Rh3DG064400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0013738
Physical Location & Seq
Reverse (-)
4266700 .. 4270395
3696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG064400.1

Sequence Viewer

Length: 3111 bp
ATGAACAAAGAAGCCAGAGAAAGAATGGCATACAATCTGAACTATTGTATAACAGTAATGGTACTATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAAGTCTGCATTTCCTTGTGAGGATTCCAGTGATTGCAACACTCTGCTCAAATTCAAGGAGGAGCTGACAAGTGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCTTCTGCAACTGGACTGGAATTACATGCCACCAAAGTCTCCAAAACCGGGTTATAGGCCTTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAACCTCTCTCTTCTTACCAACCTGTCTTTACAGAGCAATGGGTTCCATGGACAAATTCCATCTTCCTTGGGAATACTTAAAGAGTTGGCATTTCTGAATGTGAGCTCAAACTGGCTTGAAGGTAGCATTCCAGGCTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACTTGACTTACAACAGTCTATCTGGTGTTCTTCCTAAAGAACTCGGCTGGATGAAGAATCTAACGTACTTGGCTCTCTCCGCAAACAATCTCAGCGGACTGCTTCCAGCCTCGCTTCCAAACTTGACGGAATTGACACAACTAGAACTGGCTGGCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTTTGTATCTCCATAAAAATTATCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTATGCGAAATCACGCTCATTGAGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAACAACAAGCTTTCTGGGAGAATTCCAGTGACCCTCTCCAATCTTTCGCAGCTGATACTGTTTGATGTTAGTTTGAATTATTTAGAGGGGGAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATTCCAATAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTAACTGCTCTAACTAACTGTTCATATCTGCGAAAACTACACTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTGGATAACCACATTACAGGAAGTATACCAGATAGTATCGGAAACTTAAGCGGCCTGGTAACCTTGAACATGTCATACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAAAAGACTTGGGGATGATGCCTAACCTTGGTTTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCGATTGAGCTTACTCAATGTTCTCTTATGATGCTGCTTGATCTATCGTTCAACAACTTAAAAGGCTCTGTTCCTGTACAGATTGACCGTTTTTCCAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAAGTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATTTGTACTGGCAATTGACTTGTCTGACAACCAGTTTTCTGGGTCCATACCTACATCGATCGGAAGCTGCATATCTTTGGCGTATCTAAACATGTCCAACAACATAATTGAGGGGACTATTCCAGAGTCGTTGAAGCTGATCACTCAACTTGAAGTTTTGGACTTGTCACTTAATCGATTAAATGGCAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGGTTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCTGCACTACTTGGTCGTCCGCCATGCGTAGTTCAAAAGAGAAAATATAAGATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCAGTTGCAGTAGTCTGTGCTCTGGTTGCTGTCTTTGTTTATTGTTTCTTCTTCTTCAGAAAACGGGATTCAAAACCAGAACACCGTGAACACAGAATGCTAAGAGTAATGGGATCACCAAGCCATCATGGAAGTCGAATTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGAGTTCAATGAGGTTCATCTCCTAGGTAGAGGAACCAGCGGAGCAGTCTATAAAGCAGTAATTGACAATGGGGAAAATATCGTGGCAGTGAAGGTACTGCATGGTGATGGTAATCAGAGTTACAGAAGCTTCAAGAGGGAATGCCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTAAGAATGGTAGGATATACCTGGAACAAACAGTTCAAGGCTCTTATTCTCGAGTATATTGACAATGGTAACTTAGCACAGCATCTTTATCCTGGTGAACTGGAGGAAGGAGCATGTGAATTATCGTTGAGGGATAGAATGTCAATAGCTATAGATGTTGCCAGTGGCTTGGAGTATCTTCAGGAAGGTTGTCCAGTCCAAATTATGCACTGTGATCTAAAACCAGAAAATGTGCTTATTGACAACAATATGGTGGCTCATGTAGCAGATTTCGGGATTGGGAAGCTCAACGCAGCTGAGAAATCCAACGAATCACATGTTAGCACAACACATTTTCTACGTGGATCAATTGGTTACATTCCCCCAGAATATGGACAAGGAATTGAGGTATCAGCAAAAGGAGACATCTATAGCTTCGGTGTCATGGTGCTTGAGATGATAACAAGAAGAAGACCGACAAGCAATATGTTTCCGGATGGGCTTGATCTAAGGAAGTGGGTGGTTTCTTCGTTCCCAGACCATGTTTGGGATGTTGTTGACAGTACATTAAAGGAGATACAAAGCACACATGGCGCTTTTGATGAGCTTGAGAAATGCTGCATTCAAATGCTTGATGTGGGGTTGATGTGCACAGAAGAGAATCCACAAGAAAGACCTGCTATATGTCTTTTGTTGTGCAGAAGTTGA

Protein Analysis

1036

Amino Acids

114.52

Weight (kDa)

6.64

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 80 8e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 153 - 263 4.7e-09 Leucine-rich repeat region
LRR_8 PF13855 159 - 217 5.1e-07 Leucine rich repeat
LRR_14 PF23598 348 - 461 3.6e-11 Leucine-rich repeat region
LRR_8 PF13855 385 - 443 3.4e-08 Leucine rich repeat
Pkinase PF00069 752 - 973 8.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 755 - 1029 3.1e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1899
Acc36I ACCTGC 2 cut(s) 748, 3088
AccB7I CCANNNNNTGG 3 cut(s) 677, 1928, 2795
AccI GTMKAC 1 cut(s) 1195
AccIII TCCGGA 1 cut(s) 2896
AciI CCGC 7 cut(s) 570, 585, 1023, 1221, 1415, 2009, 2286
AclWI GGATC 4 cut(s) 167, 658, 2194, 2776
AcsI RAATTY 9 cut(s) 149, 375, 882, 991, 1266, 1294, 1382, 1917, 2211
AcuI CTGAAG 3 cut(s) 798, 2113, 2588
AfaI GTAC 7 cut(s) 63, 557, 854, 1563, 1662, 2343, 2968
AfiI CCNNNNNNNGG 7 cut(s) 259, 677, 1370, 1424, 1928, 2795, 2950
AflII CTTAAG 1 cut(s) 1216
AflIII ACRYGT 4 cut(s) 855, 1239, 1746, 2740
AjnI CCWGG 4 cut(s) 451, 1224, 2444, 2515
AjuI GAANNNNNNNTTGG 2 cut(s) 1402, 1434
Alw21I GWGCWC 3 cut(s) 428, 2095, 3056
Alw26I GTCTC 2 cut(s) 254, 2820
Alw44I GTGCAC 1 cut(s) 3052
AlwI GGATC 4 cut(s) 167, 658, 2194, 2776
AlwNI CAGNNNCTG 1 cut(s) 1928
Ama87I CYCGRG 1 cut(s) 2474
Aor13HI TCCGGA 1 cut(s) 2896
AoxI GGCC 4 cut(s) 268, 1222, 1334, 1406
