Rroxscaffold_6G00424710
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013738
Physical Location & Seq
Forward (+)
45849398 .. 45852637
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424710.1

Sequence Viewer

Length: 3240 bp
ATGAAGAAAGAAGCCAGAGAAAGAATGGCATACAATCTGAACTATTGTATAACAGAAATGGTACTAACCGTACTATGTTTTTTCTTCATTTTTCTTGTTCTGCCATCTAAGTCTGCATTTCTTTGTGAAGATTCCAGTGATTGCAACGCTCTGCTCAAATTCAAGGAGGAGCTAACAAGTGATCCCGAAGGTCATCTTCAAACTTGGAACGAAGCCAATCCCCTCTGCAACTGGACTGGAATTACATGCCACCAACGTCTCCAAAACCGGGTTATAGGCCTTGAACTGATAGATATGGGCTTGCAAGGAAGGATATCACCATTCATATCCAACCTCTCTCTTCTTACCAACCTGTCTTTACAGAGCAATGGGTTCCACGGACAAATTCCATCTTCCTTGGGAATGCTTAAAGAGTTGGCATTTCTGAATGTGAGCTCAAACAAGCTTGAAGGTAGCATTCCAGTTTCATTACATGGTTGCCAAAGCTTGAAAGTCATAGACATGAATAACAACAATCTATCTGGTGTTCTTCCTAAAGAACTCGGCTCGATGAAGAATCTAACATACTTGGGTCTCTCCGTAAACAATCTCAGCGGACTGCTTCCAGCCTCGCTTTCAAACTTAACAGAATTGACAAAACTACTACTGGGTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTTGGAGCATTAAGAAAGCTAGAGATTCTGTATCTCCATACAAACTACCTCGAAGGCTCAATACCTGCAGTAATTGGTAACTGCACTGCTTTACGCGAAATCACTCTGATTGGGAATATGTTAAATGGAGAAATCCCTTCAGATTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACTTGTCAAAAAACAAGCTTTCTGGGAGAATTCCAGTGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGGAAGTTCCTGCAGGATTGGGCAAGCTAGAGAACCTTGAGAATTTCTACTTACATACCAACAACTTGGGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTGACTGCCCTGACTAACTGTTCATATCTGCAAAAACTACACTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCAATAGGTGACCTTTCAAAAGACCTCTATTACTTGAATCTTTTTAATAACCACATTACAGGAAGCATACCAGATAGTATCGGAAACTTAAGCGGCCTGGTAACCTTGTACTTGTCATACAACCGTTTGGAAGGAAAAATTCCACCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTTATACTTGGGGAACAACATAATTATTGGGCCAATGCCAAAAGACTTGGGGATGATGCCTAACCTTGGTTTACTAGAACTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTTACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGGAAAGTTTCCGATTGAGCTTACTCACTGCTCTCTTATGATGCTGCTTGATCTATCCTTCAACAACTTACAGGGCTCTGTTCCTGTACAGATTGACCATTTTTCCAATTTAGCTCTCTCTCTTAATCTTTCGAACAATAATTTCGAAGGACAGTTGCCTGCAAGCATTGAAAAACTGGTTTCTGTACTGGCAATTGACTTGTCTGACAACCAGTTTTCTGGGTCCATACCTACATTGATCGGAAGCTGCATATCTTTGGTGTATCTAAACATGTCCAACAACATATTTGAGGGGACTATTCCAGAGTCGTTGAAGTTGATCACTTCACTTCAAGTTTTGGACTTGTCACTTAATCGATTAAATGGCAGTATTCCAATCTGGATTGCTAAAGCACAGATGATCAAGGATCTCAATTTATCTTATAACAGGTTATCAGGAGAAATTCCAGACACTGGGAGGATAAAATCTTTCAATAGAAGCTCATTCTTGGGGAATGTGGGGTTGTGTGGTGGTTCGGCAATACTTGGTCGTCGGCCTTGCGTAGTTCAAAAGAGAAAATATAAGATAAGAAAATCCATCCTTTACTCTTTGGTTGCAGCAGTTGCAGTAGTCTGTGTGCTGGTTGCTGTCTTTGTTTATTGTTTCTTCTTCTTCAGAAAACGGGATTCAAATCCAGAACACCCTGAACACAGAATGTTAAGAGTAATGGGATCACCGAGCCATCATGGAAGTCGAATTTTCACCCAAAGGGAACTTGAAATTGCAACAGGTGAGTTCAATGAAGCTCATCTCCTGGGTAGAGGAACCAGCGGAACAGTATATAAGGCAGTTATTGACAACGGGGAAAATATCGTGGCAGTGAAGGTACTGCATGGTGATGGTAATCAGAGTTACAGAAGCTTCAAGAGGGAATGTCAAATCATGTCCCAAGTTAAGCACCGGAATCTTGTAAGAATGGTAGGATATACCTGGAACGAACAGTTCAAGGCTCTTATTCTCGAGTATATTGACAATGGTAACTTAGCACAGCATCTTTATCCTGGTGAACTGGAGGAAGGAGCATGTGAATTATCGTTAAGGGATAGAATGTCAATAGCGATAGATGTTGCCAGTGGCTTGGAGTATCTTCAGGAAGGTTGTCCAGTCCAAATTATGCATTGTGATCTAAAACCAGAAAATGTGCTTATTGACAACAATATGGTGGCTCATGTAGCAGATTTCGGGATTGGGAAGCTCAACGCAGCTGAGAAATCCAATGAATCACATGTTAGCACAACACATTTTCTACGTGGATCAATTGGTTACATTCCCCCAGGTATGCATAATCACATCACTAGTTTCACATCTATAGAGCAAACATTGTGGTTAATAGAGTTTACTTGTATTACAGAATATGGACAAGGAATTGAGGTATCAGCAAAAGGAGACATCTATAGCTTCGGTGTCATGGTGCTTGAGATGATAACAAGAAGAAGGCCGACAAGCAATATGTTTCCGGATGGGCTTGATCTAAGGAAGTGGGTGGTTTCTTCGTTCCCAGACCATGTGTGGGATGTTGTTGACAGTACGTTAAAGGAGATACAAAGCACACATGGCGCTTTGGATGAGCTTGAGAAATGCTGCATTCAAATGCTTGATCTGGGGTTGATGTGCACAGAAGAGAATCCACAAGAAAGACCTGCTATGTCTTTTGTTGTGCAGAAGTTGAAACAGGTTATGAAAAGCTCCAATATCATTTTAGACATGAAGAGATGA

Protein Analysis

1079

Amino Acids

119.47

Weight (kDa)

6.71

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 83 2.8e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 155 - 265 2.4e-09 Leucine-rich repeat region
LRR_14 PF23598 351 - 490 6.8e-12 Leucine-rich repeat region
LRR_8 PF13855 388 - 444 1.1e-07 Leucine rich repeat
LRR_4 PF12799 412 - 446 2.4e-06 Leucine Rich repeats (2 copies)
Pkinase PF00069 755 - 1061 7.3e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 758 - 1064 1.2e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1908
Acc36I ACCTGC 2 cut(s) 757, 3172
AccB7I CCANNNNNTGG 2 cut(s) 686, 1937
AccII CGCG 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 2980
AciI CCGC 5 cut(s) 594, 1032, 1230, 1424, 2295
AclWI GGATC 5 cut(s) 176, 667, 1899, 2203, 2785
AcsI RAATTY 8 cut(s) 158, 384, 891, 1000, 1275, 1303, 1926, 2220
AcuI CTGAAG 3 cut(s) 807, 2122, 2597
AfaI GTAC 8 cut(s) 63, 72, 863, 1247, 1572, 1671, 2352, 3052
AfiI CCNNNNNNNGG 7 cut(s) 268, 686, 1289, 1379, 1433, 1937, 3034
AflII CTTAAG 1 cut(s) 1225
AflIII ACRYGT 2 cut(s) 1755, 2749
AhlI ACTAGT 1 cut(s) 2819
AjnI CCWGG 5 cut(s) 1233, 2277, 2453, 2524, 2797
Alw21I GWGCWC 2 cut(s) 437, 3140
Alw26I GTCTC 3 cut(s) 263, 578, 2904
Alw44I GTGCAC 1 cut(s) 3136
AlwI GGATC 5 cut(s) 176, 667, 1899, 2203, 2785
