Rw3G004870
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013738
Physical Location & Seq
Reverse (-)
4099708 .. 4102916
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004870.1

Sequence Viewer

Length: 3048 bp
ATGGCTTACAATCTGAACTATTGTATTACAGAAATGGTACTATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAATGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCTTCTGCAACTGGACTGGAATTACATGCCACCAACGTCTCCAAAACCGGGTTGTAGGAATTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAAACTCTCTCTTCTTACCAACCTGTCTTTACAAAGCAATAGGTTCCATGGACAAATTCCATCTTCCTTGGGAATACTTAAAGATTTGGCATTTCTGAATTTGAGCTCAAACAAGCTTGAAGGTAGCATTCCAGGTTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACTTGACTTACAACAATCTATCTGGTGTTCTTCCTAAAGAACTCGGCTGGGTGAAAAATCTAACATACTTGGCTCTCTCCGTAAACAGTCTCAGCGGAGTGCTTCCAGCCTCACTTTTAAACTTGACGGAATTGACACAACTAGAACTGGCTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTCTGTATCTCCATACAAACTACCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTACGCGAAATCACTCTGATTGAGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAGAAACAAGCTTTCTGGGAGTATTCCAGTGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGCAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATTCCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTAACGGCTCTAACTAACTGTTCATATCTGCAAAAACTACATTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTGATAACCACATTACAGGAAGTATACCAGATAGTCTCGGAAACTTAAGCGGCCTGGTAACCTTGTACTTGTCATACAACCGTTTGGAAGGAAAAATTCCTTCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAGATGACTTAGGGATGATGTCTAACCTTGGTGTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCAATTGAGCTTACTCAATGTTCTCTTATCATGCTGCTTGATCTATCCTTCAACAACTTACAAGGCTCTGTTCCTGTACAGATTGACCGTTTTTCCAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAACTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATCTGTACTGGCAATTGACTTGTCTGACAACCAGTTTTCTGGGTCCATACCTACATTGATGGGAAGCTGCATATCTTTGGCGTATCTAAACATGTGCAACAACATATTTGAGGGGACTATTCCAGAGTCGTTGAAGTTGATCACTCAACTTGAAGTTTTGGACTTGTCACTTAATCGATTAAATGGCAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGGTTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCTGCACTACTTGGTCGTCCGCCATGCGTAGTTCAAAAGAGAGAATATATAATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCAGTTGCAGTAGTCTGTGTGCTGGTTGCTGTCTTCGTTTATTGTTTCTTCTTCTTCAGAAAACAGGATTCAAAACCAGAACACCGTGAACACGGAATGCTAAGAGTAATGGGATCCCCAAGCCATCGTGGAAGTCGAACTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGAGTTCAATGAAGCTCATCTCCTGGGTAGAGGGACCTTCGGAACAGTCTATAAAGCAATTGTTGATGACGGGGATACTATTATGGCAGTGAAGGTTCTACATGGTGACAGTATTCAGAGTTACAAAAGCTTTAAGAGGGAATGCCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTAAGGATGGTAGGATATACCTGGAACACACAGTTCAAGGCTCTTATTCTTGAATATGTAGGAAATGGTAACTTGGCAGAGCATCTCTATCCTGGTGGACTAGAGGAAGGAGCATGTGAATTAACATTGTGGGAAAGATTGTCTATAGCTATAGATGTTGCCAATGGCTTGGAGTATCTTCAAGAAGGTTGTCCAGTCCAAATTCTACATTGTGATTTAAAACCAGAGAACGTGCTTATTGACAATGATATGGTGGCTCACGTGGCAGATTTTGGGATTGGAAAGCTCATATCAGCTGACAAACCCAATGAATTGCATGTTAGCACAACGCATTTTCTACGAGGATCGATTGGTTACATTCCCCCAGAATATGGGCAAGGAAATGAGGTATCAACTAAAGGAGATATCTATAGCTTTGGTGTGATGGTGCTCGAGTTGATAACAAGAAAAAGACCAACAAGCAATATGTTTCCAGATGAGGTTGATCTAAGGAACTGGGTACATTCTTCGTACCCAGACCATGTTTTGGATGTCGTTGACAGTGCACTGAAGGAGATAAAAAGTACAGAGGGTGCTTTGCAAGAGCTTGAGAGATGCTGCATTCAAATGCTTGATGTAGGGTTGATGTGCACAGAAGATACTCCACAAGAAAGACCCTCCATGTCTTTTGTTGTGCAGAAACTGACACAGTGTCTCAAAAGCTCCAAGTTCATGTGA

Protein Analysis

1015

Amino Acids

112.11

Weight (kDa)

6.13

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 49 4.8e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 81 - 139 2e-07 Leucine rich repeat
LRR_14 PF23598 122 - 232 2.5e-08 Leucine-rich repeat region
LRR_14 PF23598 317 - 459 1.1e-12 Leucine-rich repeat region
LRR_8 PF13855 354 - 412 1e-08 Leucine rich repeat
LRR_4 PF12799 378 - 412 3.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 400 - 460 3e-07 Leucine rich repeat
Pkinase PF00069 721 - 1003 4.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 724 - 1005 1.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1806
Acc36I ACCTGC 1 cut(s) 655
AccB7I CCANNNNNTGG 4 cut(s) 584, 1835, 2702, 2857
AccI GTMKAC 1 cut(s) 1102
AccII CGCG 1 cut(s) 678
AciI CCGC 5 cut(s) 492, 930, 1128, 1322, 1916
AclWI GGATC 5 cut(s) 74, 565, 2088, 2101, 2683
AcsI RAATTY 8 cut(s) 282, 325, 898, 1173, 1201, 1289, 1824, 2532
AcuI CTGAAG 3 cut(s) 705, 2020, 2900
AcvI CACGTG 1 cut(s) 2593
AfaI GTAC 9 cut(s) 39, 761, 1145, 1284, 1470, 1569, 2832, 2843, 2896
AfiI CCNNNNNNNGG 6 cut(s) 166, 584, 1331, 1835, 2702, 2857
AflII CTTAAG 1 cut(s) 1123
AflIII ACRYGT 2 cut(s) 762, 1653
AhdI GACNNNNNGTC 1 cut(s) 3021
AjnI CCWGG 5 cut(s) 358, 1131, 2175, 2351, 2422
AjuI GAANNNNNNNTTGG 2 cut(s) 1309, 1341
Alw21I GWGCWC 4 cut(s) 335, 2763, 2878, 2963
Alw26I GTCTC 4 cut(s) 161, 491, 1118, 3029
Alw44I GTGCAC 2 cut(s) 2874, 2959
AlwI GGATC 5 cut(s) 74, 565, 2088, 2101, 2683
AlwNI CAGNNNCTG 2 cut(s) 1835, 3013
Ama87I CYCGRG 1 cut(s) 2762
AoxI GGCC 3 cut(s) 1129, 1241, 1313
ApaLI GTGCAC 2 cut(s) 2874, 2959
ApeKI GCWGC 4 cut(s) 1426, 1629, 1979, 2928
ApoI RAATTY 8 cut(s) 282, 325, 898, 1173, 1201, 1289, 1824, 2532
AspS9I GGNCC 4 cut(s) 1241, 1313, 1605, 2187
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 6 cut(s) 207, 460, 1055, 2116, 2165, 2270
AsuII TTCGAA 3 cut(s) 1199, 1514, 1527
AvaI CYCGRG 1 cut(s) 2762
AvaII GGWCC 2 cut(s) 1605, 2187
BaeGI GKGCMC 2 cut(s) 2878, 2963
BamHI GGATCC 1 cut(s) 2093
BanII GRGCYC 1 cut(s) 335
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbrPI CACGTG 1 cut(s) 2593
BbsI GAAGAC 1 cut(s) 2005
Bbv12I GWGCWC 4 cut(s) 335, 2763, 2878, 2963
BbvI GCAGC 4 cut(s) 1413, 1616, 1991, 2915
BccI CCATC 7 cut(s) 79, 295, 1039, 1615, 2112, 2332, 2749
BceAI ACGGC 1 cut(s) 978
BciT130I CCWGG 5 cut(s) 360, 1133, 2177, 2353, 2424
BciVI GTATCC 1 cut(s) 2221
BclI TGATCA 2 cut(s) 1701, 1782
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 4 cut(s) 161, 491, 1118, 3029
BfaI CTAG 6 cut(s) 539, 602, 884, 1286, 1368, 2432
BfmI CTRYAG 5 cut(s) 648, 867, 2475, 2481, 2740
BfrI CTTAAG 1 cut(s) 1123
BfuAI ACCTGC 1 cut(s) 655
BfuI GTATCC 1 cut(s) 2221
BisI GCNGC 6 cut(s) 931, 1129, 1427, 1630, 1980, 2929
BlsI GCNGC 6 cut(s) 932, 1130, 1428, 1631, 1981, 2930
Bme1390I CCNGG 6 cut(s) 167, 360, 1133, 2177, 2353, 2424
Bme18I GGWCC 2 cut(s) 1605, 2187
BmeRI GACNNNNNGTC 1 cut(s) 3021
BmeT110I CYCGRG 1 cut(s) 2762
BmgT120I GGNCC 4 cut(s) 1241, 1313, 1605, 2187
BmiI GGNNCC 4 cut(s) 272, 1606, 2095, 2188
BmrFI CCNGG 6 cut(s) 167, 360, 1133, 2177, 2353, 2424
BmrI ACTGGG 2 cut(s) 1845, 2836
BmsI GCATC 2 cut(s) 2422, 2915
BmuI ACTGGG 2 cut(s) 1845, 2836
BpiI GAAGAC 1 cut(s) 2005
Bpu14I TTCGAA 3 cut(s) 1199, 1514, 1527
BpuEI CTTGAG 3 cut(s) 725, 914, 2939
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 3 cut(s) 1738, 1958, 2678
BsaAI YACGTR 1 cut(s) 2593
BsaJI CCNNGG 4 cut(s) 274, 294, 1276, 2176
BsaWI WCCGGW 1 cut(s) 2322
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 584, 1331, 1835, 2702, 2857
BseBI CCWGG 5 cut(s) 360, 1133, 2177, 2353, 2424
BseCI ATCGAT 3 cut(s) 1738, 1958, 2678
BseDI CCNNGG 4 cut(s) 274, 294, 1276, 2176
BseGI GGATG 3 cut(s) 1269, 2343, 2866
BseLI CCNNNNNNNGG 6 cut(s) 166, 584, 1331, 1835, 2702, 2857
BseMII CTCAG 3 cut(s) 502, 964, 1340
BseSI GKGCMC 2 cut(s) 2878, 2963
BseXI GCAGC 4 cut(s) 1413, 1616, 1991, 2915
BseYI CCCAGC 1 cut(s) 444
BsgI GTGCAG 3 cut(s) 649, 1884, 3026
Bsh1236I CGCG 1 cut(s) 678
BshFI GGCC 3 cut(s) 1131, 1243, 1315
