RLG00000023755

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014841
Physical Location & Seq
Forward (+)
27838314 .. 27840752
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023755

Sequence Viewer

Length: 2028 bp
ATGGCCTTATTACCAGCTGGGAGGTTGCAGATTATGCATCTCTCTTTTTTGGTTGTACTAGCAGCAGCTCAAACCCAGACTCCTCAAGTCAAGCCGAATTGCCCAGAGCGCTGCGGTGATGTCCAAATTTCATATCCTTTTGGCATTGGTGCCGACTGTTACCTCCGACCAGAGTTCAACATCACTTGTGACCAGCACATACCAAAGTTCTCCAACAGTACATTCACCTTTCCCGTACGTATTACGAACTTTTCCCATGGTGAGGGTGAGTTGCAAGTAATACAAGACGTAGCCAAAGTGTGTTATAACACATTCGGTTTGTCCATGGACGATATGTCCGCCAACCGCACGCTCGAGCTGCCACCTCCATACACAATCTCTCATAAGAACAACTTCTTCATAGTGGGGTGTGACACTGTAGCAGTGTATCAAGGTTTCCAAAGCAAAGTCCCAGATCCAGAAAAAAGCTACAAAGGCGGGTACACCGTGAGCCTGTGTCTAGATGCTCTTGGCACAGCACTTCCCACCAATTCTTGCAATGGTTTTGGGTGTGGCCAAAATGCGATTCCTAGTGGACTGCAAAACATGTCGGTGGAATTGTACAGCCCCTTGAGAATTTTGAACCGCTGGAACCAAGAGCCAGACTATCTGTGCAGCTATGGCTTCATTGTGCAAGATGGCAACTTCACATTCTCTGGGAACACAAGTTTTGAAGAACTGAACACCACAAGGAGCCAGCTTCCGGTGGTTGTTAATTGGGCAATTGGGGATGAGACATGTGACGTAGCAAAGAAGAAGACAGAGACTTATTCATGCAAGGAACATAGCGAATGTGTCGATCATACCATCAATGGTACTTCATCTGGAGGTTACACTTGCCGGTGCACGACAGGCTATGATGGGAACCCATACCTCTCAGATGGTTGCCAAGATATTAATGAGTGCCTGCTAGATCCGAACCCATGCAGCAAAGGAAAGTGCATAAATTTACCCGGAACTCACACTTGTACATGTGATAGTGGGTACAGATTAAATGCAAACAAGACCACTTGCGTGGAAGGAGGCAAAGGAAAGAGCACTGTCTTGAAAGTTTCATTAGGTATAAGTATAAGCATGTTTGTCTTACTGGTTGGAATTTTTTGGTTATATTGTGGAATGAAGAGAAGGAAGTTCAAACAACTGAAAGAAATCTACTTCAAAGAAAATGGTGGCGAGAAGTTACGAGAAAAACTGGCTAGTCACAAGGGATCTGTCGAGACAGCAGCCAGGATTTTTACCTCGGCAGAACTCAAGCAGGCCACAAACAATTACCACAGAGATAATATCCTTGGTGAAGGAGCTTATGGAACAGTTTACAAAGGAGTACTTGCGGATAATAAAGTGGTAGCCATAAAGAAGTCCAAAATCATGGCCAAGTCCCAGACTGATCAGTTTGTTAATGAGGTGGTTGTTCTTTCTCAAATAAACCATAGAAATGTGGTGAAGCTTTTAGGTTGCTGTTTAGAGACAGAAATGCCTTTACTAGTTTATGAGTACGTTAGCAATGGCACTCTTTATGACCACGTTCATAAAAGAAATGGAAAAGGCCCACTTTCAGTAGGATTGCGATTGAAGATTGCAGCAGAAACAGCAGAATCACTTGCGTACTTGCACTACTCCACATCAATGCAAATCGTGCACCGAGATGTGAAAGCAACTAATATACTACCGGACGAGAAATTCACGGCCAAAGTATCGGACTTTGGAGCTTCAAAATTGGTTCCTGAAGATCAAGATCAACTGTCAACTTTAGTGCAAGGAACAATGGGATACTTGGACCCTGAATATCTTCAATCAAACACACTCACAGAAAGGAGTGACGTCTATAGTTTTGGTGTTGTACTTGTGGAGCTACTAACAAGCAGAAGGGCACTTTCATTAGACAAGCCAGAAGCGGAGCGAAGCCTAGCAAACGTCTTTGTTTGTGCAGTGGAAGATGGTCTATTGAAGCACATACTTGATGAGGAAGTAGTTAAGGACGGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

676

Amino Acids

74.45

Weight (kDa)

6.18

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 93 8e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 311 - 351 1.5e-08 Calcium-binding EGF domain
FXa_inhibition PF14670 315 - 351 8.2e-06 Coagulation Factor Xa inhibitory site