AlwNI CAGNNNCTG 1 cut(s) 1937
Ama87I CYCGRG 1 cut(s) 2483
Aor13HI TCCGGA 1 cut(s) 2980
AoxI GGCC 6 cut(s) 277, 1231, 1343, 1415, 2018, 2960
ApaLI GTGCAC 1 cut(s) 3136
ApeKI GCWGC 5 cut(s) 1528, 1731, 2081, 2726, 3105
ApoI RAATTY 8 cut(s) 158, 384, 891, 1000, 1275, 1303, 1926, 2220
AspLEI GCGC 1 cut(s) 3083
AspS9I GGNCC 3 cut(s) 1343, 1415, 1707
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 7 cut(s) 309, 1157, 2190, 2218, 2267, 2372, 2540
AsuII TTCGAA 3 cut(s) 1301, 1616, 1629
AvaI CYCGRG 1 cut(s) 2483
AvaII GGWCC 1 cut(s) 1707
BaeGI GKGCMC 1 cut(s) 3140
BanII GRGCYC 2 cut(s) 437, 1562
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
Bbv12I GWGCWC 2 cut(s) 437, 3140
BbvI GCAGC 5 cut(s) 1515, 1718, 2093, 2738, 3092
BccI CCATC 7 cut(s) 112, 397, 1141, 2069, 2214, 2357, 2978
BciT130I CCWGG 5 cut(s) 1235, 2279, 2455, 2526, 2799
BclI TGATCA 2 cut(s) 1803, 1884
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 3 cut(s) 263, 578, 2904
BcuI ACTAGT 1 cut(s) 2819
BfaI CTAG 5 cut(s) 704, 986, 1388, 1470, 2820
BfmI CTRYAG 4 cut(s) 750, 969, 2832, 2917
BfoI RGCGCY 1 cut(s) 3084
BfrI CTTAAG 1 cut(s) 1225
BfuAI ACCTGC 2 cut(s) 757, 3172
BisI GCNGC 7 cut(s) 1033, 1231, 1529, 1732, 2082, 2727, 3106
BlsI GCNGC 7 cut(s) 1034, 1232, 1530, 1733, 2083, 2728, 3107
Bme1390I CCNGG 6 cut(s) 269, 1235, 2279, 2455, 2526, 2799
Bme18I GGWCC 1 cut(s) 1707
BmeT110I CYCGRG 1 cut(s) 2483
BmgT120I GGNCC 3 cut(s) 1343, 1415, 1707
BmiI GGNNCC 3 cut(s) 374, 1708, 2290
BmrFI CCNGG 6 cut(s) 269, 1235, 2279, 2455, 2526, 2799
BmrI ACTGGG 2 cut(s) 656, 1947
BmsI GCATC 3 cut(s) 1359, 1515, 2524
BmuI ACTGGG 2 cut(s) 656, 1947
BpmI CTGGAG 1 cut(s) 2555
Bpu14I TTCGAA 3 cut(s) 1301, 1616, 1629
BpuEI CTTGAG 4 cut(s) 827, 1016, 2960, 3116
BpuMI CCSGG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 1840
BsaAI YACGTR 1 cut(s) 2774
BsaBI GATNNNNATC 2 cut(s) 2154, 2367
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 5 cut(s) 376, 396, 1378, 2278, 2797
BsaWI WCCGGW 2 cut(s) 2424, 2980
Bsc4I CCNNNNNNNGG 7 cut(s) 268, 686, 1289, 1379, 1433, 1937, 3034
Bse3DI GCAATG 1 cut(s) 373
Bse8I GATNNNNATC 2 cut(s) 2154, 2367
BseAI TCCGGA 1 cut(s) 2980
BseBI CCWGG 5 cut(s) 1235, 2279, 2455, 2526, 2799
BseCI ATCGAT 1 cut(s) 1840
BseDI CCNNGG 5 cut(s) 376, 396, 1378, 2278, 2797
BseGI GGATG 5 cut(s) 1371, 2061, 2989, 3043, 3094
BseJI GATNNNNATC 2 cut(s) 2154, 2367
BseLI CCNNNNNNNGG 7 cut(s) 268, 686, 1289, 1379, 1433, 1937, 3034