BshVI ATCGAT 3 cut(s) 1738, 1958, 2678
BsiHKAI GWGCWC 4 cut(s) 335, 2763, 2878, 2963
BsiHKCI CYCGRG 1 cut(s) 2762
BsiSI CCGG 2 cut(s) 166, 2323
BslFI GGGAC 2 cut(s) 1690, 2200
BslI CCNNNNNNNGG 6 cut(s) 166, 584, 1331, 1835, 2702, 2857
BsmAI GTCTC 4 cut(s) 161, 491, 1118, 3029
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 1690, 2200
BsmI GAATGC 4 cut(s) 354, 2082, 2300, 2931
BsnI GGCC 3 cut(s) 1131, 1243, 1315
BsoBI CYCGRG 1 cut(s) 2762
Bsp119I TTCGAA 3 cut(s) 1199, 1514, 1527
Bsp1286I GDGCHC 4 cut(s) 335, 2763, 2878, 2963
Bsp1407I TGTACA 2 cut(s) 759, 1468
Bsp143I GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 5 cut(s) 492, 930, 1128, 1322, 1916
BspANI GGCC 3 cut(s) 1131, 1243, 1315
BspCNI CTCAG 3 cut(s) 501, 963, 1341
BspDI ATCGAT 3 cut(s) 1738, 1958, 2678
BspFNI CGCG 1 cut(s) 678
BspLI GGNNCC 4 cut(s) 272, 1606, 2095, 2188
BspMAI CTGCAG 2 cut(s) 652, 871
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 5 cut(s) 74, 565, 2088, 2101, 2683
BspT104I TTCGAA 3 cut(s) 1199, 1514, 1527
BspTI CTTAAG 1 cut(s) 1123
BsrGI TGTACA 2 cut(s) 759, 1468
BssECI CCNNGG 4 cut(s) 274, 294, 1276, 2176
BssMI GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
BssNAI GTATAC 1 cut(s) 1103
BssT1I CCWWGG 3 cut(s) 274, 294, 1276
Bst1107I GTATAC 1 cut(s) 1103
Bst2UI CCWGG 5 cut(s) 360, 1133, 2177, 2353, 2424
Bst6I CTCTTC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 1123
BstAPI GCANNNNNTGC 1 cut(s) 1985
BstAUI TGTACA 2 cut(s) 759, 1468
BstBAI YACGTR 1 cut(s) 2593
BstBI TTCGAA 3 cut(s) 1199, 1514, 1527
BstC8I GCNNGC 5 cut(s) 200, 881, 1193, 1542, 1546
BstDEI CTNAG 6 cut(s) 488, 950, 1258, 1349, 2081, 2819
BstDSI CCRYGG 1 cut(s) 274
BstEII GGTNACC 2 cut(s) 1043, 1135
BstF5I GGATG 3 cut(s) 1269, 2343, 2866
BstFNI CGCG 1 cut(s) 678
BstKTI GATC 8 cut(s) 82, 573, 1435, 1704, 1785, 2096, 2678, 2818
BstMAI GTCTC 4 cut(s) 161, 491, 1118, 3029
BstMBI GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
BstMWI GCNNNNNNNGC 3 cut(s) 1321, 1985, 2594
BstNI CCWGG 5 cut(s) 360, 1133, 2177, 2353, 2424
BstNSI RCATGY 5 cut(s) 147, 766, 1657, 2448, 2651
BstPI GGTNACC 2 cut(s) 1043, 1135
BstSCI CCNGG 6 cut(s) 165, 358, 1131, 2175, 2351, 2422
BstSFI CTRYAG 5 cut(s) 648, 867, 2475, 2481, 2740
BstSLI GKGCMC 2 cut(s) 2878, 2963
BstUI CGCG 1 cut(s) 678
BstV1I GCAGC 4 cut(s) 1413, 1616, 1991, 2915
BstV2I GAAGAC 1 cut(s) 2005
BstX2I RGATCY 2 cut(s) 570, 2093
BstXI CCANNNNNNTGG 3 cut(s) 922, 1601, 2500
