGATATGTTCACTGGTGAGATATCTCAACGGACATTCAGTGGGATTGGGGCAGGAATGGACATTGTTGTTTCTAGTGTCAAGGCCTACATTGGTGCATTGAACAAGATGCTAGGTTTCAAGGAAAGGTTTCCATTAGAGGTTGCAGCAGAATCAGCAGAACGAACTGCAGTGTCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003852 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006551 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009098 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0019752 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046912 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00620 ko01100 ko01110 ko01210 ko01230 map00290 map00620 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

HMGL-like LeuA_dimer

Protein Analysis

639

Amino Acids

70.08

Weight (kDa)

7.63

Isoelectric Point (pI)

40.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMGL-like	PF00682	135 - 176	9.7e-12	HMGL-like
HMGL-like	PF00682	177 - 377	1.5e-60	HMGL-like
HCS_D2	PF22617	391 - 475	4.7e-29	Homocitrate synthase post-HMGL domain-like
LeuA_dimer	PF08502	476 - 617	6.5e-32	LeuA allosteric (dimerisation) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18500 AT1G74040 AT1G74040 AT1G74040
fragaria_vesca	FvH4_1g22410 FvH4_1g22460 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570 FvH4_1g22570
malus_domestica	MD01G1019700.v1.1 MD15G1332600.v1.1
prunus_persica	Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1 Prupe.6G172500_v2.0.a1
pyrus_communis	pycom01g04920 pycom15g29280
rosa_chinensis	RchiOBHm_Chr2g0116551 RchiOBHm_Chr2g0116561
rosa_laevigata	RLG00000018287 RLG00000018288
rosa_multiflora	Rmu_sc0001287.1_g000019 Rmu_sc0004191.1_g000022 Rmu_sc0004191.1_g000030
rosa_roxburghii	Rroxscaffold_2G00127060 Rroxscaffold_2G00127070
rosa_rugosa	Rorug02G0207600
rosa_samantha	Rh2AG262500 Rh2AG262600 Rh2BG274100 Rh2BG274400 Rh2DG270700 Rh2DG270800 Rh2DG289200 Rh2DG289300
rosa_wichuraiana	Rw2G020670 Rw2G020680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	782
AarI	CACCTGC	1 cut(s)	643
Acc36I	ACCTGC	1 cut(s)	643
AccB1I	GGYRCC	1 cut(s)	449
AccI	GTMKAC	1 cut(s)	510
AccII	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
AclWI	GGATC	1 cut(s)	1186
AcoI	YGGCCR	2 cut(s)	171, 495
AcsI	RAATTY	3 cut(s)	241, 752, 842
AcuI	CTGAAG	2 cut(s)	747, 1281
AcyI	GRCGYC	3 cut(s)	450, 507, 1321
