Rw3G004890
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013738
Physical Location & Seq
Reverse (-)
4111216 .. 4114411
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004890.1

Sequence Viewer

Length: 3051 bp
ATGGCATACTGCAGTCTGAACTATTGTATAACAGAAATGGTACCATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAATGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCTTCTGCAACTGGACTGGAATTACATGCCACCAACGTCTCCAAAACCGGGTTATAGGCCTTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAACCTCTCTCTTCTTACCAACCTGTCTTTACAGAGCAATGGGTTCCATGGACAAATTCCATCTTCCTTGGGATTACTTAAAGAGTTGGCATTTCTGAATGTAAGCTCAAACATGCTTGAAGGTAGCATTCCAGGCTCATTACATGGTTGCCAGAGCTTGAAAGTCATAGACTTGAATGACAACAATCTATCTGGTGTTCTCCCTAAAGAACTCGGCTGGATGAAGAATCTAACATACTTGGCTCTCTCCTTAAACAATCTCAGTGGACTGCTTCCAGCCTCACTTTCAAACTTGACGGAGTTGACAAAACTACAACTGGCCGTCAACTGTTTTACTGGAAAGATCCCACCAGAGCTTGGAGCGTTAAGAAAGCTAGAGATTTTGCATCTCCATACAAACTATCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTACGCGAAATCTCGCTGCTTGAGAATATGTTAAATGGAGAAATCCCTTCAGATATGTTTTCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAACAACAAGCTTTCTGGGAGAATTCCAGTGACCCTCACCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGAGAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATACCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTGACTGCTCTAACTAACTGTTCATATCTGCAAAAACTACACTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTAATAACCACATTACAGGAAGTATACCAGATAGAATCGGAAACTTAAGCGGCCTGGTAACCTTGAACTTGTCATACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAGGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAAAAGACTTGGGGATGATGCCTAACCTTGGTTTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCGATTGAGCTTACTCAATGTTCTCTTATGATGCTGCTTGATCTGTCTTTCAACAACTTAAAAGGGTCTGTTCCTGTACAGATTGACCGTTTTTCCAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAACTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATCTGTACTGGCAATTGACTTGTCTGACAACCAGTTTTCTGGGTCCATACCTACATTGATGGGAAGCTGCATATCTTTGGCGTATCTAAACATGTCCAACAACATATTTGAGGGGACTATTCCAGAGTCGTTGAAGCTGATCACTCAACTTGAAGTTTTGGACTTGTCACTTAATCGATTAAATGGCAGCATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGGTTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCTGCACTACTTGGTCGTCCGCCATGCGTAGTTCAAAAGAGAAAATATAAGATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCAGTTGCAGTAGTCTGTGCTCTGGTTGCTGTCTTTGTTTATTGTTTCTTCTTCTTCAGAAAACGGGATTCAAAACCAGAACACCGTGAACACAGAATGCTAAGAGTAATGGGATCACCAAGCCATCATGGAAGTCGAATTTTCACCCAAAGGGAACTTGAAATTGCAACGGGTGAGTTCAATGAAGCTCATCTCCTGGGTAGAGGGACCTTCGGAACAGTCTATAAAGCAATTGTTGATGATGGGGATACTATTATGGCAGTGAAGGTTCTACATGGTGACAGTATTCAGAGTTACAAAAGCTTCAAGAGGGAATGTCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTAAGAATGGTAGGATATACCTGGAACAAACAGTTCAAGGCTCTTATTCTCGAGTATATTGACAATGGTAACTTAGCACAGCATCTTTATCCTGGTGAACTGGAGGAAGGAGCATGTGAATTATCGTTGAGGGATAGAATGTCAATAGCTATAGATGTTGCCAGTGGCTTGGAGTATCTTCAGGAAGGTTGTCCAGTCCAAATTATGCACTGTGATCTAAAACCAGAAAATGTGCTTATTGACAACTATATGGTGGCTCATGTAGCAGATTTCGGGATTGGGAAGCTCAACGCAGCTGAGAAATCCAACGAATCACATGTTAGCACAACACATTTTCTACGTGGATCAATTGGTTACATTCCCCCAGAATATGGACAAGGAATTGAGGTATCAGCAAAAGGAGACATCTATAGCTTTGGTGTCATGGTGCTTGAGATGATAACAAGAAGAAGACCGACAAGCAATATGTTTCCGGATGGGCTTGATCTAAGGAAGTGGGTGGTTTCTTCGTTCCCAGACCATGTTTGGGATGTTGTTGACAGTACATTAAAGGAGATACAAAGCACACATGGCGCTTTGGATGAGCTTGAGAAATGCTGCATTCAAATGCTTGATGTGGGGTTGATGTGCACAGAAGAGAATCCACAAGAAAGACCTGCTATGTCTTTTGTTGTGCAGAAGTTGAAACAGGTTATGAAAAGCTCCAATATCATTTGA

Protein Analysis

1016

Amino Acids

112.39

Weight (kDa)

7.07

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 50 4.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 123 - 233 8.8e-09 Leucine-rich repeat region
LRR_8 PF13855 129 - 186 8.2e-06 Leucine rich repeat
LRR_8 PF13855 200 - 261 7.1e-06 Leucine rich repeat
LRR_14 PF23598 318 - 431 2.5e-10 Leucine-rich repeat region
LRR_8 PF13855 355 - 413 4.4e-08 Leucine rich repeat
Pkinase PF00069 722 - 1002 1.6e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 725 - 1006 4.2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1809
Acc36I ACCTGC 2 cut(s) 658, 2998
Acc65I GGTACC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 3 cut(s) 587, 1838, 2705
AccI GTMKAC 1 cut(s) 1105
AccII CGCG 1 cut(s) 681
AccIII TCCGGA 1 cut(s) 2806
AciI CCGC 4 cut(s) 933, 1131, 1325, 1919
AclWI GGATC 4 cut(s) 77, 568, 2104, 2686
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 8 cut(s) 285, 792, 901, 1176, 1204, 1292, 1827, 2121
AcuI CTGAAG 3 cut(s) 708, 2023, 2498
AfaI GTAC 5 cut(s) 42, 764, 1473, 1572, 2878
AfiI CCNNNNNNNGG 7 cut(s) 169, 587, 1280, 1334, 1838, 2705, 2860
AflII CTTAAG 1 cut(s) 1126
AflIII ACRYGT 3 cut(s) 765, 1656, 2650
AjnI CCWGG 5 cut(s) 361, 1134, 2178, 2354, 2425
AjuI GAANNNNNNNTTGG 2 cut(s) 1312, 1344
Alw21I GWGCWC 2 cut(s) 2005, 2966
Alw26I GTCTC 2 cut(s) 164, 2730
Alw44I GTGCAC 1 cut(s) 2962
AlwI GGATC 4 cut(s) 77, 568, 2104, 2686
AlwNI CAGNNNCTG 1 cut(s) 1838
Ama87I CYCGRG 1 cut(s) 2384
Aor13HI TCCGGA 1 cut(s) 2806
AoxI GGCC 5 cut(s) 178, 549, 1132, 1244, 1316
ApaLI GTGCAC 1 cut(s) 2962
ApeKI GCWGC 7 cut(s) 691, 1429, 1632, 1752, 1982, 2627, 2931