BstYI RGATCY 2 cut(s) 570, 2093
BstZ17I GTATAC 1 cut(s) 1103
Bsu15I ATCGAT 3 cut(s) 1738, 1958, 2678
BsuI GTATCC 1 cut(s) 2221
BsuRI GGCC 3 cut(s) 1131, 1243, 1315
BsuTUI ATCGAT 3 cut(s) 1738, 1958, 2678
BtgI CCRYGG 1 cut(s) 274
BtsCI GGATG 3 cut(s) 1269, 2343, 2866
BtsI GCAGTG 2 cut(s) 666, 2247
BtsIMutI CAGTG 8 cut(s) 666, 801, 1017, 1833, 2247, 2876, 2878, 3026
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 5 cut(s) 200, 881, 1193, 1542, 1546
CaiI CAGNNNCTG 2 cut(s) 1835, 3013
Cfr13I GGNCC 4 cut(s) 1241, 1313, 1605, 2187
ClaI ATCGAT 3 cut(s) 1738, 1958, 2678
Csp6I GTAC 9 cut(s) 38, 760, 1144, 1283, 1469, 1568, 2831, 2842, 2895
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviQI GTAC 9 cut(s) 38, 760, 1144, 1283, 1469, 1568, 2831, 2842, 2895
DdeI CTNAG 6 cut(s) 488, 950, 1258, 1349, 2081, 2819
DpnI GATC 8 cut(s) 81, 572, 1434, 1703, 1784, 2095, 2677, 2817
DpnII GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
DraI TTTAAA 2 cut(s) 516, 2550
DriI GACNNNNNGTC 1 cut(s) 3021
Eam1104I CTCTTC 1 cut(s) 243
Eam1105I GACNNNNNGTC 1 cut(s) 3021
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 2 cut(s) 1311, 1905
Ecl136II GAGCTC 1 cut(s) 333
Eco130I CCWWGG 3 cut(s) 274, 294, 1276
Eco24I GRGCYC 1 cut(s) 335
Eco32I GATATC 3 cut(s) 213, 1355, 2737
Eco47I GGWCC 2 cut(s) 1605, 2187
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 3 cut(s) 705, 2020, 2900
Eco72I CACGTG 1 cut(s) 2593
Eco88I CYCGRG 1 cut(s) 2762
Eco91I GGTNACC 2 cut(s) 1043, 1135
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 2187
EcoO65I GGTNACC 2 cut(s) 1043, 1135
EcoRII CCWGG 5 cut(s) 358, 1131, 2175, 2351, 2422
EcoRV GATATC 3 cut(s) 213, 1355, 2737
EcoT14I CCWWGG 3 cut(s) 274, 294, 1276
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 3 cut(s) 274, 294, 1276
Esp3I CGTCTC 1 cut(s) 161
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 2104, 2136
FaqI GGGAC 2 cut(s) 1690, 2200
FbaI TGATCA 2 cut(s) 1701, 1782
FblI GTMKAC 1 cut(s) 1102
Fnu4HI GCNGC 6 cut(s) 931, 1129, 1427, 1630, 1980, 2929
FokI GGATG 3 cut(s) 1276, 2350, 2873
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 6 cut(s) 931, 1129, 1427, 1630, 1980, 2929
FspBI CTAG 6 cut(s) 539, 602, 884, 1286, 1368, 2432
GluI GCNGC 6 cut(s) 931, 1129, 1427, 1630, 1980, 2929
GsaI CCCAGC 1 cut(s) 448
HaeIII GGCC 3 cut(s) 1131, 1243, 1315
HapII CCGG 2 cut(s) 166, 2323
HincII GTYRAC 2 cut(s) 553, 2869
HindII GTYRAC 2 cut(s) 553, 2869