AfaI	GTAC	5 cut(s)	119, 572, 1139, 1240, 1667
AfiI	CCNNNNNNNGG	1 cut(s)	286
AgeI	ACCGGT	1 cut(s)	1278
AgsI	TTSAA	7 cut(s)	240, 1346, 1376, 1402, 1733, 1840, 1858
AjnI	CCWGG	1 cut(s)	880
Alw21I	GWGCWC	1 cut(s)	153
Alw26I	GTCTC	3 cut(s)	13, 907, 1376
Alw44I	GTGCAC	1 cut(s)	149
AlwI	GGATC	1 cut(s)	1186
AlwNI	CAGNNNCTG	2 cut(s)	1544, 1913
Ama87I	CYCGRG	1 cut(s)	271
AoxI	GGCC	5 cut(s)	171, 495, 522, 581, 1821
ApaLI	GTGCAC	1 cut(s)	149
ApeKI	GCWGC	4 cut(s)	141, 223, 1156, 1883
ApoI	RAATTY	3 cut(s)	241, 752, 842
AsiGI	ACCGGT	1 cut(s)	1278
Asp700I	GAANNNNTTC	2 cut(s)	1470, 1866
AspA2I	CCTAGG	1 cut(s)	1303
AspLEI	GCGC	5 cut(s)	305, 360, 452, 487, 489
AspS9I	GGNCC	2 cut(s)	1281, 1601
AsuC2I	CCSGG	2 cut(s)	447, 526
AsuHPI	GGTGA	6 cut(s)	451, 667, 691, 785, 1016, 1766
AvaI	CYCGRG	1 cut(s)	271
AvaII	GGWCC	2 cut(s)	1281, 1601
AvrII	CCTAGG	1 cut(s)	1303
BaeGI	GKGCMC	1 cut(s)	153
BalI	TGGCCA	1 cut(s)	497
BanI	GGYRCC	1 cut(s)	449
BauI	CACGAG	1 cut(s)	1713
BbsI	GAAGAC	1 cut(s)	647
Bbv12I	GWGCWC	1 cut(s)	153
BbvCI	CCTCAGC	1 cut(s)	635
BbvI	GCAGC	4 cut(s)	128, 235, 1168, 1895
BccI	CCATC	5 cut(s)	79, 1120, 1211, 1218, 1559
BceAI	ACGGC	3 cut(s)	448, 567, 1041
BcgI	CGANNNNNNTGC	2 cut(s)	309, 343
BciT130I	CCWGG	1 cut(s)	882
BciVI	GTATCC	1 cut(s)	1217
BcnI	CCSGG	2 cut(s)	447, 526
BcoDI	GTCTC	3 cut(s)	13, 907, 1376
BfaI	CTAG	5 cut(s)	690, 972, 1304, 1812, 1850
BfmI	CTRYAG	3 cut(s)	1505, 1536, 1905
BfoI	RGCGCY	1 cut(s)	453
BfuAI	ACCTGC	1 cut(s)	643
BfuI	GTATCC	1 cut(s)	1217
BglII	AGATCT	1 cut(s)	740
BisI	GCNGC	9 cut(s)	142, 174, 177, 224, 259, 306, 361, 1157, 1884
BlnI	CCTAGG	1 cut(s)	1303
BlsI	GCNGC	9 cut(s)	143, 175, 178, 225, 260, 307, 362, 1158, 1885
BmcAI	AGTACT	1 cut(s)	1667
Bme1390I	CCNGG	3 cut(s)	447, 526, 882
Bme18I	GGWCC	2 cut(s)	1281, 1601
BmeT110I	CYCGRG	1 cut(s)	271
BmgT120I	GGNCC	2 cut(s)	1281, 1601
BmiI	GGNNCC	5 cut(s)	70, 291, 451, 1602, 1647
BmrFI	CCNGG	3 cut(s)	447, 526, 882
BmrI	ACTGGG	1 cut(s)	1152
BmsI	GCATC	9 cut(s)	194, 393, 417, 721, 1137, 1426, 1549, 1687, 1836
BmuI	ACTGGG	1 cut(s)	1152
BpiI	GAAGAC	1 cut(s)	647
BpmI	CTGGAG	1 cut(s)	180
Bpu10I	CCTNAGC	1 cut(s)	635
BpuEI	CTTGAG	2 cut(s)	1211, 1400
BpuMI	CCSGG	2 cut(s)	447, 526
BsaHI	GRCGYC	3 cut(s)	450, 507, 1321
BsaJI	CCNNGG	7 cut(s)	445, 465, 584, 880, 1057, 1100, 1303
