Rroxscaffold_6G00428620

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012473
Physical Location & Seq
Reverse (-)
49012630 .. 49014905
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428620.1

Sequence Viewer

Length: 393 bp
ATGGCTGGAAGAGCTGGTATCCCTAATAAGAGCTCGGCACTCATTGCCATGATCGCTGATGAGGACACTGTAACTGGATTTCTGCTGGCTGGAGTGGGCAATGTTGACGTGCGCAGAAAGACAAATTATCTTATAGTTGATTCAAAAACAACAATGAAACAAATTGAGGATGCATTCAAAGAGTTTACCACAAAGGATGACATTGCAATTGTCTTAATCAGTCAATACGTGGCAAACATGATAAGGTTTCTAGTTGATAGCTACAACAAGCCAGTTCCAGCTATATTGGAAATCCCTTCCAAGGATCATCCGTACGACCCTGCGAGTGACTCAGTTCTGTCGCGAGTCAAGTACCTCTTCTCTGCCGAATCCGTGGCAGGAGACAGGCGTTGA

Protein Analysis

130

Amino Acids

14.3

Weight (kDa)

5.78

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_F PF01990 15 - 116 1.9e-29 ATP synthase (F/14-kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012473)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02620
fragaria_vesca FvH4_6g02960
malus_domestica MD04G1224300.v1.1 MD12G1241000.v1.1
prunus_persica Prupe.5G006500_v2.0.a1 Prupe.5G006500_v2.0.a1 Prupe.6G005500_v2.0.a1
pyrus_communis pycom04g19760 pycom12g22070
rosa_chinensis RchiOBHm_Chr3g0451151
rosa_laevigata RLG00000025685
rosa_multiflora Rmu_ssc0000028.1_g000004
rosa_roxburghii Rroxscaffold_6G00428620
rosa_rugosa Rorug02G0632000
rosa_samantha Rh3AG034800 Rh3BG035200 Rh3CG033700 Rh3DG034800
rosa_wichuraiana Rw3G002540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 113
AccII CGCG 1 cut(s) 343
AclWI GGATC 1 cut(s) 312
AfaI GTAC 2 cut(s) 314, 353
AfiI CCNNNNNNNGG 1 cut(s) 301
AgsI TTSAA 2 cut(s) 144, 178
AjiI CACGTC 1 cut(s) 109
AluBI AGCT 4 cut(s) 14, 33, 261, 281
AluI AGCT 4 cut(s) 14, 33, 261, 281
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 375
AlwI GGATC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 1 cut(s) 251
BfuI GTATCC 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 109
BmsI GCATC 1 cut(s) 160
BpmI CTGGAG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 300, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 79, 272
Bse3DI GCAATG 3 cut(s) 42, 106, 201
BseDI CCNNGG 2 cut(s) 300, 372
BseGI GGATG 3 cut(s) 175, 202, 307
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 3 cut(s) 42, 106, 201
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 79, 272
Bsh1236I CGCG 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 35
BsiWI CGTACG 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 375
BsmI GAATGC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 51, 304
Bsp68I TCGCGA 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 344
BspFNI CGCG 1 cut(s) 343
BspPI GGATC 1 cut(s) 312
BspQI GCTCTTC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 42, 106, 201
BsrI ACTGG 2 cut(s) 79, 272
BssECI CCNNGG 2 cut(s) 300, 372
BssMI GATC 2 cut(s) 51, 304
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 2 cut(s) 4, 362
BstAPI GCANNNNNTGC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 331
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 3 cut(s) 175, 202, 307
BstFNI CGCG 1 cut(s) 343
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 2 cut(s) 54, 307
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 2 cut(s) 51, 304
BstMWI GCNNNNNNNGC 3 cut(s) 11, 44, 53
BstUI CGCG 1 cut(s) 343