HindIII AAGCTT 5 cut(s) 341, 382, 776, 1193, 2281
HinfI GANTC 6 cut(s) 607, 1072, 1688, 1789, 2048, 2326
HpaII CCGG 2 cut(s) 166, 2323
HphI GGTGA 6 cut(s) 207, 460, 1055, 2116, 2165, 2270
HpyCH4IV ACGT 3 cut(s) 154, 2562, 2592
HpyF10VI GCNNNNNNNGC 3 cut(s) 1321, 1985, 2594
HpyF3I CTNAG 6 cut(s) 488, 950, 1258, 1349, 2081, 2819
HpySE526I ACGT 3 cut(s) 154, 2562, 2592
Ksp22I TGATCA 2 cut(s) 1701, 1782
Kzo9I GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
LmnI GCTCC 3 cut(s) 587, 2441, 3038
Lsp1109I GCAGC 4 cut(s) 1413, 1616, 1991, 2915
LweI GCATC 2 cut(s) 2422, 2915
MaeI CTAG 6 cut(s) 539, 602, 884, 1286, 1368, 2432
MaeII ACGT 3 cut(s) 154, 2562, 2592
MaeIII GTNAC 9 cut(s) 659, 796, 1043, 1135, 1728, 2258, 2273, 2399, 2684
MalI GATC 8 cut(s) 81, 572, 1434, 1703, 1784, 2095, 2677, 2817
MboI GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
MfeI CAATTG 3 cut(s) 1395, 1575, 2211
MflI RGATCY 2 cut(s) 570, 2093
MhlI GDGCHC 4 cut(s) 335, 2763, 2878, 2963
MlyI GAGTC 1 cut(s) 1697
MmeI TCCRAC 1 cut(s) 939
MslI CAYNNNNRTG 1 cut(s) 2936
MspA1I CMGCKG 4 cut(s) 492, 820, 1348, 2627
MspCI CTTAAG 1 cut(s) 1123
MspI CCGG 2 cut(s) 166, 2323
MspR9I CCNGG 6 cut(s) 167, 360, 1133, 2177, 2353, 2424
MunI CAATTG 3 cut(s) 1395, 1575, 2211
Mva1269I GAATGC 4 cut(s) 354, 2082, 2300, 2931
MvaI CCWGG 5 cut(s) 360, 1133, 2177, 2353, 2424
MvnI CGCG 1 cut(s) 678
MwoI GCNNNNNNNGC 3 cut(s) 1321, 1985, 2594
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 1 cut(s) 274
NdeII GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
NlaIV GGNNCC 4 cut(s) 272, 1606, 2095, 2188
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 4 cut(s) 796, 1043, 1728, 2258
NspI RCATGY 5 cut(s) 147, 766, 1657, 2448, 2651
NspV TTCGAA 3 cut(s) 1199, 1514, 1527
PaeR7I CTCGAG 1 cut(s) 2762
PciI ACATGT 2 cut(s) 762, 1653
PcsI WCGNNNNNNNCGW 1 cut(s) 2113
PctI GAATGC 4 cut(s) 354, 2082, 2300, 2931
PfeI GAWTC 5 cut(s) 607, 1072, 1789, 2048, 2326
PflMI CCANNNNNTGG 4 cut(s) 584, 1835, 2702, 2857
PkrI GCNGC 6 cut(s) 932, 1130, 1428, 1631, 1981, 2930
PleI GAGTC 1 cut(s) 1696
PmaCI CACGTG 1 cut(s) 2593
PmlI CACGTG 1 cut(s) 2593
PpsI GAGTC 1 cut(s) 1696
Ppu21I YACGTR 1 cut(s) 2593
PpuMI RGGWCCY 1 cut(s) 2187
PscI ACATGT 2 cut(s) 762, 1653
PsiI TTATAA 1 cut(s) 1806
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 2187
Psp6I CCWGG 5 cut(s) 358, 1131, 2175, 2351, 2422
PspCI CACGTG 1 cut(s) 2593
PspEI GGTNACC 2 cut(s) 1043, 1135
