RchiOBHm_Chr3g0454791
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013738
Physical Location & Seq
Reverse (-)
4754645 .. 4757910
3266 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42179

Sequence Viewer

Length: 2856 bp
ATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAAACTCTCTCTTCTTACCAACCTGTCTTTACAAAGCAATAGGTTCCATGGACAAATTCCATCTTCCTTGGGAATACTTAAAGATTTGGCATTTCTGAATTTGAGCTCAAACAAGCTTGAAGGTAGCATTCCAGGTTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACTTGACTTACAACAATCTATCTGGTGTACTTCCTAAAGAACTCGGCTGGGTGAAGAATCTAACATACTTGGCTCTCTCCGTAAACAGTCTCAGCGGAGTGCTTCCAGCCTCACTTTTAAACTTGACGGAATTGACACAACTAGAACTGGCTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTCTGTATCTCCATACAAACTACCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTACGCGAAATCACTCTGATTGAGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAACAACAAGCTTTCTGGGAGTATTCCAGTGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGCAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATTCCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTAACGGCTCTAACTAACTGTTCATATCTGCAAAAACTACATTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTGATAACCACATTACAGGAAGTATACCAGATAGTCTCGGAAACTTAAGCGGCCTGGTAACCTTGTACTTGTCATACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAGATGACTTAGGGATGATGTCTAACGTTGGTGTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCAATTGAGCTTACTCAATGTTCTCTTATCATGCTGCTTGATCTATCCTTCAACAACTTACAAGGCTCTGTTCCTGTACAGATTGACCGTTTTTCCAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAACTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATTTGTACAGGCAATTGACTTGTCTGGCAACCAGTTTTCTGGGTCCATACCTACATTGATGGGAAGCTGCATATCTTTGGCGTATCTAAACATGTCCAACAACATATTTGAGGGGACTATTCCAGAGTCGTTGAAGTTGATCACTCAACTTGAAGTTTTGGACTTGTCACTTAATCGATTAAATGGCAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGGTTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCTGCACTACTTGGTCGTCCGCCATGCGTAGTTCAAAAGAGGGAATATATAATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCAGTTGCATTTGTCTGTGTGCTGGTTGCTGTCTTCATTTATTGTTTCTTCTTCTTCAGAAAACAGGATTCAAAACCAGAACACCGTGAACACGGAATGCTAAGAGTAATGGGATCCCCAAGCCATCGTGGAAGTCGAACTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGAGTTCAATGAAGCTCATCTCCTGGGTAGAGGGACCTTCGGAACAGTCTATAAAGCAATTGTTGATGACGGGAATACTATTATGGCAGTGAAGGTTCTACATGGTGACAGTATTCAGAGTTACAAAAGCTTTAAGAGGGAATGCCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTAAGGATGGTAGGATATACCTGGAACACACAGTTCAAGGCTCTTATTCTTGAATATGTAGGAAATGGTAACTTGGCAGAGCATCTCTATCCTGGTGGACTAGAGGAAGGAGCATGTGAATTAACATTGTGGGAAAGATTGTCTATAGCTATAGATGTTGCCAATGGCTTGGAGTATCTTCAAGAAGGTTGTCCAGTCCAAATTCTACATTGTGATTTAAAACCAGAGAACGTGCTTATTGACAATGATATGGTGGCTCACGTGGCAGATTTTGGGATTGGAAAGCTCATATCAGCTGACAAACCCAATGAATTGCATGTTAGCACAACGCATTTTCTACGAGGATCGATTGGTTACATTCCCCCAGAATATGGGCAAGGAAATGAGGTTTCAACTAAAGGAGATATCTATAGCTTTGGTGTGATGGTGCTCGAGTTGATAACAAGAAAAAGACCAACAAGCAATATGTTTTCAGATGAGGTTGATCTAAGGAACTGGGTACATTCTTCGTACCCAGACCATGTTTTGGATGTCGTTGACAGTGCACTGAAGGAGATAAAAAGTACAGAGGGTGCTTTGCAAGAGCTTGAGAGATGCTGCATTCAAATGCTTGATGTAGGGTTGATGTGCACAGAAGATACTCCACAAGAAAGACCCTCCATGTCTTTTGTTGTGCAGAAACTGACACAGTGTCTCAAAAGCTCCAAGTTCATGTGA

