RLG00000023315

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016368
Physical Location & Seq
Reverse (-)
21765699 .. 21768402
2704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023315

Sequence Viewer

Length: 1299 bp
ATGGCTGGTACTAGTAGGAACCCAAACCCTCACAATTCAGCTCCGTATGATGACATCGAGATGATGAAGCCATATGCGTTCTTTTACAACAGGTTTCCATGGGGCTTTAAGTTTCAACCTAGTGATGAAGAGTTGATTCTTCAATACTTGATGAACAAACTCTTGAATAAACCTCTGCCCACTAACAAAATCCTTGACCTCAATCTATATAAGTTCAGTCCCATGCAACTCGCAGAGATGCACAAATTCCGGGAAGAGGAGGCAATGTACTTCTTCAGCCCAAGGGATCGGAAGTATCCGAATGGTAAGCTTCCGAGCCGCATAACTGGTGAAGGTGATGGATTATGGAAGGCAACTGGTAGAGACTTGGAAATTTTCAGTCAAAGGTGGAAGGTAATTGGAGCCAGGAAGACCCTGGTCTTTTATGATCTGAAATCAAAACATGGAGCAAAAAAGACCAATTGGATTATGCATGAATACACAATCGAGGAAAACATCTCACCATCCACAAGCACCACTAGAGCTCCTGGGGACAACAGGTTGGATGATTGGGTTCTGTGCAAGGTGTATCTAAGTAAGATGTCTAAAACTAACCCAAATACTGGGGACAAAGAGAAAACAGAGATGACAGATTCAGAGCCATTAGAGCAGGATCTTCGAGTGCTTGAAAGCTCTGCAGCTCCAAACATAGGTGAACAAGTTAACGTGAGCAATGAAAGTGTGCAGGCATTATACAATGGAATCATGACAGTACCGTATGGCTCTACTACGGGTTATGTGTCTCTAGAGGTGACAAATTTAGGTGCAAGGTCGCTTGCTCATCGTCAACAAAACCTTTCATTGCTACCAAGCTTTTCGCAGCTGCCAGTTATTAACAGTGTTAGCTCTGCTATTTCCAGCCTTGCTGGAGGAGACAACAATAATGGAACGATCACTTCACCATATGATGGTTCAAATGGATCTGAAAACGTGATAAATTTGCATCAAAACTTGCATGCAGATCATCAGGAAGACCTTCCATCACTTCAAAACTCTTCGAAGCCCACTGTTATTAACAGCGTTGGGTCTGCTATTGCCAGTCTTGCCGGCGGTCCTGTGTTTCCTGATTTCCAACAGGTTCCTCGTGATTTTGGAGCTCAATTTGGGACAAATATAGCTCCCATAAATGGAATCCAAGCATATCAGTCCCCTCGTGGAATTGGACCTCATCAATCTCGGCCAAGTTTGGACAACGGATGCCTCCCTCCATTACCGCAGTGTTTTTACTATTATGATGATCGAGGATATAGTTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

433

Amino Acids

48.17

Weight (kDa)