Pkinase PF00069 437 - 642 1.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 440 - 634 2.3e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AatII GACGTC 1 cut(s) 1863
AccB1I GGYRCC 1 cut(s) 149
AciI CCGC 7 cut(s) 114, 339, 346, 477, 625, 1370, 1934
AclWI GGATC 3 cut(s) 449, 947, 1255
AcoI YGGCCR 3 cut(s) 553, 1410, 1725
AcsI RAATTY 5 cut(s) 126, 615, 985, 1134, 1718
AcuI CTGAAG 1 cut(s) 1785
AcyI GRCGYC 1 cut(s) 1860
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 262, 742
AflIII ACRYGT 3 cut(s) 585, 776, 1010
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 1522
AjnI CCWGG 1 cut(s) 1265
AjuI GAANNNNNNNTTGG 4 cut(s) 206, 238, 549, 581
AleI CACNNNNGTG 1 cut(s) 1052
Alw21I GWGCWC 3 cut(s) 887, 1079, 1680
Alw26I GTCTC 4 cut(s) 767, 797, 1250, 1499
Alw44I GTGCAC 2 cut(s) 883, 1676
AlwI GGATC 3 cut(s) 449, 947, 1255
Ama87I CYCGRG 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 6 cut(s) 3, 553, 1296, 1410, 1585, 1725
ApaLI GTGCAC 2 cut(s) 883, 1676
ApeKI GCWGC 8 cut(s) 62, 65, 111, 358, 654, 966, 1262, 1619
ApoI RAATTY 5 cut(s) 126, 615, 985, 1134, 1718
AseI ATTAAT 1 cut(s) 936
Asp700I GAANNNNTTC 2 cut(s) 392, 1827
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 1586, 1816
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 6 cut(s) 128, 217, 272, 278, 1343, 1492
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 1 cut(s) 1816
BaeGI GKGCMC 3 cut(s) 887, 1680, 1912
BalI TGGCCA 2 cut(s) 555, 1412
BanI GGYRCC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 3 cut(s) 887, 1079, 1680
BbvI GCAGC 8 cut(s) 74, 77, 98, 345, 666, 978, 1274, 1631
BccI CCATC 5 cut(s) 671, 854, 893, 914, 1970
BceAI ACGGC 1 cut(s) 1740
BciT130I CCWGG 1 cut(s) 1267
BciVI GTATCC 1 cut(s) 1802
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 4 cut(s) 767, 797, 1250, 1499
BcuI ACTAGT 1 cut(s) 1522
BfaI CTAG 7 cut(s) 59, 500, 570, 950, 1236, 1523, 1946
BfmI CTRYAG 2 cut(s) 417, 1864
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 1802
BisI GCNGC 8 cut(s) 63, 66, 112, 359, 655, 967, 1263, 1620
BlsI GCNGC 8 cut(s) 64, 67, 113, 360, 656, 968, 1264, 1621
BmcAI AGTACT 1 cut(s) 1365
Bme1390I CCNGG 2 cut(s) 993, 1267
Bme18I GGWCC 1 cut(s) 1816
BmeRI GACNNNNNGTC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 2 cut(s) 1586, 1816
BmiI GGNNCC 6 cut(s) 151, 632, 734, 905, 1761, 1818
BmrFI CCNGG 2 cut(s) 993, 1267
BmsI GCATC 2 cut(s) 46, 493
BpiI GAAGAC 1 cut(s) 803
BpmI CTGGAG 1 cut(s) 885
BpuEI CTTGAG 3 cut(s) 69, 631, 1274
BpuMI CCSGG 1 cut(s) 993
BsaAI YACGTR 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 1773