Protein Analysis

951

Amino Acids

104.63

Weight (kDa)

6.46

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 17 - 75 1.9e-07 Leucine rich repeat
LRR_14 PF23598 58 - 168 2.6e-08 Leucine-rich repeat region
LRR_14 PF23598 253 - 390 2.7e-11 Leucine-rich repeat region
LRR_8 PF13855 290 - 348 8.8e-09 Leucine rich repeat
LRR_4 PF12799 314 - 348 3.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 336 - 396 9.7e-07 Leucine rich repeat
Pkinase PF00069 657 - 939 1.1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 660 - 941 8.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1614
Acc36I ACCTGC 1 cut(s) 463
AccB7I CCANNNNNTGG 4 cut(s) 392, 1643, 2510, 2665
AccI GTMKAC 1 cut(s) 910
AccII CGCG 1 cut(s) 486
AciI CCGC 5 cut(s) 300, 738, 936, 1130, 1724
AclI AACGTT 1 cut(s) 1083
AclWI GGATC 4 cut(s) 373, 1896, 1909, 2491
AcsI RAATTY 8 cut(s) 90, 133, 706, 981, 1009, 1097, 1632, 2340
AcuI CTGAAG 3 cut(s) 513, 1828, 2708
AcvI CACGTG 1 cut(s) 2401
AfaI GTAC 9 cut(s) 234, 569, 953, 1092, 1278, 1377, 2640, 2651, 2704
AfiI CCNNNNNNNGG 5 cut(s) 392, 1139, 1643, 2510, 2665
AflII CTTAAG 1 cut(s) 931
AflIII ACRYGT 2 cut(s) 570, 1461
AhdI GACNNNNNGTC 1 cut(s) 2829
AjnI CCWGG 5 cut(s) 166, 939, 1983, 2159, 2230
AjuI GAANNNNNNNTTGG 2 cut(s) 1117, 1149
Alw21I GWGCWC 4 cut(s) 143, 2571, 2686, 2771
Alw26I GTCTC 3 cut(s) 299, 926, 2837
Alw44I GTGCAC 2 cut(s) 2682, 2767
AlwI GGATC 4 cut(s) 373, 1896, 1909, 2491
AlwNI CAGNNNCTG 2 cut(s) 1643, 2821
Ama87I CYCGRG 1 cut(s) 2570
AoxI GGCC 3 cut(s) 937, 1049, 1121
ApaLI GTGCAC 2 cut(s) 2682, 2767
ApeKI GCWGC 4 cut(s) 1234, 1437, 1787, 2736
ApoI RAATTY 8 cut(s) 90, 133, 706, 981, 1009, 1097, 1632, 2340
AspS9I GGNCC 4 cut(s) 1049, 1121, 1413, 1995
AsuHPI GGTGA 6 cut(s) 15, 268, 863, 1924, 1973, 2078
AsuII TTCGAA 3 cut(s) 1007, 1322, 1335
AvaI CYCGRG 1 cut(s) 2570
AvaII GGWCC 2 cut(s) 1413, 1995
BaeGI GKGCMC 2 cut(s) 2686, 2771
BamHI GGATCC 1 cut(s) 1901
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
BbrPI CACGTG 1 cut(s) 2401
BbsI GAAGAC 1 cut(s) 1813
Bbv12I GWGCWC 4 cut(s) 143, 2571, 2686, 2771
BbvI GCAGC 4 cut(s) 1221, 1424, 1799, 2723
BccI CCATC 7 cut(s) 103, 847, 994, 1423, 1920, 2140, 2557
BceAI ACGGC 1 cut(s) 786
BciT130I CCWGG 5 cut(s) 168, 941, 1985, 2161, 2232
BclI TGATCA 2 cut(s) 1509, 1590
BcoDI GTCTC 3 cut(s) 299, 926, 2837
BfaI CTAG 6 cut(s) 347, 410, 692, 1094, 1176, 2240
BfmI CTRYAG 5 cut(s) 456, 675, 2283, 2289, 2548
BfrI CTTAAG 1 cut(s) 931
