RLG00000025405
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013738
Physical Location & Seq
Forward (+)
45598215 .. 45601412
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025405

Sequence Viewer

Length: 3123 bp
ATGGCATACAATCTGAACTATTGTATTACAGAAATGATACTATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAAGTCTGCATTTCTTTGTGAGGATTCTAGTGATTGCAACGCTCTGCTCAAATTCAAGGAGGAGCTAACAAGTGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCCTCTGCAACTGGACTGGAATTACATGCCACCAACGTCTCCAAAACCGGGTTATAGGCCTTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAACCTCTCTCTTCTTACCAACCTGTCTTTACAGAGCAATGGGTTCCACGGACAAATTCCATCTTCCTTGGGAATACTTAAAGAGTTGGCATTTCTGAATGTGAGCTCAAACAAGCTTGAAGGAAGCATTCCAGTTTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACATGAATAACAACAGTCTATCTGGTGTTCTTCCTAAAGAACTCGGCTTGATGAAGAATCTAACTTACTTGGGTCTCTCCGTAAACAATCTCAGCGGACTGCTTCCAGCCTCGCTTTCAAACTTAACAGAATTGACACAACTACTACTGGGTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTCTGTATCTCCATACAAACTACCTCGAAGGCTCAATACCTGCAGTAATTAGTAACTGCACTGCTTTACGCGAATTCACTCTGATTGAGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAAAAACAAGCTTTCTGGGAGAATTCCAGTGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGGAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATTCCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTGACTGCTCTGACTAACTGTTCATATCTGCAAAAACTACAGTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTAATAACCACATTACAGGAAGTATACCAGATAGTATCGGAAAATTAAGCGGCCTGGTAACCTTGTACTTGTCATACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAAAAGACTTGGGGTTGATGCCTAACCTTGGTTTACTAGAACTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATACCTTTACCTGTCTAGAAATCGCCTAACAGGAAAGTTTCCGATTGAGCTTACTCACTGCTCTCTTATGATGCTGCTTGATCTATCTTTCAACAACTTACAAGGTTCTGTTCCTGTACAGATTGACCATTTTTCCAATTTAGCTCTGTCTCTTAATCTTTCGAACAATAACTTCGAAGGACAGTTGCCTGCAAGCATAGGAAAGCTGGTATCTGTACTGGCAATTGACTTGTCTGACAACCAGTTTTCTGGCTCCATACCTACATTGATCGGAAGCTGCATATCTTTGGCGTATCTAAACATGTCCAACAACATATTTGAGGGGACTATTCCAGAGTCATTGAAGTTGATCACTCAACTTGAAGTTTTGGACCTGTCACTTAATCGATTAAATGGCAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGATTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGCTCTGCACTACTTGGTCGTCCACCATGCGTAGTTCAAAAGCGAAAATATGAGATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCAGTTGCGGTAGTCTGTGTGCTGGTTGCTGTCTTTGTTTATTGTTTCTTCTACTTCAGAAAAAGGGATTCAAATCCAGAACACCCTGAACACAGGATGCTAAGAGTAATGGGATCACCGAGCCATCATGGAAGTCGAATTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGGGTTCAATGAGGCTCATCTCCTGGGTAGAGGAACCAGCGGAGCAGTATATAAAGCAGTTATTGACAATGGGAAAAATATCGTGGCAGTGAAGGTACTGCATGGTGATGGTAATCAGAGTTACAGAAGCTTCAAGAGGGAATGTCAAATCATGTCCCAAGTTAAGCATCGGAATCTTGTAAGAATAGTAGGATATACCTGGAACAAACAGTTCAAGGCTCTTATTCTCGAGTATATTGACAATGGTAACTTAGCACAGCATCTTTATCCCGGTGAACTGGAGGAAGGAGCATGTGAATTATCGTTGAGGGATAGAATGTCAATAGCTGTAGATGTTGCCAGTGGCTTGGAGTATCTTCAGGAAGGTTGTCCAGTCCAAATTATGCACTGTGATCTAAAACCAGAAAATGTGCTTATTGACAACAATATGGTGGCTCATGTAGCAGATTTCGGGATTGGGAAGATCAACGCAGCTGAGAAATCCAATGAATCACATGTTAGCACAACGCATTTTCTACGTGGATCAATTGGTTACATTCCACCAGAATATGGACAAGGAATTGAGGTATCAGCAAAAGGAGACATCTATAGCTTCGGTGTCATGGTGCTTGAGATGATAACAAGAAGAAGACCGACAAGCAATATGTTTCCGGATGGGCTTGATCTAAGGAAGTGGGTGGTTTCTTCGTTCCCAGACCATGTTTGGGATGTTATTGACGGTACGTTAAAGGAGAATGAGATACAAAGCACACATGGCGCTTTGGATGAGCTTGAGAAATGCTGCATTCAAATGCTTGATGTGGGGTTGATGTGCACAGAAGAGAATCCACAAGAAAGACCTGCTATGTCTTTTGTTGTGCAGAAGTTGAAACAGGTTATGAAAAGCTCCAATATCATTTGA

Protein Analysis

1041

Amino Acids

114.69

Weight (kDa)

6.77

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 2.7e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 144 - 256 7.3e-08 Leucine-rich repeat region
LRR_14 PF23598 340 - 478 3.8e-12 Leucine-rich repeat region
LRR_8 PF13855 377 - 435 6.2e-09 Leucine rich repeat
LRR_4 PF12799 401 - 435 3.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 423 - 483 2.1e-06 Leucine rich repeat
Pkinase PF00069 744 - 1024 6.3e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 747 - 1030 3.4e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1875
Acc36I ACCTGC 2 cut(s) 724, 3070
AccB7I CCANNNNNTGG 3 cut(s) 653, 1904, 2771
AccI GTMKAC 1 cut(s) 1171
AccII CGCG 1 cut(s) 747
AccIII TCCGGA 1 cut(s) 2872
AciI CCGC 6 cut(s) 561, 999, 1197, 1391, 2057, 2262
AclWI GGATC 4 cut(s) 143, 634, 2170, 2752
AcsI RAATTY 9 cut(s) 125, 351, 749, 858, 967, 1242, 1270, 1893, 2187
AcuI CTGAAG 3 cut(s) 774, 2089, 2564
AfaI GTAC 6 cut(s) 830, 1214, 1539, 1638, 2319, 2944
AfiI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
AflIII ACRYGT 3 cut(s) 831, 1722, 2716
AjnI CCWGG 3 cut(s) 1200, 2244, 2420
AjuI GAANNNNNNNTTGG 2 cut(s) 1378, 1410
Alw21I GWGCWC 2 cut(s) 404, 3038
Alw26I GTCTC 4 cut(s) 230, 545, 1575, 2796
Alw44I GTGCAC 1 cut(s) 3034
AlwI GGATC 4 cut(s) 143, 634, 2170, 2752
AlwNI CAGNNNCTG 1 cut(s) 1904
Ama87I CYCGRG 1 cut(s) 2450
Aor13HI TCCGGA 1 cut(s) 2872
AoxI GGCC 4 cut(s) 244, 1198, 1310, 1382
ApaLI GTGCAC 1 cut(s) 3034
ApeKI GCWGC 5 cut(s) 1495, 1698, 2048, 2693, 3003
ApoI RAATTY 9 cut(s) 125, 351, 749, 858, 967, 1242, 1270, 1893, 2187
AspLEI GCGC 1 cut(s) 2981
AspS9I GGNCC 3 cut(s) 1310, 1382, 1792
AsuC2I CCSGG 2 cut(s) 236, 2493
AsuHPI GGTGA 6 cut(s) 276, 1124, 2157, 2185, 2339, 2507
AsuII TTCGAA 3 cut(s) 1268, 1583, 1596
AvaI CYCGRG 1 cut(s) 2450
AvaII GGWCC 1 cut(s) 1792
BaeGI GKGCMC 1 cut(s) 3038
BanII GRGCYC 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 696, 728
BbsI GAAGAC 1 cut(s) 2857
Bbv12I GWGCWC 2 cut(s) 404, 3038
BbvI GCAGC 5 cut(s) 1482, 1685, 2060, 2705, 2990
BccI CCATC 7 cut(s) 79, 364, 1108, 1255, 2181, 2324, 2870
BciT130I CCWGG 3 cut(s) 1202, 2246, 2422
BclI TGATCA 2 cut(s) 1770, 1851
