RchiOBHm_Chr5g0016801

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014841
Physical Location & Seq
Reverse (-)
11542793 .. 11543206
414 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29716

Sequence Viewer

Length: 414 bp
ATGGCCTTATTACCCGCTGAGAGGTTGCAAATCATGCATCTCTTGATCTCTTATGTAGTTGTTCTATTAGTAGCAAAAGCTCAAGCCCCGCCGCCTCAGGCCAAGCCGAATTGCCCAGAGCGCTGCGGTGACGTCCCAATTCCATATCCATTTGGCATTGGTGCCGACTGTTACCTCCGACCAGAGTTCAACATCACTTGTGACCAGTACACCACACCACCATCACTAAAGTTCTCCAACAGTACCTTCACCTTTCCCGTACGTATTACTGACTTTTCCCATGGTGAGGATGAGTTGCAAGTAATACATGCTGTAGCAAGAGAGTGTTATGACGAAGGCGGTTCGTACATGCATGTACGATATGTCCAGCAACAGCACGCTCGAGCTGCCTCCTTCTTACACTATATCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

137

Amino Acids

15.45

Weight (kDa)

6.02

Isoelectric Point (pI)

66.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 101 3.6e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 412
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 5 cut(s) 15, 89, 92, 126, 339
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 5 cut(s) 209, 244, 261, 347, 357
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 2 cut(s) 21, 286
AgsI TTSAA 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 2 cut(s) 80, 386
AluI AGCT 2 cut(s) 80, 386
Ama87I CYCGRG 1 cut(s) 381
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 2 cut(s) 3, 99
ApeKI GCWGC 2 cut(s) 123, 386
AspLEI GCGC 1 cut(s) 123
AsuHPI GGTGA 3 cut(s) 140, 241, 296
AvaI CYCGRG 1 cut(s) 381
AxyI CCTNAGG 1 cut(s) 96
BanI GGYRCC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 110, 373
BccI CCATC 1 cut(s) 229
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 124
BisI GCNGC 3 cut(s) 92, 124, 387
BlsI GCNGC 3 cut(s) 93, 125, 388
BmeT110I CYCGRG 1 cut(s) 381
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 280
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 286
Bse1I ACTGG 1 cut(s) 205
Bse21I CCTNAGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 2 cut(s) 21, 286
BseMII CTCAG 2 cut(s) 9, 110
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 110, 373
BshFI GGCC 2 cut(s) 5, 101
BshNI GGYRCC 1 cut(s) 161
BsiHKCI CYCGRG 1 cut(s) 381
BsiWI CGTACG 1 cut(s) 259
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 21, 286
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 2 cut(s) 5, 101
BsoBI CYCGRG 1 cut(s) 381
Bsp143I GATC 1 cut(s) 45
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 5 cut(s) 15, 89, 92, 126, 339
BspANI GGCC 2 cut(s) 5, 101
BspCNI CTCAG 2 cut(s) 10, 109
BspLI GGNNCC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 280
Bst4CI ACNGT 2 cut(s) 170, 242
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 2 cut(s) 18, 96
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 295
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 34, 120, 386
BstNSI RCATGY 3 cut(s) 311, 352, 356
BstSFI CTRYAG 1 cut(s) 312
BstSNI TACGTA 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 110, 373
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 5, 101
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 123
Csp6I GTAC 5 cut(s) 208, 243, 260, 346, 356
CviAII CATG 5 cut(s) 34, 281, 308, 349, 353
CviJI RGCY 6 cut(s) 5, 80, 86, 101, 106, 386
CviKI_1 RGCY 6 cut(s) 5, 80, 86, 101, 106, 386
CviQI GTAC 5 cut(s) 208, 243, 260, 346, 356
DdeI CTNAG 2 cut(s) 18, 96
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
Eco105I TACGTA 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 280
Eco47III AGCGCT 1 cut(s) 122
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 280
EcoT22I ATGCAT 2 cut(s) 39, 354
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 5 cut(s) 37, 284, 311, 352, 356
FaqI GGGAC 1 cut(s) 119
FatI CATG 5 cut(s) 33, 280, 307, 348, 352
FauI CCCGC 2 cut(s) 22, 96
Fnu4HI GCNGC 3 cut(s) 92, 124, 387
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 3 cut(s) 92, 124, 387
GlaI GCGC 1 cut(s) 122
GluI GCNGC 3 cut(s) 92, 124, 387
HaeII RGCGCY 1 cut(s) 124
HaeIII GGCC 2 cut(s) 5, 101
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 5 cut(s) 37, 284, 311, 352, 356
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HphI GGTGA 3 cut(s) 140, 241, 296
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 3 cut(s) 256, 329, 403
HpyCH4III ACNGT 2 cut(s) 170, 242
HpyCH4IV ACGT 2 cut(s) 132, 262
HpyCH4V TGCA 4 cut(s) 28, 37, 298, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 120, 386
HpyF3I CTNAG 2 cut(s) 18, 96
HpySE526I ACGT 2 cut(s) 132, 262
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 5 cut(s) 37, 284, 311, 352, 356
HspAI GCGC 1 cut(s) 121
Kzo9I GATC 1 cut(s) 45
LpnPI CCDG 5 cut(s) 83, 129, 195, 218, 380
Lsp1109I GCAGC 2 cut(s) 110, 373
LweI GCATC 1 cut(s) 46
MaeII ACGT 2 cut(s) 132, 262
MaeIII GTNAC 3 cut(s) 128, 170, 200
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MluCI AATT 2 cut(s) 109, 138
MmeI TCCRAC 2 cut(s) 202, 261
MnlI CCTC 5 cut(s) 15, 105, 185, 280, 400
Mph1103I ATGCAT 2 cut(s) 39, 354
MspA1I CMGCKG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 34, 120, 386
NcoI CCATGG 1 cut(s) 280
NdeII GATC 1 cut(s) 45
NlaIII CATG 5 cut(s) 37, 284, 311, 352, 356
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 128, 200
NsiI ATGCAT 2 cut(s) 39, 354
NspI RCATGY 3 cut(s) 311, 352, 356
PaeR7I CTCGAG 1 cut(s) 381
Pfl23II CGTACG 1 cut(s) 259
PkrI GCNGC 3 cut(s) 93, 125, 388
Ppu21I YACGTR 1 cut(s) 263
PsiI TTATAA 1 cut(s) 412
PspLI CGTACG 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 163
PspXI VCTCGAGB 1 cut(s) 381
RsaI GTAC 5 cut(s) 209, 244, 261, 347, 357
RsaNI GTAC 5 cut(s) 208, 243, 260, 346, 356
SatI GCNGC 3 cut(s) 92, 124, 387
Sau3AI GATC 1 cut(s) 45
SetI ASST 8 cut(s) 26, 82, 135, 177, 248, 254, 265, 388
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 381
SlaI CTCGAG 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 81, 381
SmoI CTYRAG 2 cut(s) 81, 381
SnaBI TACGTA 1 cut(s) 263
Sse9I AATT 2 cut(s) 109, 138
SsiI CCGC 5 cut(s) 15, 89, 92, 126, 339
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 170, 242
TaiI ACGT 2 cut(s) 135, 265
TaqI TCGA 1 cut(s) 382
TasI AATT 2 cut(s) 109, 138
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 94
TseFI GTSAC 2 cut(s) 128, 200
TseI GCWGC 2 cut(s) 123, 386
Tsp45I GTSAC 2 cut(s) 128, 200
XceI RCATGY 3 cut(s) 311, 352, 356
XhoI CTCGAG 1 cut(s) 381
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 2 cut(s) 39, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.