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 6 cut(s) 739, 937, 1235, 1438, 1788, 2737
BlsI GCNGC 6 cut(s) 740, 938, 1236, 1439, 1789, 2738
Bme1390I CCNGG 5 cut(s) 168, 941, 1985, 2161, 2232
Bme18I GGWCC 2 cut(s) 1413, 1995
BmeRI GACNNNNNGTC 1 cut(s) 2829
BmeT110I CYCGRG 1 cut(s) 2570
BmgT120I GGNCC 4 cut(s) 1049, 1121, 1413, 1995
BmiI GGNNCC 4 cut(s) 80, 1414, 1903, 1996
BmrFI CCNGG 5 cut(s) 168, 941, 1985, 2161, 2232
BmrI ACTGGG 2 cut(s) 1653, 2644
BmsI GCATC 2 cut(s) 2230, 2723
BmuI ACTGGG 2 cut(s) 1653, 2644
BpiI GAAGAC 1 cut(s) 1813
Bpu14I TTCGAA 3 cut(s) 1007, 1322, 1335
BpuEI CTTGAG 3 cut(s) 533, 722, 2747
Bsa29I ATCGAT 3 cut(s) 1546, 1766, 2486
BsaAI YACGTR 1 cut(s) 2401
BsaJI CCNNGG 3 cut(s) 82, 102, 1984
BsaWI WCCGGW 1 cut(s) 2130
Bsc4I CCNNNNNNNGG 5 cut(s) 392, 1139, 1643, 2510, 2665
Bse1I ACTGG 8 cut(s) 357, 376, 602, 832, 1402, 1648, 2333, 2639
BseBI CCWGG 5 cut(s) 168, 941, 1985, 2161, 2232
BseCI ATCGAT 3 cut(s) 1546, 1766, 2486
BseDI CCNNGG 3 cut(s) 82, 102, 1984
BseGI GGATG 3 cut(s) 1077, 2151, 2674
BseLI CCNNNNNNNGG 5 cut(s) 392, 1139, 1643, 2510, 2665
BseMII CTCAG 3 cut(s) 310, 772, 1148
BseNI ACTGG 8 cut(s) 357, 376, 602, 832, 1402, 1648, 2333, 2639
BseSI GKGCMC 2 cut(s) 2686, 2771
BseXI GCAGC 4 cut(s) 1221, 1424, 1799, 2723
BseYI CCCAGC 1 cut(s) 252
BsgI GTGCAG 3 cut(s) 457, 1692, 2834
Bsh1236I CGCG 1 cut(s) 486
BshFI GGCC 3 cut(s) 939, 1051, 1123
BshVI ATCGAT 3 cut(s) 1546, 1766, 2486
BsiHKAI GWGCWC 4 cut(s) 143, 2571, 2686, 2771
BsiHKCI CYCGRG 1 cut(s) 2570
BsiSI CCGG 1 cut(s) 2131
BslFI GGGAC 2 cut(s) 1498, 2008
BslI CCNNNNNNNGG 5 cut(s) 392, 1139, 1643, 2510, 2665
BsmAI GTCTC 3 cut(s) 299, 926, 2837
BsmFI GGGAC 2 cut(s) 1498, 2008
BsmI GAATGC 4 cut(s) 162, 1890, 2108, 2739
BsnI GGCC 3 cut(s) 939, 1051, 1123
BsoBI CYCGRG 1 cut(s) 2570
Bsp119I TTCGAA 3 cut(s) 1007, 1322, 1335
Bsp1286I GDGCHC 4 cut(s) 143, 2571, 2686, 2771
Bsp1407I TGTACA 3 cut(s) 567, 1276, 1375
Bsp143I GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 5 cut(s) 300, 738, 936, 1130, 1724
BspANI GGCC 3 cut(s) 939, 1051, 1123
BspCNI CTCAG 3 cut(s) 309, 771, 1149
BspDI ATCGAT 3 cut(s) 1546, 1766, 2486
BspFNI CGCG 1 cut(s) 486
BspLI GGNNCC 4 cut(s) 80, 1414, 1903, 1996
BspMAI CTGCAG 2 cut(s) 460, 679
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 4 cut(s) 373, 1896, 1909, 2491
BspT104I TTCGAA 3 cut(s) 1007, 1322, 1335
BspTI CTTAAG 1 cut(s) 931
BsrGI TGTACA 3 cut(s) 567, 1276, 1375
BsrI ACTGG 8 cut(s) 357, 376, 602, 832, 1402, 1648, 2333, 2639
BssECI CCNNGG 3 cut(s) 82, 102, 1984
BssMI GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
BssNAI GTATAC 1 cut(s) 911
BssT1I CCWWGG 2 cut(s) 82, 102
Bst1107I GTATAC 1 cut(s) 911
Bst2UI CCWGG 5 cut(s) 168, 941, 1985, 2161, 2232
Bst6I CTCTTC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 931