ApoI RAATTY 8 cut(s) 285, 792, 901, 1176, 1204, 1292, 1827, 2121
Asp718I GGTACC 1 cut(s) 40
AspLEI GCGC 1 cut(s) 2909
AspS9I GGNCC 4 cut(s) 1244, 1316, 1608, 2190
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 8 cut(s) 210, 799, 1058, 2091, 2119, 2168, 2273, 2441
AsuII TTCGAA 3 cut(s) 1202, 1517, 1530
AvaI CYCGRG 1 cut(s) 2384
AvaII GGWCC 2 cut(s) 1608, 2190
BaeGI GKGCMC 1 cut(s) 2966
BanI GGYRCC 1 cut(s) 40
BarI GAAGNNNNNNTAC 2 cut(s) 630, 662
BbsI GAAGAC 1 cut(s) 2791
Bbv12I GWGCWC 2 cut(s) 2005, 2966
BbvI GCAGC 7 cut(s) 678, 1416, 1619, 1764, 1994, 2639, 2918
BccI CCATC 8 cut(s) 82, 298, 1042, 1189, 1618, 2115, 2219, 2804
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 5 cut(s) 363, 1136, 2180, 2356, 2427
BciVI GTATCC 1 cut(s) 2224
BclI TGATCA 2 cut(s) 1704, 1785
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 164, 2730
BfaI CTAG 4 cut(s) 605, 887, 1289, 1371
BfmI CTRYAG 5 cut(s) 10, 651, 870, 2484, 2743
BfoI RGCGCY 1 cut(s) 2910
BfrI CTTAAG 1 cut(s) 1126
BfuAI ACCTGC 2 cut(s) 658, 2998
BfuI GTATCC 1 cut(s) 2224
BisI GCNGC 9 cut(s) 692, 934, 1132, 1430, 1633, 1753, 1983, 2628, 2932
BlsI GCNGC 9 cut(s) 693, 935, 1133, 1431, 1634, 1754, 1984, 2629, 2933
Bme1390I CCNGG 6 cut(s) 170, 363, 1136, 2180, 2356, 2427
Bme18I GGWCC 2 cut(s) 1608, 2190
BmeT110I CYCGRG 1 cut(s) 2384
BmgT120I GGNCC 4 cut(s) 1244, 1316, 1608, 2190
BmiI GGNNCC 4 cut(s) 42, 275, 1609, 2191
BmrFI CCNGG 6 cut(s) 170, 363, 1136, 2180, 2356, 2427
BmrI ACTGGG 1 cut(s) 1848
BmsI GCATC 4 cut(s) 625, 1260, 1416, 2425
BmuI ACTGGG 1 cut(s) 1848
BpiI GAAGAC 1 cut(s) 2791
BpmI CTGGAG 1 cut(s) 2456
Bpu14I TTCGAA 3 cut(s) 1202, 1517, 1530
BpuEI CTTGAG 5 cut(s) 716, 728, 917, 2786, 2942
BpuMI CCSGG 1 cut(s) 170
Bsa29I ATCGAT 2 cut(s) 1741, 1961
BsaAI YACGTR 1 cut(s) 2675
BsaJI CCNNGG 4 cut(s) 277, 297, 1279, 2179
BsaWI WCCGGW 2 cut(s) 2325, 2806
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 587, 1280, 1334, 1838, 2705, 2860
Bse3DI GCAATG 1 cut(s) 274
BseAI TCCGGA 1 cut(s) 2806
BseBI CCWGG 5 cut(s) 363, 1136, 2180, 2356, 2427
BseCI ATCGAT 2 cut(s) 1741, 1961
BseDI CCNNGG 4 cut(s) 277, 297, 1279, 2179
BseGI GGATG 5 cut(s) 456, 1272, 2815, 2869, 2920
BseLI CCNNNNNNNGG 7 cut(s) 169, 587, 1280, 1334, 1838, 2705, 2860
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 4 cut(s) 505, 967, 1343, 2622
BseSI GKGCMC 1 cut(s) 2966
BseXI GCAGC 7 cut(s) 678, 1416, 1619, 1764, 1994, 2639, 2918
BsgI GTGCAG 3 cut(s) 652, 1887, 3029
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 5 cut(s) 180, 551, 1134, 1246, 1318