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 5 cut(s) 358, 1131, 2175, 2351, 2422
PspN4I GGNNCC 4 cut(s) 272, 1606, 2095, 2188
PspPI GGNCC 4 cut(s) 1241, 1313, 1605, 2187
PspPPI RGGWCCY 1 cut(s) 2187
PspXI VCTCGAGB 1 cut(s) 2762
PstI CTGCAG 2 cut(s) 652, 871
PstNI CAGNNNCTG 2 cut(s) 1835, 3013
PsuI RGATCY 2 cut(s) 570, 2093
PvuII CAGCTG 3 cut(s) 820, 1348, 2627
RsaI GTAC 9 cut(s) 39, 761, 1145, 1284, 1470, 1569, 2832, 2843, 2896
RsaNI GTAC 9 cut(s) 38, 760, 1144, 1283, 1469, 1568, 2831, 2842, 2895
RseI CAYNNNNRTG 1 cut(s) 2936
SacI GAGCTC 1 cut(s) 335
SatI GCNGC 6 cut(s) 931, 1129, 1427, 1630, 1980, 2929
Sau3AI GATC 8 cut(s) 79, 570, 1432, 1701, 1782, 2093, 2675, 2815
Sau96I GGNCC 4 cut(s) 1241, 1313, 1605, 2187
SbfI CCTGCAGG 1 cut(s) 871
SchI GAGTC 1 cut(s) 1697
ScrFI CCNGG 6 cut(s) 167, 360, 1133, 2177, 2353, 2424
SdaI CCTGCAGG 1 cut(s) 871
SduI GDGCHC 4 cut(s) 335, 2763, 2878, 2963
SfaNI GCATC 2 cut(s) 2422, 2915
SfcI CTRYAG 5 cut(s) 648, 867, 2475, 2481, 2740
Sfr274I CTCGAG 1 cut(s) 2762
SfuI TTCGAA 3 cut(s) 1199, 1514, 1527
SinI GGWCC 2 cut(s) 1605, 2187
SlaI CTCGAG 1 cut(s) 2762
SmiMI CAYNNNNRTG 1 cut(s) 2936
SmlI CTYRAG 5 cut(s) 740, 893, 1123, 2762, 2918
SmoI CTYRAG 5 cut(s) 740, 893, 1123, 2762, 2918
Sse8387I CCTGCAGG 1 cut(s) 871
SsiI CCGC 5 cut(s) 492, 930, 1128, 1322, 1916
SspMI CTAG 6 cut(s) 539, 602, 884, 1286, 1368, 2432
SstI GAGCTC 1 cut(s) 335
StyD4I CCNGG 6 cut(s) 165, 358, 1131, 2175, 2351, 2422
StyI CCWWGG 3 cut(s) 274, 294, 1276
TaiI ACGT 3 cut(s) 157, 2565, 2595
TatI WGTACW 6 cut(s) 759, 1143, 1282, 1468, 1567, 2894
TauI GCSGC 2 cut(s) 933, 1131
TfiI GAWTC 5 cut(s) 607, 1072, 1789, 2048, 2326
TscAI CASTG 8 cut(s) 673, 801, 1024, 1840, 2247, 2878, 2883, 3026
TseFI GTSAC 4 cut(s) 796, 1043, 1728, 2258
TseI GCWGC 4 cut(s) 1426, 1629, 1979, 2928
Tsp45I GTSAC 4 cut(s) 796, 1043, 1728, 2258
TspDTI ATGAA 7 cut(s) 43, 211, 354, 969, 2178, 2655, 3031
TspGWI ACGGA 3 cut(s) 466, 539, 2088
TspRI CASTG 8 cut(s) 673, 801, 1024, 1840, 2247, 2878, 2883, 3026
Van91I CCANNNNNTGG 4 cut(s) 584, 1835, 2702, 2857
Vha464I CTTAAG 1 cut(s) 1123
VneI GTGCAC 2 cut(s) 2874, 2959
VpaK11BI GGWCC 2 cut(s) 1605, 2187
XapI RAATTY 8 cut(s) 282, 325, 898, 1173, 1201, 1289, 1824, 2532
XbaI TCTAGA 1 cut(s) 1367
XceI RCATGY 5 cut(s) 147, 766, 1657, 2448, 2651
XhoI CTCGAG 1 cut(s) 2762
XmiI GTMKAC 1 cut(s) 1102
XspI CTAG 6 cut(s) 539, 602, 884, 1286, 1368, 2432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.