RchiOBHm_Chr3g0482951

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016368
Physical Location & Seq
Forward (+)
29034520 .. 29035813
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44776

Sequence Viewer

Length: 1068 bp
ATGCACAAATTCCGGGAAGAGGAGGCAATGTACTTCAGCCCAAGGGATCGGAAGTATCCGAACGGTAAGCTTCCGAGCCGCATAACTGGTGAAGGTGATGGATTATGGAAGGCAAGTGGTAAAGACTTGGAAATTTTCAGTCAAAGGTGGAAGGTAATTGGAGCCAGGAAGACCCTGGTCTTTTATGATCTGAAATCAAAACATGGAGCAAAAAAGACCAATTGGATTATGCATGAATACACAATCGAGGAAAACATCTCACCATCCACAAGCACCACTAGAGCTCCTGGGGACAACAGGTTGGATGATTGGGTTTTGTGCAAGGTGTATCTAAGTAAGATGTCTAAATCTAAACCAAATACTGCGGATGGAGGGAAAACAGAGATGACAGATTCAGAGCCATTAGAGCAGGATCTCCCAGTGCTCGAAAGCTCTGCGACTCCAAACCTAGGTGAACAAGTTAACGTGTGCAATGAAAGTGCGCAAGCATTATACAATGGAATCATGACAGTACCATATGGCTCTACTACTGGTTATGTGTCTCAAGAGGTGACAAATTTAGGTGCAAGGTCGCTTGCTCATCATCAACAAAACCTGTCATTGCTTCCAAGCTTTTCGCAGCTTCCAGTTATTAACAGTGTTACCTCTGCTATTTCCAGCATTGCTGGAGGAGACAACAACAATGGAACGATCACTACACCAAATGATGGTTCAAATGGATCTGAAAAGGTGATAAATTTGCATAAAAACTTGCATGCAGATCATCAGCAAGACCTTCCATCACTTCAAAACTCTTCGAAGCCCTCTGTTATTAACAGCGTTGGGTCTGCTATTGCCAGTCTTGCCGGCAGTCCAGTGTTTCCTGATTTCCAACAGGTTCCTCGTGGTTTTGGAGCTCAACATGGGACAAATATAGCTCCCATAAATGGAATCCAAGCACGTCAGTCCCCTCATGGAATTGGACCTCATCAATCTCGGCCAAGTTTGAACAATGGCTGCCTCCCTCCATTACGAACTTTTGTAGGTTTCCTAGTGCTTTTAGGATTTGGTAAGGTCAGTTCCACTTAA

Protein Analysis

355

Amino Acids

38.41

Weight (kDa)