BshNI GGYRCC 1 cut(s) 40
BshVI ATCGAT 2 cut(s) 1741, 1961
BsiHKAI GWGCWC 2 cut(s) 2005, 2966
BsiHKCI CYCGRG 1 cut(s) 2384
BsiSI CCGG 3 cut(s) 169, 2326, 2807
BslFI GGGAC 2 cut(s) 1693, 2203
BslI CCNNNNNNNGG 7 cut(s) 169, 587, 1280, 1334, 1838, 2705, 2860
BsmAI GTCTC 2 cut(s) 164, 2730
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 2 cut(s) 1693, 2203
BsmI GAATGC 4 cut(s) 357, 1755, 2085, 2934
BsnI GGCC 5 cut(s) 180, 551, 1134, 1246, 1318
BsoBI CYCGRG 1 cut(s) 2384
Bsp119I TTCGAA 3 cut(s) 1202, 1517, 1530
Bsp1286I GDGCHC 2 cut(s) 2005, 2966
Bsp13I TCCGGA 1 cut(s) 2806
Bsp1407I TGTACA 2 cut(s) 762, 1471
Bsp143I GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 4 cut(s) 933, 1131, 1325, 1919
BspANI GGCC 5 cut(s) 180, 551, 1134, 1246, 1318
BspCNI CTCAG 4 cut(s) 504, 966, 1344, 2623
BspDI ATCGAT 2 cut(s) 1741, 1961
BspEI TCCGGA 1 cut(s) 2806
BspFNI CGCG 1 cut(s) 681
BspLI GGNNCC 4 cut(s) 42, 275, 1609, 2191
BspMAI CTGCAG 3 cut(s) 14, 655, 874
BspMI ACCTGC 2 cut(s) 658, 2998
BspPI GGATC 4 cut(s) 77, 568, 2104, 2686
BspT104I TTCGAA 3 cut(s) 1202, 1517, 1530
BspT107I GGYRCC 1 cut(s) 40
BspTI CTTAAG 1 cut(s) 1126
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 2 cut(s) 762, 1471
BssECI CCNNGG 4 cut(s) 277, 297, 1279, 2179
BssMI GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
BssNAI GTATAC 1 cut(s) 1106
BssT1I CCWWGG 3 cut(s) 277, 297, 1279
Bst1107I GTATAC 1 cut(s) 1106
Bst2UI CCWGG 5 cut(s) 363, 1136, 2180, 2356, 2427
Bst6I CTCTTC 2 cut(s) 246, 2964
BstAFI CTTAAG 1 cut(s) 1126
BstAPI GCANNNNNTGC 1 cut(s) 1988
BstAUI TGTACA 2 cut(s) 762, 1471
BstBAI YACGTR 1 cut(s) 2675
BstBI TTCGAA 3 cut(s) 1202, 1517, 1530
BstC8I GCNNGC 5 cut(s) 203, 884, 1196, 1545, 1549
BstDEI CTNAG 7 cut(s) 491, 953, 1352, 2084, 2407, 2631, 2822
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 2 cut(s) 1046, 1138
BstF5I GGATG 5 cut(s) 456, 1272, 2815, 2869, 2920
BstFNI CGCG 1 cut(s) 681
BstH2I RGCGCY 1 cut(s) 2910
BstHHI GCGC 1 cut(s) 2909
BstKTI GATC 9 cut(s) 85, 576, 1438, 1707, 1788, 2099, 2551, 2681, 2821
BstMAI GTCTC 2 cut(s) 164, 2730
BstMBI GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
BstMWI GCNNNNNNNGC 6 cut(s) 363, 1324, 1988, 2009, 2597, 2904
BstNI CCWGG 5 cut(s) 363, 1136, 2180, 2356, 2427
BstNSI RCATGY 6 cut(s) 150, 346, 769, 1660, 2451, 2654
BstPI GGTNACC 2 cut(s) 1046, 1138
BstSCI CCNGG 6 cut(s) 168, 361, 1134, 2178, 2354, 2425
BstSFI CTRYAG 5 cut(s) 10, 651, 870, 2484, 2743
BstSLI GKGCMC 1 cut(s) 2966
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 7 cut(s) 678, 1416, 1619, 1764, 1994, 2639, 2918