BcnI CCSGG 2 cut(s) 236, 2493
BcoDI GTCTC 4 cut(s) 230, 545, 1575, 2796
BfaI CTAG 5 cut(s) 102, 671, 953, 1355, 1437
BfmI CTRYAG 5 cut(s) 717, 936, 1064, 2550, 2809
BfoI RGCGCY 1 cut(s) 2982
BfuAI ACCTGC 2 cut(s) 724, 3070
BisI GCNGC 7 cut(s) 1000, 1198, 1496, 1699, 2049, 2694, 3004
BlsI GCNGC 7 cut(s) 1001, 1199, 1497, 1700, 2050, 2695, 3005
Bme1390I CCNGG 5 cut(s) 236, 1202, 2246, 2422, 2493
Bme18I GGWCC 1 cut(s) 1792
BmeT110I CYCGRG 1 cut(s) 2450
BmgT120I GGNCC 3 cut(s) 1310, 1382, 1792
BmiI GGNNCC 3 cut(s) 341, 1675, 2257
BmrFI CCNGG 5 cut(s) 236, 1202, 2246, 2422, 2493
BmrI ACTGGG 2 cut(s) 623, 1914
BmsI GCATC 5 cut(s) 1326, 1482, 2136, 2398, 2491
BmuI ACTGGG 2 cut(s) 623, 1914
BpiI GAAGAC 1 cut(s) 2857
BpmI CTGGAG 1 cut(s) 2522
Bpu14I TTCGAA 3 cut(s) 1268, 1583, 1596
BpuEI CTTGAG 4 cut(s) 794, 983, 2852, 3014
BpuMI CCSGG 2 cut(s) 236, 2493
Bsa29I ATCGAT 2 cut(s) 1807, 2027
BsaAI YACGTR 1 cut(s) 2741
BsaBI GATNNNNATC 2 cut(s) 2121, 2334
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BsaWI WCCGGW 1 cut(s) 2872
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
Bse3DI GCAATG 1 cut(s) 340
Bse8I GATNNNNATC 2 cut(s) 2121, 2334
BseAI TCCGGA 1 cut(s) 2872
BseBI CCWGG 3 cut(s) 1202, 2246, 2422
BseCI ATCGAT 2 cut(s) 1807, 2027
BseDI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BseGI GGATG 4 cut(s) 2151, 2881, 2935, 2992
BseJI GATNNNNATC 2 cut(s) 2121, 2334
BseLI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 4 cut(s) 571, 1033, 1409, 2688
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 3038
BseXI GCAGC 5 cut(s) 1482, 1685, 2060, 2705, 2990
BsgI GTGCAG 3 cut(s) 718, 1953, 3101
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 4 cut(s) 246, 1200, 1312, 1384
BshVI ATCGAT 2 cut(s) 1807, 2027
BsiHKAI GWGCWC 2 cut(s) 404, 3038
BsiHKCI CYCGRG 1 cut(s) 2450
BsiSI CCGG 3 cut(s) 235, 2493, 2873
BslFI GGGAC 2 cut(s) 1759, 2362
BslI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
BsmAI GTCTC 4 cut(s) 230, 545, 1575, 2796
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 1759, 2362
BsmI GAATGC 2 cut(s) 423, 3006
BsnI GGCC 4 cut(s) 246, 1200, 1312, 1384
Bso31I GGTCTC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 2450
Bsp119I TTCGAA 3 cut(s) 1268, 1583, 1596
Bsp1286I GDGCHC 2 cut(s) 404, 3038
Bsp13I TCCGGA 1 cut(s) 2872
Bsp1407I TGTACA 2 cut(s) 828, 1537
BspACI CCGC 6 cut(s) 561, 999, 1197, 1391, 2057, 2262
BspANI GGCC 4 cut(s) 246, 1200, 1312, 1384
BspCNI CTCAG 4 cut(s) 570, 1032, 1410, 2689
BspDI ATCGAT 2 cut(s) 1807, 2027
BspEI TCCGGA 1 cut(s) 2872
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 3 cut(s) 341, 1675, 2257
BspMAI CTGCAG 2 cut(s) 721, 940
BspMI ACCTGC 2 cut(s) 724, 3070
BspPI GGATC 4 cut(s) 143, 634, 2170, 2752
BspT104I TTCGAA 3 cut(s) 1268, 1583, 1596
