Rorug03G0002700
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013738
Physical Location & Seq
Reverse (-)
265888 .. 271849
5962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0002700.1

Sequence Viewer

Length: 1344 bp
ATGCTGTGCTTCTGCCCCTGTTTCGACGACGACCACCACCACCGGAGTTCCGACGACGACCTGGACCTGGAGCCGGTCCTGCTCATTTCCGGAATCGGAGGTTCCATTCTAACTTCTAAACGGAAAAAACTGGGCTTCGACACTCGGGTCTGGGTACGGATCCTATTGGCTGACTTAGAATTCAGGAAGAAACTCTGGTCTCTCTATAATCCCAAAACAGGTTATACAGAAACTTTAGACAAAGACAATGAAATTGTTGTCCCAGATGATGATTTTGGCCTCTATGCAATTGATATTCTAGACCCGTCCCCGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATAAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACCACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAACAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAGTATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCTGTTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTGTAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGACGAAACAGTTTCTGCTGAATCAGCAAAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACCGGGAACTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA

Protein Analysis

447

Amino Acids

50.93

Weight (kDa)

5.87

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 69 - 391 5e-24 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 730
AccI GTMKAC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 2 cut(s) 154, 167
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 2 cut(s) 179, 798
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 5 cut(s) 67, 73, 218, 626, 730
AhdI GACNNNNNGTC 1 cut(s) 545
AjnI CCWGG 3 cut(s) 60, 66, 634
AluBI AGCT 5 cut(s) 693, 727, 910, 935, 1214
AluI AGCT 5 cut(s) 693, 727, 910, 935, 1214
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 4 cut(s) 204, 892, 968, 1140
Alw44I GTGCAC 1 cut(s) 630
AlwI GGATC 2 cut(s) 154, 167
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 144
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 3 cut(s) 277, 576, 762
ApaLI GTGCAC 1 cut(s) 630
ApeKI GCWGC 4 cut(s) 790, 968, 1190, 1214
ApoI RAATTY 2 cut(s) 179, 798
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AspS9I GGNCC 3 cut(s) 64, 76, 860
AsuC2I CCSGG 1 cut(s) 1226
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 3 cut(s) 64, 76, 860
BaeGI GKGCMC 1 cut(s) 634
BalI TGGCCA 1 cut(s) 764
BamHI GGATCC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 4 cut(s) 802, 955, 1177, 1201
BccI CCATC 7 cut(s) 751, 821, 914, 957, 1004, 1136, 1175
BciT130I CCWGG 3 cut(s) 62, 68, 636
BcnI CCSGG 1 cut(s) 1226
BcoDI GTCTC 4 cut(s) 204, 892, 968, 1140
BfaI CTAG 2 cut(s) 299, 545
BfmI CTRYAG 3 cut(s) 668, 930, 1191
BglII AGATCT 1 cut(s) 460
BisI GCNGC 4 cut(s) 791, 969, 1191, 1215
BlsI GCNGC 4 cut(s) 792, 970, 1192, 1216
Bme1390I CCNGG 4 cut(s) 62, 68, 636, 1226
Bme18I GGWCC 3 cut(s) 64, 76, 860
BmeRI GACNNNNNGTC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 64, 76, 860
BmiI GGNNCC 4 cut(s) 72, 103, 161, 397
BmrFI CCNGG 4 cut(s) 62, 68, 636, 1226
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 4 cut(s) 618, 629, 651, 1091
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 1101
BpmI CTGGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 1226
BsaBI GATNNNNATC 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 204
BsaWI WCCGGW 2 cut(s) 42, 89
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 73, 218, 626, 730
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 3 cut(s) 135, 667, 852
Bse8I GATNNNNATC 1 cut(s) 608
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 3 cut(s) 62, 68, 636
BseGI GGATG 4 cut(s) 644, 832, 1147, 1310
BseJI GATNNNNATC 1 cut(s) 608
BseLI CCNNNNNNNGG 5 cut(s) 67, 73, 218, 626, 730
