RchiOBHm_Chr5g0016921

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014841
Physical Location & Seq
Reverse (-)
11615216 .. 11616421
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29726

Sequence Viewer

Length: 879 bp
ATGGCCTTATTACCAGCTGGGAGGTTGCAAATCATGCATCTCTCTTATGTGGTTGTTCTATTAGTAGCAAAAGCTCAAGCCCCGCCGCCTCAAGCCAAGCCGAATTGCCCAGAGCGCTGCGGTGACGTCCCAATTCCATATCCATTTGGCATTGGTGCCGACTGTTACCTCCGACCAGAGTTCAACATCACTTGTGACCAGTACACCACACCCCCATCACCAAAGTTCTCCAACAGTACCTTCACCTTTCCTGTACGTATTACTGACTTTTCCCATGGTGAGGGTGAGTTGCAAGTAATGCAAGCCGTAGCTAGAGACTGTTATGACGAAGGCGGTTCGTACATGTACGATATGTATAGCAACAGCACGCTCAAGCTGCCTCTTTCGTACACTATATCTTATAAGAACAACTTCTTCGTGGTTGGTTGTAACAGTGTAGCAGTGTATCAAAGTTACCAGGGCCAAATCACCGATCGAGAAGAAAGCTACAAAGGCGGGTACACCGTGAGCCTGTGTCAAGATGCTCTTGGCACAGCACTTCCCACCGATTCTTGCAATGGTTTCGGGTGTGGCCAAAATGCAATCCCTAGTGGACTGCAAAACATGTCGGTGGATTTGTATCAACTTGACAGCTACATCAGAGATTGGAACCACTGGAACATAGAGCCAGACTACCTAGACTTATTCAAGCAAGATGGTACTTCATCTGGAGGTTACACTTGCCGGTGCATGACAGGCTACGATGGGAACCCATACCTCTCAGATGGTTGCCAAGATATTGATGAGTGCCTGCTAGTTCCGAACTCGAACCCATGCAACAAAGGAAAGTGCATAAATTTACCCGGAACTCACACTCGTGCATGGGTACAGATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

292

Amino Acids

32.32

Weight (kDa)