BstAPI GCANNNNNTGC 1 cut(s) 1793
BstAUI TGTACA 3 cut(s) 567, 1276, 1375
BstBAI YACGTR 1 cut(s) 2401
BstBI TTCGAA 3 cut(s) 1007, 1322, 1335
BstC8I GCNNGC 5 cut(s) 8, 689, 1001, 1350, 1354
BstDEI CTNAG 6 cut(s) 296, 758, 1066, 1157, 1889, 2627
BstDSI CCRYGG 1 cut(s) 82
BstEII GGTNACC 2 cut(s) 851, 943
BstF5I GGATG 3 cut(s) 1077, 2151, 2674
BstFNI CGCG 1 cut(s) 486
BstKTI GATC 7 cut(s) 381, 1243, 1512, 1593, 1904, 2486, 2626
BstMAI GTCTC 3 cut(s) 299, 926, 2837
BstMBI GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
BstMWI GCNNNNNNNGC 3 cut(s) 1129, 1793, 2402
BstNI CCWGG 5 cut(s) 168, 941, 1985, 2161, 2232
BstNSI RCATGY 4 cut(s) 574, 1465, 2256, 2459
BstPI GGTNACC 2 cut(s) 851, 943
BstSCI CCNGG 5 cut(s) 166, 939, 1983, 2159, 2230
BstSFI CTRYAG 5 cut(s) 456, 675, 2283, 2289, 2548
BstSLI GKGCMC 2 cut(s) 2686, 2771
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 4 cut(s) 1221, 1424, 1799, 2723
BstV2I GAAGAC 1 cut(s) 1813
BstX2I RGATCY 2 cut(s) 378, 1901
BstXI CCANNNNNNTGG 3 cut(s) 730, 1409, 2308
BstYI RGATCY 2 cut(s) 378, 1901
BstZ17I GTATAC 1 cut(s) 911
Bsu15I ATCGAT 3 cut(s) 1546, 1766, 2486
BsuRI GGCC 3 cut(s) 939, 1051, 1123
BsuTUI ATCGAT 3 cut(s) 1546, 1766, 2486
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 1077, 2151, 2674
BtsI GCAGTG 2 cut(s) 474, 2055
BtsIMutI CAGTG 8 cut(s) 474, 609, 825, 1641, 2055, 2684, 2686, 2834
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 5 cut(s) 8, 689, 1001, 1350, 1354
CaiI CAGNNNCTG 2 cut(s) 1643, 2821
Cfr13I GGNCC 4 cut(s) 1049, 1121, 1413, 1995
ClaI ATCGAT 3 cut(s) 1546, 1766, 2486
Csp6I GTAC 9 cut(s) 233, 568, 952, 1091, 1277, 1376, 2639, 2650, 2703
CspCI CAANNNNNGTGG 2 cut(s) 715, 750
CviQI GTAC 9 cut(s) 233, 568, 952, 1091, 1277, 1376, 2639, 2650, 2703
DdeI CTNAG 6 cut(s) 296, 758, 1066, 1157, 1889, 2627
DpnI GATC 7 cut(s) 380, 1242, 1511, 1592, 1903, 2485, 2625
DpnII GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
DraI TTTAAA 2 cut(s) 324, 2358
DriI GACNNNNNGTC 1 cut(s) 2829
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 2829
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 2 cut(s) 1119, 1713
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 82, 102
Eco24I GRGCYC 1 cut(s) 143
Eco32I GATATC 3 cut(s) 21, 1163, 2545
Eco47I GGWCC 2 cut(s) 1413, 1995
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 3 cut(s) 513, 1828, 2708
Eco72I CACGTG 1 cut(s) 2401
Eco88I CYCGRG 1 cut(s) 2570
Eco91I GGTNACC 2 cut(s) 851, 943
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 1995
EcoO65I GGTNACC 2 cut(s) 851, 943
EcoRII CCWGG 5 cut(s) 166, 939, 1983, 2159, 2230
EcoRV GATATC 3 cut(s) 21, 1163, 2545
EcoT14I CCWWGG 2 cut(s) 82, 102
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 2 cut(s) 82, 102