BsaHI GRCGYC 1 cut(s) 1860
BsaJI CCNNGG 4 cut(s) 256, 324, 1278, 1327
BsaWI WCCGGW 2 cut(s) 742, 1708
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 742
Bse118I RCCGGY 1 cut(s) 879
Bse1I ACTGG 2 cut(s) 1131, 1236
Bse3DI GCAATG 2 cut(s) 544, 1549
Bse8I GATNNNNATC 1 cut(s) 1773
BseBI CCWGG 1 cut(s) 1267
BseDI CCNNGG 4 cut(s) 256, 324, 1278, 1327
BseGI GGATG 1 cut(s) 775
BseJI GATNNNNATC 1 cut(s) 1773
BseLI CCNNNNNNNGG 2 cut(s) 262, 742
BseMI GCAATG 2 cut(s) 544, 1549
BseMII CTCAG 1 cut(s) 930
BseNI ACTGG 2 cut(s) 1131, 1236
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 3 cut(s) 887, 1680, 1912
BseXI GCAGC 8 cut(s) 74, 77, 98, 345, 666, 978, 1274, 1631
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 2 cut(s) 673, 1986
BshFI GGCC 6 cut(s) 5, 555, 1298, 1412, 1587, 1727
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 3 cut(s) 887, 1079, 1680
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 743, 880, 993, 1709
BsiWI CGTACG 1 cut(s) 235
BslFI GGGAC 2 cut(s) 434, 1402
BslI CCNNNNNNNGG 2 cut(s) 262, 742
BsmAI GTCTC 4 cut(s) 767, 797, 1250, 1499
BsmFI GGGAC 2 cut(s) 434, 1402
BsnI GGCC 6 cut(s) 5, 555, 1298, 1412, 1587, 1727
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 4 cut(s) 887, 1079, 1680, 1912
Bsp1407I TGTACA 2 cut(s) 600, 1007
Bsp143I GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
Bsp19I CCATGG 2 cut(s) 256, 324
BspACI CCGC 7 cut(s) 114, 339, 346, 477, 625, 1370, 1934
BspANI GGCC 6 cut(s) 5, 555, 1298, 1412, 1587, 1727
BspCNI CTCAG 1 cut(s) 929
BspLI GGNNCC 6 cut(s) 151, 632, 734, 905, 1761, 1818
BspPI GGATC 3 cut(s) 449, 947, 1255
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 544, 1549
BsrFI RCCGGY 1 cut(s) 879
BsrGI TGTACA 2 cut(s) 600, 1007
BsrI ACTGG 2 cut(s) 1131, 1236
BssAI RCCGGY 1 cut(s) 879
BssECI CCNNGG 4 cut(s) 256, 324, 1278, 1327
BssMI GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
BssNI GRCGYC 1 cut(s) 1860
BssT1I CCWWGG 3 cut(s) 256, 324, 1327
Bst2UI CCWGG 1 cut(s) 1267
Bst4CI ACNGT 7 cut(s) 158, 218, 418, 487, 1081, 1351, 1782
Bst6I CTCTTC 1 cut(s) 1154
BstACI GRCGYC 1 cut(s) 1860
BstAPI GCANNNNNTGC 2 cut(s) 34, 1675
BstAUI TGTACA 2 cut(s) 600, 1007
BstBAI YACGTR 1 cut(s) 239
BstC8I GCNNGC 4 cut(s) 350, 737, 947, 1296
BstDEI CTNAG 1 cut(s) 916
BstDSI CCRYGG 2 cut(s) 256, 324
BstF5I GGATG 1 cut(s) 775
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 7 cut(s) 457, 841, 955, 1250, 1429, 1771, 1777
BstMAI GTCTC 4 cut(s) 767, 797, 1250, 1499
BstMBI GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
BstMWI GCNNNNNNNGC 8 cut(s) 34, 108, 358, 474, 660, 891, 1628, 1675