ApaLI GTGCAC 1 cut(s) 3052
ApeKI GCWGC 6 cut(s) 910, 1519, 1722, 2072, 2717, 3021
ApoI RAATTY 9 cut(s) 149, 375, 882, 991, 1266, 1294, 1382, 1917, 2211
AspA2I CCTAGG 1 cut(s) 2269
AspLEI GCGC 1 cut(s) 2999
AspS9I GGNCC 3 cut(s) 1334, 1406, 1698
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 7 cut(s) 300, 1148, 2181, 2209, 2258, 2363, 2531
AsuII TTCGAA 3 cut(s) 1292, 1607, 1620
AvaI CYCGRG 1 cut(s) 2474
AvaII GGWCC 1 cut(s) 1698
AvrII CCTAGG 1 cut(s) 2269
BaeGI GKGCMC 1 cut(s) 3056
BanII GRGCYC 1 cut(s) 428
BarI GAAGNNNNNNTAC 2 cut(s) 720, 752
BbsI GAAGAC 1 cut(s) 2881
Bbv12I GWGCWC 3 cut(s) 428, 2095, 3056
BbvI GCAGC 6 cut(s) 922, 1506, 1709, 2084, 2729, 3008
BccI CCATC 7 cut(s) 103, 388, 1132, 1279, 2205, 2348, 2894
BciT130I CCWGG 4 cut(s) 453, 1226, 2446, 2517
BclI TGATCA 2 cut(s) 1794, 1875
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 2 cut(s) 254, 2820
BfaI CTAG 6 cut(s) 632, 695, 977, 1379, 1461, 2270
BfmI CTRYAG 4 cut(s) 741, 960, 2574, 2833
BfoI RGCGCY 1 cut(s) 3000
BfrI CTTAAG 1 cut(s) 1216
BfuAI ACCTGC 2 cut(s) 748, 3088
BisI GCNGC 8 cut(s) 911, 1024, 1222, 1520, 1723, 2073, 2718, 3022
BlnI CCTAGG 1 cut(s) 2269
BlsI GCNGC 8 cut(s) 912, 1025, 1223, 1521, 1724, 2074, 2719, 3023
Bme1390I CCNGG 5 cut(s) 260, 453, 1226, 2446, 2517
Bme18I GGWCC 1 cut(s) 1698
BmeT110I CYCGRG 1 cut(s) 2474
BmgT120I GGNCC 3 cut(s) 1334, 1406, 1698
BmiI GGNNCC 3 cut(s) 365, 1699, 2281
BmrFI CCNGG 5 cut(s) 260, 453, 1226, 2446, 2517
BmrI ACTGGG 1 cut(s) 1938
BmsI GCATC 3 cut(s) 1350, 1506, 2515
BmuI ACTGGG 1 cut(s) 1938
BpiI GAAGAC 1 cut(s) 2881
BpmI CTGGAG 1 cut(s) 2546
Bpu14I TTCGAA 3 cut(s) 1292, 1607, 1620
BpuEI CTTGAG 4 cut(s) 818, 1007, 2876, 3032
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 3 cut(s) 1712, 1831, 2051
BsaAI YACGTR 1 cut(s) 2765
BsaBI GATNNNNATC 1 cut(s) 2358
BsaJI CCNNGG 4 cut(s) 367, 387, 1369, 2269
BsaWI WCCGGW 2 cut(s) 2415, 2896
Bsc4I CCNNNNNNNGG 7 cut(s) 259, 677, 1370, 1424, 1928, 2795, 2950
Bse3DI GCAATG 1 cut(s) 364
Bse8I GATNNNNATC 1 cut(s) 2358
BseAI TCCGGA 1 cut(s) 2896
BseBI CCWGG 4 cut(s) 453, 1226, 2446, 2517
BseCI ATCGAT 3 cut(s) 1712, 1831, 2051
BseDI CCNNGG 4 cut(s) 367, 387, 1369, 2269
BseGI GGATG 4 cut(s) 546, 1362, 2905, 2959
BseJI GATNNNNATC 1 cut(s) 2358
BseLI CCNNNNNNNGG 7 cut(s) 259, 677, 1370, 1424, 1928, 2795, 2950
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 4 cut(s) 595, 1057, 1433, 2712
