Rh3BG064400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013738
Physical Location & Seq
Reverse (-)
4318404 .. 4321612
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG064400.1

Sequence Viewer

Length: 3117 bp
ATGGCTTACAATCTGAACTATTGTATTACAGAAATGGTACTATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAAGTCTGCATTTCTTTGTGAAGATTCCAGTGATTGCAACACTCTGCTCAAATTCAAGGAGGAGCTAACAAGTGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCTTCTGCAACTGGACTGGAATTACATGCCACCAACGTCTCCAAAACCGGGTTATAGGAATTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAAACTCTCTTTTCTTACCAACCTGTCTTTACAAAGCAATAGGTTCCATGGACAAATTCCATCTTCCTTGGGAATACTTAAAGATTTGGCATTTCTGAATTTGAGCTCAAACAAGCTTGAAGGTAGCATTCCAGGTTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACTTGACTTACAACAATCTATCTGGTGTTCTTCCTAAAGAACTCGGCTGGGTGAAGAATCTAACATACTTGGCTCTCTCCGTAAACAGTCTCAGTGGAGTGCTTCCAGCCTCACTTTTAAACTTGACGGAATTGACACAACTAGAACTGGCTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTCTGTATCTCCATACAAACTACCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTACGCGAAATCACTCTGATTGAGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAGAAACAAGCTTTCTGGGAGTATTCCAGTGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGCAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATTCCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTAACGGCTCTAACTAACTGTTCATATCTGCAAAAACTACATTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTTATAACCACATTACAGGAAGTATACCAGATAGTCTCGGAAACTTAAGCGGCCTGGTAACCTTGTACTTGTCATACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCAATGCCAAAAGACTTGGGGATGATGCCTAACCTTGGTTTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCGATTGAGCTTACTCAATGTTCTCTTATGCTGCTGCTTGATCTATCTTTCAACAACTTAAAAGGCTCTGTTCCTGTACAGATTGACCGTTTTTCAAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAACTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATTTGTACAGGCAATTGACTTGTCTGGCAACCAGTTTTTTGGGTCCATACCTACATTGATGGGAAGCTGCATATCTTTGGCGTATCTAAACATGTCCAACAACATATTTGAGGGGACTATTCCAGAGTCGTTGAAGTTGATCACTCAACTTGAAGTTTTGGACTTGTCACTTAATCGATTAAATGGTAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGAATCTCAATTTATCTTATAACAGGTTATCAGGAGAAGTTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCTGCACTACTTGGTCGTCCGCCATGCGTAGTTCAAAAGAGAAAATATATAATAAGAAAATCGATACTTTACTCTTTGGTTGCAGCGGTTGCATTAGTCTGTGTGCTGGTTGCTGTCTTCGTTTATTGTTTCTTCTTCTTCAGAAAACGGGATTCAAAACCAGAACACCGTGAACACAGAATGCTAAGAGTAATGGGATCCCCAAGCCATCGTGGAAGTCGAACTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGAGTTCAATGAAGCTCATCTCCTGGGTAGAGGGACCTTCGGAACAGTCTATAAAGCAATTGTTGATGACGGGGATACTATTATGGCAGTGAAGGTTCTACATGGTGATAGTATTCAGAGTTACAAAAGCTTTAAGAGGGAATGCCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTGAGGATGGTAGGATATACCTGGAACACACAGTTCAAGGCTCTTATTCTTGAATATGTAGGAAATGGTAACTTGGCAGAGCATCTCTATCCTGGTGGACTAGAGGAAGGAGCATGTGAATTAACATTGTGGGAAAGATTGTCTATAGCTATAGATGTTGCCAATGGCTTGGAGTATCTTCAAGAAGGTTGTCCAGTCCAAATTCTACATTGTGATTTAAAACCAGAGAACGTGCTTATTGACAATGATATGGTGGCTCACGTGGCAGATTTTGGGATTGGAAAGCTCATATCAGCTGACAAACCCAATGAATTGCATGTTAGCACAACGCATTTTCTACGAGGATCGATTGGTTACATTCCCCCAGAATATGGGCAAGGAAATGAGGTATCAACTAAAGGAGATATCTATAGCTTTGGTGTGATGGTGCTCGAGTTGATAACAAGAAAAAGACCAACAAGCAATATGTTTCCAGATGAGGTTGATCTAAGGAACTGGGTACATTCTTCGTACCCAGACCATGTTTTGGATGTCGTTGACAGTGCACTGAAGGAGATAAAAAGTACAGAGGGTGCTTTGCAAGAGCTTGAGAGATGCTGCATTCAAATGCTTGATGTAGGGTTGATGTGCACAGAAGATACTCCACAAGAAAGACCCTCCATGTCTTTTGTTGTGCAGAAACTGACACAGTGTCTCAAAAGCTCCAAGTTCATGTGA

Protein Analysis

1038

Amino Acids

115.01

Weight (kDa)

6.73

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 8.1e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 104 - 162 3.9e-07 Leucine rich repeat
LRR_14 PF23598 145 - 255 2.9e-08 Leucine-rich repeat region
LRR_14 PF23598 340 - 477 7.2e-11 Leucine-rich repeat region
LRR_8 PF13855 377 - 435 1.2e-08 Leucine rich repeat
LRR_4 PF12799 401 - 435 3.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 423 - 483 2.2e-06 Leucine rich repeat
Pkinase PF00069 744 - 1026 4.8e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 747 - 1028 1.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 1875
Acc36I ACCTGC 1 cut(s) 724
AccB7I CCANNNNNTGG 4 cut(s) 653, 1904, 2771, 2926
AccI GTMKAC 1 cut(s) 1171
AccII CGCG 1 cut(s) 747
AciI CCGC 5 cut(s) 999, 1197, 1391, 1985, 2051
AclWI GGATC 5 cut(s) 143, 634, 2157, 2170, 2752
AcsI RAATTY 9 cut(s) 125, 351, 394, 967, 1242, 1270, 1358, 1558, 2601
AcuI CTGAAG 3 cut(s) 774, 2089, 2969
AcvI CACGTG 1 cut(s) 2662
AfaI GTAC 8 cut(s) 39, 830, 1214, 1539, 1638, 2901, 2912, 2965
AfiI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
AflII CTTAAG 1 cut(s) 1192
AflIII ACRYGT 2 cut(s) 831, 1722
AhdI GACNNNNNGTC 1 cut(s) 3090
AjnI CCWGG 5 cut(s) 427, 1200, 2244, 2420, 2491
AjuI GAANNNNNNNTTGG 2 cut(s) 1378, 1410
Alw21I GWGCWC 4 cut(s) 404, 2832, 2947, 3032
Alw26I GTCTC 4 cut(s) 230, 560, 1187, 3098
Alw44I GTGCAC 2 cut(s) 2943, 3028
AlwI GGATC 5 cut(s) 143, 634, 2157, 2170, 2752
AlwNI CAGNNNCTG 2 cut(s) 1904, 3082
Ama87I CYCGRG 1 cut(s) 2831
AoxI GGCC 3 cut(s) 1198, 1310, 1382
ApaLI GTGCAC 2 cut(s) 2943, 3028
ApeKI GCWGC 5 cut(s) 1492, 1495, 1698, 2048, 2997
ApoI RAATTY 9 cut(s) 125, 351, 394, 967, 1242, 1270, 1358, 1558, 2601
AspS9I GGNCC 4 cut(s) 1310, 1382, 1674, 2256
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 6 cut(s) 276, 529, 1124, 2185, 2234, 2339
AsuII TTCGAA 3 cut(s) 1268, 1583, 1596
AvaI CYCGRG 1 cut(s) 2831
AvaII GGWCC 2 cut(s) 1674, 2256
BaeGI GKGCMC 2 cut(s) 2947, 3032
BamHI GGATCC 1 cut(s) 2162
BanII GRGCYC 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 696, 728
BbrPI CACGTG 1 cut(s) 2662
BbsI GAAGAC 1 cut(s) 2074
Bbv12I GWGCWC 4 cut(s) 404, 2832, 2947, 3032
BbvI GCAGC 5 cut(s) 1479, 1482, 1685, 2060, 2984
BccI CCATC 8 cut(s) 79, 364, 1108, 1255, 1684, 2181, 2401, 2818