BsaWI	WCCGGW	2 cut(s)	1088, 1278
BsaXI	ACNNNNNCTCC	2 cut(s)	371, 401
Bsc4I	CCNNNNNNNGG	1 cut(s)	286
Bse118I	RCCGGY	2 cut(s)	734, 1278
Bse1I	ACTGG	6 cut(s)	197, 609, 1147, 1604, 1649, 1756
Bse3DI	GCAATG	3 cut(s)	480, 538, 600
BseBI	CCWGG	1 cut(s)	882
BseDI	CCNNGG	7 cut(s)	445, 465, 584, 880, 1057, 1100, 1303
BseGI	GGATG	3 cut(s)	90, 1152, 1222
BseLI	CCNNNNNNNGG	1 cut(s)	286
BseMI	GCAATG	3 cut(s)	480, 538, 600
BseMII	CTCAG	3 cut(s)	626, 735, 1459
BseNI	ACTGG	6 cut(s)	197, 609, 1147, 1604, 1649, 1756
BsePI	GCGCGC	1 cut(s)	485
BseRI	GAGGAG	4 cut(s)	71, 93, 685, 1046
BseSI	GKGCMC	1 cut(s)	153
BseX3I	CGGCCG	1 cut(s)	171
BseXI	GCAGC	4 cut(s)	128, 235, 1168, 1895
BseYI	CCCAGC	1 cut(s)	788
BsgI	GTGCAG	1 cut(s)	1175
Bsh1236I	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
Bsh1285I	CGRYCG	1 cut(s)	174
BshFI	GGCC	5 cut(s)	173, 497, 524, 583, 1823
BshNI	GGYRCC	1 cut(s)	449
BshTI	ACCGGT	1 cut(s)	1278
BsiEI	CGRYCG	1 cut(s)	174
BsiHKAI	GWGCWC	1 cut(s)	153
BsiHKCI	CYCGRG	1 cut(s)	271
BsiSI	CCGG	5 cut(s)	447, 525, 735, 1089, 1279
BslFI	GGGAC	2 cut(s)	359, 427
BslI	CCNNNNNNNGG	1 cut(s)	286
BsmAI	GTCTC	3 cut(s)	13, 907, 1376
BsmBI	CGTCTC	1 cut(s)	907
BsmFI	GGGAC	2 cut(s)	359, 427
BsmI	GAATGC	2 cut(s)	1227, 1681
BsnI	GGCC	5 cut(s)	173, 497, 524, 583, 1823
BsoBI	CYCGRG	1 cut(s)	271
Bsp1286I	GDGCHC	1 cut(s)	153
Bsp143I	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
BspANI	GGCC	5 cut(s)	173, 497, 524, 583, 1823
BspCNI	CTCAG	3 cut(s)	627, 736, 1458
BspFNI	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
BspHI	TCATGA	2 cut(s)	1041, 1498
BspLI	GGNNCC	5 cut(s)	70, 291, 451, 1602, 1647
BspMAI	CTGCAG	1 cut(s)	1909
BspMI	ACCTGC	1 cut(s)	643
BspPI	GGATC	1 cut(s)	1186
BspT107I	GGYRCC	1 cut(s)	449
BsrDI	GCAATG	3 cut(s)	480, 538, 600
BsrFI	RCCGGY	2 cut(s)	734, 1278
BsrI	ACTGG	6 cut(s)	197, 609, 1147, 1604, 1649, 1756
BssAI	RCCGGY	2 cut(s)	734, 1278
BssECI	CCNNGG	7 cut(s)	445, 465, 584, 880, 1057, 1100, 1303
BssHII	GCGCGC	1 cut(s)	485
BssMI	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
BssNI	GRCGYC	3 cut(s)	450, 507, 1321
BssSI	CACGAG	1 cut(s)	1713
BssT1I	CCWWGG	2 cut(s)	465, 1303
Bst2BI	CACGAG	1 cut(s)	1713
Bst2UI	CCWGG	1 cut(s)	882
Bst4CI	ACNGT	4 cut(s)	820, 832, 1387, 1546
Bst6I	CTCTTC	1 cut(s)	572
BstACI	GRCGYC	3 cut(s)	450, 507, 1321