6.02

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 33 - 161 1.4e-35 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 602, 947
AciI CCGC 3 cut(s) 319, 1089, 1253
AclWI GGATC 3 cut(s) 294, 660, 967
AcoI YGGCCR 1 cut(s) 1217
AcsI RAATTY 4 cut(s) 245, 372, 796, 976
AcuI CTGAAG 1 cut(s) 259
AfaI GTAC 3 cut(s) 10, 269, 753
AfiI CCNNNNNNNGG 5 cut(s) 256, 602, 689, 947, 1166
AgsI TTSAA 6 cut(s) 116, 143, 166, 668, 954, 1028
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 3 cut(s) 404, 414, 526
Alw21I GWGCWC 2 cut(s) 526, 1138
Alw26I GTCTC 3 cut(s) 357, 786, 906
AlwI GGATC 3 cut(s) 294, 660, 967
AoxI GGCC 1 cut(s) 1217
ApeKI GCWGC 3 cut(s) 677, 859, 862
ApoI RAATTY 4 cut(s) 245, 372, 796, 976
Asp700I GAANNNNTTC 1 cut(s) 1014
AspS9I GGNCC 2 cut(s) 1091, 1202
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 6 cut(s) 341, 347, 492, 704, 802, 930
AsuII TTCGAA 1 cut(s) 1037
AvaII GGWCC 2 cut(s) 1091, 1202
BanII GRGCYC 2 cut(s) 526, 1138
BauI CACGAG 2 cut(s) 1122, 1191
BbsI GAAGAC 2 cut(s) 416, 1017
Bbv12I GWGCWC 2 cut(s) 526, 1138
BbvI GCAGC 3 cut(s) 689, 849, 871
BccI CCATC 4 cut(s) 332, 511, 941, 1027
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 3 cut(s) 406, 416, 528
BciVI GTATCC 1 cut(s) 306
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 3 cut(s) 357, 786, 906
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 4 cut(s) 12, 120, 519, 785
BfmI CTRYAG 1 cut(s) 675
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 4 cut(s) 319, 678, 860, 863
BlsI GCNGC 4 cut(s) 320, 679, 861, 864
Bme1390I CCNGG 4 cut(s) 251, 406, 416, 528
Bme18I GGWCC 2 cut(s) 1091, 1202
BmgT120I GGNCC 2 cut(s) 1091, 1202
BmiI GGNNCC 3 cut(s) 20, 403, 1119
BmrFI CCNGG 4 cut(s) 251, 406, 416, 528
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 3 cut(s) 228, 991, 1226
BmuI ACTGGG 1 cut(s) 612
BoxI GACNNNNGTC 1 cut(s) 416
BpiI GAAGAC 2 cut(s) 416, 1017
BpmI CTGGAG 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 1037
BpuMI CCSGG 1 cut(s) 251
BsaJI CCNNGG 4 cut(s) 98, 281, 414, 527
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 508, 538
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 602, 689, 947, 1166
Bse118I RCCGGY 1 cut(s) 1085
Bse1I ACTGG 5 cut(s) 331, 361, 607, 866, 1077
Bse3DI GCAATG 3 cut(s) 270, 718, 839
BseBI CCWGG 3 cut(s) 406, 416, 528
BseDI CCNNGG 4 cut(s) 98, 281, 414, 527
BseGI GGATG 3 cut(s) 503, 550, 1241
BseLI CCNNNNNNNGG 5 cut(s) 256, 602, 689, 947, 1166
BseMI GCAATG 3 cut(s) 270, 718, 839
BseNI ACTGG 5 cut(s) 331, 361, 607, 866, 1077
BseRI GAGGAG 2 cut(s) 272, 924
BseXI GCAGC 3 cut(s) 689, 849, 871
BsgI GTGCAG 1 cut(s) 743
BshFI GGCC 1 cut(s) 1219