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 3056
BseXI GCAGC 6 cut(s) 922, 1506, 1709, 2084, 2729, 3008
BsgI GTGCAG 2 cut(s) 742, 1977
Bsh1285I CGRYCG 1 cut(s) 1716
BshFI GGCC 4 cut(s) 270, 1224, 1336, 1408
BshVI ATCGAT 3 cut(s) 1712, 1831, 2051
BsiEI CGRYCG 1 cut(s) 1716
BsiHKAI GWGCWC 3 cut(s) 428, 2095, 3056
BsiHKCI CYCGRG 1 cut(s) 2474
BsiSI CCGG 3 cut(s) 259, 2416, 2897
BslFI GGGAC 1 cut(s) 1783
BslI CCNNNNNNNGG 7 cut(s) 259, 677, 1370, 1424, 1928, 2795, 2950
BsmAI GTCTC 2 cut(s) 254, 2820
BsmFI GGGAC 1 cut(s) 1783
BsmI GAATGC 4 cut(s) 447, 2175, 2393, 3024
BsnI GGCC 4 cut(s) 270, 1224, 1336, 1408
BsoBI CYCGRG 1 cut(s) 2474
Bsp119I TTCGAA 3 cut(s) 1292, 1607, 1620
Bsp1286I GDGCHC 3 cut(s) 428, 2095, 3056
Bsp13I TCCGGA 1 cut(s) 2896
Bsp1407I TGTACA 2 cut(s) 852, 1561
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 7 cut(s) 570, 585, 1023, 1221, 1415, 2009, 2286
BspANI GGCC 4 cut(s) 270, 1224, 1336, 1408
BspCNI CTCAG 4 cut(s) 594, 1056, 1434, 2713
BspDI ATCGAT 3 cut(s) 1712, 1831, 2051
BspEI TCCGGA 1 cut(s) 2896
BspLI GGNNCC 3 cut(s) 365, 1699, 2281
BspMAI CTGCAG 2 cut(s) 745, 964
BspMI ACCTGC 2 cut(s) 748, 3088
BspPI GGATC 4 cut(s) 167, 658, 2194, 2776
BspT104I TTCGAA 3 cut(s) 1292, 1607, 1620
BspTI CTTAAG 1 cut(s) 1216
BsrDI GCAATG 1 cut(s) 364
BsrGI TGTACA 2 cut(s) 852, 1561
BssECI CCNNGG 4 cut(s) 367, 387, 1369, 2269
BssNAI GTATAC 1 cut(s) 1196
BssT1I CCWWGG 4 cut(s) 367, 387, 1369, 2269
Bst1107I GTATAC 1 cut(s) 1196
Bst2UI CCWGG 4 cut(s) 453, 1226, 2446, 2517
Bst6I CTCTTC 2 cut(s) 336, 3054
BstAFI CTTAAG 1 cut(s) 1216
BstAPI GCANNNNNTGC 1 cut(s) 2078
BstAUI TGTACA 2 cut(s) 852, 1561
BstBAI YACGTR 1 cut(s) 2765
BstBI TTCGAA 3 cut(s) 1292, 1607, 1620
BstC8I GCNNGC 6 cut(s) 293, 643, 974, 1286, 1635, 1639
BstDEI CTNAG 8 cut(s) 99, 581, 1043, 1442, 2174, 2497, 2721, 2912
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 2 cut(s) 1136, 1228
BstF5I GGATG 4 cut(s) 546, 1362, 2905, 2959
BstH2I RGCGCY 1 cut(s) 3000
BstHHI GCGC 1 cut(s) 2999
BstMAI GTCTC 2 cut(s) 254, 2820
BstMCI CGRYCG 1 cut(s) 1716
BstMWI GCNNNNNNNGC 7 cut(s) 453, 569, 1414, 2078, 2099, 2687, 2994
BstNI CCWGG 4 cut(s) 453, 1226, 2446, 2517
BstNSI RCATGY 6 cut(s) 240, 859, 1243, 1750, 2541, 2744
BstPI GGTNACC 2 cut(s) 1136, 1228
BstSCI CCNGG 5 cut(s) 258, 451, 1224, 2444, 2515
BstSFI CTRYAG 4 cut(s) 741, 960, 2574, 2833
BstSLI GKGCMC 1 cut(s) 3056