8.32

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 3 - 81 7.8e-17 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
AccB7I CCANNNNNTGG 1 cut(s) 707
AciI CCGC 2 cut(s) 79, 365
AclWI GGATC 3 cut(s) 54, 420, 727
AcoI YGGCCR 1 cut(s) 977
AcsI RAATTY 4 cut(s) 8, 132, 556, 736
AcuI CTGAAG 1 cut(s) 19
AfaI GTAC 2 cut(s) 32, 513
AfiI CCNNNNNNNGG 4 cut(s) 19, 449, 707, 926
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 3 cut(s) 714, 788, 988
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 3 cut(s) 164, 174, 286
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 7 cut(s) 70, 284, 432, 612, 622, 896, 917
AluI AGCT 7 cut(s) 70, 284, 432, 612, 622, 896, 917
Alw21I GWGCWC 3 cut(s) 286, 426, 898
Alw26I GTCTC 2 cut(s) 546, 666
AlwI GGATC 3 cut(s) 54, 420, 727
AoxI GGCC 1 cut(s) 977
ApeKI GCWGC 2 cut(s) 619, 996
ApoI RAATTY 4 cut(s) 8, 132, 556, 736
AspA2I CCTAGG 1 cut(s) 448
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 1 cut(s) 962
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 6 cut(s) 101, 107, 252, 464, 562, 742
AsuII TTCGAA 1 cut(s) 797
AvaII GGWCC 1 cut(s) 962
AvrII CCTAGG 1 cut(s) 448
BanII GRGCYC 2 cut(s) 286, 898
BauI CACGAG 1 cut(s) 882
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 3 cut(s) 286, 426, 898
BbvI GCAGC 2 cut(s) 631, 983
BccI CCATC 5 cut(s) 92, 271, 362, 701, 787
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 3 cut(s) 166, 176, 288
BciVI GTATCC 1 cut(s) 66
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 2 cut(s) 546, 666
BfaI CTAG 3 cut(s) 279, 449, 1031
BfuI GTATCC 1 cut(s) 66
BisI GCNGC 3 cut(s) 79, 620, 997
BlnI CCTAGG 1 cut(s) 448
BlsI GCNGC 3 cut(s) 80, 621, 998
Bme1390I CCNGG 4 cut(s) 14, 166, 176, 288
Bme18I GGWCC 1 cut(s) 962
BmgBI CACGTC 1 cut(s) 941
BmgT120I GGNCC 1 cut(s) 962
BmiI GGNNCC 2 cut(s) 163, 879
BmrFI CCNGG 4 cut(s) 14, 166, 176, 288
BmrI ACTGGG 1 cut(s) 413
BmuI ACTGGG 1 cut(s) 413
BoxI GACNNNNGTC 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 176
BpmI CTGGAG 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 1 cut(s) 528
BpuMI CCSGG 1 cut(s) 14
BsaJI CCNNGG 4 cut(s) 41, 174, 287, 448
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 268, 298
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 449, 707, 926
Bse118I RCCGGY 1 cut(s) 845
Bse1I ACTGG 6 cut(s) 91, 419, 535, 626, 837, 854
Bse3DI GCAATG 4 cut(s) 33, 478, 599, 660
BseBI CCWGG 3 cut(s) 166, 176, 288
BseDI CCNNGG 4 cut(s) 41, 174, 287, 448
BseGI GGATG 3 cut(s) 263, 310, 373
BseLI CCNNNNNNNGG 4 cut(s) 19, 449, 707, 926
BseMI GCAATG 4 cut(s) 33, 478, 599, 660
BseNI ACTGG 6 cut(s) 91, 419, 535, 626, 837, 854
BseRI GAGGAG 2 cut(s) 35, 684
BseXI GCAGC 2 cut(s) 631, 983
BshFI GGCC 1 cut(s) 979
BsiHKAI GWGCWC 3 cut(s) 286, 426, 898
BsiSI CCGG 2 cut(s) 13, 846
BslFI GGGAC 3 cut(s) 305, 919, 931
BslI CCNNNNNNNGG 4 cut(s) 19, 449, 707, 926
BsmAI GTCTC 2 cut(s) 546, 666
BsmFI GGGAC 3 cut(s) 305, 919, 931
BsnI GGCC 1 cut(s) 979
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 3 cut(s) 286, 426, 898
Bsp143I GATC 6 cut(s) 46, 187, 412, 690, 719, 760
BspACI CCGC 2 cut(s) 79, 365
BspANI GGCC 1 cut(s) 979
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 163, 879
BspPI GGATC 3 cut(s) 54, 420, 727
BspT104I TTCGAA 1 cut(s) 797
BsrDI GCAATG 4 cut(s) 33, 478, 599, 660
BsrFI RCCGGY 1 cut(s) 845
BsrI ACTGG 6 cut(s) 91, 419, 535, 626, 837, 854