BstAPI	GCANNNNNTGC	1 cut(s)	1913
BstC8I	GCNNGC	7 cut(s)	332, 437, 487, 491, 495, 736, 1294
BstDEI	CTNAG	4 cut(s)	635, 744, 1313, 1445
BstDSI	CCRYGG	1 cut(s)	1057
BstEII	GGTNACC	1 cut(s)	1510
BstF5I	GGATG	3 cut(s)	90, 1152, 1222
BstFNI	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
BstH2I	RGCGCY	1 cut(s)	453
BstHHI	GCGC	5 cut(s)	305, 360, 452, 487, 489
BstKTI	GATC	7 cut(s)	660, 743, 900, 925, 1181, 1504, 1633
BstMAI	GTCTC	3 cut(s)	13, 907, 1376
BstMBI	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
BstMCI	CGRYCG	1 cut(s)	174
BstNI	CCWGG	1 cut(s)	882
BstNSI	RCATGY	3 cut(s)	1070, 1154, 1587
BstPI	GGTNACC	1 cut(s)	1510
BstSCI	CCNGG	3 cut(s)	445, 524, 880
BstSFI	CTRYAG	3 cut(s)	1505, 1536, 1905
BstSLI	GKGCMC	1 cut(s)	153
BstUI	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
BstV1I	GCAGC	4 cut(s)	128, 235, 1168, 1895
BstV2I	GAAGAC	1 cut(s)	647
BstX2I	RGATCY	2 cut(s)	740, 1178
BstXI	CCANNNNNNTGG	1 cut(s)	1217
BstYI	RGATCY	2 cut(s)	740, 1178
BstZI	CGGCCG	1 cut(s)	171
BsuI	GTATCC	1 cut(s)	1217
BsuRI	GGCC	5 cut(s)	173, 497, 524, 583, 1823
BtgI	CCRYGG	1 cut(s)	1057
BtsCI	GGATG	3 cut(s)	90, 1152, 1222
BtsI	GCAGTG	2 cut(s)	910, 1914
BtsIMutI	CAGTG	8 cut(s)	816, 910, 1140, 1431, 1611, 1749, 1783, 1914
BveI	ACCTGC	1 cut(s)	643
Cac8I	GCNNGC	7 cut(s)	332, 437, 487, 491, 495, 736, 1294
CaiI	CAGNNNCTG	2 cut(s)	1544, 1913
CciI	TCATGA	2 cut(s)	1041, 1498
CfoI	GCGC	5 cut(s)	305, 360, 452, 487, 489
Cfr10I	RCCGGY	2 cut(s)	734, 1278
Cfr13I	GGNCC	2 cut(s)	1281, 1601
CseI	GACGC	2 cut(s)	496, 1329
Csp6I	GTAC	5 cut(s)	118, 571, 1138, 1239, 1666
CspAI	ACCGGT	1 cut(s)	1278
CviAII	CATG	9 cut(s)	62, 676, 1042, 1067, 1151, 1196, 1325, 1499, 1584
CviQI	GTAC	5 cut(s)	118, 571, 1138, 1239, 1666
DdeI	CTNAG	4 cut(s)	635, 744, 1313, 1445
DinI	GGCGCC	1 cut(s)	451
DpnI	GATC	7 cut(s)	659, 742, 899, 924, 1180, 1503, 1632
DpnII	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
DraI	TTTAAA	1 cut(s)	96
EaeI	YGGCCR	2 cut(s)	171, 495
EagI	CGGCCG	1 cut(s)	171
Eam1104I	CTCTTC	1 cut(s)	572
EarI	CTCTTC	1 cut(s)	572
EclXI	CGGCCG	1 cut(s)	171
Eco130I	CCWWGG	2 cut(s)	465, 1303
Eco147I	AGGCCT	1 cut(s)	1823
Eco32I	GATATC	2 cut(s)	1267, 1761
Eco47I	GGWCC	2 cut(s)	1281, 1601
Eco52I	CGGCCG	1 cut(s)	171
Eco57I	CTGAAG	2 cut(s)	747, 1281
Eco88I	CYCGRG	1 cut(s)	271