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 4 cut(s) 604, 1066, 1442, 2721
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 3140
BseXI GCAGC 5 cut(s) 1515, 1718, 2093, 2738, 3092
BsgI GTGCAG 2 cut(s) 751, 3203
Bsh1236I CGCG 1 cut(s) 780
BshFI GGCC 6 cut(s) 279, 1233, 1345, 1417, 2020, 2962
BshVI ATCGAT 1 cut(s) 1840
BsiHKAI GWGCWC 2 cut(s) 437, 3140
BsiHKCI CYCGRG 1 cut(s) 2483
BsiSI CCGG 3 cut(s) 268, 2425, 2981
BslFI GGGAC 2 cut(s) 1792, 2395
BslI CCNNNNNNNGG 7 cut(s) 268, 686, 1289, 1379, 1433, 1937, 3034
BsmAI GTCTC 3 cut(s) 263, 578, 2904
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 1792, 2395
BsmI GAATGC 3 cut(s) 408, 456, 3108
BsnI GGCC 6 cut(s) 279, 1233, 1345, 1417, 2020, 2962
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 1 cut(s) 2483
Bsp119I TTCGAA 3 cut(s) 1301, 1616, 1629
Bsp1286I GDGCHC 3 cut(s) 437, 1562, 3140
Bsp13I TCCGGA 1 cut(s) 2980
Bsp1407I TGTACA 1 cut(s) 1570
BspACI CCGC 5 cut(s) 594, 1032, 1230, 1424, 2295
BspANI GGCC 6 cut(s) 279, 1233, 1345, 1417, 2020, 2962
BspCNI CTCAG 4 cut(s) 603, 1065, 1443, 2722
BspDI ATCGAT 1 cut(s) 1840
BspEI TCCGGA 1 cut(s) 2980
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 3 cut(s) 374, 1708, 2290
BspMAI CTGCAG 2 cut(s) 754, 973
BspMI ACCTGC 2 cut(s) 757, 3172
BspPI GGATC 5 cut(s) 176, 667, 1899, 2203, 2785
BspT104I TTCGAA 3 cut(s) 1301, 1616, 1629
BspTI CTTAAG 1 cut(s) 1225
BspTNI GGTCTC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 1570
BssECI CCNNGG 5 cut(s) 376, 396, 1378, 2278, 2797
BssT1I CCWWGG 2 cut(s) 396, 1378
Bst2UI CCWGG 5 cut(s) 1235, 2279, 2455, 2526, 2799
Bst4CI ACNGT 8 cut(s) 70, 930, 1079, 1262, 1638, 2302, 2466, 3050
Bst6I CTCTTC 3 cut(s) 345, 3138, 3227
BstAFI CTTAAG 1 cut(s) 1225
BstAPI GCANNNNNTGC 1 cut(s) 2087
BstAUI TGTACA 1 cut(s) 1570
BstBAI YACGTR 1 cut(s) 2774
BstBI TTCGAA 3 cut(s) 1301, 1616, 1629
BstC8I GCNNGC 5 cut(s) 302, 983, 1295, 1644, 1648
BstDEI CTNAG 7 cut(s) 108, 590, 1052, 1451, 2506, 2730, 2996
BstDSI CCRYGG 1 cut(s) 376
BstEII GGTNACC 2 cut(s) 1145, 1237
BstF5I GGATG 5 cut(s) 1371, 2061, 2989, 3043, 3094
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 3084
BstHHI GCGC 1 cut(s) 3083
BstMAI GTCTC 3 cut(s) 263, 578, 2904
BstMWI GCNNNNNNNGC 4 cut(s) 1423, 2087, 2696, 3078
BstNI CCWGG 5 cut(s) 1235, 2279, 2455, 2526, 2799
BstNSI RCATGY 4 cut(s) 249, 1759, 2550, 2753
BstPI GGTNACC 2 cut(s) 1145, 1237
BstSCI CCNGG 6 cut(s) 267, 1233, 2277, 2453, 2524, 2797
BstSFI CTRYAG 4 cut(s) 750, 969, 2832, 2917