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 340
BsrGI TGTACA 2 cut(s) 828, 1537
BssECI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BssNAI GTATAC 1 cut(s) 1172
BssT1I CCWWGG 2 cut(s) 363, 1345
Bst1107I GTATAC 1 cut(s) 1172
Bst2UI CCWGG 3 cut(s) 1202, 2246, 2422
Bst4CI ACNGT 9 cut(s) 482, 897, 1046, 1068, 1229, 1605, 2433, 2612, 2942
Bst6I CTCTTC 2 cut(s) 312, 3036
BstAPI GCANNNNNTGC 1 cut(s) 2054
BstAUI TGTACA 2 cut(s) 828, 1537
BstBAI YACGTR 1 cut(s) 2741
BstBI TTCGAA 3 cut(s) 1268, 1583, 1596
BstC8I GCNNGC 5 cut(s) 269, 950, 1262, 1611, 1615
BstDEI CTNAG 8 cut(s) 75, 557, 1019, 1418, 2150, 2473, 2697, 2888
BstDSI CCRYGG 1 cut(s) 343
BstEII GGTNACC 2 cut(s) 1112, 1204
BstF5I GGATG 4 cut(s) 2151, 2881, 2935, 2992
BstFNI CGCG 1 cut(s) 747
BstH2I RGCGCY 1 cut(s) 2982
BstHHI GCGC 1 cut(s) 2981
BstMAI GTCTC 4 cut(s) 230, 545, 1575, 2796
BstMWI GCNNNNNNNGC 4 cut(s) 1390, 2054, 2663, 2976
BstNI CCWGG 3 cut(s) 1202, 2246, 2422
BstNSI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
BstPI GGTNACC 2 cut(s) 1112, 1204
BstSCI CCNGG 5 cut(s) 234, 1200, 2244, 2420, 2491
BstSFI CTRYAG 5 cut(s) 717, 936, 1064, 2550, 2809
BstSLI GKGCMC 1 cut(s) 3038
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 5 cut(s) 1482, 1685, 2060, 2705, 2990
BstV2I GAAGAC 1 cut(s) 2857
BstX2I RGATCY 1 cut(s) 639
BstXI CCANNNNNNTGG 3 cut(s) 991, 1670, 2569
BstYI RGATCY 1 cut(s) 639
BstZ17I GTATAC 1 cut(s) 1172
Bsu15I ATCGAT 2 cut(s) 1807, 2027
BsuRI GGCC 4 cut(s) 246, 1200, 1312, 1384
BsuTUI ATCGAT 2 cut(s) 1807, 2027
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 4 cut(s) 2151, 2881, 2935, 2992
BtsI GCAGTG 3 cut(s) 735, 1477, 2316
BtsIMutI CAGTG 8 cut(s) 735, 870, 1086, 1477, 1902, 2316, 2569, 2608
BveI ACCTGC 2 cut(s) 724, 3070
Cac8I GCNNGC 5 cut(s) 269, 950, 1262, 1611, 1615
CaiI CAGNNNCTG 1 cut(s) 1904
CfoI GCGC 1 cut(s) 2981
Cfr13I GGNCC 3 cut(s) 1310, 1382, 1792
ClaI ATCGAT 2 cut(s) 1807, 2027
Csp6I GTAC 6 cut(s) 829, 1213, 1538, 1637, 2318, 2943
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 6 cut(s) 829, 1213, 1538, 1637, 2318, 2943
DdeI CTNAG 8 cut(s) 75, 557, 1019, 1418, 2150, 2473, 2697, 2888
Eam1104I CTCTTC 2 cut(s) 312, 3036
EarI CTCTTC 2 cut(s) 312, 3036
EciI GGCGGA 1 cut(s) 1380
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 363, 1345
Eco147I AGGCCT 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 1 cut(s) 545
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 1792
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 3 cut(s) 774, 2089, 2564
Eco88I CYCGRG 1 cut(s) 2450
Eco91I GGTNACC 2 cut(s) 1112, 1204
EcoICRI GAGCTC 1 cut(s) 402
EcoO65I GGTNACC 2 cut(s) 1112, 1204
EcoRI GAATTC 2 cut(s) 749, 858
EcoRII CCWGG 3 cut(s) 1200, 2244, 2420
EcoRV GATATC 1 cut(s) 282
EcoT14I CCWWGG 2 cut(s) 363, 1345