Eco91I	GGTNACC	1 cut(s)	1510
EcoO65I	GGTNACC	1 cut(s)	1510
EcoRII	CCWGG	1 cut(s)	880
EcoRV	GATATC	2 cut(s)	1267, 1761
EcoT14I	CCWWGG	2 cut(s)	465, 1303
EcoT22I	ATGCAT	1 cut(s)	1152
EgeI	GGCGCC	1 cut(s)	451
EheI	GGCGCC	1 cut(s)	451
ErhI	CCWWGG	2 cut(s)	465, 1303
Esp3I	CGTCTC	1 cut(s)	907
FaeI	CATG	9 cut(s)	65, 679, 1045, 1070, 1154, 1199, 1328, 1502, 1587
FaqI	GGGAC	2 cut(s)	359, 427
FatI	CATG	9 cut(s)	61, 675, 1041, 1066, 1150, 1195, 1324, 1498, 1583
FauI	CCCGC	1 cut(s)	946
FauNDI	CATATG	2 cut(s)	621, 1243
FblI	GTMKAC	1 cut(s)	510
Fnu4HI	GCNGC	9 cut(s)	142, 174, 177, 224, 259, 306, 361, 1157, 1884
FokI	GGATG	3 cut(s)	97, 1159, 1229
Fsp4HI	GCNGC	9 cut(s)	142, 174, 177, 224, 259, 306, 361, 1157, 1884
FspBI	CTAG	5 cut(s)	690, 972, 1304, 1812, 1850
GlaI	GCGC	5 cut(s)	304, 359, 451, 486, 488
GluI	GCNGC	9 cut(s)	142, 174, 177, 224, 259, 306, 361, 1157, 1884
GsaI	CCCAGC	1 cut(s)	792
GsuI	CTGGAG	1 cut(s)	180
HaeII	RGCGCY	1 cut(s)	453
HaeIII	GGCC	5 cut(s)	173, 497, 524, 583, 1823
HapII	CCGG	5 cut(s)	447, 525, 735, 1089, 1279
HgaI	GACGC	2 cut(s)	496, 1329
HhaI	GCGC	5 cut(s)	305, 360, 452, 487, 489
Hin1I	GRCGYC	3 cut(s)	450, 507, 1321
Hin1II	CATG	9 cut(s)	65, 679, 1045, 1070, 1154, 1199, 1328, 1502, 1587
Hin6I	GCGC	5 cut(s)	303, 358, 450, 485, 487
HinP1I	GCGC	5 cut(s)	303, 358, 450, 485, 487
HincII	GTYRAC	1 cut(s)	511
HindII	GTYRAC	1 cut(s)	511
HindIII	AAGCTT	2 cut(s)	694, 1493
HinfI	GANTC	7 cut(s)	589, 616, 907, 1092, 1428, 1610, 1889
HpaII	CCGG	5 cut(s)	447, 525, 735, 1089, 1279
HphI	GGTGA	6 cut(s)	451, 667, 691, 785, 1016, 1766
Hpy166II	GTNNAC	5 cut(s)	151, 511, 1140, 1319, 1749
Hpy188I	TCNGA	9 cut(s)	203, 391, 615, 745, 765, 804, 1135, 1448, 1462
Hpy8I	GTNNAC	5 cut(s)	151, 511, 1140, 1319, 1749
Hpy99I	CGWCG	2 cut(s)	434, 512
HpyAV	CCTTC	4 cut(s)	532, 640, 742, 1682
HpyCH4III	ACNGT	4 cut(s)	820, 832, 1387, 1546
HpyCH4IV	ACGT	3 cut(s)	372, 894, 1237
HpyF3I	CTNAG	4 cut(s)	635, 744, 1313, 1445
HpySE526I	ACGT	3 cut(s)	372, 894, 1237
Hsp92I	GRCGYC	3 cut(s)	450, 507, 1321
Hsp92II	CATG	9 cut(s)	65, 679, 1045, 1070, 1154, 1199, 1328, 1502, 1587
HspAI	GCGC	5 cut(s)	303, 358, 450, 485, 487
KasI	GGCGCC	1 cut(s)	449
KroI	GCCGGC	1 cut(s)	734
KroNI	GCCGGC	1 cut(s)	736
Kzo9I	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