FalI AAGNNNNNCTT 2 cut(s) 1912, 1944
FaqI GGGAC 2 cut(s) 1498, 2008
FbaI TGATCA 2 cut(s) 1509, 1590
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 6 cut(s) 739, 937, 1235, 1438, 1788, 2737
FokI GGATG 3 cut(s) 1084, 2158, 2681
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 6 cut(s) 739, 937, 1235, 1438, 1788, 2737
FspBI CTAG 6 cut(s) 347, 410, 692, 1094, 1176, 2240
GluI GCNGC 6 cut(s) 739, 937, 1235, 1438, 1788, 2737
GsaI CCCAGC 1 cut(s) 256
HaeIII GGCC 3 cut(s) 939, 1051, 1123
HapII CCGG 1 cut(s) 2131
HincII GTYRAC 2 cut(s) 361, 2677
HindII GTYRAC 2 cut(s) 361, 2677
HindIII AAGCTT 5 cut(s) 149, 190, 584, 1001, 2089
HinfI GANTC 7 cut(s) 262, 415, 880, 1496, 1597, 1856, 2134
HpaII CCGG 1 cut(s) 2131
HphI GGTGA 6 cut(s) 15, 268, 863, 1924, 1973, 2078
Hpy188I TCNGA 9 cut(s) 132, 498, 532, 926, 1847, 2003, 2079, 2119, 2614
HpyCH4IV ACGT 3 cut(s) 1083, 2370, 2400
HpyF10VI GCNNNNNNNGC 3 cut(s) 1129, 1793, 2402
HpyF3I CTNAG 6 cut(s) 296, 758, 1066, 1157, 1889, 2627
HpySE526I ACGT 3 cut(s) 1083, 2370, 2400
Ksp22I TGATCA 2 cut(s) 1509, 1590
Kzo9I GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
LmnI GCTCC 3 cut(s) 395, 2249, 2846
Lsp1109I GCAGC 4 cut(s) 1221, 1424, 1799, 2723
LweI GCATC 2 cut(s) 2230, 2723
MaeI CTAG 6 cut(s) 347, 410, 692, 1094, 1176, 2240
MaeII ACGT 3 cut(s) 1083, 2370, 2400
MaeIII GTNAC 9 cut(s) 467, 604, 851, 943, 1536, 2066, 2081, 2207, 2492
MalI GATC 7 cut(s) 380, 1242, 1511, 1592, 1903, 2485, 2625
MboI GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
MfeI CAATTG 3 cut(s) 1203, 1383, 2019
MflI RGATCY 2 cut(s) 378, 1901
MhlI GDGCHC 4 cut(s) 143, 2571, 2686, 2771
MlyI GAGTC 1 cut(s) 1505
MmeI TCCRAC 2 cut(s) 747, 1491
MslI CAYNNNNRTG 1 cut(s) 2744
MspA1I CMGCKG 4 cut(s) 300, 628, 1156, 2435
MspCI CTTAAG 1 cut(s) 931
MspI CCGG 1 cut(s) 2131
MspR9I CCNGG 5 cut(s) 168, 941, 1985, 2161, 2232
MunI CAATTG 3 cut(s) 1203, 1383, 2019
Mva1269I GAATGC 4 cut(s) 162, 1890, 2108, 2739
MvaI CCWGG 5 cut(s) 168, 941, 1985, 2161, 2232
MvnI CGCG 1 cut(s) 486
MwoI GCNNNNNNNGC 3 cut(s) 1129, 1793, 2402
NcoI CCATGG 1 cut(s) 82
NdeII GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
NlaIV GGNNCC 4 cut(s) 80, 1414, 1903, 1996
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 4 cut(s) 604, 851, 1536, 2066
NspI RCATGY 4 cut(s) 574, 1465, 2256, 2459
NspV TTCGAA 3 cut(s) 1007, 1322, 1335
PaeR7I CTCGAG 1 cut(s) 2570
PciI ACATGT 2 cut(s) 570, 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 1921
PctI GAATGC 4 cut(s) 162, 1890, 2108, 2739
PfeI GAWTC 6 cut(s) 262, 415, 880, 1597, 1856, 2134
PflMI CCANNNNNTGG 4 cut(s) 392, 1643, 2510, 2665
PkrI GCNGC 6 cut(s) 740, 938, 1236, 1439, 1789, 2738
PleI GAGTC 1 cut(s) 1504
PmaCI CACGTG 1 cut(s) 2401
PmlI CACGTG 1 cut(s) 2401