BceAI ACGGC 1 cut(s) 1047
BciT130I CCWGG 5 cut(s) 429, 1202, 2246, 2422, 2493
BciVI GTATCC 1 cut(s) 2290
BclI TGATCA 2 cut(s) 1770, 1851
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 4 cut(s) 230, 560, 1187, 3098
BfaI CTAG 6 cut(s) 608, 671, 953, 1355, 1437, 2501
BfmI CTRYAG 5 cut(s) 717, 936, 2544, 2550, 2809
BfrI CTTAAG 1 cut(s) 1192
BfuAI ACCTGC 1 cut(s) 724
BfuI GTATCC 1 cut(s) 2290
BisI GCNGC 7 cut(s) 1000, 1198, 1493, 1496, 1699, 2049, 2998
BlsI GCNGC 7 cut(s) 1001, 1199, 1494, 1497, 1700, 2050, 2999
Bme1390I CCNGG 6 cut(s) 236, 429, 1202, 2246, 2422, 2493
Bme18I GGWCC 2 cut(s) 1674, 2256
BmeRI GACNNNNNGTC 1 cut(s) 3090
BmeT110I CYCGRG 1 cut(s) 2831
BmgT120I GGNCC 4 cut(s) 1310, 1382, 1674, 2256
BmiI GGNNCC 4 cut(s) 341, 1675, 2164, 2257
BmrFI CCNGG 6 cut(s) 236, 429, 1202, 2246, 2422, 2493
BmrI ACTGGG 2 cut(s) 1914, 2905
BmsI GCATC 3 cut(s) 1326, 2491, 2984
BmuI ACTGGG 2 cut(s) 1914, 2905
BpiI GAAGAC 1 cut(s) 2074
Bpu14I TTCGAA 3 cut(s) 1268, 1583, 1596
BpuEI CTTGAG 3 cut(s) 794, 983, 3008
BpuMI CCSGG 1 cut(s) 236
Bsa29I ATCGAT 3 cut(s) 1807, 2027, 2747
BsaAI YACGTR 1 cut(s) 2662
BsaJI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BsaWI WCCGGW 1 cut(s) 2391
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
BseBI CCWGG 5 cut(s) 429, 1202, 2246, 2422, 2493
BseCI ATCGAT 3 cut(s) 1807, 2027, 2747
BseDI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BseGI GGATG 3 cut(s) 1338, 2412, 2935
BseLI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
BseMII CTCAG 3 cut(s) 571, 1033, 1409
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 2 cut(s) 2947, 3032
BseXI GCAGC 5 cut(s) 1479, 1482, 1685, 2060, 2984
BseYI CCCAGC 1 cut(s) 513
BsgI GTGCAG 3 cut(s) 718, 1953, 3095
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 3 cut(s) 1200, 1312, 1384
BshVI ATCGAT 3 cut(s) 1807, 2027, 2747
BsiHKAI GWGCWC 4 cut(s) 404, 2832, 2947, 3032
BsiHKCI CYCGRG 1 cut(s) 2831
BsiSI CCGG 2 cut(s) 235, 2392
BslFI GGGAC 2 cut(s) 1759, 2269
BslI CCNNNNNNNGG 7 cut(s) 235, 653, 1346, 1400, 1904, 2771, 2926
BsmAI GTCTC 4 cut(s) 230, 560, 1187, 3098
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 1759, 2269
BsmI GAATGC 4 cut(s) 423, 2151, 2369, 3000
BsnI GGCC 3 cut(s) 1200, 1312, 1384
BsoBI CYCGRG 1 cut(s) 2831
Bsp119I TTCGAA 3 cut(s) 1268, 1583, 1596
Bsp1286I GDGCHC 4 cut(s) 404, 2832, 2947, 3032
Bsp1407I TGTACA 3 cut(s) 828, 1537, 1636
Bsp143I GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 5 cut(s) 999, 1197, 1391, 1985, 2051
BspANI GGCC 3 cut(s) 1200, 1312, 1384
BspCNI CTCAG 3 cut(s) 570, 1032, 1410
BspDI ATCGAT 3 cut(s) 1807, 2027, 2747
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 4 cut(s) 341, 1675, 2164, 2257
BspMAI CTGCAG 2 cut(s) 721, 940
BspMI ACCTGC 1 cut(s) 724
BspPI GGATC 5 cut(s) 143, 634, 2157, 2170, 2752
BspT104I TTCGAA 3 cut(s) 1268, 1583, 1596
BspTI CTTAAG 1 cut(s) 1192
BsrGI TGTACA 3 cut(s) 828, 1537, 1636
BssECI CCNNGG 4 cut(s) 343, 363, 1345, 2245
BssMI GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
BssNAI GTATAC 1 cut(s) 1172
BssT1I CCWWGG 3 cut(s) 343, 363, 1345
Bst1107I GTATAC 1 cut(s) 1172
Bst2UI CCWGG 5 cut(s) 429, 1202, 2246, 2422, 2493
BstAFI CTTAAG 1 cut(s) 1192
BstAPI GCANNNNNTGC 1 cut(s) 2054
BstAUI TGTACA 3 cut(s) 828, 1537, 1636
BstBAI YACGTR 1 cut(s) 2662
BstBI TTCGAA 3 cut(s) 1268, 1583, 1596
BstC8I GCNNGC 5 cut(s) 269, 950, 1262, 1611, 1615
BstDEI CTNAG 6 cut(s) 75, 557, 1019, 1418, 2150, 2888
BstDSI CCRYGG 1 cut(s) 343
BstEII GGTNACC 2 cut(s) 1112, 1204
BstF5I GGATG 3 cut(s) 1338, 2412, 2935
BstFNI CGCG 1 cut(s) 747
BstKTI GATC 8 cut(s) 151, 642, 1504, 1773, 1854, 2165, 2747, 2887
BstMAI GTCTC 4 cut(s) 230, 560, 1187, 3098
BstMBI GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
BstMWI GCNNNNNNNGC 3 cut(s) 1390, 2054, 2663
BstNI CCWGG 5 cut(s) 429, 1202, 2246, 2422, 2493
BstNSI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
BstPI GGTNACC 2 cut(s) 1112, 1204
BstSCI CCNGG 6 cut(s) 234, 427, 1200, 2244, 2420, 2491
BstSFI CTRYAG 5 cut(s) 717, 936, 2544, 2550, 2809
BstSLI GKGCMC 2 cut(s) 2947, 3032
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 5 cut(s) 1479, 1482, 1685, 2060, 2984
BstV2I GAAGAC 1 cut(s) 2074
BstX2I RGATCY 2 cut(s) 639, 2162
BstXI CCANNNNNNTGG 3 cut(s) 991, 1670, 2569
BstYI RGATCY 2 cut(s) 639, 2162
BstZ17I GTATAC 1 cut(s) 1172
Bsu15I ATCGAT 3 cut(s) 1807, 2027, 2747
BsuI GTATCC 1 cut(s) 2290
BsuRI GGCC 3 cut(s) 1200, 1312, 1384
BsuTUI ATCGAT 3 cut(s) 1807, 2027, 2747
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 3 cut(s) 1338, 2412, 2935
BtsI GCAGTG 2 cut(s) 735, 2316
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 5 cut(s) 269, 950, 1262, 1611, 1615
CaiI CAGNNNCTG 2 cut(s) 1904, 3082
Cfr13I GGNCC 4 cut(s) 1310, 1382, 1674, 2256
ClaI ATCGAT 3 cut(s) 1807, 2027, 2747
Csp6I GTAC 8 cut(s) 38, 829, 1213, 1538, 1637, 2900, 2911, 2964
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 8 cut(s) 38, 829, 1213, 1538, 1637, 2900, 2911, 2964
DdeI CTNAG 6 cut(s) 75, 557, 1019, 1418, 2150, 2888
DpnI GATC 8 cut(s) 150, 641, 1503, 1772, 1853, 2164, 2746, 2886
DpnII GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
DraI TTTAAA 2 cut(s) 585, 2619
DriI GACNNNNNGTC 1 cut(s) 3090
Eam1105I GACNNNNNGTC 1 cut(s) 3090
EciI GGCGGA 2 cut(s) 1380, 1974
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 3 cut(s) 343, 363, 1345
Eco24I GRGCYC 1 cut(s) 404
Eco32I GATATC 3 cut(s) 282, 1424, 2806
Eco47I GGWCC 2 cut(s) 1674, 2256
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 3 cut(s) 774, 2089, 2969
Eco72I CACGTG 1 cut(s) 2662
Eco88I CYCGRG 1 cut(s) 2831
Eco91I GGTNACC 2 cut(s) 1112, 1204
EcoICRI GAGCTC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 2256
EcoO65I GGTNACC 2 cut(s) 1112, 1204