BstV2I GAAGAC 1 cut(s) 2791
BstX2I RGATCY 1 cut(s) 573
BstXI CCANNNNNNTGG 3 cut(s) 925, 1604, 2503
BstYI RGATCY 1 cut(s) 573
BstZ17I GTATAC 1 cut(s) 1106
Bsu15I ATCGAT 2 cut(s) 1741, 1961
BsuI GTATCC 1 cut(s) 2224
BsuRI GGCC 5 cut(s) 180, 551, 1134, 1246, 1318
BsuTUI ATCGAT 2 cut(s) 1741, 1961
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 5 cut(s) 456, 1272, 2815, 2869, 2920
BtsI GCAGTG 2 cut(s) 669, 2250
BtsIMutI CAGTG 8 cut(s) 499, 669, 804, 1020, 1836, 2250, 2503, 2542
BveI ACCTGC 2 cut(s) 658, 2998
Cac8I GCNNGC 5 cut(s) 203, 884, 1196, 1545, 1549
CaiI CAGNNNCTG 1 cut(s) 1838
CfoI GCGC 1 cut(s) 2909
Cfr13I GGNCC 4 cut(s) 1244, 1316, 1608, 2190
ClaI ATCGAT 2 cut(s) 1741, 1961
Csp6I GTAC 5 cut(s) 41, 763, 1472, 1571, 2877
CspCI CAANNNNNGTGG 4 cut(s) 475, 510, 910, 945
CviQI GTAC 5 cut(s) 41, 763, 1472, 1571, 2877
DdeI CTNAG 7 cut(s) 491, 953, 1352, 2084, 2407, 2631, 2822
DpnI GATC 9 cut(s) 84, 575, 1437, 1706, 1787, 2098, 2550, 2680, 2820
DpnII GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 2 cut(s) 246, 2964
EarI CTCTTC 2 cut(s) 246, 2964
EciI GGCGGA 2 cut(s) 1314, 1908
Eco130I CCWWGG 3 cut(s) 277, 297, 1279
Eco147I AGGCCT 1 cut(s) 180
Eco32I GATATC 2 cut(s) 216, 1358
Eco47I GGWCC 2 cut(s) 1608, 2190
Eco57I CTGAAG 3 cut(s) 708, 2023, 2498
Eco88I CYCGRG 1 cut(s) 2384
Eco91I GGTNACC 2 cut(s) 1046, 1138
EcoO109I RGGNCCY 1 cut(s) 2190
EcoO65I GGTNACC 2 cut(s) 1046, 1138
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 5 cut(s) 361, 1134, 2178, 2354, 2425
EcoRV GATATC 2 cut(s) 216, 1358
EcoT14I CCWWGG 3 cut(s) 277, 297, 1279
ErhI CCWWGG 3 cut(s) 277, 297, 1279
Esp3I CGTCTC 1 cut(s) 164
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 2 cut(s) 1693, 2203
FbaI TGATCA 2 cut(s) 1704, 1785
FblI GTMKAC 1 cut(s) 1105
Fnu4HI GCNGC 9 cut(s) 692, 934, 1132, 1430, 1633, 1753, 1983, 2628, 2932
FokI GGATG 5 cut(s) 463, 1279, 2822, 2876, 2927
Fsp4HI GCNGC 9 cut(s) 692, 934, 1132, 1430, 1633, 1753, 1983, 2628, 2932
FspBI CTAG 4 cut(s) 605, 887, 1289, 1371
GlaI GCGC 1 cut(s) 2908
GluI GCNGC 9 cut(s) 692, 934, 1132, 1430, 1633, 1753, 1983, 2628, 2932
GsuI CTGGAG 1 cut(s) 2456
HaeII RGCGCY 1 cut(s) 2910
HaeIII GGCC 5 cut(s) 180, 551, 1134, 1246, 1318
HapII CCGG 3 cut(s) 169, 2326, 2807
HhaI GCGC 1 cut(s) 2909
Hin6I GCGC 1 cut(s) 2907
HinP1I GCGC 1 cut(s) 2907
HincII GTYRAC 3 cut(s) 534, 556, 2872
HindII GTYRAC 3 cut(s) 534, 556, 2872
HindIII AAGCTT 2 cut(s) 779, 2284
HinfI GANTC 9 cut(s) 457, 1075, 1116, 1691, 1792, 2051, 2329, 2645, 2974