4.76

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 99 7.8e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 155
AciI CCGC 5 cut(s) 83, 86, 120, 333, 495
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 1 cut(s) 835
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 9 cut(s) 203, 238, 255, 341, 347, 389, 500, 700, 867
AfeI AGCGCT 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 280
AflIII ACRYGT 2 cut(s) 342, 603
AgsI TTSAA 2 cut(s) 184, 688
AjnI CCWGG 1 cut(s) 456
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AleI CACNNNNGTG 1 cut(s) 855
AluBI AGCT 6 cut(s) 17, 74, 311, 376, 486, 633
AluI AGCT 6 cut(s) 17, 74, 311, 376, 486, 633
Alw26I GTCTC 1 cut(s) 309
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 3 cut(s) 3, 460, 571
ApeKI GCWGC 2 cut(s) 117, 376
ApoI RAATTY 1 cut(s) 835
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 460
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 6 cut(s) 134, 210, 235, 290, 296, 460
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 855
BbvI GCAGC 2 cut(s) 104, 363
BccI CCATC 4 cut(s) 223, 689, 737, 758
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 1 cut(s) 843
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 4 cut(s) 312, 588, 677, 794
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 3 cut(s) 86, 118, 377
BlsI GCNGC 3 cut(s) 87, 119, 378
Bme1390I CCNGG 2 cut(s) 458, 843
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 157, 650, 749
BmrFI CCNGG 2 cut(s) 458, 843
BmsI GCATC 2 cut(s) 46, 511
BpmI CTGGAG 1 cut(s) 729
BpuEI CTTGAG 3 cut(s) 60, 75, 356
BpuMI CCSGG 1 cut(s) 843
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 274, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 723
Bse1I ACTGG 2 cut(s) 199, 659
Bse3DI GCAATG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 274, 457
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 2 cut(s) 199, 659
BseXI GCAGC 2 cut(s) 104, 363
BseYI CCCAGC 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 3 cut(s) 5, 462, 573
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 475
BsiSI CCGG 2 cut(s) 724, 843
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 3 cut(s) 5, 462, 573
Bsp143I GATC 1 cut(s) 472
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 5 cut(s) 83, 86, 120, 333, 495
BspANI GGCC 3 cut(s) 5, 462, 573
BspCNI CTCAG 1 cut(s) 773
BspLI GGNNCC 3 cut(s) 157, 650, 749
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 723
BsrI ACTGG 2 cut(s) 199, 659
BssAI RCCGGY 1 cut(s) 723
BssECI CCNNGG 2 cut(s) 274, 457
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 5 cut(s) 164, 236, 320, 434, 505
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 34, 298
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 3 cut(s) 303, 368, 791
BstDEI CTNAG 1 cut(s) 760
BstDSI CCRYGG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 6 cut(s) 34, 114, 298, 376, 492, 735
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 2 cut(s) 346, 607
BstSCI CCNGG 2 cut(s) 456, 841
BstSNI TACGTA 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 104, 363
BsuRI GGCC 3 cut(s) 5, 462, 573
BtgI CCRYGG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 3 cut(s) 439, 447, 652
Cac8I GCNNGC 3 cut(s) 303, 368, 791
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 723
Cfr13I GGNCC 1 cut(s) 460
Csp6I GTAC 9 cut(s) 202, 237, 254, 340, 346, 388, 499, 699, 866
CviAII CATG 7 cut(s) 34, 275, 343, 604, 730, 813, 861
CviQI GTAC 9 cut(s) 202, 237, 254, 340, 346, 388, 499, 699, 866
DdeI CTNAG 1 cut(s) 760
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 571
Eco105I TACGTA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 7 cut(s) 37, 278, 346, 607, 733, 816, 864
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 510, 542
FaqI GGGAC 1 cut(s) 113
FatI CATG 7 cut(s) 33, 274, 342, 603, 729, 812, 860
FauI CCCGC 2 cut(s) 90, 488
Fnu4HI GCNGC 3 cut(s) 86, 118, 377
Fsp4HI GCNGC 3 cut(s) 86, 118, 377
FspBI CTAG 4 cut(s) 312, 588, 677, 794
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 86, 118, 377
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 3 cut(s) 5, 462, 573
HapII CCGG 2 cut(s) 724, 843
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 7 cut(s) 37, 278, 346, 607, 733, 816, 864
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 1 cut(s) 548
HpaII CCGG 2 cut(s) 724, 843
HphI GGTGA 6 cut(s) 134, 210, 235, 290, 296, 460
Hpy166II GTNNAC 4 cut(s) 204, 390, 501, 593
Hpy188I TCNGA 4 cut(s) 173, 641, 763, 801
Hpy188III TCNNGA 3 cut(s) 476, 518, 708
Hpy8I GTNNAC 4 cut(s) 204, 390, 501, 593
HpyAV CCTTC 2 cut(s) 250, 323
HpyCH4III ACNGT 5 cut(s) 164, 236, 320, 434, 505
HpyCH4IV ACGT 2 cut(s) 126, 256
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 114, 298, 376, 492, 735
HpyF3I CTNAG 1 cut(s) 760
HpySE526I ACGT 2 cut(s) 126, 256
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 7 cut(s) 37, 278, 346, 607, 733, 816, 864
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 472
Lsp1109I GCAGC 2 cut(s) 104, 363
LweI GCATC 2 cut(s) 46, 511
MaeI CTAG 4 cut(s) 312, 588, 677, 794
MaeII ACGT 2 cut(s) 126, 256
MaeIII GTNAC 6 cut(s) 122, 164, 194, 428, 452, 713
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 2 cut(s) 406, 491
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 3 cut(s) 103, 132, 835
MluNI TGGCCA 1 cut(s) 573
MmeI TCCRAC 2 cut(s) 196, 255
MnlI CCTC 7 cut(s) 15, 99, 179, 274, 390, 704, 767
Mox20I TGGCCA 1 cut(s) 573
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 410
MscI TGGCCA 1 cut(s) 573
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 2 cut(s) 608, 855
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 724, 843
MspR9I CCNGG 2 cut(s) 458, 843
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 6 cut(s) 34, 114, 298, 376, 492, 735
NciI CCSGG 1 cut(s) 843
NcoI CCATGG 1 cut(s) 274
NdeII GATC 1 cut(s) 472
NlaIII CATG 7 cut(s) 37, 278, 346, 607, 733, 816, 864
NlaIV GGNNCC 3 cut(s) 157, 650, 749
NmuCI GTSAC 2 cut(s) 122, 194
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 2 cut(s) 346, 607
OliI CACNNNNGTG 1 cut(s) 855
PciI ACATGT 2 cut(s) 342, 603
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 3 cut(s) 87, 119, 378
Ple19I CGATCG 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 257
PscI ACATGT 2 cut(s) 342, 603
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 3 cut(s) 157, 650, 749
PspPI GGNCC 1 cut(s) 460
PvuI CGATCG 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 9 cut(s) 203, 238, 255, 341, 347, 389, 500, 700, 867
RsaNI GTAC 9 cut(s) 202, 237, 254, 340, 346, 388, 499, 699, 866
RseI CAYNNNNRTG 2 cut(s) 608, 855
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 3 cut(s) 86, 118, 377
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 1 cut(s) 460
ScrFI CCNGG 2 cut(s) 458, 843
SfaNI GCATC 2 cut(s) 46, 511
SmiMI CAYNNNNRTG 2 cut(s) 608, 855
SmlI CTYRAG 3 cut(s) 75, 90, 371
SmoI CTYRAG 3 cut(s) 75, 90, 371
SnaBI TACGTA 1 cut(s) 257
Sse9I AATT 3 cut(s) 103, 132, 835
SsiI CCGC 5 cut(s) 83, 86, 120, 333, 495
SspMI CTAG 4 cut(s) 312, 588, 677, 794
StyD4I CCNGG 2 cut(s) 456, 841
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 5 cut(s) 164, 236, 320, 434, 505
TaiI ACGT 2 cut(s) 129, 259
TaqI TCGA 2 cut(s) 475, 806
TasI AATT 3 cut(s) 103, 132, 835
TatI WGTACW 1 cut(s) 201
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 3 cut(s) 439, 447, 659
TseFI GTSAC 2 cut(s) 122, 194
TseI GCWGC 2 cut(s) 117, 376
Tsp45I GTSAC 2 cut(s) 122, 194
TspDTI ATGAA 1 cut(s) 693
TspRI CASTG 3 cut(s) 439, 447, 659
XapI RAATTY 1 cut(s) 835
XceI RCATGY 2 cut(s) 346, 607
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 4 cut(s) 312, 588, 677, 794
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.