BstNI CCWGG 1 cut(s) 1267
BstNSI RCATGY 4 cut(s) 589, 780, 1014, 1117
BstSCI CCNGG 2 cut(s) 991, 1265
BstSFI CTRYAG 2 cut(s) 417, 1864
BstSLI GKGCMC 3 cut(s) 887, 1680, 1912
BstSNI TACGTA 1 cut(s) 239
BstV1I GCAGC 8 cut(s) 74, 77, 98, 345, 666, 978, 1274, 1631
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 3 cut(s) 454, 952, 1247
BstXI CCANNNNNNTGG 2 cut(s) 1054, 1408
BstYI RGATCY 3 cut(s) 454, 952, 1247
BsuI GTATCC 1 cut(s) 1802
BsuRI GGCC 6 cut(s) 5, 555, 1298, 1412, 1587, 1727
BtgI CCRYGG 2 cut(s) 256, 324
BtsCI GGATG 1 cut(s) 775
BtsI GCAGTG 2 cut(s) 429, 1974
BtsIMutI CAGTG 4 cut(s) 414, 429, 1077, 1974
Cac8I GCNNGC 4 cut(s) 350, 737, 947, 1296
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 879
Cfr13I GGNCC 2 cut(s) 1586, 1816
CviAII CATG 9 cut(s) 257, 325, 586, 777, 813, 963, 1011, 1114, 1408
DdeI CTNAG 1 cut(s) 916
DpnI GATC 7 cut(s) 456, 840, 954, 1249, 1428, 1770, 1776
DpnII GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 3 cut(s) 553, 1410, 1725
Eam1104I CTCTTC 1 cut(s) 1154
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 1154
EciI GGCGGA 1 cut(s) 328
Eco105I TACGTA 1 cut(s) 239
Eco130I CCWWGG 3 cut(s) 256, 324, 1327
Eco47I GGWCC 1 cut(s) 1816
Eco47III AGCGCT 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 1785
Eco88I CYCGRG 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 1265
EcoT14I CCWWGG 3 cut(s) 256, 324, 1327
EcoT22I ATGCAT 1 cut(s) 39
ErhI CCWWGG 3 cut(s) 256, 324, 1327
FaeI CATG 9 cut(s) 260, 328, 589, 780, 816, 966, 1014, 1117, 1411
FalI AAGNNNNNCTT 6 cut(s) 377, 409, 1350, 1382, 1575, 1607
FaqI GGGAC 2 cut(s) 434, 1402
FatI CATG 9 cut(s) 256, 324, 585, 776, 812, 962, 1010, 1113, 1407
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 1426
Fnu4HI GCNGC 8 cut(s) 63, 66, 112, 359, 655, 967, 1263, 1620
FokI GGATG 1 cut(s) 782
Fsp4HI GCNGC 8 cut(s) 63, 66, 112, 359, 655, 967, 1263, 1620
FspBI CTAG 7 cut(s) 59, 500, 570, 950, 1236, 1523, 1946
GlaI GCGC 1 cut(s) 110
GluI GCNGC 8 cut(s) 63, 66, 112, 359, 655, 967, 1263, 1620
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 885
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 6 cut(s) 5, 555, 1298, 1412, 1587, 1727
HapII CCGG 4 cut(s) 743, 880, 993, 1709
HhaI GCGC 1 cut(s) 111
Hin1I GRCGYC 1 cut(s) 1860
Hin1II CATG 9 cut(s) 260, 328, 589, 780, 816, 966, 1014, 1117, 1411
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 1785
HindII GTYRAC 1 cut(s) 1785
HindIII AAGCTT 1 cut(s) 1484
HinfI GANTC 3 cut(s) 79, 565, 1634
HpaII CCGG 4 cut(s) 743, 880, 993, 1709
HphI GGTGA 6 cut(s) 128, 217, 272, 278, 1343, 1492