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 2 cut(s) 363, 1345
Esp3I CGTCTC 1 cut(s) 230
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 2 cut(s) 1759, 2362
FbaI TGATCA 2 cut(s) 1770, 1851
FblI GTMKAC 1 cut(s) 1171
Fnu4HI GCNGC 7 cut(s) 1000, 1198, 1496, 1699, 2049, 2694, 3004
FokI GGATG 4 cut(s) 2158, 2888, 2942, 2999
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 7 cut(s) 1000, 1198, 1496, 1699, 2049, 2694, 3004
FspBI CTAG 5 cut(s) 102, 671, 953, 1355, 1437
GlaI GCGC 1 cut(s) 2980
GluI GCNGC 7 cut(s) 1000, 1198, 1496, 1699, 2049, 2694, 3004
GsuI CTGGAG 1 cut(s) 2522
HaeII RGCGCY 1 cut(s) 2982
HaeIII GGCC 4 cut(s) 246, 1200, 1312, 1384
HapII CCGG 3 cut(s) 235, 2493, 2873
HhaI GCGC 1 cut(s) 2981
Hin6I GCGC 1 cut(s) 2979
HinP1I GCGC 1 cut(s) 2979
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 5 cut(s) 410, 451, 845, 1262, 2350
HpaII CCGG 3 cut(s) 235, 2493, 2873
HphI GGTGA 6 cut(s) 276, 1124, 2157, 2185, 2339, 2507
Hpy166II GTNNAC 8 cut(s) 550, 622, 1086, 1172, 1352, 1985, 2498, 3036
Hpy8I GTNNAC 8 cut(s) 550, 622, 1086, 1172, 1352, 1985, 2498, 3036
HpyCH4III ACNGT 9 cut(s) 482, 897, 1046, 1068, 1229, 1605, 2433, 2612, 2942
HpyCH4IV ACGT 3 cut(s) 223, 2740, 2945
HpyF10VI GCNNNNNNNGC 4 cut(s) 1390, 2054, 2663, 2976
HpyF3I CTNAG 8 cut(s) 75, 557, 1019, 1418, 2150, 2473, 2697, 2888
HpySE526I ACGT 3 cut(s) 223, 2740, 2945
HspAI GCGC 1 cut(s) 2979
Kpn2I TCCGGA 1 cut(s) 2872
Ksp22I TGATCA 2 cut(s) 1770, 1851
LmnI GCTCC 6 cut(s) 136, 656, 1679, 2264, 2510, 3113
Lsp1109I GCAGC 5 cut(s) 1482, 1685, 2060, 2705, 2990
LweI GCATC 5 cut(s) 1326, 1482, 2136, 2398, 2491
MaeI CTAG 5 cut(s) 102, 671, 953, 1355, 1437
MaeII ACGT 3 cut(s) 223, 2740, 2945
MaeIII GTNAC 8 cut(s) 728, 865, 1112, 1204, 1797, 2342, 2468, 2753
MfeI CAATTG 2 cut(s) 1644, 2748
MflI RGATCY 1 cut(s) 639
MhlI GDGCHC 2 cut(s) 404, 3038
MlyI GAGTC 1 cut(s) 1766
MmeI TCCRAC 3 cut(s) 321, 1008, 1752
MroI TCCGGA 1 cut(s) 2872
MslI CAYNNNNRTG 3 cut(s) 467, 2328, 3011
MspA1I CMGCKG 5 cut(s) 561, 889, 1417, 2262, 2696
MspI CCGG 3 cut(s) 235, 2493, 2873
MspR9I CCNGG 5 cut(s) 236, 1202, 2246, 2422, 2493
MunI CAATTG 2 cut(s) 1644, 2748
Mva1269I GAATGC 2 cut(s) 423, 3006
MvaI CCWGG 3 cut(s) 1202, 2246, 2422
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 4 cut(s) 1390, 2054, 2663, 2976
NciI CCSGG 2 cut(s) 236, 2493
NlaIV GGNNCC 3 cut(s) 341, 1675, 2257
NmeAIII GCCGAG 1 cut(s) 489
NmuCI GTSAC 3 cut(s) 865, 1112, 1797
NspI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
NspV TTCGAA 3 cut(s) 1268, 1583, 1596
PaeR7I CTCGAG 1 cut(s) 2450
PceI AGGCCT 1 cut(s) 246
PciI ACATGT 3 cut(s) 831, 1722, 2716
PctI GAATGC 2 cut(s) 423, 3006
PfeI GAWTC 9 cut(s) 98, 523, 676, 1141, 1858, 2117, 2395, 2711, 3046
PflMI CCANNNNNTGG 3 cut(s) 653, 1904, 2771
