Rmu_sc0008053.1_g000027

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008053.1
OG0014841
Physical Location & Seq
Forward (+)
90091 .. 90942
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008053.1_g000027.1.cds

Sequence Viewer

Length: 852 bp
atggccttattaccagctgggaggttgcaaatcatgcatctctcttatgtggttgttctattagtagcaaaagctcaagccccgccgcctcaagccaagccgaattgcccagagcgctgcggtgacgtcccaattccatatccatttggcattggtgccgactgttacctccgaccagagttcaacatcacttgtgaccagtacaccacacccccatcaccaaagttctccaacagtaccttcacctgtcccgtacgtattactgacttttcccttggtgagggtgagttgcaagtaatgcaagccgtagctagagagtattatgacgaaggcggttcgtacatgtacgatatgtatagcaacagcacgctcaagctgcctctttcttacactatatcttataagaacaacttcttcgtggttggttgtaacagtgtagcagtgtatcaaagttaccagggccaaatcaccgatcgagaagaaagctacaaaggcgggtacactgtgagcctgtgccaagatgctcttggcacagcacttcccaccgattcttgcaatggtttcgggtgtggccaaaatgcaatccctagtggactgcaaaacatgtcggtggatttgtatcaacttgacagctacatcagagattggaaccactggaacatagagccagactacctgtgcagctatggcttcattgtgcaagatggcaactgtttggctccaaaaccacttttgggctcaaacagcaacttcacattctctgggaacacaagttttgaagaactgaacaccacaaggagcagcttccggtggttgttgattgggcaattggcgatgagacatgtgacgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

283

Amino Acids

31.45

Weight (kDa)