Hpy166II GTNNAC 6 cut(s) 483, 575, 885, 1354, 1678, 1785
Hpy188I TCNGA 4 cut(s) 167, 919, 957, 1738
Hpy188III TCNNGA 7 cut(s) 458, 500, 864, 1084, 1255, 1763, 1772
Hpy8I GTNNAC 6 cut(s) 483, 575, 885, 1354, 1678, 1785
HpyAV CCTTC 4 cut(s) 1052, 1158, 1328, 1899
HpyCH4III ACNGT 7 cut(s) 158, 218, 418, 487, 1081, 1351, 1782
HpyCH4IV ACGT 7 cut(s) 238, 288, 783, 1536, 1563, 1860, 1953
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 108, 358, 474, 660, 891, 1628, 1675
HpyF3I CTNAG 1 cut(s) 916
HpySE526I ACGT 7 cut(s) 238, 288, 783, 1536, 1563, 1860, 1953
Hsp92I GRCGYC 1 cut(s) 1860
Hsp92II CATG 9 cut(s) 260, 328, 589, 780, 816, 966, 1014, 1117, 1411
HspAI GCGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 1426
Kzo9I GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
LmnI GCTCC 5 cut(s) 732, 1337, 1745, 1888, 1936
Lsp1109I GCAGC 8 cut(s) 74, 77, 98, 345, 666, 978, 1274, 1631
LweI GCATC 2 cut(s) 46, 493
MaeI CTAG 7 cut(s) 59, 500, 570, 950, 1236, 1523, 1946
MaeII ACGT 7 cut(s) 238, 288, 783, 1536, 1563, 1860, 1953
MaeIII GTNAC 8 cut(s) 158, 188, 410, 779, 869, 1218, 1238, 1856
MalI GATC 7 cut(s) 456, 840, 954, 1249, 1428, 1770, 1776
MboI GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
MboII GAAGA 9 cut(s) 388, 725, 805, 808, 1171, 1624, 1778, 1820, 1985
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 454, 952, 1247
MhlI GDGCHC 4 cut(s) 887, 1079, 1680, 1912
MlsI TGGCCA 2 cut(s) 555, 1412
MluNI TGGCCA 2 cut(s) 555, 1412
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 3 cut(s) 190, 237, 1111
Mox20I TGGCCA 2 cut(s) 555, 1412
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 2 cut(s) 392, 1827
MscI TGGCCA 2 cut(s) 555, 1412
MseI TTAA 5 cut(s) 753, 936, 1031, 1437, 2013
MslI CAYNNNNRTG 5 cut(s) 590, 1052, 1473, 1664, 1683
Msp20I TGGCCA 2 cut(s) 555, 1412
MspA1I CMGCKG 2 cut(s) 17, 627
MspI CCGG 4 cut(s) 743, 880, 993, 1709
MspR9I CCNGG 2 cut(s) 993, 1267
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 1 cut(s) 1267
MwoI GCNNNNNNNGC 8 cut(s) 34, 108, 358, 474, 660, 891, 1628, 1675
NciI CCSGG 1 cut(s) 993
NcoI CCATGG 2 cut(s) 256, 324
NdeII GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
NlaIII CATG 9 cut(s) 260, 328, 589, 780, 816, 966, 1014, 1117, 1411
NlaIV GGNNCC 6 cut(s) 151, 632, 734, 905, 1761, 1818
NmeAIII GCCGAG 1 cut(s) 1259
NmuCI GTSAC 5 cut(s) 188, 410, 779, 1238, 1856
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 4 cut(s) 589, 780, 1014, 1117
OliI CACNNNNGTG 1 cut(s) 1052
PaeR7I CTCGAG 1 cut(s) 353
PciI ACATGT 3 cut(s) 585, 776, 1010
PdmI GAANNNNTTC 2 cut(s) 392, 1827