HpaII CCGG 3 cut(s) 169, 2326, 2807
HphI GGTGA 8 cut(s) 210, 799, 1058, 2091, 2119, 2168, 2273, 2441
HpyCH4IV ACGT 2 cut(s) 157, 2674
HpyF10VI GCNNNNNNNGC 6 cut(s) 363, 1324, 1988, 2009, 2597, 2904
HpyF3I CTNAG 7 cut(s) 491, 953, 1352, 2084, 2407, 2631, 2822
HpySE526I ACGT 2 cut(s) 157, 2674
HspAI GCGC 1 cut(s) 2907
Kpn2I TCCGGA 1 cut(s) 2806
KpnI GGTACC 1 cut(s) 44
Ksp22I TGATCA 2 cut(s) 1704, 1785
Kzo9I GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
LmnI GCTCC 3 cut(s) 590, 2444, 3041
Lsp1109I GCAGC 7 cut(s) 678, 1416, 1619, 1764, 1994, 2639, 2918
LweI GCATC 4 cut(s) 625, 1260, 1416, 2425
MaeI CTAG 4 cut(s) 605, 887, 1289, 1371
MaeII ACGT 2 cut(s) 157, 2674
MaeIII GTNAC 9 cut(s) 662, 799, 1046, 1138, 1731, 2261, 2276, 2402, 2687
MalI GATC 9 cut(s) 84, 575, 1437, 1706, 1787, 2098, 2550, 2680, 2820
MboI GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
MfeI CAATTG 3 cut(s) 1578, 2214, 2682
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 2 cut(s) 2005, 2966
MlyI GAGTC 1 cut(s) 1700
MmeI TCCRAC 3 cut(s) 255, 1686, 2664
MroI TCCGGA 1 cut(s) 2806
MslI CAYNNNNRTG 1 cut(s) 2939
MspA1I CMGCKG 3 cut(s) 823, 1351, 2630
MspCI CTTAAG 1 cut(s) 1126
MspI CCGG 3 cut(s) 169, 2326, 2807
MspR9I CCNGG 6 cut(s) 170, 363, 1136, 2180, 2356, 2427
MunI CAATTG 3 cut(s) 1578, 2214, 2682
Mva1269I GAATGC 4 cut(s) 357, 1755, 2085, 2934
MvaI CCWGG 5 cut(s) 363, 1136, 2180, 2356, 2427
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 6 cut(s) 363, 1324, 1988, 2009, 2597, 2904
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 277
NdeII GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
NlaIV GGNNCC 4 cut(s) 42, 275, 1609, 2191
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 4 cut(s) 799, 1046, 1731, 2261
NspI RCATGY 6 cut(s) 150, 346, 769, 1660, 2451, 2654
NspV TTCGAA 3 cut(s) 1202, 1517, 1530
PaeR7I CTCGAG 1 cut(s) 2384
PceI AGGCCT 1 cut(s) 180
PciI ACATGT 3 cut(s) 765, 1656, 2650
PctI GAATGC 4 cut(s) 357, 1755, 2085, 2934
PfeI GAWTC 8 cut(s) 457, 1075, 1116, 1792, 2051, 2329, 2645, 2974
PflMI CCANNNNNTGG 3 cut(s) 587, 1838, 2705
PkrI GCNGC 9 cut(s) 693, 935, 1133, 1431, 1634, 1754, 1984, 2629, 2933
PleI GAGTC 1 cut(s) 1699
PpsI GAGTC 1 cut(s) 1699
Ppu21I YACGTR 1 cut(s) 2675
PpuMI RGGWCCY 1 cut(s) 2190
PscI ACATGT 3 cut(s) 765, 1656, 2650
PsiI TTATAA 1 cut(s) 1809
Psp5II RGGWCCY 1 cut(s) 2190
Psp6I CCWGG 5 cut(s) 361, 1134, 2178, 2354, 2425
PspEI GGTNACC 2 cut(s) 1046, 1138
PspGI CCWGG 5 cut(s) 361, 1134, 2178, 2354, 2425
