RchiOBHm_Chr3g0476731

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0014841
Physical Location & Seq
Reverse (-)
22703275 .. 22704047
773 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44212

Sequence Viewer

Length: 726 bp
ATGGCCATATTACCAGCTGGGAGGTTGCAGATTATGCATCTCTCTTTTTTGGTTGTACTAGCAGCAGCTCAAACCCAGACTCCTCAAGTCAAGCTGAATTGCCCAGAGCACTGCGGTGATGTCCCAATTTCATATCCATTTGGCATTGGTGCCGACTGTTACCTCCGACCAGAGTTCAACATCACTTGTGACCAGCTCACCACACCCCCGTCACCAAAGTTCTCTAACAGTACATTCACCTTTCCCGTACGTATTACAAACTTTTCCCATGGTGAGGGTGAGTTGCAAGTAATACAAGACGTAGCCAGAGTGTGTTATAACACATACGGTTTTTACGAGGCGGATATGTCCACCAACAGCACGCTCGAGCTGCCACCTCCATACACCATCTCTCACAAGAACAAGTTCTTCGCAGTGGGGTGTAACAGTGTAGCAGTGTATCAAGGTTACCAAAGCAAAGTCCCAGATCCAGAAGAAAGCTACAAAGGCGGGTACACCGTGAGCCGGTGTCTAGATGCTCTCGGCACAGCACTTCCCACCAATTCTTGCAATGGTTTCGGGTGTGGCCTAAATCCGTTCCCTAGTGGACTGCAAAACATGTCGGTGGAATTGTATACACTTGATAGCGCCATCAGATGGTGGAACCACTGGAACCTAGAGCCAGACTATCTGTGCAGCTATGGCTTCATTGTGCAAGATGACAACAGTCAAATAAGACAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

241

Amino Acids

26.82

Weight (kDa)