BseMII CTCAG 1 cut(s) 1139
BseNI ACTGG 3 cut(s) 135, 667, 852
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 4 cut(s) 802, 955, 1177, 1201
BshFI GGCC 3 cut(s) 279, 578, 764
BsiHKAI GWGCWC 1 cut(s) 634
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 5 cut(s) 43, 74, 90, 794, 1225
BslFI GGGAC 2 cut(s) 245, 292
BslI CCNNNNNNNGG 5 cut(s) 67, 73, 218, 626, 730
BsmAI GTCTC 4 cut(s) 204, 892, 968, 1140
BsmBI CGTCTC 1 cut(s) 1140
BsmFI GGGAC 2 cut(s) 245, 292
BsmI GAATGC 2 cut(s) 526, 817
BsnI GGCC 3 cut(s) 279, 578, 764
Bso31I GGTCTC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 634
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 2 cut(s) 159, 460
BspANI GGCC 3 cut(s) 279, 578, 764
BspCNI CTCAG 1 cut(s) 1138
BspEI TCCGGA 1 cut(s) 89
BspHI TCATGA 2 cut(s) 352, 571
BspLI GGNNCC 4 cut(s) 72, 103, 161, 397
BspMAI CTGCAG 2 cut(s) 672, 1195
BspPI GGATC 2 cut(s) 154, 167
BspTNI GGTCTC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 3 cut(s) 135, 667, 852
BssAI RCCGGY 1 cut(s) 73
BssMI GATC 2 cut(s) 159, 460
BssNAI GTATAC 1 cut(s) 343
Bst1107I GTATAC 1 cut(s) 343
Bst2UI CCWGG 3 cut(s) 62, 68, 636
Bst4CI ACNGT 3 cut(s) 931, 1156, 1249
BstAPI GCANNNNNTGC 2 cut(s) 615, 1214
BstDEI CTNAG 3 cut(s) 175, 822, 1125
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 4 cut(s) 644, 832, 1147, 1310
BstKTI GATC 2 cut(s) 162, 463
BstMAI GTCTC 4 cut(s) 204, 892, 968, 1140
BstMBI GATC 2 cut(s) 159, 460
BstMWI GCNNNNNNNGC 6 cut(s) 79, 491, 615, 690, 1169, 1214
BstNI CCWGG 3 cut(s) 62, 68, 636
BstSCI CCNGG 4 cut(s) 60, 66, 634, 1224
BstSFI CTRYAG 3 cut(s) 668, 930, 1191
BstSLI GKGCMC 1 cut(s) 634
BstV1I GCAGC 4 cut(s) 802, 955, 1177, 1201
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 2 cut(s) 159, 460
BstYI RGATCY 2 cut(s) 159, 460
BstZ17I GTATAC 1 cut(s) 343
BsuRI GGCC 3 cut(s) 279, 578, 764
BtsCI GGATG 4 cut(s) 644, 832, 1147, 1310
CaiI CAGNNNCTG 1 cut(s) 1160
CciI TCATGA 2 cut(s) 352, 571
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 3 cut(s) 64, 76, 860
Csp6I GTAC 1 cut(s) 155
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 3 cut(s) 353, 550, 572
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 3 cut(s) 175, 822, 1125
DpnI GATC 2 cut(s) 161, 462
DpnII GATC 2 cut(s) 159, 460
DrdI GACNNNNNNGTC 1 cut(s) 146
DriI GACNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 762
Eam1105I GACNNNNNGTC 1 cut(s) 545
Eco147I AGGCCT 1 cut(s) 578
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 64, 76, 860
Eco88I CYCGRG 1 cut(s) 144
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 60, 66, 634
EcoT22I ATGCAT 2 cut(s) 326, 644
Esp3I CGTCTC 1 cut(s) 1140
FaeI CATG 3 cut(s) 356, 553, 575
FalI AAGNNNNNCTT 2 cut(s) 862, 894
FaqI GGGAC 2 cut(s) 245, 292
FatI CATG 3 cut(s) 352, 549, 571
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 791, 969, 1191, 1215
FokI GGATG 4 cut(s) 651, 839, 1154, 1297
Fsp4HI GCNGC 4 cut(s) 791, 969, 1191, 1215
FspBI CTAG 2 cut(s) 299, 545
GluI GCNGC 4 cut(s) 791, 969, 1191, 1215
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 3 cut(s) 279, 578, 764
HapII CCGG 5 cut(s) 43, 74, 90, 794, 1225
Hin1II CATG 3 cut(s) 356, 553, 575
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HindIII AAGCTT 1 cut(s) 691
HinfI GANTC 6 cut(s) 93, 650, 851, 976, 1014, 1166
HpaII CCGG 5 cut(s) 43, 74, 90, 794, 1225
Hpy166II GTNNAC 5 cut(s) 343, 539, 632, 714, 1294
Hpy188I TCNGA 5 cut(s) 52, 98, 428, 1105, 1324
Hpy188III TCNNGA 9 cut(s) 90, 184, 299, 353, 572, 770, 960, 1264, 1314
Hpy8I GTNNAC 5 cut(s) 343, 539, 632, 714, 1294
Hpy99I CGWCG 4 cut(s) 29, 32, 56, 59
HpyAV CCTTC 4 cut(s) 385, 674, 1072, 1282
HpyCH4III ACNGT 3 cut(s) 931, 1156, 1249