BstSLI GKGCMC 1 cut(s) 3140
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 5 cut(s) 1515, 1718, 2093, 2738, 3092
BstX2I RGATCY 2 cut(s) 672, 1891
BstXI CCANNNNNNTGG 3 cut(s) 1024, 1703, 2602
BstYI RGATCY 2 cut(s) 672, 1891
Bsu15I ATCGAT 1 cut(s) 1840
BsuRI GGCC 6 cut(s) 279, 1233, 1345, 1417, 2020, 2962
BsuTUI ATCGAT 1 cut(s) 1840
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 5 cut(s) 1371, 2061, 2989, 3043, 3094
BtsI GCAGTG 3 cut(s) 768, 1510, 2349
BtsIMutI CAGTG 8 cut(s) 142, 768, 903, 1119, 1510, 1935, 2349, 2602
BveI ACCTGC 2 cut(s) 757, 3172
Cac8I GCNNGC 5 cut(s) 302, 983, 1295, 1644, 1648
CaiI CAGNNNCTG 1 cut(s) 1937
CfoI GCGC 1 cut(s) 3083
Cfr13I GGNCC 3 cut(s) 1343, 1415, 1707
ClaI ATCGAT 1 cut(s) 1840
Csp6I GTAC 8 cut(s) 62, 71, 862, 1246, 1571, 1670, 2351, 3051
CspCI CAANNNNNGTGG 4 cut(s) 1009, 1044, 2828, 2863
CviQI GTAC 8 cut(s) 62, 71, 862, 1246, 1571, 1670, 2351, 3051
DdeI CTNAG 7 cut(s) 108, 590, 1052, 1451, 2506, 2730, 2996
Eam1104I CTCTTC 3 cut(s) 345, 3138, 3227
EarI CTCTTC 3 cut(s) 345, 3138, 3227
EciI GGCGGA 1 cut(s) 1413
Ecl136II GAGCTC 1 cut(s) 435
Eco130I CCWWGG 2 cut(s) 396, 1378
Eco147I AGGCCT 1 cut(s) 279
Eco24I GRGCYC 2 cut(s) 437, 1562
Eco31I GGTCTC 1 cut(s) 578
Eco32I GATATC 2 cut(s) 315, 1457
Eco47I GGWCC 1 cut(s) 1707
Eco53kI GAGCTC 1 cut(s) 435
Eco57I CTGAAG 3 cut(s) 807, 2122, 2597
Eco88I CYCGRG 1 cut(s) 2483
Eco91I GGTNACC 2 cut(s) 1145, 1237
EcoICRI GAGCTC 1 cut(s) 435
EcoO65I GGTNACC 2 cut(s) 1145, 1237
EcoRI GAATTC 1 cut(s) 891
EcoRII CCWGG 5 cut(s) 1233, 2277, 2453, 2524, 2797
EcoRV GATATC 2 cut(s) 315, 1457
EcoT14I CCWWGG 2 cut(s) 396, 1378
EcoT22I ATGCAT 2 cut(s) 2643, 2808
EcoT38I GRGCYC 2 cut(s) 437, 1562
ErhI CCWWGG 2 cut(s) 396, 1378
Esp3I CGTCTC 1 cut(s) 263
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 2 cut(s) 1792, 2395
FbaI TGATCA 2 cut(s) 1803, 1884
Fnu4HI GCNGC 7 cut(s) 1033, 1231, 1529, 1732, 2082, 2727, 3106
FokI GGATG 5 cut(s) 1378, 2048, 2996, 3050, 3101
FriOI GRGCYC 2 cut(s) 437, 1562
Fsp4HI GCNGC 7 cut(s) 1033, 1231, 1529, 1732, 2082, 2727, 3106
FspBI CTAG 5 cut(s) 704, 986, 1388, 1470, 2820
GlaI GCGC 1 cut(s) 3082
GluI GCNGC 7 cut(s) 1033, 1231, 1529, 1732, 2082, 2727, 3106
GsuI CTGGAG 1 cut(s) 2555
HaeII RGCGCY 1 cut(s) 3084
HaeIII GGCC 6 cut(s) 279, 1233, 1345, 1417, 2020, 2962
HapII CCGG 3 cut(s) 268, 2425, 2981
HhaI GCGC 1 cut(s) 3083
Hin6I GCGC 1 cut(s) 3081
HinP1I GCGC 1 cut(s) 3081
HincII GTYRAC 2 cut(s) 655, 3046