4.98

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 97 8e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 636
AccI GTMKAC 1 cut(s) 614
AciI CCGC 3 cut(s) 114, 341, 489
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 4 cut(s) 57, 232, 249, 494
AfiI CCNNNNNNNGG 3 cut(s) 274, 504, 636
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 1 cut(s) 178
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 7 cut(s) 17, 68, 94, 196, 370, 480, 678
AluI AGCT 7 cut(s) 17, 68, 94, 196, 370, 480, 678
Alw21I GWGCWC 1 cut(s) 111
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 2 cut(s) 3, 565
ApeKI GCWGC 4 cut(s) 62, 65, 370, 675
Asp700I GAANNNNTTC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 629
AsuHPI GGTGA 6 cut(s) 128, 190, 204, 229, 284, 290
AvaI CYCGRG 1 cut(s) 365
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 74, 77, 357, 687
BccI CCATC 3 cut(s) 395, 630, 638
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BfaI CTAG 4 cut(s) 59, 512, 582, 656
BfoI RGCGCY 1 cut(s) 630
BisI GCNGC 4 cut(s) 63, 66, 371, 676
BlsI GCNGC 4 cut(s) 64, 67, 372, 677
BmeT110I CYCGRG 1 cut(s) 365
BmiI GGNNCC 3 cut(s) 151, 644, 653
BmsI GCATC 2 cut(s) 46, 505
BoxI GACNNNNGTC 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 504, 636
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 653
Bse3DI GCAATG 1 cut(s) 556
BseDI CCNNGG 1 cut(s) 268
BseLI CCNNNNNNNGG 3 cut(s) 274, 504, 636
BseMI GCAATG 1 cut(s) 556
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 4 cut(s) 74, 77, 357, 687
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 694
BshFI GGCC 2 cut(s) 5, 567
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 1 cut(s) 505
BsiWI CGTACG 1 cut(s) 247
BslFI GGGAC 2 cut(s) 107, 446
BslI CCNNNNNNNGG 3 cut(s) 274, 504, 636
BsmFI GGGAC 2 cut(s) 107, 446
BsnI GGCC 2 cut(s) 5, 567
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 466
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 3 cut(s) 114, 341, 489
BspANI GGCC 2 cut(s) 5, 567
BspLI GGNNCC 3 cut(s) 151, 644, 653
BspPI GGATC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 653
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 466
BssNAI GTATAC 1 cut(s) 615
BssT1I CCWWGG 1 cut(s) 268
Bst1107I GTATAC 1 cut(s) 615
Bst4CI ACNGT 7 cut(s) 158, 230, 329, 428, 499, 707, 720
BstAPI GCANNNNNTGC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 362
BstDSI CCRYGG 1 cut(s) 268
BstEII GGTNACC 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 1 cut(s) 469
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 34, 370, 486, 681
BstNSI RCATGY 1 cut(s) 601
BstPAI GACNNNNGTC 1 cut(s) 705
BstPI GGTNACC 1 cut(s) 446
BstSNI TACGTA 1 cut(s) 251
BstV1I GCAGC 4 cut(s) 74, 77, 357, 687
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 1 cut(s) 615
BsuRI GGCC 2 cut(s) 5, 567
BtgI CCRYGG 1 cut(s) 268
BtsI GCAGTG 3 cut(s) 109, 420, 441
BtsIMutI CAGTG 5 cut(s) 109, 420, 433, 441, 646
Cac8I GCNNGC 1 cut(s) 362
CfoI GCGC 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 504
Csp6I GTAC 4 cut(s) 56, 231, 248, 493
CviAII CATG 2 cut(s) 269, 598
CviQI GTAC 4 cut(s) 56, 231, 248, 493
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 356
Eco105I TACGTA 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 268
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 268
EcoT22I ATGCAT 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 2 cut(s) 272, 601
FaqI GGGAC 2 cut(s) 107, 446
FatI CATG 2 cut(s) 268, 597
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 4 cut(s) 63, 66, 371, 676
Fsp4HI GCNGC 4 cut(s) 63, 66, 371, 676
FspBI CTAG 4 cut(s) 59, 512, 582, 656
GlaI GCGC 1 cut(s) 628
GluI GCNGC 4 cut(s) 63, 66, 371, 676
GsaI CCCAGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 2 cut(s) 5, 567
HapII CCGG 1 cut(s) 505
HhaI GCGC 1 cut(s) 629
Hin1II CATG 2 cut(s) 272, 601
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HinfI GANTC 1 cut(s) 79
HpaII CCGG 1 cut(s) 505
HphI GGTGA 6 cut(s) 128, 190, 204, 229, 284, 290
Hpy166II GTNNAC 4 cut(s) 351, 495, 587, 615
Hpy188I TCNGA 2 cut(s) 167, 635
Hpy188III TCNNGA 2 cut(s) 470, 512
Hpy8I GTNNAC 4 cut(s) 351, 495, 587, 615
HpyCH4III ACNGT 7 cut(s) 158, 230, 329, 428, 499, 707, 720
HpyCH4IV ACGT 2 cut(s) 250, 300
HpyCH4V TGCA 7 cut(s) 28, 37, 286, 549, 592, 675, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 370, 486, 681
HpySE526I ACGT 2 cut(s) 250, 300
Hsp92II CATG 2 cut(s) 272, 601
HspAI GCGC 1 cut(s) 627
Kzo9I GATC 1 cut(s) 466
Lsp1109I GCAGC 4 cut(s) 74, 77, 357, 687
LweI GCATC 2 cut(s) 46, 505
MaeI CTAG 4 cut(s) 59, 512, 582, 656
MaeII ACGT 2 cut(s) 250, 300
MaeIII GTNAC 5 cut(s) 158, 188, 210, 422, 446
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 2 cut(s) 400, 485
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 97, 126, 541, 608
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 6 cut(s) 15, 93, 173, 268, 331, 387
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 404
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 114, 602
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 505
MwoI GCNNNNNNNGC 4 cut(s) 34, 370, 486, 681
NcoI CCATGG 1 cut(s) 268
NdeII GATC 1 cut(s) 466
NlaIII CATG 2 cut(s) 272, 601
NlaIV GGNNCC 3 cut(s) 151, 644, 653
NmeAIII GCCGAG 1 cut(s) 501
NmuCI GTSAC 2 cut(s) 188, 210
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 1 cut(s) 601
OliI CACNNNNGTG 1 cut(s) 114
PaeR7I CTCGAG 1 cut(s) 365
PciI ACATGT 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 404
Pfl23II CGTACG 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 636
PkrI GCNGC 4 cut(s) 64, 67, 372, 677
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 251
PscI ACATGT 1 cut(s) 597
PshAI GACNNNNGTC 1 cut(s) 705
PsiI TTATAA 1 cut(s) 318
PspEI GGTNACC 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 17
PspLI CGTACG 1 cut(s) 247
PspN4I GGNNCC 3 cut(s) 151, 644, 653
PspXI VCTCGAGB 1 cut(s) 365
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 4 cut(s) 57, 232, 249, 494
RsaNI GTAC 4 cut(s) 56, 231, 248, 493
RseI CAYNNNNRTG 2 cut(s) 114, 602
SatI GCNGC 4 cut(s) 63, 66, 371, 676
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 1 cut(s) 73
SduI GDGCHC 1 cut(s) 111
SfaNI GCATC 2 cut(s) 46, 505
Sfr274I CTCGAG 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 114, 602
SmlI CTYRAG 2 cut(s) 84, 365
SmoI CTYRAG 2 cut(s) 84, 365
SnaBI TACGTA 1 cut(s) 251
Sse9I AATT 4 cut(s) 97, 126, 541, 608
SsiI CCGC 3 cut(s) 114, 341, 489
SspMI CTAG 4 cut(s) 59, 512, 582, 656
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 7 cut(s) 158, 230, 329, 428, 499, 707, 720
TaiI ACGT 2 cut(s) 253, 303
TaqI TCGA 1 cut(s) 366
TasI AATT 4 cut(s) 97, 126, 541, 608
TatI WGTACW 2 cut(s) 55, 230
TscAI CASTG 5 cut(s) 116, 420, 433, 441, 653
TseFI GTSAC 2 cut(s) 188, 210
TseI GCWGC 4 cut(s) 62, 65, 370, 675
Tsp45I GTSAC 2 cut(s) 188, 210
TspDTI ATGAA 2 cut(s) 120, 676
TspGWI ACGGA 1 cut(s) 564
TspRI CASTG 5 cut(s) 116, 420, 433, 441, 653
Van91I CCANNNNNTGG 1 cut(s) 636
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 4 cut(s) 59, 512, 582, 656
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.