BsiHKAI GWGCWC 2 cut(s) 526, 1138
BsiSI CCGG 2 cut(s) 250, 1086
BslFI GGGAC 5 cut(s) 204, 545, 620, 1159, 1171
BslI CCNNNNNNNGG 5 cut(s) 256, 602, 689, 947, 1166
BsmAI GTCTC 3 cut(s) 357, 786, 906
BsmFI GGGAC 5 cut(s) 204, 545, 620, 1159, 1171
BsnI GGCC 1 cut(s) 1219
Bsp119I TTCGAA 1 cut(s) 1037
Bsp1286I GDGCHC 2 cut(s) 526, 1138
Bsp143I GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
Bsp19I CCATGG 1 cut(s) 98
BspACI CCGC 3 cut(s) 319, 1089, 1253
BspANI GGCC 1 cut(s) 1219
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 20, 403, 1119
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 3 cut(s) 294, 660, 967
BspT104I TTCGAA 1 cut(s) 1037
BsrDI GCAATG 3 cut(s) 270, 718, 839
BsrFI RCCGGY 1 cut(s) 1085
BsrI ACTGG 5 cut(s) 331, 361, 607, 866, 1077
BssAI RCCGGY 1 cut(s) 1085
BssECI CCNNGG 4 cut(s) 98, 281, 414, 527
BssMI GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
BssSI CACGAG 2 cut(s) 1122, 1191
BssT1I CCWWGG 2 cut(s) 98, 281
Bst2BI CACGAG 2 cut(s) 1122, 1191
Bst2UI CCWGG 3 cut(s) 406, 416, 528
Bst4CI ACNGT 4 cut(s) 751, 756, 878, 1048
Bst6I CTCTTC 3 cut(s) 123, 249, 1039
BstBI TTCGAA 1 cut(s) 1037
BstC8I GCNNGC 4 cut(s) 726, 816, 996, 1087
BstDEI CTNAG 1 cut(s) 572
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 3 cut(s) 503, 550, 1241
BstKTI GATC 7 cut(s) 289, 430, 655, 933, 962, 1003, 1279
BstMAI GTCTC 3 cut(s) 357, 786, 906
BstMBI GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
BstMWI GCNNNNNNNGC 2 cut(s) 646, 1082
BstNI CCWGG 3 cut(s) 406, 416, 528
BstNSI RCATGY 1 cut(s) 998
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 4 cut(s) 249, 404, 414, 526
BstSFI CTRYAG 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 689, 849, 871
BstV2I GAAGAC 2 cut(s) 416, 1017
BstX2I RGATCY 2 cut(s) 652, 959
BstYI RGATCY 2 cut(s) 652, 959
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 1 cut(s) 1219
BtgI CCRYGG 1 cut(s) 98
BtsCI GGATG 3 cut(s) 503, 550, 1241
BtsI GCAGTG 1 cut(s) 1262
BtsIMutI CAGTG 3 cut(s) 883, 1044, 1262
Cac8I GCNNGC 4 cut(s) 726, 816, 996, 1087
CciI TCATGA 1 cut(s) 744
Cfr10I RCCGGY 1 cut(s) 1085
Cfr13I GGNCC 2 cut(s) 1091, 1202
Csp6I GTAC 3 cut(s) 9, 268, 752
CviAII CATG 6 cut(s) 99, 223, 443, 473, 745, 995
CviQI GTAC 3 cut(s) 9, 268, 752
DdeI CTNAG 1 cut(s) 572
DpnI GATC 7 cut(s) 288, 429, 654, 932, 961, 1002, 1278
DpnII GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
EaeI YGGCCR 1 cut(s) 1217
Eam1104I CTCTTC 3 cut(s) 123, 249, 1039
EarI CTCTTC 3 cut(s) 123, 249, 1039
Ecl136II GAGCTC 2 cut(s) 524, 1136
Eco130I CCWWGG 2 cut(s) 98, 281
Eco24I GRGCYC 2 cut(s) 526, 1138
Eco47I GGWCC 2 cut(s) 1091, 1202
Eco53kI GAGCTC 2 cut(s) 524, 1136