EcoRII CCWGG 5 cut(s) 427, 1200, 2244, 2420, 2491
EcoRV GATATC 3 cut(s) 282, 1424, 2806
EcoT14I CCWWGG 3 cut(s) 343, 363, 1345
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 3 cut(s) 343, 363, 1345
Esp3I CGTCTC 1 cut(s) 230
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 2173, 2205
FaqI GGGAC 2 cut(s) 1759, 2269
FbaI TGATCA 2 cut(s) 1770, 1851
FblI GTMKAC 1 cut(s) 1171
Fnu4HI GCNGC 7 cut(s) 1000, 1198, 1493, 1496, 1699, 2049, 2998
FokI GGATG 3 cut(s) 1345, 2419, 2942
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 7 cut(s) 1000, 1198, 1493, 1496, 1699, 2049, 2998
FspBI CTAG 6 cut(s) 608, 671, 953, 1355, 1437, 2501
GluI GCNGC 7 cut(s) 1000, 1198, 1493, 1496, 1699, 2049, 2998
GsaI CCCAGC 1 cut(s) 517
HaeIII GGCC 3 cut(s) 1200, 1312, 1384
HapII CCGG 2 cut(s) 235, 2392
HincII GTYRAC 2 cut(s) 622, 2938
HindII GTYRAC 2 cut(s) 622, 2938
HindIII AAGCTT 5 cut(s) 410, 451, 845, 1262, 2350
HinfI GANTC 8 cut(s) 98, 523, 676, 1141, 1757, 1858, 2117, 2395
HpaII CCGG 2 cut(s) 235, 2392
HphI GGTGA 6 cut(s) 276, 529, 1124, 2185, 2234, 2339
HpyCH4IV ACGT 3 cut(s) 223, 2631, 2661
HpyF10VI GCNNNNNNNGC 3 cut(s) 1390, 2054, 2663
HpyF3I CTNAG 6 cut(s) 75, 557, 1019, 1418, 2150, 2888
HpySE526I ACGT 3 cut(s) 223, 2631, 2661
Ksp22I TGATCA 2 cut(s) 1770, 1851
Kzo9I GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
LmnI GCTCC 4 cut(s) 136, 656, 2510, 3107
Lsp1109I GCAGC 5 cut(s) 1479, 1482, 1685, 2060, 2984
LweI GCATC 3 cut(s) 1326, 2491, 2984
MaeI CTAG 6 cut(s) 608, 671, 953, 1355, 1437, 2501
MaeII ACGT 3 cut(s) 223, 2631, 2661
MaeIII GTNAC 8 cut(s) 728, 865, 1112, 1204, 1797, 2342, 2468, 2753
MalI GATC 8 cut(s) 150, 641, 1503, 1772, 1853, 2164, 2746, 2886
MboI GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
MfeI CAATTG 2 cut(s) 1644, 2280
MflI RGATCY 2 cut(s) 639, 2162
MhlI GDGCHC 4 cut(s) 404, 2832, 2947, 3032
MlyI GAGTC 1 cut(s) 1766
MmeI TCCRAC 2 cut(s) 1008, 1752
MslI CAYNNNNRTG 1 cut(s) 3005
MspA1I CMGCKG 4 cut(s) 889, 1417, 2051, 2696
MspCI CTTAAG 1 cut(s) 1192
MspI CCGG 2 cut(s) 235, 2392
MspR9I CCNGG 6 cut(s) 236, 429, 1202, 2246, 2422, 2493
MunI CAATTG 2 cut(s) 1644, 2280
Mva1269I GAATGC 4 cut(s) 423, 2151, 2369, 3000
MvaI CCWGG 5 cut(s) 429, 1202, 2246, 2422, 2493
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 3 cut(s) 1390, 2054, 2663
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 343
NdeII GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
NlaIV GGNNCC 4 cut(s) 341, 1675, 2164, 2257
NmeAIII GCCGAG 1 cut(s) 489
NmuCI GTSAC 3 cut(s) 865, 1112, 1797
NspI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
NspV TTCGAA 3 cut(s) 1268, 1583, 1596
PaeR7I CTCGAG 1 cut(s) 2831
PciI ACATGT 2 cut(s) 831, 1722
PcsI WCGNNNNNNNCGW 1 cut(s) 2182
PctI GAATGC 4 cut(s) 423, 2151, 2369, 3000
PfeI GAWTC 7 cut(s) 98, 523, 676, 1141, 1858, 2117, 2395