BsuI GTATCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 372
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 3 cut(s) 175, 202, 307
BtsIMutI CAGTG 1 cut(s) 66
BtuMI TCGCGA 1 cut(s) 343
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 313, 352
CviAII CATG 2 cut(s) 49, 238
CviJI RGCY 7 cut(s) 5, 14, 33, 89, 261, 271, 281
CviKI_1 RGCY 7 cut(s) 5, 14, 33, 89, 261, 271, 281
CviQI GTAC 2 cut(s) 313, 352
DdeI CTNAG 1 cut(s) 331
DpnI GATC 2 cut(s) 53, 306
DpnII GATC 2 cut(s) 51, 304
Eam1104I CTCTTC 2 cut(s) 4, 362
EarI CTCTTC 2 cut(s) 4, 362
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 52, 241
FaiI YATR 4 cut(s) 50, 134, 239, 284
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FatI CATG 2 cut(s) 48, 237
FokI GGATG 3 cut(s) 182, 209, 294
FriOI GRGCYC 1 cut(s) 35
FspBI CTAG 1 cut(s) 251
FspI TGCGCA 1 cut(s) 113
GlaI GCGC 1 cut(s) 113
GsuI CTGGAG 1 cut(s) 111
HhaI GCGC 1 cut(s) 114
Hin1II CATG 2 cut(s) 52, 241
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 4 cut(s) 140, 329, 345, 368
Hpy166II GTNNAC 2 cut(s) 106, 186
Hpy188III TCNNGA 1 cut(s) 342
Hpy8I GTNNAC 2 cut(s) 106, 186
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 2 cut(s) 108, 228
HpyCH4V TGCA 2 cut(s) 173, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 44, 53
HpyF3I CTNAG 1 cut(s) 331
HpySE526I ACGT 2 cut(s) 108, 228
Hsp92II CATG 2 cut(s) 52, 241
HspAI GCGC 1 cut(s) 112
Kzo9I GATC 2 cut(s) 51, 304
LguI GCTCTTC 1 cut(s) 4
LpnPI CCDG 8 cut(s) 60, 71, 75, 285, 291, 333, 363, 370
LweI GCATC 1 cut(s) 160
MaeI CTAG 1 cut(s) 251
MaeII ACGT 2 cut(s) 108, 228
MaeIII GTNAC 2 cut(s) 70, 326
MalI GATC 2 cut(s) 53, 306
MboI GATC 2 cut(s) 51, 304
MboII GAAGA 2 cut(s) 21, 349
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 3 cut(s) 124, 162, 207
MlyI GAGTC 2 cut(s) 323, 354
MnlI CCTC 3 cut(s) 55, 160, 365
Mph1103I ATGCAT 1 cut(s) 175
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 47
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 173
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 3 cut(s) 11, 44, 53
NdeII GATC 2 cut(s) 51, 304
NlaIII CATG 2 cut(s) 52, 241
NmeAIII GCCGAG 1 cut(s) 14
NmuCI GTSAC 1 cut(s) 326
NruI TCGCGA 1 cut(s) 343
NsbI TGCGCA 1 cut(s) 113
NsiI ATGCAT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 4
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 140, 368
Pfl23II CGTACG 1 cut(s) 312
PleI GAGTC 2 cut(s) 323, 353
PpsI GAGTC 2 cut(s) 323, 353
Ppu21I YACGTR 1 cut(s) 229
Psp124BI GAGCTC 1 cut(s) 35
PspLI CGTACG 1 cut(s) 312
RruI TCGCGA 1 cut(s) 343
RsaI GTAC 2 cut(s) 314, 353
RsaNI GTAC 2 cut(s) 313, 352
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 215
Sau3AI GATC 2 cut(s) 51, 304
SchI GAGTC 2 cut(s) 323, 354
SduI GDGCHC 1 cut(s) 35
SetI ASST 8 cut(s) 16, 35, 111, 231, 248, 263, 283, 357
SfaNI GCATC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 3 cut(s) 124, 162, 207
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 35
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 2 cut(s) 111, 231
TasI AATT 3 cut(s) 124, 162, 207
TfiI GAWTC 2 cut(s) 140, 368
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 170
TspGWI ACGGA 2 cut(s) 300, 361
TspRI CASTG 1 cut(s) 73
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.