Eco57I CTGAAG 1 cut(s) 259
EcoICRI GAGCTC 2 cut(s) 524, 1136
EcoRII CCWGG 3 cut(s) 404, 414, 526
EcoT14I CCWWGG 2 cut(s) 98, 281
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 526, 1138
ErhI CCWWGG 2 cut(s) 98, 281
FaeI CATG 6 cut(s) 102, 226, 446, 476, 748, 998
FaqI GGGAC 5 cut(s) 204, 545, 620, 1159, 1171
FatI CATG 6 cut(s) 98, 222, 442, 472, 744, 994
FauNDI CATATG 2 cut(s) 73, 943
Fnu4HI GCNGC 4 cut(s) 319, 678, 860, 863
FokI GGATG 3 cut(s) 490, 557, 1248
FriOI GRGCYC 2 cut(s) 526, 1138
Fsp4HI GCNGC 4 cut(s) 319, 678, 860, 863
FspBI CTAG 4 cut(s) 12, 120, 519, 785
GluI GCNGC 4 cut(s) 319, 678, 860, 863
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 1 cut(s) 1219
HapII CCGG 2 cut(s) 250, 1086
Hin1II CATG 6 cut(s) 102, 226, 446, 476, 748, 998
HincII GTYRAC 2 cut(s) 703, 827
HindII GTYRAC 2 cut(s) 703, 827
HindIII AAGCTT 2 cut(s) 308, 850
HinfI GANTC 4 cut(s) 136, 632, 741, 1170
HpaI GTTAAC 1 cut(s) 703
HpaII CCGG 2 cut(s) 250, 1086
HphI GGTGA 6 cut(s) 341, 347, 492, 704, 802, 930
Hpy166II GTNNAC 3 cut(s) 695, 703, 827
Hpy188I TCNGA 6 cut(s) 291, 300, 315, 432, 637, 964
Hpy188III TCNNGA 7 cut(s) 58, 163, 745, 785, 1007, 1103, 1124
Hpy8I GTNNAC 3 cut(s) 695, 703, 827
HpyAV CCTTC 4 cut(s) 326, 343, 385, 1025
HpyCH4III ACNGT 4 cut(s) 751, 756, 878, 1048
HpyCH4IV ACGT 2 cut(s) 705, 969
HpyF10VI GCNNNNNNNGC 2 cut(s) 646, 1082
HpyF3I CTNAG 1 cut(s) 572
HpySE526I ACGT 2 cut(s) 705, 969
Hsp92II CATG 6 cut(s) 102, 226, 446, 476, 748, 998
KroI GCCGGC 1 cut(s) 1085
KroNI GCCGGC 1 cut(s) 1087
KspAI GTTAAC 1 cut(s) 703
Kzo9I GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
LmnI GCTCC 7 cut(s) 46, 401, 446, 529, 685, 1133, 1162
Lsp1109I GCAGC 3 cut(s) 689, 849, 871
LweI GCATC 3 cut(s) 228, 991, 1226
MaeI CTAG 4 cut(s) 12, 120, 519, 785
MaeII ACGT 2 cut(s) 705, 969
MaeIII GTNAC 1 cut(s) 790
MalI GATC 7 cut(s) 288, 429, 654, 932, 961, 1002, 1278
MboI GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
MboII GAAGA 8 cut(s) 131, 140, 265, 266, 421, 647, 1022, 1026
MfeI CAATTG 1 cut(s) 460
MflI RGATCY 2 cut(s) 652, 959
MhlI GDGCHC 2 cut(s) 526, 1138
MluCI AATT 9 cut(s) 34, 245, 372, 396, 460, 796, 976, 1139, 1197
MmeI TCCRAC 2 cut(s) 522, 1135
Mph1103I ATGCAT 1 cut(s) 474
MroNI GCCGGC 1 cut(s) 1085
MroXI GAANNNNTTC 1 cut(s) 1014
MseI TTAA 4 cut(s) 108, 702, 873, 1053
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 862
MspI CCGG 2 cut(s) 250, 1086
MspR9I CCNGG 4 cut(s) 251, 406, 416, 528
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 3 cut(s) 406, 416, 528
MwoI GCNNNNNNNGC 2 cut(s) 646, 1082
NaeI GCCGGC 1 cut(s) 1087
NciI CCSGG 1 cut(s) 251
NcoI CCATGG 1 cut(s) 98
NdeI CATATG 2 cut(s) 73, 943