BstV1I GCAGC 6 cut(s) 922, 1506, 1709, 2084, 2729, 3008
BstV2I GAAGAC 1 cut(s) 2881
BstX2I RGATCY 1 cut(s) 663
BstXI CCANNNNNNTGG 3 cut(s) 1015, 1694, 2593
BstYI RGATCY 1 cut(s) 663
BstZ17I GTATAC 1 cut(s) 1196
Bsu15I ATCGAT 3 cut(s) 1712, 1831, 2051
BsuRI GGCC 4 cut(s) 270, 1224, 1336, 1408
BsuTUI ATCGAT 3 cut(s) 1712, 1831, 2051
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 4 cut(s) 546, 1362, 2905, 2959
BtsI GCAGTG 2 cut(s) 759, 2340
BtsIMutI CAGTG 8 cut(s) 133, 759, 894, 1110, 1926, 2340, 2593, 2632
BveI ACCTGC 2 cut(s) 748, 3088
Cac8I GCNNGC 6 cut(s) 293, 643, 974, 1286, 1635, 1639
CaiI CAGNNNCTG 1 cut(s) 1928
CfoI GCGC 1 cut(s) 2999
Cfr13I GGNCC 3 cut(s) 1334, 1406, 1698
ClaI ATCGAT 3 cut(s) 1712, 1831, 2051
Csp6I GTAC 7 cut(s) 62, 556, 853, 1562, 1661, 2342, 2967
CviQI GTAC 7 cut(s) 62, 556, 853, 1562, 1661, 2342, 2967
DdeI CTNAG 8 cut(s) 99, 581, 1043, 1442, 2174, 2497, 2721, 2912
Eam1104I CTCTTC 2 cut(s) 336, 3054
EarI CTCTTC 2 cut(s) 336, 3054
EciI GGCGGA 2 cut(s) 1404, 1998
Ecl136II GAGCTC 1 cut(s) 426
Eco130I CCWWGG 4 cut(s) 367, 387, 1369, 2269
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 428
Eco32I GATATC 2 cut(s) 306, 1448
Eco47I GGWCC 1 cut(s) 1698
Eco53kI GAGCTC 1 cut(s) 426
Eco57I CTGAAG 3 cut(s) 798, 2113, 2588
Eco88I CYCGRG 1 cut(s) 2474
Eco91I GGTNACC 2 cut(s) 1136, 1228
EcoICRI GAGCTC 1 cut(s) 426
EcoO65I GGTNACC 2 cut(s) 1136, 1228
EcoRI GAATTC 1 cut(s) 882
EcoRII CCWGG 4 cut(s) 451, 1224, 2444, 2515
EcoRV GATATC 2 cut(s) 306, 1448
EcoT14I CCWWGG 4 cut(s) 367, 387, 1369, 2269
EcoT38I GRGCYC 1 cut(s) 428
ErhI CCWWGG 4 cut(s) 367, 387, 1369, 2269
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 1 cut(s) 1783
FbaI TGATCA 2 cut(s) 1794, 1875
FblI GTMKAC 1 cut(s) 1195
Fnu4HI GCNGC 8 cut(s) 911, 1024, 1222, 1520, 1723, 2073, 2718, 3022
FokI GGATG 4 cut(s) 553, 1369, 2912, 2966
FriOI GRGCYC 1 cut(s) 428
Fsp4HI GCNGC 8 cut(s) 911, 1024, 1222, 1520, 1723, 2073, 2718, 3022
FspBI CTAG 6 cut(s) 632, 695, 977, 1379, 1461, 2270
GlaI GCGC 1 cut(s) 2998
GluI GCNGC 8 cut(s) 911, 1024, 1222, 1520, 1723, 2073, 2718, 3022
GsuI CTGGAG 1 cut(s) 2546
HaeII RGCGCY 1 cut(s) 3000
HaeIII GGCC 4 cut(s) 270, 1224, 1336, 1408
HapII CCGG 3 cut(s) 259, 2416, 2897
HhaI GCGC 1 cut(s) 2999
Hin6I GCGC 1 cut(s) 2997
HinP1I GCGC 1 cut(s) 2997
HincII GTYRAC 1 cut(s) 2962
HindII GTYRAC 1 cut(s) 2962
HindIII AAGCTT 4 cut(s) 475, 869, 1286, 2374