HindII GTYRAC 2 cut(s) 655, 3046
HindIII AAGCTT 5 cut(s) 443, 484, 878, 1295, 2383
HinfI GANTC 9 cut(s) 131, 556, 709, 1174, 1790, 2150, 2428, 2744, 3148
HpaII CCGG 3 cut(s) 268, 2425, 2981
HphI GGTGA 7 cut(s) 309, 1157, 2190, 2218, 2267, 2372, 2540
Hpy166II GTNNAC 8 cut(s) 583, 655, 1119, 1385, 2531, 2862, 3046, 3138
Hpy8I GTNNAC 8 cut(s) 583, 655, 1119, 1385, 2531, 2862, 3046, 3138
Hpy99I CGWCG 1 cut(s) 2019
HpyCH4III ACNGT 8 cut(s) 70, 930, 1079, 1262, 1638, 2302, 2466, 3050
HpyCH4IV ACGT 3 cut(s) 256, 2773, 3053
HpyF10VI GCNNNNNNNGC 4 cut(s) 1423, 2087, 2696, 3078
HpyF3I CTNAG 7 cut(s) 108, 590, 1052, 1451, 2506, 2730, 2996
HpySE526I ACGT 3 cut(s) 256, 2773, 3053
HspAI GCGC 1 cut(s) 3081
Kpn2I TCCGGA 1 cut(s) 2980
Ksp22I TGATCA 2 cut(s) 1803, 1884
LmnI GCTCC 4 cut(s) 169, 689, 2543, 3215
Lsp1109I GCAGC 5 cut(s) 1515, 1718, 2093, 2738, 3092
LweI GCATC 3 cut(s) 1359, 1515, 2524
MaeI CTAG 5 cut(s) 704, 986, 1388, 1470, 2820
MaeII ACGT 3 cut(s) 256, 2773, 3053
MaeIII GTNAC 8 cut(s) 761, 898, 1145, 1237, 1830, 2375, 2501, 2786
MfeI CAATTG 2 cut(s) 1677, 2781
MflI RGATCY 2 cut(s) 672, 1891
MhlI GDGCHC 3 cut(s) 437, 1562, 3140
MlyI GAGTC 1 cut(s) 1799
MmeI TCCRAC 2 cut(s) 354, 1785
Mph1103I ATGCAT 2 cut(s) 2643, 2808
MroI TCCGGA 1 cut(s) 2980
MslI CAYNNNNRTG 3 cut(s) 500, 2361, 3113
MspA1I CMGCKG 5 cut(s) 594, 922, 1450, 2295, 2729
MspCI CTTAAG 1 cut(s) 1225
MspI CCGG 3 cut(s) 268, 2425, 2981
MspR9I CCNGG 6 cut(s) 269, 1235, 2279, 2455, 2526, 2799
MunI CAATTG 2 cut(s) 1677, 2781
Mva1269I GAATGC 3 cut(s) 408, 456, 3108
MvaI CCWGG 5 cut(s) 1235, 2279, 2455, 2526, 2799
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 4 cut(s) 1423, 2087, 2696, 3078
NciI CCSGG 1 cut(s) 269
NlaIV GGNNCC 3 cut(s) 374, 1708, 2290
NmeAIII GCCGAG 1 cut(s) 522
NmuCI GTSAC 3 cut(s) 898, 1145, 1830
NsiI ATGCAT 2 cut(s) 2643, 2808
NspI RCATGY 4 cut(s) 249, 1759, 2550, 2753
NspV TTCGAA 3 cut(s) 1301, 1616, 1629
PaeR7I CTCGAG 1 cut(s) 2483
PceI AGGCCT 1 cut(s) 279
PciI ACATGT 2 cut(s) 1755, 2749
PctI GAATGC 3 cut(s) 408, 456, 3108
PfeI GAWTC 8 cut(s) 131, 556, 709, 1174, 2150, 2428, 2744, 3148
PflMI CCANNNNNTGG 2 cut(s) 686, 1937
PkrI GCNGC 7 cut(s) 1034, 1232, 1530, 1733, 2083, 2728, 3107
PleI GAGTC 1 cut(s) 1798
PpsI GAGTC 1 cut(s) 1798
Ppu21I YACGTR 1 cut(s) 2774
PscI ACATGT 2 cut(s) 1755, 2749
PsiI TTATAA 1 cut(s) 1908
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 5 cut(s) 1233, 2277, 2453, 2524, 2797
PspEI GGTNACC 2 cut(s) 1145, 1237