PspN4I GGNNCC 4 cut(s) 42, 275, 1609, 2191
PspPI GGNCC 4 cut(s) 1244, 1316, 1608, 2190
PspPPI RGGWCCY 1 cut(s) 2190
PsrI GAACNNNNNNTAC 2 cut(s) 1139, 1171
PstI CTGCAG 3 cut(s) 14, 655, 874
PstNI CAGNNNCTG 1 cut(s) 1838
PsuI RGATCY 1 cut(s) 573
PvuII CAGCTG 3 cut(s) 823, 1351, 2630
RsaI GTAC 5 cut(s) 42, 764, 1473, 1572, 2878
RsaNI GTAC 5 cut(s) 41, 763, 1472, 1571, 2877
RseI CAYNNNNRTG 1 cut(s) 2939
SatI GCNGC 9 cut(s) 692, 934, 1132, 1430, 1633, 1753, 1983, 2628, 2932
Sau3AI GATC 9 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2548, 2678, 2818
Sau96I GGNCC 4 cut(s) 1244, 1316, 1608, 2190
SbfI CCTGCAGG 1 cut(s) 874
SchI GAGTC 1 cut(s) 1700
ScrFI CCNGG 6 cut(s) 170, 363, 1136, 2180, 2356, 2427
SdaI CCTGCAGG 1 cut(s) 874
SduI GDGCHC 2 cut(s) 2005, 2966
SfaNI GCATC 4 cut(s) 625, 1260, 1416, 2425
SfcI CTRYAG 5 cut(s) 10, 651, 870, 2484, 2743
Sfr274I CTCGAG 1 cut(s) 2384
SfuI TTCGAA 3 cut(s) 1202, 1517, 1530
SinI GGWCC 2 cut(s) 1608, 2190
SlaI CTCGAG 1 cut(s) 2384
SmiMI CAYNNNNRTG 1 cut(s) 2939
SmlI CTYRAG 7 cut(s) 695, 743, 896, 1126, 2384, 2765, 2921
SmoI CTYRAG 7 cut(s) 695, 743, 896, 1126, 2384, 2765, 2921
Sse8387I CCTGCAGG 1 cut(s) 874
SseBI AGGCCT 1 cut(s) 180
SsiI CCGC 4 cut(s) 933, 1131, 1325, 1919
SspMI CTAG 4 cut(s) 605, 887, 1289, 1371
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 6 cut(s) 168, 361, 1134, 2178, 2354, 2425
StyI CCWWGG 3 cut(s) 277, 297, 1279
TaiI ACGT 2 cut(s) 160, 2677
TaqI TCGA 9 cut(s) 636, 1202, 1307, 1517, 1530, 1741, 1961, 2119, 2385
TaqII GACCGA 1 cut(s) 2803
TatI WGTACW 4 cut(s) 762, 1471, 1570, 2876
TauI GCSGC 2 cut(s) 936, 1134
TfiI GAWTC 8 cut(s) 457, 1075, 1116, 1792, 2051, 2329, 2645, 2974
TscAI CASTG 8 cut(s) 499, 676, 804, 1027, 1843, 2250, 2503, 2549
TseFI GTSAC 4 cut(s) 799, 1046, 1731, 2261
TseI GCWGC 7 cut(s) 691, 1429, 1632, 1752, 1982, 2627, 2931
Tsp45I GTSAC 4 cut(s) 799, 1046, 1731, 2261
TspDTI ATGAA 6 cut(s) 46, 214, 467, 972, 2181, 3044
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 8 cut(s) 499, 676, 804, 1027, 1843, 2250, 2503, 2549
Van91I CCANNNNNTGG 3 cut(s) 587, 1838, 2705
Vha464I CTTAAG 1 cut(s) 1126
VneI GTGCAC 1 cut(s) 2962
VpaK11BI GGWCC 2 cut(s) 1608, 2190
XapI RAATTY 8 cut(s) 285, 792, 901, 1176, 1204, 1292, 1827, 2121
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 6 cut(s) 150, 346, 769, 1660, 2451, 2654
XcmI CCANNNNNNNNNTGG 1 cut(s) 2856
XhoI CTCGAG 1 cut(s) 2384
XmiI GTMKAC 1 cut(s) 1105
XspI CTAG 4 cut(s) 605, 887, 1289, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.