HinfI GANTC 9 cut(s) 122, 547, 1165, 1781, 1882, 2141, 2419, 2735, 3064
HpaII CCGG 3 cut(s) 259, 2416, 2897
HphI GGTGA 7 cut(s) 300, 1148, 2181, 2209, 2258, 2363, 2531
Hpy166II GTNNAC 7 cut(s) 1110, 1196, 1376, 2162, 2522, 2962, 3054
Hpy8I GTNNAC 7 cut(s) 1110, 1196, 1376, 2162, 2522, 2962, 3054
HpyCH4IV ACGT 2 cut(s) 554, 2764
HpyF10VI GCNNNNNNNGC 7 cut(s) 453, 569, 1414, 2078, 2099, 2687, 2994
HpyF3I CTNAG 8 cut(s) 99, 581, 1043, 1442, 2174, 2497, 2721, 2912
HpySE526I ACGT 2 cut(s) 554, 2764
HspAI GCGC 1 cut(s) 2997
Kpn2I TCCGGA 1 cut(s) 2896
Ksp22I TGATCA 2 cut(s) 1794, 1875
LmnI GCTCC 4 cut(s) 160, 680, 2288, 2534
Lsp1109I GCAGC 6 cut(s) 922, 1506, 1709, 2084, 2729, 3008
LweI GCATC 3 cut(s) 1350, 1506, 2515
MaeI CTAG 6 cut(s) 632, 695, 977, 1379, 1461, 2270
MaeII ACGT 2 cut(s) 554, 2764
MaeIII GTNAC 8 cut(s) 752, 889, 1136, 1228, 1821, 2366, 2492, 2777
MfeI CAATTG 2 cut(s) 1668, 2772
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 3 cut(s) 428, 2095, 3056
MlyI GAGTC 1 cut(s) 1790
MmeI TCCRAC 3 cut(s) 345, 1776, 2754
MroI TCCGGA 1 cut(s) 2896
MslI CAYNNNNRTG 2 cut(s) 2352, 3029
MspA1I CMGCKG 5 cut(s) 585, 913, 1441, 2286, 2720
MspCI CTTAAG 1 cut(s) 1216
MspI CCGG 3 cut(s) 259, 2416, 2897
MspR9I CCNGG 5 cut(s) 260, 453, 1226, 2446, 2517
MunI CAATTG 2 cut(s) 1668, 2772
Mva1269I GAATGC 4 cut(s) 447, 2175, 2393, 3024
MvaI CCWGG 4 cut(s) 453, 1226, 2446, 2517
MwoI GCNNNNNNNGC 7 cut(s) 453, 569, 1414, 2078, 2099, 2687, 2994
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 367
NlaIV GGNNCC 3 cut(s) 365, 1699, 2281
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 3 cut(s) 889, 1136, 1821
NspI RCATGY 6 cut(s) 240, 859, 1243, 1750, 2541, 2744
NspV TTCGAA 3 cut(s) 1292, 1607, 1620
PaeR7I CTCGAG 1 cut(s) 2474
PceI AGGCCT 1 cut(s) 270
PciI ACATGT 4 cut(s) 855, 1239, 1746, 2740
PctI GAATGC 4 cut(s) 447, 2175, 2393, 3024
PfeI GAWTC 8 cut(s) 122, 547, 1165, 1882, 2141, 2419, 2735, 3064
PflMI CCANNNNNTGG 3 cut(s) 677, 1928, 2795
PkrI GCNGC 8 cut(s) 912, 1025, 1223, 1521, 1724, 2074, 2719, 3023
Ple19I CGATCG 1 cut(s) 1716
PleI GAGTC 1 cut(s) 1789
PpsI GAGTC 1 cut(s) 1789
Ppu21I YACGTR 1 cut(s) 2765
PscI ACATGT 4 cut(s) 855, 1239, 1746, 2740
PsiI TTATAA 1 cut(s) 1899
Psp124BI GAGCTC 1 cut(s) 428
Psp6I CCWGG 4 cut(s) 451, 1224, 2444, 2515
PspEI GGTNACC 2 cut(s) 1136, 1228
PspGI CCWGG 4 cut(s) 451, 1224, 2444, 2515
PspN4I GGNNCC 3 cut(s) 365, 1699, 2281