PspGI CCWGG 5 cut(s) 1233, 2277, 2453, 2524, 2797
PspN4I GGNNCC 3 cut(s) 374, 1708, 2290
PspPI GGNCC 3 cut(s) 1343, 1415, 1707
PstI CTGCAG 2 cut(s) 754, 973
PstNI CAGNNNCTG 1 cut(s) 1937
PsuI RGATCY 2 cut(s) 672, 1891
PvuII CAGCTG 3 cut(s) 922, 1450, 2729
RsaI GTAC 8 cut(s) 63, 72, 863, 1247, 1572, 1671, 2352, 3052
RsaNI GTAC 8 cut(s) 62, 71, 862, 1246, 1571, 1670, 2351, 3051
RseI CAYNNNNRTG 3 cut(s) 500, 2361, 3113
SacI GAGCTC 1 cut(s) 437
SatI GCNGC 7 cut(s) 1033, 1231, 1529, 1732, 2082, 2727, 3106
Sau96I GGNCC 3 cut(s) 1343, 1415, 1707
SbfI CCTGCAGG 1 cut(s) 973
SchI GAGTC 1 cut(s) 1799
ScrFI CCNGG 6 cut(s) 269, 1235, 2279, 2455, 2526, 2799
SdaI CCTGCAGG 1 cut(s) 973
SduI GDGCHC 3 cut(s) 437, 1562, 3140
SfaNI GCATC 3 cut(s) 1359, 1515, 2524
SfcI CTRYAG 4 cut(s) 750, 969, 2832, 2917
Sfr274I CTCGAG 1 cut(s) 2483
SfuI TTCGAA 3 cut(s) 1301, 1616, 1629
SinI GGWCC 1 cut(s) 1707
SlaI CTCGAG 1 cut(s) 2483
SmiMI CAYNNNNRTG 3 cut(s) 500, 2361, 3113
SmlI CTYRAG 6 cut(s) 842, 995, 1225, 2483, 2939, 3095
SmoI CTYRAG 6 cut(s) 842, 995, 1225, 2483, 2939, 3095
SpeI ACTAGT 1 cut(s) 2819
Sse8387I CCTGCAGG 1 cut(s) 973
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 5 cut(s) 594, 1032, 1230, 1424, 2295
SspMI CTAG 5 cut(s) 704, 986, 1388, 1470, 2820
SstI GAGCTC 1 cut(s) 437
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 6 cut(s) 267, 1233, 2277, 2453, 2524, 2797
StyI CCWWGG 2 cut(s) 396, 1378
TaaI ACNGT 8 cut(s) 70, 930, 1079, 1262, 1638, 2302, 2466, 3050
TaiI ACGT 3 cut(s) 259, 2776, 3056
TaqI TCGA 9 cut(s) 548, 735, 1301, 1406, 1616, 1629, 1840, 2218, 2484
TatI WGTACW 4 cut(s) 861, 1245, 1570, 1669
TauI GCSGC 2 cut(s) 1035, 1233
TfiI GAWTC 8 cut(s) 131, 556, 709, 1174, 2150, 2428, 2744, 3148
TscAI CASTG 8 cut(s) 142, 775, 903, 1126, 1517, 1942, 2349, 2602
TseFI GTSAC 3 cut(s) 898, 1145, 1830
TseI GCWGC 5 cut(s) 1528, 1731, 2081, 2726, 3105
Tsp45I GTSAC 3 cut(s) 898, 1145, 1830
TspGWI ACGGA 2 cut(s) 393, 568
TspRI CASTG 8 cut(s) 142, 775, 903, 1126, 1517, 1942, 2349, 2602
Van91I CCANNNNNTGG 2 cut(s) 686, 1937
Vha464I CTTAAG 1 cut(s) 1225
VneI GTGCAC 1 cut(s) 3136
VpaK11BI GGWCC 1 cut(s) 1707
XapI RAATTY 8 cut(s) 158, 384, 891, 1000, 1275, 1303, 1926, 2220
XbaI TCTAGA 1 cut(s) 1469
XceI RCATGY 4 cut(s) 249, 1759, 2550, 2753
XcmI CCANNNNNNNNNTGG 2 cut(s) 22, 3030
XhoI CTCGAG 1 cut(s) 2483
XspI CTAG 5 cut(s) 704, 986, 1388, 1470, 2820
Zsp2I ATGCAT 2 cut(s) 2643, 2808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.