4.84

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AatII GACGTC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 155
AciI CCGC 5 cut(s) 83, 86, 120, 333, 495
AcoI YGGCCR 1 cut(s) 571
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 6 cut(s) 203, 238, 255, 341, 347, 500
AfeI AGCGCT 1 cut(s) 116
AfiI CCNNNNNNNGG 2 cut(s) 280, 734
AflIII ACRYGT 3 cut(s) 342, 603, 841
AgsI TTSAA 2 cut(s) 184, 779
AjnI CCWGG 1 cut(s) 456
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 8 cut(s) 17, 74, 311, 376, 486, 633, 684, 804
AluI AGCT 8 cut(s) 17, 74, 311, 376, 486, 633, 684, 804
Alw26I GTCTC 1 cut(s) 832
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 3 cut(s) 3, 460, 571
ApeKI GCWGC 4 cut(s) 117, 376, 681, 801
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 6 cut(s) 134, 210, 235, 290, 296, 460
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 740
BbvI GCAGC 4 cut(s) 104, 363, 693, 813
BccI CCATC 2 cut(s) 223, 698
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 832
BfaI CTAG 2 cut(s) 312, 588
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 5 cut(s) 86, 118, 377, 682, 802
BlsI GCNGC 5 cut(s) 87, 119, 378, 683, 803
Bme1390I CCNGG 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 157, 650, 720
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 46, 511
BpuEI CTTGAG 3 cut(s) 60, 75, 356
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 274, 457
BsaWI WCCGGW 1 cut(s) 807
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 734
Bse1I ACTGG 2 cut(s) 199, 659
Bse3DI GCAATG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 274, 457
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 2 cut(s) 280, 734
BseMI GCAATG 1 cut(s) 562
BseNI ACTGG 2 cut(s) 199, 659
BseXI GCAGC 4 cut(s) 104, 363, 693, 813
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 700
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 3 cut(s) 5, 462, 573
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 475
BsiSI CCGG 1 cut(s) 808
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 2 cut(s) 113, 234
BslI CCNNNNNNNGG 2 cut(s) 280, 734
BsmAI GTCTC 1 cut(s) 832
BsmFI GGGAC 2 cut(s) 113, 234
BsnI GGCC 3 cut(s) 5, 462, 573
Bsp1286I GDGCHC 1 cut(s) 740
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 5 cut(s) 83, 86, 120, 333, 495
BspANI GGCC 3 cut(s) 5, 462, 573
BspLI GGNNCC 3 cut(s) 157, 650, 720
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 562
BsrI ACTGG 2 cut(s) 199, 659
BssECI CCNNGG 2 cut(s) 274, 457
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 5 cut(s) 164, 236, 434, 505, 713
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 34, 298
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 303, 368
BstENI CCTNNNNNAGG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 1 cut(s) 832
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 7 cut(s) 34, 114, 298, 376, 492, 687, 744
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 3 cut(s) 346, 607, 845
BstSCI CCNGG 1 cut(s) 456
BstSNI TACGTA 1 cut(s) 257
BstV1I GCAGC 4 cut(s) 104, 363, 693, 813
BsuRI GGCC 3 cut(s) 5, 462, 573
BtgZI GCGATG 1 cut(s) 848
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 4 cut(s) 439, 447, 501, 652
Cac8I GCNNGC 2 cut(s) 303, 368
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 460
Csp6I GTAC 6 cut(s) 202, 237, 254, 340, 346, 499
CviAII CATG 4 cut(s) 34, 343, 604, 842
CviQI GTAC 6 cut(s) 202, 237, 254, 340, 346, 499
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 571
Eco105I TACGTA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 740
Eco47III AGCGCT 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 4 cut(s) 37, 346, 607, 845
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 510, 542
FaqI GGGAC 2 cut(s) 113, 234
FatI CATG 4 cut(s) 33, 342, 603, 841
FauI CCCGC 2 cut(s) 90, 488
Fnu4HI GCNGC 5 cut(s) 86, 118, 377, 682, 802
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 5 cut(s) 86, 118, 377, 682, 802
FspBI CTAG 2 cut(s) 312, 588
GlaI GCGC 1 cut(s) 116
GluI GCNGC 5 cut(s) 86, 118, 377, 682, 802
GsaI CCCAGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 3 cut(s) 5, 462, 573
HapII CCGG 1 cut(s) 808
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 37, 346, 607, 845
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 1 cut(s) 548
HpaII CCGG 1 cut(s) 808
HphI GGTGA 6 cut(s) 134, 210, 235, 290, 296, 460
Hpy166II GTNNAC 3 cut(s) 204, 501, 593
Hpy188I TCNGA 2 cut(s) 173, 641
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 3 cut(s) 204, 501, 593
HpyAV CCTTC 2 cut(s) 250, 323
HpyCH4III ACNGT 5 cut(s) 164, 236, 434, 505, 713
HpyCH4IV ACGT 3 cut(s) 126, 256, 848
HpyCH4V TGCA 9 cut(s) 28, 37, 292, 301, 555, 581, 598, 681, 700
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 114, 298, 376, 492, 687, 744
HpySE526I ACGT 3 cut(s) 126, 256, 848
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 37, 346, 607, 845
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 2 cut(s) 724, 798
Lsp1109I GCAGC 4 cut(s) 104, 363, 693, 813
LweI GCATC 2 cut(s) 46, 511
MaeI CTAG 2 cut(s) 312, 588
MaeII ACGT 3 cut(s) 126, 256, 848
MaeIII GTNAC 6 cut(s) 122, 164, 194, 428, 452, 844
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 3 cut(s) 406, 491, 791
MfeI CAATTG 1 cut(s) 827
MhlI GDGCHC 1 cut(s) 740
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 3 cut(s) 103, 132, 827
MluNI TGGCCA 1 cut(s) 573
MmeI TCCRAC 2 cut(s) 196, 255
MnlI CCTC 5 cut(s) 15, 99, 179, 274, 390
Mox20I TGGCCA 1 cut(s) 573
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 410
MscI TGGCCA 1 cut(s) 573
MslI CAYNNNNRTG 1 cut(s) 608
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 808
MspR9I CCNGG 1 cut(s) 458
MunI CAATTG 1 cut(s) 827
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 7 cut(s) 34, 114, 298, 376, 492, 687, 744
NdeII GATC 1 cut(s) 472
NlaIII CATG 4 cut(s) 37, 346, 607, 845
NlaIV GGNNCC 3 cut(s) 157, 650, 720
NmuCI GTSAC 3 cut(s) 122, 194, 844
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 3 cut(s) 346, 607, 845
PciI ACATGT 3 cut(s) 342, 603, 841
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 548
Pfl23II CGTACG 1 cut(s) 253
PkrI GCNGC 5 cut(s) 87, 119, 378, 683, 803
Ple19I CGATCG 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 257
PscI ACATGT 3 cut(s) 342, 603, 841
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 456
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 157, 650, 720
PspPI GGNCC 1 cut(s) 460
PvuI CGATCG 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 6 cut(s) 203, 238, 255, 341, 347, 500
RsaNI GTAC 6 cut(s) 202, 237, 254, 340, 346, 499
RseI CAYNNNNRTG 1 cut(s) 608
SatI GCNGC 5 cut(s) 86, 118, 377, 682, 802
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 1 cut(s) 460
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 2 cut(s) 46, 511
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 3 cut(s) 75, 90, 371
SmoI CTYRAG 3 cut(s) 75, 90, 371
SnaBI TACGTA 1 cut(s) 257
Sse9I AATT 3 cut(s) 103, 132, 827
SsiI CCGC 5 cut(s) 83, 86, 120, 333, 495
SspMI CTAG 2 cut(s) 312, 588
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 5 cut(s) 164, 236, 434, 505, 713
TaiI ACGT 3 cut(s) 129, 259, 851
TaqI TCGA 1 cut(s) 475
TasI AATT 3 cut(s) 103, 132, 827
TatI WGTACW 1 cut(s) 201
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 1 cut(s) 548
TscAI CASTG 4 cut(s) 439, 447, 508, 659
TseFI GTSAC 3 cut(s) 122, 194, 844
TseI GCWGC 4 cut(s) 117, 376, 681, 801
Tsp45I GTSAC 3 cut(s) 122, 194, 844
TspDTI ATGAA 1 cut(s) 682
TspRI CASTG 4 cut(s) 439, 447, 508, 659
XagI CCTNNNNNAGG 1 cut(s) 278
XceI RCATGY 3 cut(s) 346, 607, 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 524
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 2 cut(s) 312, 588
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.