RchiOBHm_Chr3g0451151

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0012473
Physical Location & Seq
Reverse (-)
2361943 .. 2364486
2544 bp
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UTR
Exon/CDS
Intron
PRQ41845

Sequence Viewer

Length: 627 bp
ATGTGCAGAAGCCAGAAGGCCAGGAGTTCCTGTAAACAAATTTTTGTTTGGGCCTTCGGCCCATGTAACCCCAAAAACAAAAGGTTCGACCCGGACCCATTAACCCGGGTCCGAATTCAGGAGGTGCTTTATTTTGGTTTTTGTTCGGGTCTCTCCCTCCCCCTCTTTCTGCACAGATCTGTAATACTGAAGCAGAGTTTCTCTTATCAATTCCAAGTTACTATCAAACTTATAATGGCTGGAAGAGTTGGTATCCCTAATAAGAGCTCGGCACTCATTGCCATGATCGCTGATGAGGACACTGTAACTGGATTTCTGCTGGCTGGAGTGGGCAATGTTGACTTGCGCAGAAAGACAAATTATCTTATAGTTGATTCAAAAACAACAATGAAACAAATTGAGGATGCATTCAAAGAGTTTACTACAAAGGATGACATTGCAATTGTCTTAATCAGTCAATACGTGGCAAACATGATAAGGTTTCTAGTTGATAGCTACAACAAGCCAGTTCCAGCTATATTGGAAATCCCTTCCAAGGATCATCCGTACGACCCTGCGAGTGACTCAGTTCTTTCGCGAGTCAAGTACCTCTTCTCTGCCGAATCCGTGGCAGGAGACAGGCGTTGA

Protein Analysis

208

Amino Acids

23.44

Weight (kDa)