PspPI GGNCC 3 cut(s) 1334, 1406, 1698
PsrI GAACNNNNNNTAC 2 cut(s) 1229, 1261
PstI CTGCAG 2 cut(s) 745, 964
PstNI CAGNNNCTG 1 cut(s) 1928
PsuI RGATCY 1 cut(s) 663
PvuI CGATCG 1 cut(s) 1716
PvuII CAGCTG 3 cut(s) 913, 1441, 2720
RsaI GTAC 7 cut(s) 63, 557, 854, 1563, 1662, 2343, 2968
RsaNI GTAC 7 cut(s) 62, 556, 853, 1562, 1661, 2342, 2967
RseI CAYNNNNRTG 2 cut(s) 2352, 3029
SacI GAGCTC 1 cut(s) 428
SatI GCNGC 8 cut(s) 911, 1024, 1222, 1520, 1723, 2073, 2718, 3022
Sau96I GGNCC 3 cut(s) 1334, 1406, 1698
SbfI CCTGCAGG 1 cut(s) 964
SchI GAGTC 1 cut(s) 1790
ScrFI CCNGG 5 cut(s) 260, 453, 1226, 2446, 2517
SdaI CCTGCAGG 1 cut(s) 964
SduI GDGCHC 3 cut(s) 428, 2095, 3056
SfaNI GCATC 3 cut(s) 1350, 1506, 2515
SfcI CTRYAG 4 cut(s) 741, 960, 2574, 2833
Sfr274I CTCGAG 1 cut(s) 2474
SfuI TTCGAA 3 cut(s) 1292, 1607, 1620
SinI GGWCC 1 cut(s) 1698
SlaI CTCGAG 1 cut(s) 2474
SmiMI CAYNNNNRTG 2 cut(s) 2352, 3029
SmlI CTYRAG 6 cut(s) 833, 986, 1216, 2474, 2855, 3011
SmoI CTYRAG 6 cut(s) 833, 986, 1216, 2474, 2855, 3011
Sse8387I CCTGCAGG 1 cut(s) 964
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 7 cut(s) 570, 585, 1023, 1221, 1415, 2009, 2286
SspMI CTAG 6 cut(s) 632, 695, 977, 1379, 1461, 2270
SstI GAGCTC 1 cut(s) 428
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 258, 451, 1224, 2444, 2515
StyI CCWWGG 4 cut(s) 367, 387, 1369, 2269
TaiI ACGT 2 cut(s) 557, 2767
TaqII GACCGA 1 cut(s) 2893
TatI WGTACW 4 cut(s) 852, 1561, 1660, 2966
TauI GCSGC 2 cut(s) 1026, 1224
TfiI GAWTC 8 cut(s) 122, 547, 1165, 1882, 2141, 2419, 2735, 3064
TscAI CASTG 8 cut(s) 133, 766, 894, 1117, 1933, 2340, 2593, 2639
TseFI GTSAC 3 cut(s) 889, 1136, 1821
TseI GCWGC 6 cut(s) 910, 1519, 1722, 2072, 2717, 3021
Tsp45I GTSAC 3 cut(s) 889, 1136, 1821
TspDTI ATGAA 6 cut(s) 17, 67, 304, 557, 1062, 2252
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 8 cut(s) 133, 766, 894, 1117, 1933, 2340, 2593, 2639
Van91I CCANNNNNTGG 3 cut(s) 677, 1928, 2795
Vha464I CTTAAG 1 cut(s) 1216
VneI GTGCAC 1 cut(s) 3052
VpaK11BI GGWCC 1 cut(s) 1698
XapI RAATTY 9 cut(s) 149, 375, 882, 991, 1266, 1294, 1382, 1917, 2211
XbaI TCTAGA 1 cut(s) 1460
XceI RCATGY 6 cut(s) 240, 859, 1243, 1750, 2541, 2744
XcmI CCANNNNNNNNNTGG 2 cut(s) 22, 2946
XhoI CTCGAG 1 cut(s) 2474
XmaJI CCTAGG 1 cut(s) 2269
XmiI GTMKAC 1 cut(s) 1195
XspI CTAG 6 cut(s) 632, 695, 977, 1379, 1461, 2270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.