NdeII GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
NgoMIV GCCGGC 1 cut(s) 1085
NlaIII CATG 6 cut(s) 102, 226, 446, 476, 748, 998
NlaIV GGNNCC 3 cut(s) 20, 403, 1119
NmeAIII GCCGAG 1 cut(s) 1195
NmuCI GTSAC 1 cut(s) 790
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 1 cut(s) 998
NspV TTCGAA 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 998
PagI TCATGA 1 cut(s) 744
PdiI GCCGGC 1 cut(s) 1087
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 4 cut(s) 136, 632, 741, 1170
PflMI CCANNNNNTGG 2 cut(s) 602, 947
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 4 cut(s) 320, 679, 861, 864
PshAI GACNNNNGTC 1 cut(s) 416
Psp124BI GAGCTC 2 cut(s) 526, 1138
Psp6I CCWGG 3 cut(s) 404, 414, 526
PspGI CCWGG 3 cut(s) 404, 414, 526
PspN4I GGNNCC 3 cut(s) 20, 403, 1119
PspPI GGNCC 2 cut(s) 1091, 1202
PstI CTGCAG 1 cut(s) 679
PsuI RGATCY 2 cut(s) 652, 959
PvuII CAGCTG 1 cut(s) 862
RsaI GTAC 3 cut(s) 10, 269, 753
RsaNI GTAC 3 cut(s) 9, 268, 752
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 2 cut(s) 526, 1138
SaqAI TTAA 4 cut(s) 108, 702, 873, 1053
SatI GCNGC 4 cut(s) 319, 678, 860, 863
Sau3AI GATC 7 cut(s) 286, 427, 652, 930, 959, 1000, 1276
Sau96I GGNCC 2 cut(s) 1091, 1202
ScrFI CCNGG 4 cut(s) 251, 406, 416, 528
SduI GDGCHC 2 cut(s) 526, 1138
SfaNI GCATC 3 cut(s) 228, 991, 1226
SfcI CTRYAG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 1037
SinI GGWCC 2 cut(s) 1091, 1202
SmiMI CAYNNNNRTG 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 11
SphI GCATGC 1 cut(s) 998
Sse9I AATT 9 cut(s) 34, 245, 372, 396, 460, 796, 976, 1139, 1197
SsiI CCGC 3 cut(s) 319, 1089, 1253
SspMI CTAG 4 cut(s) 12, 120, 519, 785
SstI GAGCTC 2 cut(s) 526, 1138
StyD4I CCNGG 4 cut(s) 249, 404, 414, 526
StyI CCWWGG 2 cut(s) 98, 281
TaaI ACNGT 4 cut(s) 751, 756, 878, 1048
TaiI ACGT 2 cut(s) 708, 972
TaqI TCGA 5 cut(s) 57, 486, 658, 1037, 1279
TasI AATT 9 cut(s) 34, 245, 372, 396, 460, 796, 976, 1139, 1197
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 4 cut(s) 136, 632, 741, 1170
Tru1I TTAA 4 cut(s) 108, 702, 873, 1053
Tru9I TTAA 4 cut(s) 108, 702, 873, 1053
TscAI CASTG 3 cut(s) 883, 1051, 1262
TseFI GTSAC 1 cut(s) 790
TseI GCWGC 3 cut(s) 677, 859, 862
Tsp45I GTSAC 1 cut(s) 790
TspDTI ATGAA 6 cut(s) 80, 141, 167, 489, 729, 828
TspGWI ACGGA 2 cut(s) 33, 1248
TspRI CASTG 3 cut(s) 883, 1051, 1262
Van91I CCANNNNNTGG 2 cut(s) 602, 947
VpaK11BI GGWCC 2 cut(s) 1091, 1202
XapI RAATTY 4 cut(s) 245, 372, 796, 976
XbaI TCTAGA 1 cut(s) 784
XceI RCATGY 1 cut(s) 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 1014
XspI CTAG 4 cut(s) 12, 120, 519, 785
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.