HpyCH4V TGCA 9 cut(s) 287, 324, 609, 632, 642, 670, 721, 1193, 1217
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 491, 615, 690, 1169, 1214
HpyF3I CTNAG 3 cut(s) 175, 822, 1125
Hsp92II CATG 3 cut(s) 356, 553, 575
Kpn2I TCCGGA 1 cut(s) 89
Kzo9I GATC 2 cut(s) 159, 460
LmnI GCTCC 1 cut(s) 70
Lsp1109I GCAGC 4 cut(s) 802, 955, 1177, 1201
LweI GCATC 4 cut(s) 618, 629, 651, 1091
MaeI CTAG 2 cut(s) 299, 545
MaeIII GTNAC 2 cut(s) 561, 1238
MalI GATC 2 cut(s) 161, 462
MboI GATC 2 cut(s) 159, 460
MboII GAAGA 3 cut(s) 199, 470, 1106
MfeI CAATTG 2 cut(s) 288, 1089
MflI RGATCY 2 cut(s) 159, 460
MhlI GDGCHC 1 cut(s) 634
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 9 cut(s) 179, 252, 288, 441, 473, 519, 778, 798, 1089
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 2 cut(s) 970, 1023
MmeI TCCRAC 2 cut(s) 75, 451
MnlI CCTC 7 cut(s) 92, 290, 331, 568, 804, 1134, 1329
Mox20I TGGCCA 1 cut(s) 764
Mph1103I ATGCAT 2 cut(s) 326, 644
MroI TCCGGA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 764
MseI TTAA 6 cut(s) 452, 465, 659, 777, 945, 1235
MslI CAYNNNNRTG 1 cut(s) 637
Msp20I TGGCCA 1 cut(s) 764
MspA1I CMGCKG 1 cut(s) 727
MspI CCGG 5 cut(s) 43, 74, 90, 794, 1225
MspR9I CCNGG 4 cut(s) 62, 68, 636, 1226
MunI CAATTG 2 cut(s) 288, 1089
Mva1269I GAATGC 2 cut(s) 526, 817
MvaI CCWGG 3 cut(s) 62, 68, 636
MwoI GCNNNNNNNGC 6 cut(s) 79, 491, 615, 690, 1169, 1214
NciI CCSGG 1 cut(s) 1226
NdeII GATC 2 cut(s) 159, 460
NlaIII CATG 3 cut(s) 356, 553, 575
NlaIV GGNNCC 4 cut(s) 72, 103, 161, 397
NmeAIII GCCGAG 1 cut(s) 997
NmuCI GTSAC 2 cut(s) 561, 1238
NsiI ATGCAT 2 cut(s) 326, 644
PagI TCATGA 2 cut(s) 352, 571
PceI AGGCCT 1 cut(s) 578
PctI GAATGC 2 cut(s) 526, 817
PfeI GAWTC 4 cut(s) 93, 650, 851, 1166
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 4 cut(s) 792, 970, 1192, 1216
PleI GAGTC 2 cut(s) 970, 1022
PpsI GAGTC 2 cut(s) 970, 1022
Psp6I CCWGG 3 cut(s) 60, 66, 634
PspGI CCWGG 3 cut(s) 60, 66, 634
PspN4I GGNNCC 4 cut(s) 72, 103, 161, 397
PspPI GGNCC 3 cut(s) 64, 76, 860
PstI CTGCAG 2 cut(s) 672, 1195
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 2 cut(s) 159, 460
PvuII CAGCTG 1 cut(s) 727
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
RseI CAYNNNNRTG 1 cut(s) 637
SaqAI TTAA 6 cut(s) 452, 465, 659, 777, 945, 1235
SatI GCNGC 4 cut(s) 791, 969, 1191, 1215
Sau3AI GATC 2 cut(s) 159, 460
Sau96I GGNCC 3 cut(s) 64, 76, 860
SchI GAGTC 2 cut(s) 970, 1023
ScrFI CCNGG 4 cut(s) 62, 68, 636, 1226
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 4 cut(s) 618, 629, 651, 1091
SfcI CTRYAG 3 cut(s) 668, 930, 1191
SinI GGWCC 3 cut(s) 64, 76, 860
SmiMI CAYNNNNRTG 1 cut(s) 637
Sse9I AATT 9 cut(s) 179, 252, 288, 441, 473, 519, 778, 798, 1089
SseBI AGGCCT 1 cut(s) 578
SspMI CTAG 2 cut(s) 299, 545
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 4 cut(s) 60, 66, 634, 1224
TaaI ACNGT 3 cut(s) 931, 1156, 1249
TaqI TCGA 3 cut(s) 24, 138, 1076
TasI AATT 9 cut(s) 179, 252, 288, 441, 473, 519, 778, 798, 1089
TfiI GAWTC 4 cut(s) 93, 650, 851, 1166
Tru1I TTAA 6 cut(s) 452, 465, 659, 777, 945, 1235
Tru9I TTAA 6 cut(s) 452, 465, 659, 777, 945, 1235
TseFI GTSAC 2 cut(s) 561, 1238
TseI GCWGC 4 cut(s) 790, 968, 1190, 1214
Tsp45I GTSAC 2 cut(s) 561, 1238
TspDTI ATGAA 3 cut(s) 264, 341, 557
TspGWI ACGGA 2 cut(s) 136, 172
Van91I CCANNNNNTGG 1 cut(s) 730
VneI GTGCAC 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 64, 76, 860
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 2 cut(s) 179, 798
XbaI TCTAGA 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 2 cut(s) 299, 545
Zsp2I ATGCAT 2 cut(s) 326, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.