PpsI GAGTC 1 cut(s) 1504
Ppu21I YACGTR 1 cut(s) 2401
PpuMI RGGWCCY 1 cut(s) 1995
PscI ACATGT 2 cut(s) 570, 1461
PsiI TTATAA 1 cut(s) 1614
Psp124BI GAGCTC 1 cut(s) 143
Psp1406I AACGTT 1 cut(s) 1083
Psp5II RGGWCCY 1 cut(s) 1995
Psp6I CCWGG 5 cut(s) 166, 939, 1983, 2159, 2230
PspCI CACGTG 1 cut(s) 2401
PspEI GGTNACC 2 cut(s) 851, 943
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 5 cut(s) 166, 939, 1983, 2159, 2230
PspN4I GGNNCC 4 cut(s) 80, 1414, 1903, 1996
PspPI GGNCC 4 cut(s) 1049, 1121, 1413, 1995
PspPPI RGGWCCY 1 cut(s) 1995
PspXI VCTCGAGB 1 cut(s) 2570
PstI CTGCAG 2 cut(s) 460, 679
PstNI CAGNNNCTG 2 cut(s) 1643, 2821
PsuI RGATCY 2 cut(s) 378, 1901
PvuII CAGCTG 3 cut(s) 628, 1156, 2435
RsaI GTAC 9 cut(s) 234, 569, 953, 1092, 1278, 1377, 2640, 2651, 2704
RsaNI GTAC 9 cut(s) 233, 568, 952, 1091, 1277, 1376, 2639, 2650, 2703
RseI CAYNNNNRTG 1 cut(s) 2744
SacI GAGCTC 1 cut(s) 143
SatI GCNGC 6 cut(s) 739, 937, 1235, 1438, 1788, 2737
Sau3AI GATC 7 cut(s) 378, 1240, 1509, 1590, 1901, 2483, 2623
Sau96I GGNCC 4 cut(s) 1049, 1121, 1413, 1995
SbfI CCTGCAGG 1 cut(s) 679
SchI GAGTC 1 cut(s) 1505
ScrFI CCNGG 5 cut(s) 168, 941, 1985, 2161, 2232
SdaI CCTGCAGG 1 cut(s) 679
SduI GDGCHC 4 cut(s) 143, 2571, 2686, 2771
SfaNI GCATC 2 cut(s) 2230, 2723
SfcI CTRYAG 5 cut(s) 456, 675, 2283, 2289, 2548
Sfr274I CTCGAG 1 cut(s) 2570
SfuI TTCGAA 3 cut(s) 1007, 1322, 1335
SinI GGWCC 2 cut(s) 1413, 1995
SlaI CTCGAG 1 cut(s) 2570
SmiMI CAYNNNNRTG 1 cut(s) 2744
SmlI CTYRAG 5 cut(s) 548, 701, 931, 2570, 2726
SmoI CTYRAG 5 cut(s) 548, 701, 931, 2570, 2726
Sse8387I CCTGCAGG 1 cut(s) 679
SsiI CCGC 5 cut(s) 300, 738, 936, 1130, 1724
SspMI CTAG 6 cut(s) 347, 410, 692, 1094, 1176, 2240
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 5 cut(s) 166, 939, 1983, 2159, 2230
StyI CCWWGG 2 cut(s) 82, 102
TaiI ACGT 3 cut(s) 1086, 2373, 2403
TatI WGTACW 7 cut(s) 232, 567, 951, 1090, 1276, 1375, 2702
TauI GCSGC 2 cut(s) 741, 939
TfiI GAWTC 6 cut(s) 262, 415, 880, 1597, 1856, 2134
TscAI CASTG 8 cut(s) 481, 609, 832, 1648, 2055, 2686, 2691, 2834
TseFI GTSAC 4 cut(s) 604, 851, 1536, 2066
TseI GCWGC 4 cut(s) 1234, 1437, 1787, 2736
Tsp45I GTSAC 4 cut(s) 604, 851, 1536, 2066
TspDTI ATGAA 7 cut(s) 19, 162, 777, 1813, 1986, 2463, 2839
TspGWI ACGGA 3 cut(s) 274, 347, 1896
TspRI CASTG 8 cut(s) 481, 609, 832, 1648, 2055, 2686, 2691, 2834
Van91I CCANNNNNTGG 4 cut(s) 392, 1643, 2510, 2665
Vha464I CTTAAG 1 cut(s) 931
VneI GTGCAC 2 cut(s) 2682, 2767
VpaK11BI GGWCC 2 cut(s) 1413, 1995
XapI RAATTY 8 cut(s) 90, 133, 706, 981, 1009, 1097, 1632, 2340
XbaI TCTAGA 1 cut(s) 1175
XceI RCATGY 4 cut(s) 574, 1465, 2256, 2459
XhoI CTCGAG 1 cut(s) 2570
XmiI GTMKAC 1 cut(s) 910
XspI CTAG 6 cut(s) 347, 410, 692, 1094, 1176, 2240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.