BssAI RCCGGY 1 cut(s) 845
BssECI CCNNGG 4 cut(s) 41, 174, 287, 448
BssMI GATC 6 cut(s) 46, 187, 412, 690, 719, 760
BssSI CACGAG 1 cut(s) 882
BssT1I CCWWGG 2 cut(s) 41, 448
Bst2BI CACGAG 1 cut(s) 882
Bst2UI CCWGG 3 cut(s) 166, 176, 288
Bst4CI ACNGT 3 cut(s) 65, 511, 638
Bst6I CTCTTC 2 cut(s) 12, 799
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 486, 576, 756, 847
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 3 cut(s) 263, 310, 373
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 6 cut(s) 49, 190, 415, 693, 722, 763
BstMAI GTCTC 2 cut(s) 546, 666
BstMBI GATC 6 cut(s) 46, 187, 412, 690, 719, 760
BstMWI GCNNNNNNNGC 2 cut(s) 406, 842
BstNI CCWGG 3 cut(s) 166, 176, 288
BstNSI RCATGY 1 cut(s) 758
BstPAI GACNNNNGTC 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 12, 164, 174, 286
BstV1I GCAGC 2 cut(s) 631, 983
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 2 cut(s) 412, 719
BstYI RGATCY 2 cut(s) 412, 719
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 979
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 3 cut(s) 263, 310, 373
BtsIMutI CAGTG 3 cut(s) 426, 643, 861
Cac8I GCNNGC 4 cut(s) 486, 576, 756, 847
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 845
Cfr13I GGNCC 1 cut(s) 962
Csp6I GTAC 2 cut(s) 31, 512
CviAII CATG 6 cut(s) 203, 233, 505, 755, 902, 953
CviQI GTAC 2 cut(s) 31, 512
DdeI CTNAG 1 cut(s) 332
DpnI GATC 6 cut(s) 48, 189, 414, 692, 721, 762
DpnII GATC 6 cut(s) 46, 187, 412, 690, 719, 760
EaeI YGGCCR 1 cut(s) 977
Eam1104I CTCTTC 2 cut(s) 12, 799
EarI CTCTTC 2 cut(s) 12, 799
Ecl136II GAGCTC 2 cut(s) 284, 896
Eco130I CCWWGG 2 cut(s) 41, 448
Eco24I GRGCYC 2 cut(s) 286, 898
Eco47I GGWCC 1 cut(s) 962
Eco53kI GAGCTC 2 cut(s) 284, 896
Eco57I CTGAAG 1 cut(s) 19
EcoICRI GAGCTC 2 cut(s) 284, 896
EcoRII CCWGG 3 cut(s) 164, 174, 286
EcoT14I CCWWGG 2 cut(s) 41, 448
EcoT22I ATGCAT 1 cut(s) 234
EcoT38I GRGCYC 2 cut(s) 286, 898
ErhI CCWWGG 2 cut(s) 41, 448
FaeI CATG 6 cut(s) 206, 236, 508, 758, 905, 956
FaqI GGGAC 3 cut(s) 305, 919, 931
FatI CATG 6 cut(s) 202, 232, 504, 754, 901, 952
FauNDI CATATG 1 cut(s) 517
Fnu4HI GCNGC 3 cut(s) 79, 620, 997
FokI GGATG 3 cut(s) 250, 317, 380
FriOI GRGCYC 2 cut(s) 286, 898
Fsp4HI GCNGC 3 cut(s) 79, 620, 997
FspBI CTAG 3 cut(s) 279, 449, 1031
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 1 cut(s) 483
GluI GCNGC 3 cut(s) 79, 620, 997
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 1 cut(s) 979
HapII CCGG 2 cut(s) 13, 846
HhaI GCGC 1 cut(s) 484
Hin1II CATG 6 cut(s) 206, 236, 508, 758, 905, 956
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 2 cut(s) 68, 610
HinfI GANTC 4 cut(s) 392, 439, 501, 930
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 13, 846
HphI GGTGA 6 cut(s) 101, 107, 252, 464, 562, 742
Hpy166II GTNNAC 2 cut(s) 455, 463
Hpy188I TCNGA 6 cut(s) 51, 60, 75, 192, 397, 724
Hpy188III TCNNGA 3 cut(s) 505, 545, 863
Hpy8I GTNNAC 2 cut(s) 455, 463
HpyAV CCTTC 4 cut(s) 86, 103, 145, 785
HpyCH4III ACNGT 3 cut(s) 65, 511, 638
HpyCH4IV ACGT 2 cut(s) 465, 940
HpyCH4V TGCA 8 cut(s) 4, 232, 321, 471, 566, 742, 754, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 406, 842
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 2 cut(s) 465, 940
Hsp92II CATG 6 cut(s) 206, 236, 508, 758, 905, 956
HspAI GCGC 1 cut(s) 482
KroI GCCGGC 1 cut(s) 845