PflMI CCANNNNNTGG 4 cut(s) 653, 1904, 2771, 2926
PkrI GCNGC 7 cut(s) 1001, 1199, 1494, 1497, 1700, 2050, 2999
PleI GAGTC 1 cut(s) 1765
PmaCI CACGTG 1 cut(s) 2662
PmlI CACGTG 1 cut(s) 2662
PpsI GAGTC 1 cut(s) 1765
Ppu21I YACGTR 1 cut(s) 2662
PpuMI RGGWCCY 1 cut(s) 2256
PscI ACATGT 2 cut(s) 831, 1722
PsiI TTATAA 2 cut(s) 1152, 1875
Psp124BI GAGCTC 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 2256
Psp6I CCWGG 5 cut(s) 427, 1200, 2244, 2420, 2491
PspCI CACGTG 1 cut(s) 2662
PspEI GGTNACC 2 cut(s) 1112, 1204
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 5 cut(s) 427, 1200, 2244, 2420, 2491
PspN4I GGNNCC 4 cut(s) 341, 1675, 2164, 2257
PspPI GGNCC 4 cut(s) 1310, 1382, 1674, 2256
PspPPI RGGWCCY 1 cut(s) 2256
PspXI VCTCGAGB 1 cut(s) 2831
PstI CTGCAG 2 cut(s) 721, 940
PstNI CAGNNNCTG 2 cut(s) 1904, 3082
PsuI RGATCY 2 cut(s) 639, 2162
PvuII CAGCTG 3 cut(s) 889, 1417, 2696
RsaI GTAC 8 cut(s) 39, 830, 1214, 1539, 1638, 2901, 2912, 2965
RsaNI GTAC 8 cut(s) 38, 829, 1213, 1538, 1637, 2900, 2911, 2964
RseI CAYNNNNRTG 1 cut(s) 3005
SacI GAGCTC 1 cut(s) 404
SatI GCNGC 7 cut(s) 1000, 1198, 1493, 1496, 1699, 2049, 2998
Sau3AI GATC 8 cut(s) 148, 639, 1501, 1770, 1851, 2162, 2744, 2884
Sau96I GGNCC 4 cut(s) 1310, 1382, 1674, 2256
SbfI CCTGCAGG 1 cut(s) 940
SchI GAGTC 1 cut(s) 1766
ScrFI CCNGG 6 cut(s) 236, 429, 1202, 2246, 2422, 2493
SdaI CCTGCAGG 1 cut(s) 940
SduI GDGCHC 4 cut(s) 404, 2832, 2947, 3032
SfaNI GCATC 3 cut(s) 1326, 2491, 2984
SfcI CTRYAG 5 cut(s) 717, 936, 2544, 2550, 2809
Sfr274I CTCGAG 1 cut(s) 2831
SfuI TTCGAA 3 cut(s) 1268, 1583, 1596
SinI GGWCC 2 cut(s) 1674, 2256
SlaI CTCGAG 1 cut(s) 2831
SmiMI CAYNNNNRTG 1 cut(s) 3005
SmlI CTYRAG 5 cut(s) 809, 962, 1192, 2831, 2987
SmoI CTYRAG 5 cut(s) 809, 962, 1192, 2831, 2987
Sse8387I CCTGCAGG 1 cut(s) 940
SsiI CCGC 5 cut(s) 999, 1197, 1391, 1985, 2051
SspMI CTAG 6 cut(s) 608, 671, 953, 1355, 1437, 2501
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 6 cut(s) 234, 427, 1200, 2244, 2420, 2491
StyI CCWWGG 3 cut(s) 343, 363, 1345
TaiI ACGT 3 cut(s) 226, 2634, 2664
TatI WGTACW 5 cut(s) 828, 1212, 1537, 1636, 2963
TauI GCSGC 2 cut(s) 1002, 1200
TfiI GAWTC 7 cut(s) 98, 523, 676, 1141, 1858, 2117, 2395
TseFI GTSAC 3 cut(s) 865, 1112, 1797
TseI GCWGC 5 cut(s) 1492, 1495, 1698, 2048, 2997
Tsp45I GTSAC 3 cut(s) 865, 1112, 1797
TspDTI ATGAA 7 cut(s) 43, 280, 423, 1038, 2247, 2724, 3100
TspGWI ACGGA 2 cut(s) 535, 608
Van91I CCANNNNNTGG 4 cut(s) 653, 1904, 2771, 2926
Vha464I CTTAAG 1 cut(s) 1192
VneI GTGCAC 2 cut(s) 2943, 3028
VpaK11BI GGWCC 2 cut(s) 1674, 2256
XapI RAATTY 9 cut(s) 125, 351, 394, 967, 1242, 1270, 1358, 1558, 2601
XbaI TCTAGA 1 cut(s) 1436
XceI RCATGY 5 cut(s) 216, 835, 1726, 2517, 2720
XhoI CTCGAG 1 cut(s) 2831
XmiI GTMKAC 1 cut(s) 1171
XspI CTAG 6 cut(s) 608, 671, 953, 1355, 1437, 2501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.