LmnI	GCTCC	9 cut(s)	58, 74, 231, 295, 429, 625, 649, 672, 1063
Lsp1109I	GCAGC	4 cut(s)	128, 235, 1168, 1895
LweI	GCATC	9 cut(s)	194, 393, 417, 721, 1137, 1426, 1549, 1687, 1836
MaeI	CTAG	5 cut(s)	690, 972, 1304, 1812, 1850
MaeII	ACGT	3 cut(s)	372, 894, 1237
MaeIII	GTNAC	5 cut(s)	985, 1429, 1510, 1651, 1681
MalI	GATC	7 cut(s)	659, 742, 899, 924, 1180, 1503, 1632
MboI	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
MboII	GAAGA	8 cut(s)	52, 180, 183, 403, 589, 652, 740, 1274
MfeI	CAATTG	1 cut(s)	977
MflI	RGATCY	2 cut(s)	740, 1178
MhlI	GDGCHC	1 cut(s)	153
MlsI	TGGCCA	1 cut(s)	497
MluNI	TGGCCA	1 cut(s)	497
Mly113I	GGCGCC	1 cut(s)	450
MlyI	GAGTC	2 cut(s)	901, 1437
MmeI	TCCRAC	3 cut(s)	414, 1156, 1573
Mox20I	TGGCCA	1 cut(s)	497
Mph1103I	ATGCAT	1 cut(s)	1152
MroNI	GCCGGC	1 cut(s)	734
MroXI	GAANNNNTTC	2 cut(s)	1470, 1866
MscI	TGGCCA	1 cut(s)	497
MseI	TTAA	6 cut(s)	12, 95, 546, 867, 1049, 1548
MslI	CAYNNNNRTG	3 cut(s)	1145, 1200, 1215
Msp20I	TGGCCA	1 cut(s)	497
MspA1I	CMGCKG	1 cut(s)	493
MspI	CCGG	5 cut(s)	447, 525, 735, 1089, 1279
MspR9I	CCNGG	3 cut(s)	447, 526, 882
MunI	CAATTG	1 cut(s)	977
Mva1269I	GAATGC	2 cut(s)	1227, 1681
MvaI	CCWGG	1 cut(s)	882
MvnI	CGCG	6 cut(s)	303, 358, 429, 487, 575, 1728
NaeI	GCCGGC	1 cut(s)	736
NarI	GGCGCC	1 cut(s)	450
NciI	CCSGG	2 cut(s)	447, 526
NdeI	CATATG	2 cut(s)	621, 1243
NdeII	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
NgoMIV	GCCGGC	1 cut(s)	734
NlaIII	CATG	9 cut(s)	65, 679, 1045, 1070, 1154, 1199, 1328, 1502, 1587
NlaIV	GGNNCC	5 cut(s)	70, 291, 451, 1602, 1647
NmeAIII	GCCGAG	4 cut(s)	149, 483, 609, 1081
NmuCI	GTSAC	2 cut(s)	1429, 1681
NsiI	ATGCAT	1 cut(s)	1152
NspI	RCATGY	3 cut(s)	1070, 1154, 1587
PagI	TCATGA	2 cut(s)	1041, 1498
PaqCI	CACCTGC	1 cut(s)	643
PauI	GCGCGC	1 cut(s)	485
PceI	AGGCCT	1 cut(s)	1823
PctI	GAATGC	2 cut(s)	1227, 1681
PdiI	GCCGGC	1 cut(s)	736
PdmI	GAANNNNTTC	2 cut(s)	1470, 1866
PfeI	GAWTC	5 cut(s)	589, 616, 1092, 1610, 1889
PflFI	GACNNNGTC	1 cut(s)	371
PinAI	ACCGGT	1 cut(s)	1278
PkrI	GCNGC	9 cut(s)	143, 175, 178, 225, 260, 307, 362, 1158, 1885
PleI	GAGTC	2 cut(s)	901, 1436
PluTI	GGCGCC	1 cut(s)	453
PpsI	GAGTC	2 cut(s)	901, 1436
PsiI	TTATAA	1 cut(s)	782
Psp6I	CCWGG	1 cut(s)	880
PspEI	GGTNACC	1 cut(s)	1510
PspFI	CCCAGC	1 cut(s)	788
PspGI	CCWGG	1 cut(s)	880