KroNI GCCGGC 1 cut(s) 847
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 6 cut(s) 46, 187, 412, 690, 719, 760
LmnI GCTCC 5 cut(s) 161, 206, 289, 893, 922
Lsp1109I GCAGC 2 cut(s) 631, 983
MaeI CTAG 3 cut(s) 279, 449, 1031
MaeII ACGT 2 cut(s) 465, 940
MaeIII GTNAC 2 cut(s) 550, 640
MalI GATC 6 cut(s) 48, 189, 414, 692, 721, 762
MboI GATC 6 cut(s) 46, 187, 412, 690, 719, 760
MboII GAAGA 3 cut(s) 29, 181, 786
MfeI CAATTG 1 cut(s) 220
MflI RGATCY 2 cut(s) 412, 719
MhlI GDGCHC 3 cut(s) 286, 426, 898
MluCI AATT 7 cut(s) 8, 132, 156, 220, 556, 736, 957
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 2 cut(s) 282, 895
Mph1103I ATGCAT 1 cut(s) 234
MroNI GCCGGC 1 cut(s) 845
MseI TTAA 4 cut(s) 462, 633, 813, 1066
MspI CCGG 2 cut(s) 13, 846
MspR9I CCNGG 4 cut(s) 14, 166, 176, 288
MunI CAATTG 1 cut(s) 220
MvaI CCWGG 3 cut(s) 166, 176, 288
MwoI GCNNNNNNNGC 2 cut(s) 406, 842
NaeI GCCGGC 1 cut(s) 847
NciI CCSGG 1 cut(s) 14
NdeI CATATG 1 cut(s) 517
NdeII GATC 6 cut(s) 46, 187, 412, 690, 719, 760
NgoMIV GCCGGC 1 cut(s) 845
NlaIII CATG 6 cut(s) 206, 236, 508, 758, 905, 956
NlaIV GGNNCC 2 cut(s) 163, 879
NmeAIII GCCGAG 1 cut(s) 955
NmuCI GTSAC 1 cut(s) 550
NsbI TGCGCA 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 234
NspI RCATGY 1 cut(s) 758
NspV TTCGAA 1 cut(s) 797
PaeI GCATGC 1 cut(s) 758
PagI TCATGA 1 cut(s) 504
PdiI GCCGGC 1 cut(s) 847
PfeI GAWTC 3 cut(s) 392, 501, 930
PflMI CCANNNNNTGG 1 cut(s) 707
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 3 cut(s) 80, 621, 998
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PshAI GACNNNNGTC 1 cut(s) 176
Psp124BI GAGCTC 2 cut(s) 286, 898
Psp6I CCWGG 3 cut(s) 164, 174, 286
PspGI CCWGG 3 cut(s) 164, 174, 286
PspN4I GGNNCC 2 cut(s) 163, 879
PspPI GGNCC 1 cut(s) 962
PsrI GAACNNNNNNTAC 3 cut(s) 679, 711, 1042
PsuI RGATCY 2 cut(s) 412, 719
RsaI GTAC 2 cut(s) 32, 513
RsaNI GTAC 2 cut(s) 31, 512
SacI GAGCTC 2 cut(s) 286, 898
SaqAI TTAA 4 cut(s) 462, 633, 813, 1066
SatI GCNGC 3 cut(s) 79, 620, 997
Sau3AI GATC 6 cut(s) 46, 187, 412, 690, 719, 760
Sau96I GGNCC 1 cut(s) 962
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 4 cut(s) 14, 166, 176, 288
SduI GDGCHC 3 cut(s) 286, 426, 898
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 1 cut(s) 962
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
SphI GCATGC 1 cut(s) 758
Sse9I AATT 7 cut(s) 8, 132, 156, 220, 556, 736, 957
SsiI CCGC 2 cut(s) 79, 365
SspMI CTAG 3 cut(s) 279, 449, 1031
SstI GAGCTC 2 cut(s) 286, 898
StyD4I CCNGG 4 cut(s) 12, 164, 174, 286
StyI CCWWGG 2 cut(s) 41, 448
TaaI ACNGT 3 cut(s) 65, 511, 638
TaiI ACGT 2 cut(s) 468, 943
TaqI TCGA 3 cut(s) 246, 426, 797
TasI AATT 7 cut(s) 8, 132, 156, 220, 556, 736, 957
TatI WGTACW 1 cut(s) 30
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 3 cut(s) 392, 501, 930
Tru1I TTAA 4 cut(s) 462, 633, 813, 1066
Tru9I TTAA 4 cut(s) 462, 633, 813, 1066
TscAI CASTG 3 cut(s) 426, 643, 861
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 2 cut(s) 619, 996
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 249, 489
TspRI CASTG 3 cut(s) 426, 643, 861
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 962
XapI RAATTY 4 cut(s) 8, 132, 556, 736
XceI RCATGY 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmaJI CCTAGG 1 cut(s) 448
XspI CTAG 3 cut(s) 279, 449, 1031
Zsp2I ATGCAT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.