PfeI GAWTC 2 cut(s) 565, 1634
Pfl23II CGTACG 1 cut(s) 235
PkrI GCNGC 8 cut(s) 64, 67, 113, 360, 656, 968, 1264, 1621
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 239
PscI ACATGT 3 cut(s) 585, 776, 1010
PshBI ATTAAT 1 cut(s) 936
PsiI TTATAA 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 1265
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 1265
PspLI CGTACG 1 cut(s) 235
PspN4I GGNNCC 6 cut(s) 151, 632, 734, 905, 1761, 1818
PspPI GGNCC 2 cut(s) 1586, 1816
PspXI VCTCGAGB 1 cut(s) 353
PsuI RGATCY 3 cut(s) 454, 952, 1247
PvuII CAGCTG 1 cut(s) 17
RseI CAYNNNNRTG 5 cut(s) 590, 1052, 1473, 1664, 1683
SaqAI TTAA 5 cut(s) 753, 936, 1031, 1437, 2013
SatI GCNGC 8 cut(s) 63, 66, 112, 359, 655, 967, 1263, 1620
Sau3AI GATC 7 cut(s) 454, 838, 952, 1247, 1426, 1768, 1774
Sau96I GGNCC 2 cut(s) 1586, 1816
ScaI AGTACT 1 cut(s) 1365
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 2 cut(s) 993, 1267
SduI GDGCHC 4 cut(s) 887, 1079, 1680, 1912
SfaNI GCATC 2 cut(s) 46, 493
SfcI CTRYAG 2 cut(s) 417, 1864
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 1 cut(s) 1816
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 5 cut(s) 590, 1052, 1473, 1664, 1683
SmlI CTYRAG 4 cut(s) 84, 353, 610, 1289
SmoI CTYRAG 4 cut(s) 84, 353, 610, 1289
SnaBI TACGTA 1 cut(s) 239
SpeI ACTAGT 1 cut(s) 1522
SsiI CCGC 7 cut(s) 114, 339, 346, 477, 625, 1370, 1934
SspMI CTAG 7 cut(s) 59, 500, 570, 950, 1236, 1523, 1946
StyD4I CCNGG 2 cut(s) 991, 1265
StyI CCWWGG 3 cut(s) 256, 324, 1327
TaaI ACNGT 7 cut(s) 158, 218, 418, 487, 1081, 1351, 1782
TaiI ACGT 7 cut(s) 241, 291, 786, 1539, 1566, 1863, 1956
TaqI TCGA 3 cut(s) 354, 837, 1254
TatI WGTACW 6 cut(s) 55, 218, 600, 1007, 1363, 1879
TfiI GAWTC 2 cut(s) 565, 1634
Tru1I TTAA 5 cut(s) 753, 936, 1031, 1437, 2013
Tru9I TTAA 5 cut(s) 753, 936, 1031, 1437, 2013
TscAI CASTG 4 cut(s) 421, 429, 1084, 1974
TseFI GTSAC 5 cut(s) 188, 410, 779, 1238, 1856
TseI GCWGC 8 cut(s) 62, 65, 111, 358, 654, 966, 1262, 1619
Tsp45I GTSAC 5 cut(s) 188, 410, 779, 1238, 1856
TspDTI ATGAA 9 cut(s) 120, 388, 655, 801, 849, 1083, 1172, 1556, 1905
TspRI CASTG 4 cut(s) 421, 429, 1084, 1974
VneI GTGCAC 2 cut(s) 883, 1676
VpaK11BI GGWCC 1 cut(s) 1816
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 5 cut(s) 126, 615, 985, 1134, 1718
XbaI TCTAGA 1 cut(s) 499
XceI RCATGY 4 cut(s) 589, 780, 1014, 1117
XhoI CTCGAG 1 cut(s) 353
XmnI GAANNNNTTC 2 cut(s) 392, 1827
XspI CTAG 7 cut(s) 59, 500, 570, 950, 1236, 1523, 1946
ZraI GACGTC 1 cut(s) 1861
ZrmI AGTACT 1 cut(s) 1365
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.