PspN4I	GGNNCC	5 cut(s)	70, 291, 451, 1602, 1647
PspPI	GGNCC	2 cut(s)	1281, 1601
PstI	CTGCAG	1 cut(s)	1909
PstNI	CAGNNNCTG	2 cut(s)	1544, 1913
PsuI	RGATCY	2 cut(s)	740, 1178
PsyI	GACNNNGTC	1 cut(s)	371
PteI	GCGCGC	1 cut(s)	485
PvuII	CAGCTG	1 cut(s)	493
RsaI	GTAC	5 cut(s)	119, 572, 1139, 1240, 1667
RsaNI	GTAC	5 cut(s)	118, 571, 1138, 1239, 1666
RseI	CAYNNNNRTG	3 cut(s)	1145, 1200, 1215
SalI	GTCGAC	1 cut(s)	509
SaqAI	TTAA	6 cut(s)	12, 95, 546, 867, 1049, 1548
SatI	GCNGC	9 cut(s)	142, 174, 177, 224, 259, 306, 361, 1157, 1884
Sau3AI	GATC	7 cut(s)	657, 740, 897, 922, 1178, 1501, 1630
Sau96I	GGNCC	2 cut(s)	1281, 1601
ScaI	AGTACT	1 cut(s)	1667
SchI	GAGTC	2 cut(s)	901, 1437
ScrFI	CCNGG	3 cut(s)	447, 526, 882
SduI	GDGCHC	1 cut(s)	153
SfaNI	GCATC	9 cut(s)	194, 393, 417, 721, 1137, 1426, 1549, 1687, 1836
SfcI	CTRYAG	3 cut(s)	1505, 1536, 1905
SfoI	GGCGCC	1 cut(s)	451
SinI	GGWCC	2 cut(s)	1281, 1601
SmiMI	CAYNNNNRTG	3 cut(s)	1145, 1200, 1215
SmlI	CTYRAG	2 cut(s)	1226, 1379
SmoI	CTYRAG	2 cut(s)	1226, 1379
SseBI	AGGCCT	1 cut(s)	1823
SspDI	GGCGCC	1 cut(s)	449
SspMI	CTAG	5 cut(s)	690, 972, 1304, 1812, 1850
StuI	AGGCCT	1 cut(s)	1823
StyD4I	CCNGG	3 cut(s)	445, 524, 880
StyI	CCWWGG	2 cut(s)	465, 1303
TaaI	ACNGT	4 cut(s)	820, 832, 1387, 1546
TaiI	ACGT	3 cut(s)	375, 897, 1240
TaqI	TCGA	6 cut(s)	414, 477, 510, 660, 714, 905
TatI	WGTACW	2 cut(s)	1137, 1665
TauI	GCSGC	5 cut(s)	176, 179, 261, 308, 363
TfiI	GAWTC	5 cut(s)	589, 616, 1092, 1610, 1889
Tru1I	TTAA	6 cut(s)	12, 95, 546, 867, 1049, 1548
Tru9I	TTAA	6 cut(s)	12, 95, 546, 867, 1049, 1548
TscAI	CASTG	8 cut(s)	823, 917, 1147, 1438, 1611, 1756, 1783, 1914
TseFI	GTSAC	2 cut(s)	1429, 1681
TseI	GCWGC	4 cut(s)	141, 223, 1156, 1883
Tsp45I	GTSAC	2 cut(s)	1429, 1681
TspGWI	ACGGA	2 cut(s)	224, 1783
TspRI	CASTG	8 cut(s)	823, 917, 1147, 1438, 1611, 1756, 1783, 1914
Tth111I	GACNNNGTC	1 cut(s)	371
VneI	GTGCAC	1 cut(s)	149
VpaK11BI	GGWCC	2 cut(s)	1281, 1601
XapI	RAATTY	3 cut(s)	241, 752, 842
XceI	RCATGY	3 cut(s)	1070, 1154, 1587
XcmI	CCANNNNNNNNNTGG	2 cut(s)	923, 1485
XmaJI	CCTAGG	1 cut(s)	1303
XmiI	GTMKAC	1 cut(s)	510
XmnI	GAANNNNTTC	2 cut(s)	1470, 1866
XspI	CTAG	5 cut(s)	690, 972, 1304, 1812, 1850
ZrmI	AGTACT	1 cut(s)	1667
Zsp2I	ATGCAT	1 cut(s)	1152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.