9.37

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_F PF01990 93 - 194 7.3e-29 ATP synthase (F/14-kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012473)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02620
fragaria_vesca FvH4_6g02960
malus_domestica MD04G1224300.v1.1 MD12G1241000.v1.1
prunus_persica Prupe.5G006500_v2.0.a1 Prupe.5G006500_v2.0.a1 Prupe.6G005500_v2.0.a1
pyrus_communis pycom04g19760 pycom12g22070
rosa_chinensis RchiOBHm_Chr3g0451151
rosa_laevigata RLG00000025685
rosa_multiflora Rmu_ssc0000028.1_g000004
rosa_roxburghii Rroxscaffold_6G00428620
rosa_rugosa Rorug02G0632000
rosa_samantha Rh3AG034800 Rh3BG035200 Rh3CG033700 Rh3DG034800
rosa_wichuraiana Rw3G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
Acc16I TGCGCA 1 cut(s) 347
AccII CGCG 1 cut(s) 577
AclWI GGATC 1 cut(s) 546
AcsI RAATTY 2 cut(s) 39, 114
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 2 cut(s) 548, 587
AfiI CCNNNNNNNGG 2 cut(s) 118, 535
AgsI TTSAA 2 cut(s) 378, 412
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 3 cut(s) 267, 495, 515
AluI AGCT 3 cut(s) 267, 495, 515
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 2 cut(s) 155, 609
AlwI GGATC 1 cut(s) 546
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 3 cut(s) 18, 51, 58
ApoI RAATTY 2 cut(s) 39, 114
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 4 cut(s) 51, 59, 94, 109
AsuC2I CCSGG 3 cut(s) 92, 106, 107
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 2 cut(s) 94, 109
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 269
BciT130I CCWGG 1 cut(s) 22
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 3 cut(s) 92, 106, 107
BcoDI GTCTC 2 cut(s) 155, 609
BfaI CTAG 1 cut(s) 485
BfuI GTATCC 1 cut(s) 263
BglII AGATCT 1 cut(s) 176
Bme1390I CCNGG 4 cut(s) 22, 92, 106, 107
Bme18I GGWCC 2 cut(s) 94, 109
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 4 cut(s) 51, 59, 94, 109
BmiI GGNNCC 2 cut(s) 96, 110
BmrFI CCNGG 4 cut(s) 22, 92, 106, 107
BmsI GCATC 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 345
BpuMI CCSGG 3 cut(s) 92, 106, 107
BsaAI YACGTR 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 105, 534, 606
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 535
Bse1I ACTGG 2 cut(s) 313, 506
Bse3DI GCAATG 3 cut(s) 276, 340, 435
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 3 cut(s) 105, 534, 606
BseGI GGATG 3 cut(s) 409, 436, 541
BseLI CCNNNNNNNGG 2 cut(s) 118, 535
BseMI GCAATG 3 cut(s) 276, 340, 435
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 2 cut(s) 313, 506
BsgI GTGCAG 2 cut(s) 25, 155
Bsh1236I CGCG 1 cut(s) 577
BshFI GGCC 3 cut(s) 20, 53, 60
BsiHKAI GWGCWC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 2 cut(s) 92, 106
BsiWI CGTACG 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 118, 535
BsmAI GTCTC 2 cut(s) 155, 609
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 3 cut(s) 20, 53, 60
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 269
Bsp143I GATC 3 cut(s) 176, 285, 538
Bsp68I TCGCGA 1 cut(s) 577
BspANI GGCC 3 cut(s) 20, 53, 60
BspCNI CTCAG 1 cut(s) 578
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 96, 110
BspPI GGATC 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 3 cut(s) 276, 340, 435
BsrI ACTGG 2 cut(s) 313, 506
BssECI CCNNGG 3 cut(s) 105, 534, 606
BssMI GATC 3 cut(s) 176, 285, 538
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 2 cut(s) 238, 596
BstAPI GCANNNNNTGC 1 cut(s) 278
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 565
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 409, 436, 541
BstFNI CGCG 1 cut(s) 577
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 3 cut(s) 179, 288, 541
BstMAI GTCTC 2 cut(s) 155, 609
BstMBI GATC 3 cut(s) 176, 285, 538
BstMWI GCNNNNNNNGC 2 cut(s) 278, 287
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 4 cut(s) 20, 90, 104, 105
BstUI CGCG 1 cut(s) 577
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 3 cut(s) 20, 53, 60
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 3 cut(s) 409, 436, 541
BtsIMutI CAGTG 1 cut(s) 300
BtuMI TCGCGA 1 cut(s) 577
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 348
Cfr13I GGNCC 4 cut(s) 51, 59, 94, 109
Cfr9I CCCGGG 1 cut(s) 105
Csp6I GTAC 2 cut(s) 547, 586
CviAII CATG 3 cut(s) 63, 283, 472
CviQI GTAC 2 cut(s) 547, 586
DdeI CTNAG 1 cut(s) 565
DpnI GATC 3 cut(s) 178, 287, 540
DpnII GATC 3 cut(s) 176, 285, 538
Eam1104I CTCTTC 2 cut(s) 238, 596
EarI CTCTTC 2 cut(s) 238, 596
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 94, 109
Eco53kI GAGCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 66, 286, 475
FaiI YATR 6 cut(s) 64, 233, 284, 368, 473, 518
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FatI CATG 3 cut(s) 62, 282, 471
FokI GGATG 3 cut(s) 416, 443, 528
FriOI GRGCYC 1 cut(s) 269
FspBI CTAG 1 cut(s) 485
FspI TGCGCA 1 cut(s) 347
GlaI GCGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 3 cut(s) 20, 53, 60
HapII CCGG 2 cut(s) 92, 106
HhaI GCGC 1 cut(s) 348
Hin1II CATG 3 cut(s) 66, 286, 475
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 4 cut(s) 374, 563, 579, 602
HpaII CCGG 2 cut(s) 92, 106
Hpy166II GTNNAC 3 cut(s) 35, 340, 420
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 2 cut(s) 119, 576
Hpy8I GTNNAC 3 cut(s) 35, 340, 420
HpyAV CCTTC 3 cut(s) 10, 64, 540
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 4 cut(s) 6, 172, 407, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 287
HpyF3I CTNAG 1 cut(s) 565
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 3 cut(s) 66, 286, 475
HspAI GCGC 1 cut(s) 346
Kzo9I GATC 3 cut(s) 176, 285, 538
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 4 cut(s) 65, 217, 304, 560
MalI GATC 3 cut(s) 178, 287, 540
MboI GATC 3 cut(s) 176, 285, 538
MboII GAAGA 2 cut(s) 255, 583
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 6 cut(s) 39, 114, 209, 358, 396, 441
MlyI GAGTC 2 cut(s) 557, 588
MnlI CCTC 6 cut(s) 115, 167, 173, 289, 394, 599
Mph1103I ATGCAT 1 cut(s) 409
MseI TTAA 2 cut(s) 101, 449
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 2 cut(s) 92, 106
MspR9I CCNGG 4 cut(s) 22, 92, 106, 107
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 1 cut(s) 22
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 2 cut(s) 278, 287
NciI CCSGG 3 cut(s) 92, 106, 107
NdeII GATC 3 cut(s) 176, 285, 538
NlaIII CATG 3 cut(s) 66, 286, 475
NlaIV GGNNCC 2 cut(s) 96, 110
NmeAIII GCCGAG 1 cut(s) 248
NmuCI GTSAC 1 cut(s) 560
NruI TCGCGA 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 347
NsiI ATGCAT 1 cut(s) 409
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 2 cut(s) 374, 602
Pfl23II CGTACG 1 cut(s) 546
PleI GAGTC 2 cut(s) 557, 587
PpsI GAGTC 2 cut(s) 557, 587
Ppu21I YACGTR 1 cut(s) 463
PsiI TTATAA 1 cut(s) 233
Psp124BI GAGCTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 20
PspGI CCWGG 1 cut(s) 20
PspLI CGTACG 1 cut(s) 546
PspN4I GGNNCC 2 cut(s) 96, 110
PspPI GGNCC 4 cut(s) 51, 59, 94, 109
PsuI RGATCY 1 cut(s) 176
RruI TCGCGA 1 cut(s) 577
RsaI GTAC 2 cut(s) 548, 587
RsaNI GTAC 2 cut(s) 547, 586
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 101, 449
Sau3AI GATC 3 cut(s) 176, 285, 538
Sau96I GGNCC 4 cut(s) 51, 59, 94, 109
SchI GAGTC 2 cut(s) 557, 588
ScrFI CCNGG 4 cut(s) 22, 92, 106, 107
SduI GDGCHC 1 cut(s) 269
SetI ASST 8 cut(s) 86, 126, 269, 465, 482, 497, 517, 591
SfaNI GCATC 1 cut(s) 394
SinI GGWCC 2 cut(s) 94, 109
SmaI CCCGGG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 6 cut(s) 39, 114, 209, 358, 396, 441
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 269
StyD4I CCNGG 4 cut(s) 20, 90, 104, 105
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 87
TasI AATT 6 cut(s) 39, 114, 209, 358, 396, 441
TfiI GAWTC 2 cut(s) 374, 602
Tru1I TTAA 2 cut(s) 101, 449
Tru9I TTAA 2 cut(s) 101, 449
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 534, 595
TspMI CCCGGG 1 cut(s) 105
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 94, 109
XapI RAATTY 2 cut(s) 39, 114
XmaI CCCGGG 1 cut(s) 105
XspI CTAG 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.