PkrI GCNGC 7 cut(s) 1001, 1199, 1497, 1700, 2050, 2695, 3005
PleI GAGTC 1 cut(s) 1765
PpsI GAGTC 1 cut(s) 1765
Ppu21I YACGTR 1 cut(s) 2741
PscI ACATGT 3 cut(s) 831, 1722, 2716
PsiI TTATAA 1 cut(s) 1875
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 1200, 2244, 2420
PspEI GGTNACC 2 cut(s) 1112, 1204
PspGI CCWGG 3 cut(s) 1200, 2244, 2420
PspN4I GGNNCC 3 cut(s) 341, 1675, 2257
PspPI GGNCC 3 cut(s) 1310, 1382, 1792
PstI CTGCAG 2 cut(s) 721, 940
PstNI CAGNNNCTG 1 cut(s) 1904
PsuI RGATCY 1 cut(s) 639
PvuII CAGCTG 3 cut(s) 889, 1417, 2696
RsaI GTAC 6 cut(s) 830, 1214, 1539, 1638, 2319, 2944
RsaNI GTAC 6 cut(s) 829, 1213, 1538, 1637, 2318, 2943
RseI CAYNNNNRTG 3 cut(s) 467, 2328, 3011
SacI GAGCTC 1 cut(s) 404
SatI GCNGC 7 cut(s) 1000, 1198, 1496, 1699, 2049, 2694, 3004
Sau96I GGNCC 3 cut(s) 1310, 1382, 1792
SbfI CCTGCAGG 1 cut(s) 940
SchI GAGTC 1 cut(s) 1766
ScrFI CCNGG 5 cut(s) 236, 1202, 2246, 2422, 2493
SdaI CCTGCAGG 1 cut(s) 940
SduI GDGCHC 2 cut(s) 404, 3038
SfaNI GCATC 5 cut(s) 1326, 1482, 2136, 2398, 2491
SfcI CTRYAG 5 cut(s) 717, 936, 1064, 2550, 2809
Sfr274I CTCGAG 1 cut(s) 2450
SfuI TTCGAA 3 cut(s) 1268, 1583, 1596
SinI GGWCC 1 cut(s) 1792
SlaI CTCGAG 1 cut(s) 2450
SmiMI CAYNNNNRTG 3 cut(s) 467, 2328, 3011
SmlI CTYRAG 5 cut(s) 809, 962, 2450, 2831, 2993
SmoI CTYRAG 5 cut(s) 809, 962, 2450, 2831, 2993
Sse8387I CCTGCAGG 1 cut(s) 940
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 6 cut(s) 561, 999, 1197, 1391, 2057, 2262
SspMI CTAG 5 cut(s) 102, 671, 953, 1355, 1437
SstI GAGCTC 1 cut(s) 404
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 5 cut(s) 234, 1200, 2244, 2420, 2491
StyI CCWWGG 2 cut(s) 363, 1345
TaaI ACNGT 9 cut(s) 482, 897, 1046, 1068, 1229, 1605, 2433, 2612, 2942
TaiI ACGT 3 cut(s) 226, 2743, 2948
TaqI TCGA 9 cut(s) 702, 1268, 1373, 1583, 1596, 1807, 2027, 2185, 2451
TaqII GACCGA 1 cut(s) 2869
TatI WGTACW 4 cut(s) 828, 1212, 1537, 1636
TauI GCSGC 2 cut(s) 1002, 1200
TfiI GAWTC 9 cut(s) 98, 523, 676, 1141, 1858, 2117, 2395, 2711, 3046
TscAI CASTG 8 cut(s) 742, 870, 1093, 1484, 1909, 2316, 2569, 2615
TseFI GTSAC 3 cut(s) 865, 1112, 1797
TseI GCWGC 5 cut(s) 1495, 1698, 2048, 2693, 3003
Tsp45I GTSAC 3 cut(s) 865, 1112, 1797
TspDTI ATGAA 8 cut(s) 43, 280, 423, 485, 533, 1038, 2724, 3116
TspGWI ACGGA 2 cut(s) 360, 535
TspRI CASTG 8 cut(s) 742, 870, 1093, 1484, 1909, 2316, 2569, 2615
Van91I CCANNNNNTGG 3 cut(s) 653, 1904, 2771
VneI GTGCAC 1 cut(s) 3034
VpaK11BI GGWCC 1 cut(s) 1792
XapI RAATTY 9 cut(s) 125, 351, 749, 858, 967, 1242, 1270, 1893, 2187
XbaI TCTAGA 1 cut(s) 1436
XceI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
XcmI CCANNNNNNNNNTGG 1 cut(s) 2922
XhoI CTCGAG 1 cut(s) 2450
XmiI GTMKAC 1 cut(s) 1171
XspI CTAG 5 cut(s) 102, 671, 953, 1355, 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.